Objective:Growing problem of antibiotic resistance in Helicobacter pylori,as a common cause of chronic gastritis and even stomach cancer,demands searching for novel candidates of herbal sources.This study is aimed at ...Objective:Growing problem of antibiotic resistance in Helicobacter pylori,as a common cause of chronic gastritis and even stomach cancer,demands searching for novel candidates of herbal sources.This study is aimed at assessing the antimicrobial activity of aqueous extract obtained from Quercus brantii var.persica seed coat(Testa)on H.pylori isolated from gastric biopsy specimens.Methods:Such specimens were collected from 100 patients presenting with endoscopic gastroduodenal findings.Testa extracts were prepared from Persian Oak forests in the province of Kohgiluyeh and BoyerAhmad,IRAN.H.pylori isolates were obtained by a series of standard bacteriology tests and cell culture,then were confirmed by PCR.The activity of testa extracts towards 25 H.pylori isolates was assessed by well diffusion method,microdilution assay,and a disk diffusion assay in vitro.Results were analyzed statistically by one-way ANOVA analysis.Results:Aqueous extract of testa demonstrated an antimicrobial activity with zone diameters of inhibition ranged from 0 mm to 40 mm.Its inhibitory activity increased simultaneously with increasing extract concentration.The lowest MIC and MBC were both recorded as 2μg/m L.Anti-H.pylori activity of testa extract was approximately close to tetracycline and metronidazole and less than amoxicillin.A potent extract of testa possessed significant inhibitory activity(P<0.05).Conclusion:Testa extract is suggested as a natural therapeutic source against the gastric H.pylori infection.However,evaluating the in vivo activity of this extract is necessary too.展开更多
本文以健康婴儿粪便及光明乳业技术中心保藏菌种为筛选源,利用琼脂块扩散法和孔扩散法筛选得到5株对Hp具有较强抑制作用的乳酸菌,其中Bifidobacterium longum J16和Lactobacillus casei LC2W的抑菌效果最为明显。初步探讨了J16和LC2W两...本文以健康婴儿粪便及光明乳业技术中心保藏菌种为筛选源,利用琼脂块扩散法和孔扩散法筛选得到5株对Hp具有较强抑制作用的乳酸菌,其中Bifidobacterium longum J16和Lactobacillus casei LC2W的抑菌效果最为明显。初步探讨了J16和LC2W两株菌对Hp SS1的抑菌机理:J16和LC2W发酵MRS的上清液对Hp有较强抑制作用,但并非有机酸和H2O2所致;J16的抑菌成分粗提物对胃蛋白酶、胰蛋白酶、木瓜蛋白酶、热处理不敏感,但经蛋白酶K作用后抑菌作用增强;在酸性pH值范围内(pH1.5-4.0),J16和LC2W发酵乳对Hp的抑菌作用不会消失。展开更多
基金research grant support(MICRO_96_A2)from Yasuj University of Medical Sciencessupported by Yasuj University of Medical Sciences,Yasuj,Iran.
文摘Objective:Growing problem of antibiotic resistance in Helicobacter pylori,as a common cause of chronic gastritis and even stomach cancer,demands searching for novel candidates of herbal sources.This study is aimed at assessing the antimicrobial activity of aqueous extract obtained from Quercus brantii var.persica seed coat(Testa)on H.pylori isolated from gastric biopsy specimens.Methods:Such specimens were collected from 100 patients presenting with endoscopic gastroduodenal findings.Testa extracts were prepared from Persian Oak forests in the province of Kohgiluyeh and BoyerAhmad,IRAN.H.pylori isolates were obtained by a series of standard bacteriology tests and cell culture,then were confirmed by PCR.The activity of testa extracts towards 25 H.pylori isolates was assessed by well diffusion method,microdilution assay,and a disk diffusion assay in vitro.Results were analyzed statistically by one-way ANOVA analysis.Results:Aqueous extract of testa demonstrated an antimicrobial activity with zone diameters of inhibition ranged from 0 mm to 40 mm.Its inhibitory activity increased simultaneously with increasing extract concentration.The lowest MIC and MBC were both recorded as 2μg/m L.Anti-H.pylori activity of testa extract was approximately close to tetracycline and metronidazole and less than amoxicillin.A potent extract of testa possessed significant inhibitory activity(P<0.05).Conclusion:Testa extract is suggested as a natural therapeutic source against the gastric H.pylori infection.However,evaluating the in vivo activity of this extract is necessary too.
