Anti-CD86mAb干预哮喘患儿PBMC后对其产生Th源细胞因子影响的研究

目的 :探讨Th源细胞因子和CD86在哮喘发病中的作用及CD86mAb治疗哮喘的临床潜在价值。方法 :随机选择哮喘急性发作期患儿 2 8例 ,男 18例 ,女 10例 ,年龄 1岁～ 8 0 8岁 ,平均年龄 (4 83± 1 99)岁 ;对照组 15例 ,男 7例 ,女 8例 ,年龄 3 2 5岁～ 10岁 ,平均年龄 (6 6 3± 1 91)岁。ELISA检测PHA刺激并用小鼠抗人CD86mAb干预PBMC(peripheralbloodmononuclearcell,PBMC)后其培养上清IL 4、IL 13、IFN γ水平。结果 :小鼠抗人CD86mAb(5× 10 - 3μg ml)干预PBMC后 ,正常组和哮喘组IL 4、IL 13和IFN γ水平均降低 ,与相应小鼠IgG对照组相比差异有显著性 ,而哮喘组IL 4和IL 13降低更明显。结论 :CD86mAb的阻断是非特异性的 ,即对Th1和Th2细胞均有一定的抑制作用 ,但对Th2细胞的抑制作用尤甚 ,说明CD86信号传导在哮喘的发生和发展过程中起重要作用。

CXCL-13抗体对自身免疫疾病治疗的研究进展

B淋巴细胞趋化因子-1(CXCL-13)通过趋化作用、内化作用等特性,可能与自身免疫性疾病及炎症性疾病中多种疾病的发病机制有关。CXCL-13作用过程中,与其受体中和可能干扰靶器官中异常生发中心的形成,并且阻止自身免疫疾病的发生、发展,因此,提出一种新的治疗自身免疫疾病的靶点-CXCL-13抗体。本文对CXCL-13信号通路为靶点的抗体-MAb 5261治疗自身免疫性疾病的新型疗法的研究进展进行综述。

抗白介素-13单克隆抗体生物学活性测定方法的建立

目的:建立抗白介素-13单克隆抗体(抗IL-13单抗)的生物学活性检测方法。方法:利用L-BEAS-2B细胞系,通过荧光素酶检测系统进行抗IL-13单抗的生物学活性检测,通过抗IL-13单抗参比品与样品对比以平行线分析的方法计算样品相对百分效价,并对该方法的精密性和准确性进行验证。结果:抗IL-13单抗在该方法中存在量效关系,在半对数坐标纸上呈平行的直线分布。6批抗IL-13单抗样品经3次测定,相对百分效价平均值在(91.10±1.10)%~(105.27±7.17)%之间,RSD均小于12%;1批样品经9次测定平均相对百分效价为(110.77±7.01)%,板间和日间RSD均小于7%;2批回收率样品经3次测定,回收率分别为(96.17±8.50)%和(91.53±15.46)%。结论:研究建立的抗IL-13单抗生物学活性检测方法准确性高,精密度及重复性好,可作为抗IL-13单抗生物学活性的常规检测方法。

Study of effects of anti-IL-10 monoclonal antibody on systemicCandidiasis

Objective:To investigate the effects of anti-interleukin-10 monoclonal antibody(anti-IL-10MAb) on systemic candidiasis.Methods:Control group(only candidiasis injection),and disposal group(candidiasis infection accompanying with anti-IL-10MAb infection) of cyclophosphamide-induced immuno-suppressed murine systemic candidiasis model were set in this study. Colony Forming Units(CFUs) of infected kidneys and spleens were determined using plating dilution method. The histological studies for infected livers,spleens,and kidneys were applied. Levels of interferon gamma(IFN-γ) in spleen tissue homogenare were also measured by enzyme-linked immunosorbent assay.Results: In kidneys, the numbers of CFU of the disposal group were much lower than that of the control group; the numbers of CFU in spleens were similar to the control group. The histopathological scores of the disposal group were much better than that of the control group in kindneys with significant differences(P<0.01). In spleens,the histopathological scores of disposal group were also better than that of control group,but no statistic significant differences were observed (P>0.05). And the spleen IFN-γ level of the disposal group was significant higher than that of the control group(P<0.01). Conclusion: Anti-IL-10MAb effects on systemic candidiasis was concluded.