Comparison between Four Types of Buffers for DNA Agarose Gel Electrophoresis

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, electrophoresis buffers were used widely to separate any DNA molecule. In this paper, we used four types of previously known electrophoresis buffers to compare which is easy for preparation, simple in structure, low cost and good performance in agarose gel electrophoresis. For this, we used two agarose concentration (1%, 2%) and two types of DNA ladder (100 bp, 1 kb) represent both smaller and larger sizes of molecule for each type of buffers, from the result we found in first level both supper buffer and TAE buffer with good performance and in second level we found bicarbonate buffer also with good performance also. Finally, we found the tang buffer cannot pose any electrophoretic activity on DNA agarose gel electrophoresis.

Wedge-shaped HfO_(2) buffer layer-induced field-free spin-orbit torque switching of HfO_(2)/Pt/Co structure

Field-free spin-orbit torque(SOT)switching of perpendicular magnetization is essential for future spintronic devices.This study demonstrates the field-free switching of perpendicular magnetization in an HfO_(2)/Pt/Co/TaO_(x) structure,which is facilitated by a wedge-shaped HfO_(2)buffer layer.The field-free switching ratio varies with HfO_(2)thickness,reaching optimal performance at 25 nm.This phenomenon is attributed to the lateral anisotropy gradient of the Co layer,which is induced by the wedge-shaped HfO_(2)buffer layer.The thickness gradient of HfO_(2)along the wedge creates a corresponding lateral anisotropy gradient in the Co layer,correlating with the switching ratio.These findings indicate that field-free SOT switching can be achieved through designing buffer layer,offering a novel approach to innovating spin-orbit device.

Impact response and energy absorption of metallic buffer with entangled wire mesh damper

An innovative metallic buffer consisting of series-connected hat-shaped entangled wire mesh damper(EWMD)and parallel springs are proposed in this work to enhance the reliability of engineering equipment.The impact response and the energy dissipation mechanism of hat-shaped EWMD under different quasi-static compression deformations(2-7 mm)and impact heights(100-200 mm)are investigated using experimental and numerical methods.The results demonstrate distinct stages in the quasi-static mechanical characteristics of hat-shaped EWMD,including stiffness softening,negative stiffness,and stiffness hardening.The loss factor gradually increases with increasing compression deformation before entering the stiffness hardening stage.Under impact loads,the hat-shaped EWMD exhibits optimal impact energy absorption when it enters the negative stiffness stage(150 mm),resulting in the best impact isolation effect of metallic buffer.However,the impact energy absorption significantly decreases when hat-shaped EWMD enters the stiffness hardening stage.Interestingly,quasi-static compression analysis after experiencing different impact loads reveals the disappearance of the negative stiffness phenomenon.Moreover,with increasing impact loads,the stiffness hardening point progressively shifts to an earlier stage.

Irrigation and Thermal Buffering Using Mathematical Modeling

Two methods of irrigation,drip,and sprinkler were studied to determine the response of the Javits green roof to irrigation.The control study was dry unirrigated plots.Drip irrigation consisted of irrigation tubes running through the green roof that would water the soil throughout and sprinkler irrigation used a sprinkler system to irrigate the green roof from above.In all cases,the irrigated roofs had increased the soil moisture,reduced temperatures of both the upper and lower surfaces,reduced growing medium temperatures and reduced air temperatures above the green roof relative to the unirrigated roof.The buffered temperature fluctuations were also studied via air conditioner energy consumption.There was a 28%reduction in air conditioner energy consumption and a 33%reduction in overall energy consumption between dry and irrigated plots.Values of thermal resistance or S were determined for accuracy and for this study,there was little change which is ideal.A series of infra-red and thermal probe measurements were used to determine temperatures in the air and sedum.It was determined that the sprinkler irrigation did a better job than the drip irrigation in keeping cooler temperatures within the green roof.A Mann-Whitney U test was performed to verify the variation in moisture temperatures buffering energy consumption.By getting a p-value<0.05,it indicates that the model is accurate for prediction and medium temperatures were statistically different.

Non-destructive buffer enabling near-infrared-transparent inverted inorganic perovskite solar cells toward 1400 h light-soaking stable perovskite/Cu(In,Ga)Se_(2) tandem solar cells

Near-infrared(NIR)transparent inverted all-inorganic perovskite solar cells(PSCs)are excellent top cell candidates in tandem applications.An essential challenge is the replacement of metal contacts with transparent conductive oxide(TCO)electrodes,which requires the introduction of a buffer layer to prevent sputtering damage.In this study,we show that the conventional buffers(i.e.,small organic molecules and atomic layer deposited metal oxides)used for organic-inorganic hybrid perovskites are not applicable to all-inorganic perovskites,due to non-uniform coverage of the vulnerable layers underneath,deterioration upon ion bombardment and moisture induced perovskite phase transition,A thin film of metal oxide nanoparticles by the spin-coating method serves as a non-destructive buffer layer for inorganic PSCs.All-inorganic inverted near-infrared-transparent PSCs deliver a PCE of 17.46%and an average transmittance of 73.7%between 780 and 1200 nm.In combination with an 18.56%Cu(In,Ga)Se_(2) bottom cell,we further demonstrate the first all-inorganic perovskite/CIGS 4-T tandem solar cell with a PCE of 24.75%,which exhibits excellent illumination stability by maintaining 86.7%of its initial efficiency after 1400 h.The non-destructive buffer lays the foundation for efficient and stable NIR-transparent inverted inorganic perovskite solar cells and perovskite-based tandems.

Numerical Study and Optimization of CZTS-Based Thin-Film Solar Cell Structure with Different Novel Buffer-Layer Materials Using SCAPS-1D Software

This study explored the performances of CZTS-based thin-film solar cell with three novel buffer layer materials ZnS, CdS, and CdZnS, as well as with variation in thickness of buffer and absorber-layer, doping concentrations of absorber-layer material and operating temperature. Our aims focused to identify the most optimal thin-film solar cell structure that offers high efficiency and lower toxicity which are desirable for sustainable and eco-friendly energy sources globally. SCAPS-1D, widely used software for modeling and simulating solar cells, has been used and solar cell fundamental performance parameters such as open-circuited voltage (), short-circuited current density (), fill-factor() and efficiency() have been optimized in this study. Based on our simulation results, it was found that CZTS solar cell with Cd0.4Zn0.6S as buffer-layer offers the most optimal combination of high efficiency and lower toxicity in comparison to other structure investigated in our study. Although the efficiency of Cd0.4Zn0.6S, ZnS and CdS are comparable, Cd0.4Zn0.6S is preferable to use as buffer-layer for its non-toxic property. In addition, evaluation of performance as a function of buffer-layer thickness for Cd0.4Zn0.6S, ZnS and CdS showed that optimum buffer-layer thickness for Cd0.4Zn0.6S was in the range from 50 to 150nm while ZnS offered only 50 – 75 nm. Furthermore, the temperature dependence performance parameters evaluation revealed that it is better to operate solar cell at temperature 290K for stable operation with optimum performances. This study would provide valuable insights into design and optimization of nanotechnology-based solar energy technology for minimizing global energy crisis and developing eco-friendly energy sources sustainable and simultaneously.

Protocol Buffers在即时通讯系统中的应用研究

即时通讯系统已经广泛应用在人们的生活中,随着用户数量的增长,计算机设备在网络中传输的数据量越来越大。由于网络中的数据传输是非结构化的,因此需要数据的序列化与逆序列化方法来实现网络数据到可读数据的转换。基于即时通讯系统对于数据的实时性需求,提出了使用Protocol Buffers作为系统的序列化与逆序列化的手段,并且将Protocol Buffers与目前几种比较主流的数据序列化方法进行了对比。

Timing Optimization by Inserting Minimum Buffers with Accurate Delay Models

An algorithm of minimizing the number of buffers under certain delay constraint with accurate delay models is presented.Given a two-terminal net,the algorithm can minimize the total number of buffers inserted to meet the delay constraint.A high order delay model is applied to estimate interconnect delay and a nonlinear delay model based on look-up table is for buffer delay estimation.The experimental results show that the algorithm can efficiently achieve the trade-offs between number of buffers and delay,and avoid needless power and area cost.The running time is satisfactory.

BUFFERS的优化设置

设置BUFFERS数目并减少常规内存占用的技巧。

Protocol Buffers——比XML快近100倍

文章介绍了Google公司开发的一种新的数据描述语言—Protocol Buffers,说明了Protocol Buffers的定义、来源、发展和特点,且对Protocol Buffers和XML进行了比较,阐述了Protocol Buffers是如何工作的。

基于Protocol Buffers的配置下发接口适配与应用

简述了网管接口技术的发展现状,对现有网管接口技术使用的编码方式做出了比较;介绍了一种简单快速的序列化机制Protocol Buffers的基本功能与特点;提出了一种采用Protocol Buffers作为编码方式的网管配置下发模型,设计了一个Protocol Buffers与现有命令行(CLI)接口进行适配的适配层,给出了其工作原理与部署方法。

Protocol Buffers在数据采集与传输系统中的应用

随着传感器类型的多样化,采集和传输的数据类型也越来越多样化。这也要求数据采集与传输系统能够方便快捷的对不同类型的传感器和传输数据类型进行扩展。在基于Protocol Buffers的数据采集与传输系统中,通过使用Protocol Buffers作为数据序列化和反序列化的手段,并利用其反射机制等实现系统采集数据类型的快速扩展。

Increase of brightness and transmission efficiency in fiat panel display through serial synchronous scanning mode with double buffers

This paper presents a serial synchronous scanning mode in fiat panel display （FPD） by adding a latch buffer between the serializer and the driving buffer. Comparing with conventional techniques, the proposed structure can efficiently reduce the brightness loss and improve the transmission performance. Theoretical analysis and experimental results show that the ratio between the lightest weight display time and the relative transmission time is a tradeoff between brightness loss and transmission efficiency.

0.6-eV bandgap In_(0.69)Ga_(0.31)As thermophotovoltaic devices with compositionally undulating step-graded InAsyP_(1-y)buffers

Single-junction,lattice-mismatched In0.69Ga0.31As thermophotovoltaic（TPV） devices each with a bandgap of 0.6 eV are grown on InP substrate by metal-organic chemical vapour deposition（MOCVD）.Compositionally undulating stepgraded InAsyP1-y buffer layers with a lattice mismatch of ～1.2% are used to mitigate the effect of lattice mismatch between the device layers and the InP substrate.With an optimized buffer thickness,the In0.69Ga0.31As active layers grown on the buffer display a high crystal quality with no measurable tetragonal distortion.High-performance single-junction devices are demonstrated,with an open-circuit voltage of 0.215 V and a photovoltaic conversion efficiency of 6.9% at a short-circuit current density of 47.6 mA/cm2,which are measured under the standard solar simulator of air mass 1.5-global（AM 1.5 G）.

Load-to-store:store buffer暂态窗口时间泄露的利用

为了研究现代处理器微架构中的漏洞并制定对应防护,针对负责管理访存指令执行顺序的内存顺序缓冲(MOB)进行分析,发现前向加载会把存在依赖的store指令的数据直接旁路到load指令,推测加载会提前执行不存在依赖的load指令,在带来效率优化的同时,也可能导致执行出错与相应的阻塞。针对Intel Coffee Lake微架构上现有MOB优化机制,分析如何利用内存顺序缓冲的4种执行模式与对应执行时间,构造包括暂态攻击、隐蔽信道与还原密码算法私钥的多种攻击。利用MOB引发的时间差还原内存指令地址,该地址可泄露AES T表实现的索引值。在Intel i5-9400处理器上对OpenSSL 3.0.0的AES-128进行了密钥还原实验,实验结果显示,30000组样本能以63.6%概率还原出一个密钥字节,且由于内存顺序缓冲的特性,该利用隐蔽性优于传统cache时间泄露。

Landscape fragmentation associated with the Qingzang Highway and its influencing factors-A comparison study on road sections and buffers

Many studies have examined the effect of roads on landscape fragmentation.Yet they rarely considered local characteristics of the road and road buffer widths.Therefore,this study that took place in the Qingzang Highway(QH)examined the variations in road buffers and road sections of landscape fragmentation.The QH was divided into 32 sections with 23 buffer areas.Based on the indicators of landscape fragmentation from 1980 to 2018,we found significant spatial heterogeneity between sections and buffers.Generally,landscape fragmentation de-creased with increasing buffer distance to the QH.For different sections,the coefficients of variation between buffers were rather high and significantly different.Therefore,fixed-width buffers may overestimate or underes-timate the spatial scope and influence intensity of a road.The impacts of road sections around provincial capitals,prefecture-level cities and main counties on landscape fragmentation were relatively extensive and formed clus-ters of highly fragmented areas.Geodetector results indicated that natural and anthropogenic factors,such as altitude,climate,distance to major settlements and socioeconomic conditions,could well explain the spatiotem-poral characteristics of landscape fragmentation.Altitude,precipitation and the distance to major settlements had higher explanatory power for landscape fragmentation in permafrost regions,whereas slope and socioeconomic condition had higher explanatory power for non-permafrost regions in Xizang Autonomous Region.

An effective discrete artificial bee colony algorithm for flow shop scheduling problem with intermediate buffers

An effective discrete artificial bee colony(DABC) algorithm is proposed for the flow shop scheduling problem with intermediate buffers(IBFSP) in order to minimize the maximum completion time(i.e makespan). The effective combination of the insertion and swap operator is applied to producing neighborhood individual at the employed bee phase. The tournament selection is adopted to avoid falling into local optima, while, the optimized insert operator embeds in onlooker bee phase for further searching the neighborhood solution to enhance the local search ability of algorithm. The tournament selection with size 2 is again applied and a better selected solution will be performed destruction and construction of iterated greedy(IG) algorithm, and then the result replaces the worse one. Simulation results show that our algorithm has a better performance compared with the HDDE and CHS which were proposed recently. It provides the better known solutions for the makespan criterion to flow shop scheduling problem with limited buffers for the Car benchmark by Carlier and Rec benchmark by Reeves. The convergence curves show that the algorithm not only has faster convergence speed but also has better convergence value.

SYSTEM AGGREGATION METHOD FOR FAILURE PRONE PRODUCTION LINES WITH UNRELIABLE LIMITED BUFFERS

Different from traditional aggregation method, the unreliable buffers are originally considered and a more general aggregation method is offered, in which not only the unreliable buffers are considered, but also the probabilities of system states are obtained by a discrete model rather than the continuous flow model of unreliable manufacturing systems. The solution technique is offered to get the system sate probabilities. The method advances the traditional system aggregation techniques. Numerical results specify the extended aggregation method and also show that the unreliable limited buffers have a strong impact on the efficiency of the production lines.

Growth and Characterization of Doped CeO_2 Buffers on Ni-W Substrates for Coated Conductors Using Metal Organic Deposition Method

CeO2 and Ce0.8M0.2O2-d films （M = Mn, Y, Gd, Sm, Nd and La） with （00l） preferred orientation have been prepared on biaxially textured Ni-W substrates by metal organic decomposition （MOD） method. The factors influencing the formation of cracks on the surface of these CeO2 and doped CeO2 films on Ni-W substrates were explored by X-ray diffraction （XRD）, scanning electron microscopy （SEM） analysis, atomic force microscopy （AFM） and differential scanning calorimetry （DSC）. The results indicate that many factors, such as the change of the ionic radii of doping cations, the transformation of crystal structure and the formation of oxygen vacancies in lattices at high annealing temperature, may be related to the formation of cracks on the surface of these films. However, the crack formation shows no dependence on the crystal lattice mismatch degree of the films with Ni-W substrates. Moreover, the suppression of surface cracks is related to the change of intrinsic elasticity of CeO2 film with doping of cations with a larger radius. SEM and AFM investigations of Ce0.8M0.2O2-d （M = Y, Gd, Sm, Nd and La） films reveal the dense, smooth and crack-free microstructure, and their lattice parameters match well with that of YBCO, illuminating that they are potentially suitable to be as buffer layer, especially as cap layer in multi-layer architecture of buffer layer for coated conductors.

PARASITIC TOLERANT DIFFERENTIAL SC TRANSCONDUCTANCE USING UNITY GAIN BUFFERS

The unity gain buffer will be good to design high frequency SCF if its resistiveeffects can be eliminated,and therefore the whole parasitic sensitivities will greatly be reduced.On the basis of this concept,a novel parasitic tolerant SC DTE(differential transconductanceelement)is proposed.SC floating inductor and integrator fit for high frequency applications areformed by the DTE.The computer simulation and experiment on a third order elliptic LP filterverify its validity.