Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medici...Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medicinal(TCM) plants are in practice for dengue fever in dengue endemic regions. These traditional medicines persevere with treatments, which modern medicines lack. The study aims to substantiate the anti-dengue potential of some traditional herbs and make them available for further studies to facilitate TCM users. Twelve TCM plants aqueous extracts were evaluated, which are described as cool herbs used for the diseases with high fever. Lead plants were established through detailed in vitro foci forming unit reduction analysis(FFURA) against all four serotypes and validated through quantitative real-time RT-PCR(q RT-PCR). Four plants potentially inhibited the virus in primary phenotypic in vitro evaluation. Two lead plants Dryopteris crassirhizoma(DC) and Morus alba(MA) were identified with half minimal inhibitory concentration(IC50) 130 and 221 μg m L^-1, respectively, while the selectivity indices(SI) were 4.21 and 4.62, respectively. Lead plants equally inhibited all four serotypes of DENV. Time-of-addition analysis suggested that, DC was active at later stages of viral replication, whereas MA was active during the early stages and even showed some prophylactic activity. Liquid chromatography-mass spectrometry(HPLC/MS) analysis revealed presence of flavonoids. DC and MA are identified as potential anti-dengue plants, active against varied stages of dengue virus replication cycle. These results may serve as the base knowledge for further investigation on their combined treatments or integrative treatment with western medicines, which may improve the overall anti-dengue activity in future.展开更多
Dengue is the most prevalent arthropod-borne viral diseases in terms of morbidity and mortality in the recent decade with the classic symptoms of high fever,headache as well as muscle and joint pain.Nilavembu Kudineer...Dengue is the most prevalent arthropod-borne viral diseases in terms of morbidity and mortality in the recent decade with the classic symptoms of high fever,headache as well as muscle and joint pain.Nilavembu Kudineer(NK)is one among the 32 types of internal medicines described in the ancient Siddha system of medicine in south India which reduces fever and relieves body aches.NK,a polyherbal formulation made up of eight traditional herbs in equal parts,is also recommended for prevention and management of all types of viral infections including Dengue/Chikugunya.Exploration of bioactive compounds in the plants is the primary step for the standardization and bioactivity screening of plants and formulations.So the current study aims at recording the chemical constituents and medicinal activities of each ingredient of NK.The anti-pyretic,anti-inflammatory,analgesic and immunostimulant effects of NK have been attributed to more than one ingredient in NK.The medicinal property of the NK can be also attributed to the combined effect of all phytochemicals present in these eight herbs.The current study encompasses the various chemicals and the activities of individual herbs but a thorough stereoscopic and chromatographic investigation is required to trace out the major phytochemical entities which are retained once the NK is prepared by the traditional methods.展开更多
基金the Universiti Tunku Abdul Rahman,Malaysia,in providing funding to this project through UTARSRF strategic research funding scheme IPRS/RMC/UTARSRF/PROGRAMME 2014-C1/007。
文摘Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medicinal(TCM) plants are in practice for dengue fever in dengue endemic regions. These traditional medicines persevere with treatments, which modern medicines lack. The study aims to substantiate the anti-dengue potential of some traditional herbs and make them available for further studies to facilitate TCM users. Twelve TCM plants aqueous extracts were evaluated, which are described as cool herbs used for the diseases with high fever. Lead plants were established through detailed in vitro foci forming unit reduction analysis(FFURA) against all four serotypes and validated through quantitative real-time RT-PCR(q RT-PCR). Four plants potentially inhibited the virus in primary phenotypic in vitro evaluation. Two lead plants Dryopteris crassirhizoma(DC) and Morus alba(MA) were identified with half minimal inhibitory concentration(IC50) 130 and 221 μg m L^-1, respectively, while the selectivity indices(SI) were 4.21 and 4.62, respectively. Lead plants equally inhibited all four serotypes of DENV. Time-of-addition analysis suggested that, DC was active at later stages of viral replication, whereas MA was active during the early stages and even showed some prophylactic activity. Liquid chromatography-mass spectrometry(HPLC/MS) analysis revealed presence of flavonoids. DC and MA are identified as potential anti-dengue plants, active against varied stages of dengue virus replication cycle. These results may serve as the base knowledge for further investigation on their combined treatments or integrative treatment with western medicines, which may improve the overall anti-dengue activity in future.
文摘Dengue is the most prevalent arthropod-borne viral diseases in terms of morbidity and mortality in the recent decade with the classic symptoms of high fever,headache as well as muscle and joint pain.Nilavembu Kudineer(NK)is one among the 32 types of internal medicines described in the ancient Siddha system of medicine in south India which reduces fever and relieves body aches.NK,a polyherbal formulation made up of eight traditional herbs in equal parts,is also recommended for prevention and management of all types of viral infections including Dengue/Chikugunya.Exploration of bioactive compounds in the plants is the primary step for the standardization and bioactivity screening of plants and formulations.So the current study aims at recording the chemical constituents and medicinal activities of each ingredient of NK.The anti-pyretic,anti-inflammatory,analgesic and immunostimulant effects of NK have been attributed to more than one ingredient in NK.The medicinal property of the NK can be also attributed to the combined effect of all phytochemicals present in these eight herbs.The current study encompasses the various chemicals and the activities of individual herbs but a thorough stereoscopic and chromatographic investigation is required to trace out the major phytochemical entities which are retained once the NK is prepared by the traditional methods.
