The rusion or IL-2 to anti-id mAb MK2-23 which bears the internal image or human highmolecular weight melanoma-associated antigen (HMW-MAA) was used to immunize a group ofBALB/c mice. The results demonstrate that the ...The rusion or IL-2 to anti-id mAb MK2-23 which bears the internal image or human highmolecular weight melanoma-associated antigen (HMW-MAA) was used to immunize a group ofBALB/c mice. The results demonstrate that the rusion protein is capable or eliciting significant levelsof specific Abs against HMW-MAA without use of carrier proteins or adjuvants. The rusion proteinwith or without conjugating to KLR appear to be uniqu, in its ability to induce high titers of anti-anti-idAbs, whereas the mAb MK2-23 in combination with IL-2 did not. We conclude that anti-idMK2-23-IL-2 fusion protein is a potential immunogeu eliciting significant anti-tumor immunity.展开更多
在RFID网络通信中,当多个标签同时回应阅读器的查询时,如果没有相应的防冲突机制,会导致标签到阅读器的通信冲突,使得从标签返回的数据难以被阅读器正确识别.防冲突算法是阅读器快速、正确获取标签数据的关键.一种被称为基于栈的ID-二...在RFID网络通信中,当多个标签同时回应阅读器的查询时,如果没有相应的防冲突机制,会导致标签到阅读器的通信冲突,使得从标签返回的数据难以被阅读器正确识别.防冲突算法是阅读器快速、正确获取标签数据的关键.一种被称为基于栈的ID-二进制树防冲突算法(Stack-based ID-binary tree anti-collision algorithm,SIBT)被提出,SIBT算法的新颖性在于它将n个标签的ID号映射为一棵唯一对应的ID-二进制树,标签识别过程转化为在阅读器中创建ID-二进制树的过程.为了提高多标签识别效率,阅读器使用栈保存已经获取的ID-二进制树创建线索,用计数器保存标签在该栈中的深度.理论分析和仿真结果表明SIBT算法的性能优于其他基于树的防冲突算法.展开更多
Wistar rats were inoculated with purified YWK-I antibody. The anti-idiotypie antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs. Specifi...Wistar rats were inoculated with purified YWK-I antibody. The anti-idiotypie antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs. Specificity of anti-Id antibody was established by ELISA. The 84 kD protein inhibited the binding of anti-Id to YWK-I mAb, but failed to repress antibody against normal mouse Ig binding to YWK-I mAb. In competitive inhibition assay, 84 kD protein had shown the ability to compete with anti-Id binding to YWK-I mAb in a dose-dependent manner. Crude sperm extract showed a lower competitive ability. No effeet was found with the irrelevant 36 kD sperm protein. The antisera from the Ba1b/e mice immunized with AId contained Ab3 that reacted with 84 kD sperm protein. The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sparm agglutination. These results indicate that anti-Id which may mimio an epitope of the 84 kD protein could be exploited as an antigen to raise antibodies against sperm protein.展开更多
In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(D...In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(DNR) to form SM6:Dex: DNR immunoconjugate.The activity of anti-Id-McAb and DNR of the immunoconjugate and their extent of the substitution were measured,and the autitumor activity of SM6:hex:DNR was assayed in vitro.The results demonstrateil that using Dex-T40 to prepare immunoconjugate could get a higher extent of substitution of McAb and DNR and maintain the activity of McAb and drug well.The results of cytotoxic assay in vitro suggested that the immunoconjugate SM6:Dex:DNR possess a specific cytotoxic effett to target tumor cells.展开更多
文摘The rusion or IL-2 to anti-id mAb MK2-23 which bears the internal image or human highmolecular weight melanoma-associated antigen (HMW-MAA) was used to immunize a group ofBALB/c mice. The results demonstrate that the rusion protein is capable or eliciting significant levelsof specific Abs against HMW-MAA without use of carrier proteins or adjuvants. The rusion proteinwith or without conjugating to KLR appear to be uniqu, in its ability to induce high titers of anti-anti-idAbs, whereas the mAb MK2-23 in combination with IL-2 did not. We conclude that anti-idMK2-23-IL-2 fusion protein is a potential immunogeu eliciting significant anti-tumor immunity.
文摘在RFID网络通信中,当多个标签同时回应阅读器的查询时,如果没有相应的防冲突机制,会导致标签到阅读器的通信冲突,使得从标签返回的数据难以被阅读器正确识别.防冲突算法是阅读器快速、正确获取标签数据的关键.一种被称为基于栈的ID-二进制树防冲突算法(Stack-based ID-binary tree anti-collision algorithm,SIBT)被提出,SIBT算法的新颖性在于它将n个标签的ID号映射为一棵唯一对应的ID-二进制树,标签识别过程转化为在阅读器中创建ID-二进制树的过程.为了提高多标签识别效率,阅读器使用栈保存已经获取的ID-二进制树创建线索,用计数器保存标签在该栈中的深度.理论分析和仿真结果表明SIBT算法的性能优于其他基于树的防冲突算法.
文摘Wistar rats were inoculated with purified YWK-I antibody. The anti-idiotypie antibodies were isolated from rat sera by successive passage over affinity chromatography columns of YWK-I mAb and normal mouse Igs. Specificity of anti-Id antibody was established by ELISA. The 84 kD protein inhibited the binding of anti-Id to YWK-I mAb, but failed to repress antibody against normal mouse Ig binding to YWK-I mAb. In competitive inhibition assay, 84 kD protein had shown the ability to compete with anti-Id binding to YWK-I mAb in a dose-dependent manner. Crude sperm extract showed a lower competitive ability. No effeet was found with the irrelevant 36 kD sperm protein. The antisera from the Ba1b/e mice immunized with AId contained Ab3 that reacted with 84 kD sperm protein. The binding of anti-Id to YWK-I mAb was inhibited by Ab3 in a dose-dependent fashion and Ab3 was shown to be able to induce human sparm agglutination. These results indicate that anti-Id which may mimio an epitope of the 84 kD protein could be exploited as an antigen to raise antibodies against sperm protein.
文摘In this experiment,the Dcxtran-T40(Dex-T40) served as intermidiate carrier,conjugated SM6 monoclonal antiidiotypic antibody(anti-Id-McAb) against B lymphocytic leukemia cells and chemotherapeutical drug daunorubicin(DNR) to form SM6:Dex: DNR immunoconjugate.The activity of anti-Id-McAb and DNR of the immunoconjugate and their extent of the substitution were measured,and the autitumor activity of SM6:hex:DNR was assayed in vitro.The results demonstrateil that using Dex-T40 to prepare immunoconjugate could get a higher extent of substitution of McAb and DNR and maintain the activity of McAb and drug well.The results of cytotoxic assay in vitro suggested that the immunoconjugate SM6:Dex:DNR possess a specific cytotoxic effett to target tumor cells.
