Here,we discuss an important problem in medicine as development of efctive strategies for brain drug delivery.This problem is related to the blood-brain barrier(BBB),which is a“customs”controlling the entrance of di...Here,we discuss an important problem in medicine as development of efctive strategies for brain drug delivery.This problem is related to the blood-brain barrier(BBB),which is a“customs”controlling the entrance of different molecules from blood into the brain protecting the normal function of central nervous system(CNS).We show three interfaces of anatomical side of BBB and two functional types of BBB一physical and transporter barriers.Although this protective mechanism is essential for health of CNS,it also creates a hindrance to the entry of drugs into the brain.The BBB was discovered over 100 years ago but till now,there is no efective methods for brain drug delivery.There ane more than 70 approaches for overcoming BBB incuding physical,chenical and biological techniques but all of these tools have limitation to be widely used in clinical practice due to invasi venes,challenge in performing,very costly or lim-itation of drug concentration.Photodynamic therapy(PDT)is usual clinical method of surgical navigation for the resection of brain tumor and anti-cancer therapy.Nowadays,the application of PDT is considered as a potential promising tool for brain drug delivery via opening of BBB.Here,we show the first sucoessful experimental results in this field discussing the adventures and disadv antages of PDT-related BBB disruption as well as altematives to overcome these limitations and possi ble mechanisms with new pathways for brain clearance via gly mphatic and lymphatic systems.展开更多
[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and pho...[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and photodynamic antitumor effect on rat glioma C6 cells. [Methods]The absorption spectrum and fluorescence spectrum of sodium chlorophyllin were measured by an ultraviolet spectrophotometer and a fluorescence spectrophotometer;the plate count method was used to investigate the photodynamic sterilization efficiency of sodium chlorophyllin on Escherichia coli and Staphylococcus aureus;and the MTT method was used to determine the photodynamic antitumor effect of sodium chlorophyllin on rat glioma cell C6. [Results] The sterilization rates of sodium chlorophyllin with the 100 J/cm^2 photodynamic treatment were 98.96% 1.284 and 100% 0 on S. aureus and E. coli, respectively. The half maximal inhibitory concentration(IC50) of the sodium chlorophyllin photodynamic therapy with 5, 10 and 20 J/cm^2 on C6 were 69.9, 48.21 and 47.56 μg/ml respectively, and the dark toxicity was extremely low at 0 J/cm^2. [Conclusions]The photodynamic treatment mediated by the alcohol-extracted sodium chlorophyllin from Spirulina showed excellent inhibitory effects on bacteria and tumor cells. This study initially reveals its excellent photodynamic performance and provides a reference for its in-depth application in the field of photodynamic therapy.展开更多
为及时了解国内外对光动力杀菌的研究现状、热点和发展趋势,为相关研究领域的研究者提供参考,本文采用文献计量学方法对Web of Science核心数据库中收录的2002~2022年光动力杀菌相关研究文献进行统计分析。同时,结合可视化分析软件Cites...为及时了解国内外对光动力杀菌的研究现状、热点和发展趋势,为相关研究领域的研究者提供参考,本文采用文献计量学方法对Web of Science核心数据库中收录的2002~2022年光动力杀菌相关研究文献进行统计分析。同时,结合可视化分析软件Citespace对不同国家和地区、机构、作者等进行分析,并对光动力杀菌研究的关键词进行聚类分析和突现分析。通过分析可知,在食品领域,光动力灭菌技术的研究方向主要包括光敏剂的选择和改造、光源的选择和优化以及延长食品货架期等;此外,光动力联合其他冷灭菌技术增强灭菌效果也将成为未来光动力在食品领域的一个研究趋势。展开更多
通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200...通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200 mW/cm2的溴钨灯光照射30 m in杀灭了99.9999%的单增李斯特菌,并导致了其蛋白质降解和基因组DNA片段断裂。血啉甲醚对单增李斯特菌的光动力灭活作用非常显著,其灭活机理可能是通过对蛋白质降解和基因组DNA损伤实现的。展开更多
基金supported by Grant of Russian Science Foundation No.17-15-01263.
文摘Here,we discuss an important problem in medicine as development of efctive strategies for brain drug delivery.This problem is related to the blood-brain barrier(BBB),which is a“customs”controlling the entrance of different molecules from blood into the brain protecting the normal function of central nervous system(CNS).We show three interfaces of anatomical side of BBB and two functional types of BBB一physical and transporter barriers.Although this protective mechanism is essential for health of CNS,it also creates a hindrance to the entry of drugs into the brain.The BBB was discovered over 100 years ago but till now,there is no efective methods for brain drug delivery.There ane more than 70 approaches for overcoming BBB incuding physical,chenical and biological techniques but all of these tools have limitation to be widely used in clinical practice due to invasi venes,challenge in performing,very costly or lim-itation of drug concentration.Photodynamic therapy(PDT)is usual clinical method of surgical navigation for the resection of brain tumor and anti-cancer therapy.Nowadays,the application of PDT is considered as a potential promising tool for brain drug delivery via opening of BBB.Here,we show the first sucoessful experimental results in this field discussing the adventures and disadv antages of PDT-related BBB disruption as well as altematives to overcome these limitations and possi ble mechanisms with new pathways for brain clearance via gly mphatic and lymphatic systems.
基金Supported by National Natural Science Foundation of China(32060228)Guangxi Natural Science Foundation(2017GXNSFAA198112+5 种基金2019GXNSFAA245077)Guangxi Graduate Education Innovation Project(GJY2018116YCSW2019214YCSW2020225)Undergraduate Innovation Training Program(202010601164202010601093)。
文摘[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and photodynamic antitumor effect on rat glioma C6 cells. [Methods]The absorption spectrum and fluorescence spectrum of sodium chlorophyllin were measured by an ultraviolet spectrophotometer and a fluorescence spectrophotometer;the plate count method was used to investigate the photodynamic sterilization efficiency of sodium chlorophyllin on Escherichia coli and Staphylococcus aureus;and the MTT method was used to determine the photodynamic antitumor effect of sodium chlorophyllin on rat glioma cell C6. [Results] The sterilization rates of sodium chlorophyllin with the 100 J/cm^2 photodynamic treatment were 98.96% 1.284 and 100% 0 on S. aureus and E. coli, respectively. The half maximal inhibitory concentration(IC50) of the sodium chlorophyllin photodynamic therapy with 5, 10 and 20 J/cm^2 on C6 were 69.9, 48.21 and 47.56 μg/ml respectively, and the dark toxicity was extremely low at 0 J/cm^2. [Conclusions]The photodynamic treatment mediated by the alcohol-extracted sodium chlorophyllin from Spirulina showed excellent inhibitory effects on bacteria and tumor cells. This study initially reveals its excellent photodynamic performance and provides a reference for its in-depth application in the field of photodynamic therapy.
文摘为及时了解国内外对光动力杀菌的研究现状、热点和发展趋势,为相关研究领域的研究者提供参考,本文采用文献计量学方法对Web of Science核心数据库中收录的2002~2022年光动力杀菌相关研究文献进行统计分析。同时,结合可视化分析软件Citespace对不同国家和地区、机构、作者等进行分析,并对光动力杀菌研究的关键词进行聚类分析和突现分析。通过分析可知,在食品领域,光动力灭菌技术的研究方向主要包括光敏剂的选择和改造、光源的选择和优化以及延长食品货架期等;此外,光动力联合其他冷灭菌技术增强灭菌效果也将成为未来光动力在食品领域的一个研究趋势。
文摘通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200 mW/cm2的溴钨灯光照射30 m in杀灭了99.9999%的单增李斯特菌,并导致了其蛋白质降解和基因组DNA片段断裂。血啉甲醚对单增李斯特菌的光动力灭活作用非常显著,其灭活机理可能是通过对蛋白质降解和基因组DNA损伤实现的。
