The main chemical components of 13 Saudi honey samples (composed of winter and summer honeys) were identified according to anti-ulcer and antioxidant activity by using phytochemical and chromatographic analyses. Phyto...The main chemical components of 13 Saudi honey samples (composed of winter and summer honeys) were identified according to anti-ulcer and antioxidant activity by using phytochemical and chromatographic analyses. Phytochemical screening of ethyl acetate and water extracts was used to detect the presence of carbohydrates, flavonoids, amino acids, and phenolic acids. High-performance liquid chromatography (HPLC) of sugar contents was used to detect the presence of galactose in all of the honey samples. The other detected sugars were sucrose, fructose, and arabinose. Fifteen amino acids were detected in all of the honey samples: Prolin is dominant in all of the honey samples. The citric acid and tartaric acids of winter and summer honey were also detected, in addition to faint traces of free oxalic acid. Both samples were tested for amino acids, phenolic compounds, and sugars. The most crucial result derived in this study is the effect of summer honey on ulcers. The anti-ulcer activities were also evaluated, showing that oral administration of the honey samples reduced the intensity of ulcer scores when compared to the control group. Similarly, there was a highly significant reduction in the values of the ulcer indices and areas in rats that received the same sample (P < 0.05 and P < 0.01) in comparison with those of the ulcer control rats. Finally, the antioxidant activity of the honey samples was evaluated, revealing a proportional relationship between the anti-oxidant and anti-ulcer activities. The results of this study could be reached that the effectiveness of honey as an anti-ulcer increase with the increasing its antioxidant activities.展开更多
Objective:The effectiveness of the slimy substance in snail to regenerate and repair damaged areas on it body/shell lead to this investigation.Methods:The anti-ulcer’property of snail slime extracted from phylur moll...Objective:The effectiveness of the slimy substance in snail to regenerate and repair damaged areas on it body/shell lead to this investigation.Methods:The anti-ulcer’property of snail slime extracted from phylur mollusca(tropical snail) from the giant African snail Archachatina marginata(Fam.Arionidae) was investi gated using histamine,stress and indomethacin-induced ulcers.The solubility profile of extract was investiga ted in different solvents and at different temperatures.Chemical analysis was carried out to determine the type of constituents present in the slim,while acute toxicity test was carried out to evaluate its profile of toxicity The effect of the snail slim on gastrointestinal motility was investigated in mice,while the guinea pig ileum wa used to study the effect of the extract on contraction produced by acetylcholine and histamine.The snail slim contained copious quantity of protein,with varying amounts of simple sugars,carbohydrates and fats.Th slime was not soluble in most common solvents and increases in temperature,did not appear to increase its sol ubility.Results:The result further indicated that although the snail slime exhibited significant(P【0.05) an ti -ulcer induced by stress and histamine,it was most potent against ulcer induced by indomethacin.The snai slime potently inhibited gastrointestinal movement in mice in a dose-dependent manner;however,it was not a ble to inhibit contraction induced by acetylcholine and histamine in guinea pig ileum.Conclusion:The snai mucin possesses potent antiulcer properties without any toxic effect.The mechanism responsible for the anti-ul cer property may not be postulated with certainty but cytoprotective and anti-spasmodic activities are most likel to be involved.展开更多
Pomegranate (Punica granatum L.) belongs to genera Punica and family Punicaceae. It is a herbal preparation that has been suggested as useful in the treatment of gastrointestinal disorders. However, to our knowledge, ...Pomegranate (Punica granatum L.) belongs to genera Punica and family Punicaceae. It is a herbal preparation that has been suggested as useful in the treatment of gastrointestinal disorders. However, to our knowledge, no study has been conducted to evaluate this therapeutic property. In the present study the antiulcerogenic effects of pomegranate peel methanol extract, was tested on male Wistar albino rats. Oral pretreatment with peel extracts (25, 50 and 100 mg/kg) for 15 days protected the gastric mucosa against the damage induced by indomethacin (50 mg/kg). The incidence of ulceration in the control group was 100%. The best results were found in a dosage of 50 mg/kg in sour summer cultivar which inhibited the peptic ulcerin comparison with indomethacin induced gastric ulcer group. Lowest ulcer index (5.4 ± 0.55), an apparent decrease in the infiltration of polymorphonuclear leukocytes and hemorrhage were observed after administration of sour summer extracts (50 mg/kg). In conclusion present study showed that pomegranate peel extract, especially sour summer, has curative potential as an antiulcer, possibly via its high antioxidant activity. These results from pomegranate peel extract can provide an extra income and may contribute about good nutritional values of this product.展开更多
OBJECTIVE： Carica papaya is an important fruit with its seeds used in the treatment of ulcer in Nigeria This study investigated the anti-ulcerogenic and antioxidant activities of aqueous extract of Carica papaya seed...OBJECTIVE： Carica papaya is an important fruit with its seeds used in the treatment of ulcer in Nigeria This study investigated the anti-ulcerogenic and antioxidant activities of aqueous extract of Carica papaya seed against indomethacin-induced peptic ulcer in male rats. METHODS： Thirty male rats were separated into 6 groups （A-F） of five rats each. For 14 d before ulcer induction with indomethacin, groups received once daily oral doses of vehicle （distilled water）, cimetidine 200 mg/kg body weight （BW）, or aqueous extract of C. papaya seed at doses of 100, 150 or 200 mg/kg BW （groups A, B, C, D, E and F, respectively）. Twenty-four hours after the last treatment, groups B, C, D, E and F were treated with 100 mg/kg BW of indomethacin to induce ulcer formation. RESULTS： Carica papaya seed extract significantly （P〈0.05） increased gastric pH and percentage of ulcer inhibition relative to indomethacin-induced ulcer rats. The extract significantly （P〈0.05） decreased gastric acidity, gastric acid output, gastric pepsin secretion, ulcer index and gastric secretion volume relative to group B. These results were similar to that achieved by pretreatment with cimetidine. Specific activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase in the extract-treated groups （D, E and F） were increased significantly over the group B （P〈0.05）. Pretreatment with the seed extract protected rats from the indomethacin-mediated decrease in enzyme function experienced by the group B. Similarly, indomethacin-mediated decrease in reduced glutathione level and indomethacin-mediated increase in malondialdehyde were reversed by Carica papaya extract. CONCLUSION： In this study, pretreatment with aqueous extract of Carica papaya seed exhibited anti- ulcerogenic and antioxidant effects, which may be due to the enhanced antioxidant enzymes.展开更多
文摘The main chemical components of 13 Saudi honey samples (composed of winter and summer honeys) were identified according to anti-ulcer and antioxidant activity by using phytochemical and chromatographic analyses. Phytochemical screening of ethyl acetate and water extracts was used to detect the presence of carbohydrates, flavonoids, amino acids, and phenolic acids. High-performance liquid chromatography (HPLC) of sugar contents was used to detect the presence of galactose in all of the honey samples. The other detected sugars were sucrose, fructose, and arabinose. Fifteen amino acids were detected in all of the honey samples: Prolin is dominant in all of the honey samples. The citric acid and tartaric acids of winter and summer honey were also detected, in addition to faint traces of free oxalic acid. Both samples were tested for amino acids, phenolic compounds, and sugars. The most crucial result derived in this study is the effect of summer honey on ulcers. The anti-ulcer activities were also evaluated, showing that oral administration of the honey samples reduced the intensity of ulcer scores when compared to the control group. Similarly, there was a highly significant reduction in the values of the ulcer indices and areas in rats that received the same sample (P < 0.05 and P < 0.01) in comparison with those of the ulcer control rats. Finally, the antioxidant activity of the honey samples was evaluated, revealing a proportional relationship between the anti-oxidant and anti-ulcer activities. The results of this study could be reached that the effectiveness of honey as an anti-ulcer increase with the increasing its antioxidant activities.
文摘Objective:The effectiveness of the slimy substance in snail to regenerate and repair damaged areas on it body/shell lead to this investigation.Methods:The anti-ulcer’property of snail slime extracted from phylur mollusca(tropical snail) from the giant African snail Archachatina marginata(Fam.Arionidae) was investi gated using histamine,stress and indomethacin-induced ulcers.The solubility profile of extract was investiga ted in different solvents and at different temperatures.Chemical analysis was carried out to determine the type of constituents present in the slim,while acute toxicity test was carried out to evaluate its profile of toxicity The effect of the snail slim on gastrointestinal motility was investigated in mice,while the guinea pig ileum wa used to study the effect of the extract on contraction produced by acetylcholine and histamine.The snail slim contained copious quantity of protein,with varying amounts of simple sugars,carbohydrates and fats.Th slime was not soluble in most common solvents and increases in temperature,did not appear to increase its sol ubility.Results:The result further indicated that although the snail slime exhibited significant(P【0.05) an ti -ulcer induced by stress and histamine,it was most potent against ulcer induced by indomethacin.The snai slime potently inhibited gastrointestinal movement in mice in a dose-dependent manner;however,it was not a ble to inhibit contraction induced by acetylcholine and histamine in guinea pig ileum.Conclusion:The snai mucin possesses potent antiulcer properties without any toxic effect.The mechanism responsible for the anti-ul cer property may not be postulated with certainty but cytoprotective and anti-spasmodic activities are most likel to be involved.
文摘Pomegranate (Punica granatum L.) belongs to genera Punica and family Punicaceae. It is a herbal preparation that has been suggested as useful in the treatment of gastrointestinal disorders. However, to our knowledge, no study has been conducted to evaluate this therapeutic property. In the present study the antiulcerogenic effects of pomegranate peel methanol extract, was tested on male Wistar albino rats. Oral pretreatment with peel extracts (25, 50 and 100 mg/kg) for 15 days protected the gastric mucosa against the damage induced by indomethacin (50 mg/kg). The incidence of ulceration in the control group was 100%. The best results were found in a dosage of 50 mg/kg in sour summer cultivar which inhibited the peptic ulcerin comparison with indomethacin induced gastric ulcer group. Lowest ulcer index (5.4 ± 0.55), an apparent decrease in the infiltration of polymorphonuclear leukocytes and hemorrhage were observed after administration of sour summer extracts (50 mg/kg). In conclusion present study showed that pomegranate peel extract, especially sour summer, has curative potential as an antiulcer, possibly via its high antioxidant activity. These results from pomegranate peel extract can provide an extra income and may contribute about good nutritional values of this product.
文摘OBJECTIVE： Carica papaya is an important fruit with its seeds used in the treatment of ulcer in Nigeria This study investigated the anti-ulcerogenic and antioxidant activities of aqueous extract of Carica papaya seed against indomethacin-induced peptic ulcer in male rats. METHODS： Thirty male rats were separated into 6 groups （A-F） of five rats each. For 14 d before ulcer induction with indomethacin, groups received once daily oral doses of vehicle （distilled water）, cimetidine 200 mg/kg body weight （BW）, or aqueous extract of C. papaya seed at doses of 100, 150 or 200 mg/kg BW （groups A, B, C, D, E and F, respectively）. Twenty-four hours after the last treatment, groups B, C, D, E and F were treated with 100 mg/kg BW of indomethacin to induce ulcer formation. RESULTS： Carica papaya seed extract significantly （P〈0.05） increased gastric pH and percentage of ulcer inhibition relative to indomethacin-induced ulcer rats. The extract significantly （P〈0.05） decreased gastric acidity, gastric acid output, gastric pepsin secretion, ulcer index and gastric secretion volume relative to group B. These results were similar to that achieved by pretreatment with cimetidine. Specific activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase in the extract-treated groups （D, E and F） were increased significantly over the group B （P〈0.05）. Pretreatment with the seed extract protected rats from the indomethacin-mediated decrease in enzyme function experienced by the group B. Similarly, indomethacin-mediated decrease in reduced glutathione level and indomethacin-mediated increase in malondialdehyde were reversed by Carica papaya extract. CONCLUSION： In this study, pretreatment with aqueous extract of Carica papaya seed exhibited anti- ulcerogenic and antioxidant effects, which may be due to the enhanced antioxidant enzymes.
