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Mobile genetic elements facilitate the transmission of antibiotic resistance genes in multidrug-resistant Enterobacteriaceae from duck farms
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作者 Xin’er Zheng Dingting Xu +5 位作者 Jinchang Yan Min Qian Peng Wang Davood Zaeim Jianzhong Han Daofeng Qu 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期729-735,共7页
Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms i... Multidrug-resistant(MDR)Enterobacteriaceae critically threaten duck farming and public health.The phenotypes,genotypes,and associated mobile genetic elements(MGEs)of MDR Enterobacteriaceae isolated from 6 duck farms in Zhejiang Province,China,were investigated.A total of 215 isolates were identified as Escherichia coli(64.65%),Klebsiella pneumoniae(12.09%),Proteus mirabilis(10.23%),Salmonella(8.84%),and Enterobacter cloacae(4.19%).Meanwhile,all isolates were resistant to at least two antibiotics.Most isolates carried tet(A)(85.12%),blaTEM(78.60%)and sul1(67.44%)resistance genes.Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons.A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004.This isolate was resistant to 18 antibiotics and carried the blaNDM-5 gene.MGEs,especially plasmids,are the primary antibiotic resistance gene transmission route in duck farms.These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety. 展开更多
关键词 Duck farm Mobile genetic element antibiotic resistance gene PLASMID Food safety
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Performance Parameters:Demobilization Antibiotic Resistant Bacteria(ARB)and Carrying Genes(ARG)in Wastewater Disinfection
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作者 Solange Kazue Utimura Denise Crocce Romano Espinosa +2 位作者 Marcio Luís Busi da Silva Elisabete de Santis Braga Pedro Jose Alvarez 《Journal of Environmental Science and Engineering(B)》 2024年第1期1-8,共8页
The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacteri... The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacterial control.The influence of some parameters in quantification and performance influencing of pathogen demobilization could be considered in disinfection of wastewater.The comparison of Polyvalent phage(NE1)versus Coliphage(NE4)in suppressing a bacterium Escherichia coli(NDM-1:b-lactam-resistant)with UV irradiation was observed the efficacy in reduction of cells in the disinfection and parameter process.The results with the effect of UV-C irradiation on NDM-1 infected with 1%of NE4 showed a decrease of cells from 8×10^(6)to 2×10^(5)in 60 min with UV-C dose.The NDM1(E.coli)was infected with 1%of NE4(Polyvalent Phage)under magnetic stirring for 1 h,the cells count was 8×10^(6).After 1 h in UV-C e×posure,the cells number reached 3×10^(5).The NDM1 that was e×posed in 1 h of UV-C irradiation and then was infected with 1%of NE4.Cells counting were done 24 h after this procedure.These cells were e×posed in UV-C and showed a reduction in the number of cells from 1×10^(8)to 4×10^(5)after 60 min.The results indicate that bacteriophages can mitigate bacteria species,and combined the conventional water disinfection technologies that can support the microbial safety control strategies. 展开更多
关键词 antibiotic Resistant Bacteria(ARB) antibiotic resistance genes(arg) wastewater treatment DISINFECTION Escherichia coli(E.coli).
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The Role of Wastewater Treatment Plants in the Environmental Dissemination of Antibiotic Resistant Bacteria (ARB) and Resistance Genes (ARG)
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作者 Abidelfatah M. Nasser Heitam Fawaqa Yeshayahu Nitzan 《Journal of Water Resource and Protection》 2019年第8期981-994,共14页
This study was conducted to evaluate the influence of wastewater treatment processes on the prevalence of antibiotic resistance fecal coliform (FC) and antibiotic resistance genes (ARGs) of FC. In addition, the occurr... This study was conducted to evaluate the influence of wastewater treatment processes on the prevalence of antibiotic resistance fecal coliform (FC) and antibiotic resistance genes (ARGs) of FC. In addition, the occurrence of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) in surface waters receiving wastewater was evaluated. Greater resistance against penicillin (P), colisitin (CT) and ampicillin (AMP) were observed for FC isolated from effluent disinfected by chlorine (71%), than that disinfected by UV (45%). The greatest resistance against six antibiotics was recorded for FC isolates from effluent disinfected by chlorine. The prevalence of tetB and blaSHV was lowest in isolates from chlorine-disinfected effluents. The occurrence of ARG blaSHV was highest in FC isolated from effluent disinfected by UV. A significant correlation was recorded between FC levels in surface waters and the level of bacterial resistance to ampicillin (P SHV in effluents and in surface waters. TetA and tetC were highly prevalent in surface water compared to tetB. The results of the study demonstrate the widespread prevalence of ARB and ARG in wastewater and receiving water bodies. The result indicates that the source of ARB and ARG in surface waters originate from wastewater. Released ARB and ARG may serve as the source of ARG to pathogenic bacteria in surface waters. Disinfection processes may influence the selection of antibiotic resistant patterns of bacteria. 展开更多
关键词 WASTEWATER antibiotic Resistant Bacteria genes Treatment DISINFECTION
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UV-Based Advanced Oxidation Processes for Antibiotic Resistance Control: Efficiency, Influencing Factors, and Energy Consumption
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作者 Jiarui Han Wanxin Li +5 位作者 Yun Yang Xuanwei Zhang Siyu Bao Xiangru Zhang Tong Zhang Kenneth Mei Yee Leung 《Engineering》 SCIE EI CAS CSCD 2024年第6期27-39,共13页
Antibiotic resistant bacteria(ARB)with antibiotic resistance genes(ARGs)can reduce or eliminate the effectiveness of antibiotics and thus threaten human health.The United Nations Environment Programme considers antibi... Antibiotic resistant bacteria(ARB)with antibiotic resistance genes(ARGs)can reduce or eliminate the effectiveness of antibiotics and thus threaten human health.The United Nations Environment Programme considers antibiotic resistance the first of six emerging issues of concern.Advanced oxidation processes(AOPs)that combine ultraviolet(UV)irradiation and chemical oxidation(primarily chlorine,hydrogen peroxide,and persulfate)have attracted increasing interest as advanced water and wastewater treatment technologies.These integrated technologies have been reported to significantly elevate the efficiencies of ARB inactivation and ARG degradation compared with direct UV irradiation or chemical oxidation alone due to the generation of multiple reactive species.In this study,the performance and underlying mechanisms of UV/chlorine,UV/hydrogen peroxide,and UV/persulfate processes for controlling ARB and ARGs were reviewed based on recent studies.Factors affecting the process-specific efficiency in controlling ARB and ARGs were discussed,including biotic factors,oxidant dose,UV fluence,pH,and water matrix properties.In addition,the cost-effectiveness of the UV-based AOPs was evaluated using the concept of electrical energy per order.The UV/chlorine process exhibited a higher efficiency with lower energy consumption than other UV-based AOPs in the wastewater matrix,indicating its potential for ARB inactivation and ARG degradation in wastewater treatment.Further studies are required to address the trade-off between toxic byproduct formation and the energy efficiency of the UV/chlorine process in real wastewater to facilitate its optimization and application in the control of ARB and ARGs. 展开更多
关键词 Advanced oxidation processes Ultraviolet/chlorine Ultraviolet/hydrogen peroxide Ultraviolet/persulfate antibiotic resistant bacteria antibiotic resistance genes
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Fate and Behavior of Tetracycline Resistance Genes in Activated Carbon Adsorption
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作者 Sri Anggreini Alma Rizky Aurellya +1 位作者 Wenqing Li Fusheng Li 《Journal of Water Resource and Protection》 CAS 2024年第1期1-16,共16页
The accessibility of tetracycline resistance gene (tetG) into the pores of activated carbon (AC), as well as the impact of the pore size distribution (PSD) of AC on the uptake capacity of tetG, were investigated using... The accessibility of tetracycline resistance gene (tetG) into the pores of activated carbon (AC), as well as the impact of the pore size distribution (PSD) of AC on the uptake capacity of tetG, were investigated using eight types of AC (four coal-based and four wood-based). AC showed the capability to admit tetG and the average reduction of tetG for coal-based and wood-based ACs at the AC dose of 1 g·L<sup>-1</sup> was 3.12 log and 3.65 log, respectively. The uptake kinetic analysis showed that the uptake of the gene followed the pseudo-second-order kinetics reaction, and the uptake rate constant for the coal-based and wood-based ACs was in the range of 5.97 × 10<sup>-12</sup> - 4.64 × 10<sup>-9</sup> and 7.02 × 10<sup>-11</sup> - 1.59 × 10<sup>-8</sup> copies·mg<sup>-1</sup>·min<sup>-1</sup>, respectively. The uptake capacity analysis by fitting the obtained experiment data with the Freundlich isotherm model indicated that the uptake constant (K<sub>F</sub>) values were 1.71 × 10<sup>3</sup> - 8.00 × 10<sup>9</sup> (copies·g<sup>-1</sup>)<sup>1-1/n</sup> for coal-based ACs and 7.00 × 10<sup>8</sup> - 3.00 × 10<sup>10</sup> (copies·g<sup>-1</sup>)<sup>1-1/n</sup> for wood-based ones. In addition, the correlation analysis between K<sub>F</sub> values and pore volume as well as pore surface at different pore size regions of ACs showed that relatively higher positive correlation was found for pores of 50 - 100 Å, suggesting ACs with more pores in this size region can uptake more tetG. The findings of this study are valuable as reference for optimizing the adsorption process regarding antibiotic resistance-related concerns in drinking water treatment. 展开更多
关键词 antibiotic resistance genes ADSORPTION Activated Carbon Drinking Water Treatment
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Dissemination of Resistance Integrons and Genes Coding for Blse and Cabapenemases in the Urban Drainage Network in Cote d’Ivoire
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作者 Coulibaly Kalpy Julien Diaby Aboubakar Sidik +8 位作者 Vakou N’dri Sabine M’bengue Gbonon Valérie Carole Claon Jean Stephane Yao Kouamé Eric Gnali Gbohounou Fabrice Yéo Yéfougnini Bagré Issa Djaman Allico Joseph Dosso Mireille 《Advances in Microbiology》 CAS 2024年第5期268-286,共19页
Antibiotic resistance has become a major threat to human health worldwide. Environment, particularly the water environment, has long been overlooked as a player in the antibiotic resistance cycle, although its role re... Antibiotic resistance has become a major threat to human health worldwide. Environment, particularly the water environment, has long been overlooked as a player in the antibiotic resistance cycle, although its role remains unclear. These can provide an ideal setting for the acquisition and dissemination of antibiotic resistance, as they are frequently affected by anthropogenic activities. The objective of this study was to establish a diffusion map of resistance integrons used as genetic markers of resistance associated with antibiotic resistance conferring genes (ARGs). Total DNA extracts from non-cultivable bacterial communities were used for the analyses. These communities were obtained from wastewater samples from 14 sites upstream and downstream of drainage channels or effluents in the cities of Abidjan, Bouaké, and Yamoussoukro. The results obtained correspond to the number of positives among the treated samples (n = 39). Among the genetic markers of dissemination, class 1 integrons were the most evident in 94.8% of samples in Abidjan (93.3%), Bouaké (100%) and Yamoussoukro (91.6%). Class 2 integrons and class 3 integrons were found respectively in 41% and 51% of all samples. Genes coding for β-lactamases and blaTEM was identified in almost all samples at a rate of 97.4%. A co-presence of the three genes blaTEM, blaSHV and blaCTX-M is also remarkable in the sites of the city of Yamoussoukro. Among the genes coding for carbapenemases, only blaKPC 17.94%, blaNDM 30.76% and blaOXA48 38.46% were detected in the samples. 展开更多
关键词 antibiotic resistance WASTEWATER resistance Integrons (RIs) resistance genes
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Analysis of antibiotic resistance genes and their effects on marine environment 被引量:1
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作者 DAI Yuanyuan GAO Yan DONG Yubo 《Marine Science Bulletin》 CAS 2020年第2期53-60,共8页
The application of overgrowth antibiotic resistance genes in marine research was discussed.Using the China National Knowledge Infrastructure(CNKI)network database to retrieve papers on resistance genes(n=30627)and ant... The application of overgrowth antibiotic resistance genes in marine research was discussed.Using the China National Knowledge Infrastructure(CNKI)network database to retrieve papers on resistance genes(n=30627)and antibiotic resistance genes(n=3277),the published trends,subject headings and other aspects of bibliometrics were analyzed.Literature in this field has increased rapidly in the past 38 years.55.3%of the papers were published in the last ten years,31.2%of the papers were published in the last five years.The relevance of research topics has increased in recent years,the current research mainly focus on antibiotics,resistance gene,antibiotic resistance gene,and drug resistance.Literature analysis is helpful to understand the overall development trend of overgrowth antibiotic resistance genes in marine research. 展开更多
关键词 CNKI database antibiotic resistance genes BIBLIOMETRICS
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Trifunctional Cu-Mesh/Cu_(2)O@FeO Nanoarrays for Highly Efficient Degradation of Antibiotic, Inactivation of Antibiotic-Resistant Bacteria, and Damage of Antibiotics Resistance Genes
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作者 Long Zhao Wei Zhou +6 位作者 Ming Wen Qingsheng Wu Weiying Li Yongqing Fu Quanjing Zhu Sheng Chen and Jiaqi Ran 《Energy & Environmental Materials》 SCIE EI CAS CSCD 2023年第1期349-359,共11页
Trifunctional Cu-mesh/Cu_(2)O@FeO nanoarrays heterostructure is designed and fabricated by integrating CuCu_(2)O@FeO nanoarrays onto Cu-mesh(CM)via an in situ growth and phase transformation process.It is successfully... Trifunctional Cu-mesh/Cu_(2)O@FeO nanoarrays heterostructure is designed and fabricated by integrating CuCu_(2)O@FeO nanoarrays onto Cu-mesh(CM)via an in situ growth and phase transformation process.It is successfully applied to efficiently mitigate the antibiotic pollution,including degradation of antibiotics,inactivation of antibiotic-resistant bacteria(ARB),and damage of antibiotics resistance genes(ARGs).Under visible-light irradiation,CM/CuCu_(2)O@FeO nanoarrays exhibit a superior degradation efficiency on antibiotics(e.g.,up to 99%in 25 min for tetracycline hydrochloride,TC),due to the generated reactive oxygen species(ROS),especially the dominant·O^(2−).It can fully inactivate E.coli(HB101)with initial number of~108 CFU mL^(−1) in 10 min,which is mainly attributed to the synergistic effects of 1D nanostructure,dissolved metal ions,and generated ROS.Meanwhile,it is able to damage ARGs after 180 min of photodegradation,including tetA(vs TC)of 3.3 log 10,aphA(vs kanamycin sulfate,KAN)of 3.4 log 10,and tnpA(vs ampicillin,AMP)of 4.4 log 10,respectively.This work explores a green way for treating antibiotic pollution under visible light. 展开更多
关键词 antibiotic antibiotic resistance genes antibiotic-resistant bacteria Cu-Mesh/Cu_(2)O@FeO nanoarrays photocatalytic degradation
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Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics 被引量:13
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作者 Bahareh Attaran Tahereh Falsafi Nassim Ghorbanmehr 《World Journal of Gastroenterology》 SCIE CAS 2017年第7期1163-1170,共8页
To evaluate the role of biofilm formation on the resistance of Helicobacter pylori (H. pylori) to commonly prescribed antibiotics, the expression rates of resistance genes in biofilm-forming and planktonic cells were ... To evaluate the role of biofilm formation on the resistance of Helicobacter pylori (H. pylori) to commonly prescribed antibiotics, the expression rates of resistance genes in biofilm-forming and planktonic cells were compared.METHODSA collection of 33 H. pylori isolates from children and adult patients with chronic infection were taken for the present study. The isolates were screened for biofilm formation ability, as well as for polymerase chain reaction (PCR) reaction with HP1165 and hp1165 efflux pump genes. Susceptibilities of the selected strains to antibiotic and differences between susceptibilities of planktonic and biofilm-forming cell populations were determined. Quantitative real-time PCR (qPCR) analysis was performed using 16S rRNA gene as a H. pylori-specific primer, and two efflux pumps-specific primers, hp1165 and hefA.RESULTSThe strains were resistant to amoxicillin, metronidazole, and erythromycin, except for one strain, but they were all susceptible to tetracycline. Minimum bactericidal concentrations of antibiotics in the biofilm-forming cells were significantly higher than those of planktonic cells. qPCR demonstrated that the expression of efflux pump genes was significantly higher in the biofilm-forming cells as compared to the planktonic ones.CONCLUSIONThe present work demonstrated an association between H. pylori biofilm formation and decreased susceptibility to all the antibiotics tested. This decreased susceptibility to antibiotics was associated with enhanced functional activity of two efflux pumps: hp1165 and hefA. 展开更多
关键词 Helicobacter pylori BIOFILM antibiotic resistance Efflux genes hp1165 hefA
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Genetic Diversity,Antibiotic Resistance,and Pathogenicity of Aeromonas Species from Food Products in Shanghai,China 被引量:1
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作者 QU Feng Tian WANG Wen Qing +8 位作者 LIU Qian ZHOU Hai Jian HU Jin Rui DU Xiao Li WANG Yue XUE Jia Qi CUI Zhi Gang XIE Gui Lin MENG Shuang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2022年第9期842-853,共12页
Objective Aeromonas has recently been recognized as an emerging human pathogen.Aeromonasassociated diarrhea is a phenomenon occurring worldwide.This study was designed to determine the prevalence,genetic diversity,ant... Objective Aeromonas has recently been recognized as an emerging human pathogen.Aeromonasassociated diarrhea is a phenomenon occurring worldwide.This study was designed to determine the prevalence,genetic diversity,antibiotic resistance,and pathogenicity of Aeromonas strains isolated from food products in Shanghai.Methods Aeromonas isolates(n=79)collected from food samples were analyzed using concatenated gyrB-cpn60 sequencing.The antibiotic resistance of these isolates was determined using antimicrobial susceptibility testing.Pathogenicity was assessed usingβ-hemolytic,extracellular protease,virulence gene detection,C.elegans liquid toxicity(LT),and cytotoxicity assays.Results Eight different species were identified among the 79 isolates.The most prevalent Aeromonas species were A.veronii[62(78.5%)],A.caviae[6(7.6%)],A.dhakensis[3(3.8%)],and A.salmonicida[3(3.8%)].The Aeromonas isolates were divided into 73 sequence types(STs),of which 65 were novel.The isolates were hemolytic(45.6%)and protease-positive(81.0%).The most prevalent virulence genes were act(73.4%),fla(69.6%),aexT(36.7%),and ascV(30.4%).The results of C.elegans LT and cytotoxicity assays revealed that A.dhakensis and A.hydrophila were more virulent than A.veronii,A.caviae,and A.bivalvium.Antibiotic resistance genes[tetE,blaTEM,tetA,qnrS,aac(6)-Ib,mcr-1,and mcr-3]were detected in the isolates.The multidrug-resistance rate of the Aeromonas isolates was 11.4%,and 93.7%of the Aeromonas isolates were resistant to cefazolin.Conclusion The taxonomy,antibiotic resistance,and pathogenicity of different Aeromonas species varied.The Aeromonas isolates A.dhakensis and A.hydrophila were highly pathogenic,indicating that food-derived Aeromonas isolates are potential risks for public health and food safety.The monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China. 展开更多
关键词 AEROMONAS Genetic diversity antibiotic resistance Virulence gene Cytotoxicity assay MULTIDRUG-resistance
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Identification and characterization of integron mediated antibiotic resistance in pentachlorophenol degrading bacterium isolated from the chemostat
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作者 SHARMA Ashwani THAKUR Indu Shekhar 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第6期858-864,共7页
A bacterial consortium was developed by continuous enrichment of microbial population isolated from sediment core of pulp and paper mill effluent in mineral salts medium(MSM) supplemented with pentachlorophenol(PCP... A bacterial consortium was developed by continuous enrichment of microbial population isolated from sediment core of pulp and paper mill effluent in mineral salts medium(MSM) supplemented with pentachlorophenol(PCP) as sole source of carbon and energy in the chemostat.The consortia contained three bacterial strains.They were identified as Escherichia coli,Pseudomonas aeruginosa and Acinetobacter sp.by 16S rRNA gene sequence analysis.Acinetobacter sp.readily degraded PCP through the formation of tetrachloro-p-hydroquinone(TecH),2-chloro-1,4-benzenediol and products of ortho ring cleavage detected by gas chromatograph/mass spectrometer(GC-MS).Out of the three acclimated PCP degrading bacterial strains only one strain,Acinetobacter sp.showed the presence of integron gene cassette as a marker of its stability and antibiotic resistance.The strain possessed a 4.17 kb amplicon with 22 ORF's.The plasmid isolated from the Acinetobacter sp.was subjected to shotgun cloning through restriction digestion by BamHI,HindIII and SalI,ligated to pUC19 vector and transformed into E.coli XLBlue1α,and finally selected on MSM containing PCP as sole source of carbon and energy with ampicillin as antibiotic marker.DNA sequence analysis of recombinant clones indicated homology with integron gene cassette and multiple antibiotic resistance genes. 展开更多
关键词 Acinetobacter sp. antibiotic resistance gene CHEMOSTAT integron gene cassette PENTACHLOROPHENOL recombinant clone
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Streptococcus peumoniae in an Egyptian urban community:incidence of erythromycin-resistance determinants and antibiotic susceptibility profile
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作者 Fatma Abdelaziz Amer Eman Mohamed Elbehedy Mohamed Elahmady 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2008年第4期20-23,共4页
Objectives:To determine the incidence of resistance of Streptococcus(Strep).pneumoniae isolated in our locality to erythromycin,to screen for the two resistance determinants erm(B) and mef(A) genes,and to identify the... Objectives:To determine the incidence of resistance of Streptococcus(Strep).pneumoniae isolated in our locality to erythromycin,to screen for the two resistance determinants erm(B) and mef(A) genes,and to identify the susceptibility profile to commonly used antibiotics.Methods:Samples were collected from patients attending the Outpatient Department of Zagazig University Hospital,Zagazig,Egypt,between February 2006 and March 2007.Strep.pneumoniae was identified by conventional procedures.Susceptibilities to erythromycin and 15 antibiotics were identified by disc diffusion method,as outlined by CLSI.E-test was used for MIC determination of erythromycin.erm(B) and mef(A) genes were detected by PCR.Results:Eighty-one Strep. pneumoniae strains were identified.Fifty- one of them(63%) were erythromycin-resistant,and mef(A) gene was the predominant resistance determinant.Vancomycin,imipenem and gatifloxacin had the best activity against the isolates,whereas tetracycline had the least.Forty-two(51.85%) out of the 81 Strep.pneumoniae strains were multidrug-resistant.Conclusions:High incidence of resistance to erythromycin and multiple antimicrobials existed.mef(A) was the principal erythromycin-resistance gene. 展开更多
关键词 Strep pneumoniae ERYTHROMYCIN erm.(B) GENE mef(A) GENE antibiotic resistance Egypt
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Extended role for insertion sequence elements in the antibiotic resistance of Bacteroides 被引量:1
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作者 József Sóki 《World Journal of Clinical Infectious Diseases》 2013年第1期1-12,共12页
The Bacteroides species are important micro-organisms, both in the normal physiology of the intestines and as frequent opportunistic anaerobic pathogens, with a deeply-rooted phylogenetic origin endowing them with som... The Bacteroides species are important micro-organisms, both in the normal physiology of the intestines and as frequent opportunistic anaerobic pathogens, with a deeply-rooted phylogenetic origin endowing them with some interesting biological features. Their prevalence in anaerobic clinical specimens is around 60%-80%, and they display the most numerous and highest rates of antibiotic resistance among all pathogenic anaerobes. In these antibiotic resistance mechanisms there is a noteworthy role for the insertion sequence(IS) elements, which are usually regarded as representatives of ‘selfish' genes; the IS elements of Bacteroides are usually capable of up-regulating the antibiotic resistance genes. These include the cep A(penicillin and cephalosporin), cfx A(cephamycin), cfi A(carbapenem), nim(metronidazole) and erm F(clindamycin) resistance genes. This is achieved by outwardoriented promoter sequences on the ISs. Although some representatives are well characterized, e.g., the resistance gene-IS element pairs in certain resistant strains, open questions remain in this field concerning a better understanding of the molecular biology of theantibiotic resistance mechanisms of Bacteroides, which will have clinical implications. 展开更多
关键词 antibiotic resistance antibiotic resistance genes Bacteroides fragilis Insertion sequence elements
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环境中抗生素抗性基因(ARGs)形成和传播机制研究进展 被引量:1
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作者 唐涛涛 赵晓龙 +3 位作者 王胤 杨舒茗 吴嘉利 朱榕鑫 《应用化工》 CAS CSCD 北大核心 2024年第8期1980-1984,1988,共6页
从DNA损伤和错误修复角度阐述了环境中抗生素抗性基因(Antibiotics resistance genes,ARGs)的形成机制,并对DNA损伤和错误修复过程中涉及的相关核心功能基因片段进行归纳总结。也从细胞代谢角度阐述了环境中ARGs进行水平传播的三种主要... 从DNA损伤和错误修复角度阐述了环境中抗生素抗性基因(Antibiotics resistance genes,ARGs)的形成机制,并对DNA损伤和错误修复过程中涉及的相关核心功能基因片段进行归纳总结。也从细胞代谢角度阐述了环境中ARGs进行水平传播的三种主要机制,包括:菌毛架桥作用、信号传导和IV型细胞分泌系统。现有研究进一步加强对ARGs在环境中形成和传播机制的认识,为今后研究ARGs的削减提供理论依据。目前关于抗生素诱导ARGs形成和传播的内在机制仍相对缺乏,后续可从生物毒性和分子生物学层面进行深入研究。 展开更多
关键词 抗生素抗性基因(args) 功能基因 水平传播 分子生物学
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微藻生物系统去除抗生素和ARGs的研究进展
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作者 赵学宇 任淑静 +3 位作者 郭宁 张欢欢 贾玉莹 朱兆亮 《净水技术》 CAS 2024年第4期28-33,45,共7页
抗生素与抗生素抗性基因(ARGs)污染严重危害人类健康,微藻生物技术在处理抗生素废水过程中表现出良好的效果。文章首先总结了微藻对于不同种类抗生素的去除机理,包括生物吸附、生物累积、生物降解、光生物降解和水解挥发5种。分析了微... 抗生素与抗生素抗性基因(ARGs)污染严重危害人类健康,微藻生物技术在处理抗生素废水过程中表现出良好的效果。文章首先总结了微藻对于不同种类抗生素的去除机理,包括生物吸附、生物累积、生物降解、光生物降解和水解挥发5种。分析了微藻在去除抗生素过程中的重要影响因素,包括pH、水力停留时间、光照、抗生素的种类与浓度和微藻的种类。并总结了微藻对ARGs的去除机制,包括移动基因元件的去除及活性氧自由基的猝灭。最后,文章指出了微藻处理抗生素废水过程中仍然存在的问题,进一步明确了今后微藻生物技术处理抗生素废水的重点研究方向。 展开更多
关键词 抗生素废水 微藻 抗生素抗性基因(args) 去除机理 影响因素
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Antibiotic resistance analysis and coping strategies of helicobacter pylori
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作者 CHEN Run-xiang ZHANG Xiao-dong +1 位作者 CHEN Shi-ju BAI Fei-hu 《Journal of Hainan Medical University》 CAS 2023年第20期72-78,共7页
Helicobacter pylori(H.pylori)is a Gram-negative bacterium that mainly colonizes the stomach and duodenum,and it can cause gastrointestinal diseases such as gastric inflammation,peptic ulcer and gastric cancer,and erad... Helicobacter pylori(H.pylori)is a Gram-negative bacterium that mainly colonizes the stomach and duodenum,and it can cause gastrointestinal diseases such as gastric inflammation,peptic ulcer and gastric cancer,and eradication of H.pylori can effectively stop the occurrence and development of gastrointestinal diseases.Antibiotics are one of the main drugs used to treat H.pylori.Due to the long-term application of antibiotics,the resistance rate of H.pylori to antibiotics increases year by year,which greatly reduces the eradication rate of H.pylori and increases the difficulty of re-treatment and the economic burden of patients.In this paper,we will review three aspects of H.pylori resistance status,resistance mechanism and treatment to provide reference for the progress of H.pylori resistance research and its treatment strategy. 展开更多
关键词 Helicobacter pylori antibiotic resistance Resistant gene TREATMENT
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Frequency of Antibiotic Resistance of <i>Escherichia coli</i>and <i>Klebsiella pneumoniae</i>by Production of TOHO-Type <i>β</i>-Lactamases at Saint Camille Hospital, Ouagadougou (Burkina Faso)
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作者 Serge Sougué Amana Mètuor-Dabiré +6 位作者 Djénéba Ouermi Yasmine Rahimatou Wend-Kouni Tiemtoré Bénao Stéphanie Lynseh Carine Sita Théodora Mahoukèdè Zohoncon Yasmine Aminata Bangré Elie Kabré Jacques Simporé 《Advances in Microbiology》 2021年第12期713-722,共10页
Extended-spectrum <i>β</i>-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on ... Extended-spectrum <i>β</i>-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEM, blaCTX-M and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-M subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value < 0.05 was considered as significant. We obtained at all 39 strains constituted by 21 (53.8%) <i>Escherichia coli</i> and 18 (46.2%) <i>Klebsiella pneumoniae</i>. Molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i>. 展开更多
关键词 antibiotic resistance Bacteria ESBL genes TOHO
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沈阳典型粳稻种植区稻田土壤ARGs和MGEs污染分布特征分析
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作者 张珣 黎馨月 高雨竹 《沈阳农业大学学报》 CAS CSCD 北大核心 2024年第3期306-315,共10页
农田土壤中抗生素抗性基因(ARGs)与可移动遗传元件(MGEs)的污染问题,在学术界已引发广泛关注。为研究沈阳市粳稻种植区稻田土壤中ARGs与MGEs的存在和相关性,并探讨其对土壤-植物系统的潜在影响,通过采集沈阳市辽中区、新民市、沈北新区... 农田土壤中抗生素抗性基因(ARGs)与可移动遗传元件(MGEs)的污染问题,在学术界已引发广泛关注。为研究沈阳市粳稻种植区稻田土壤中ARGs与MGEs的存在和相关性,并探讨其对土壤-植物系统的潜在影响,通过采集沈阳市辽中区、新民市、沈北新区和苏家屯区的粳稻种植区域土壤样品,利用高通量qPCR技术,对285个ARGs、10个MG-Es和一个16S rRNA基因进行检测和定量分析。结果表明:在不同地区的稻田土壤中共检测到273种ARGs和MGEs,且种类和丰度存在显著差异。进一步的相关性分析发现,ARGs与MGEs之间存在一定相关性,并且这种相关性在不同地区间有所差异。多种ARGs与MGEs之间存在显著正相关(p<0.05),其中部分ARGs[tet(32)等]与MGEs(tnpA-01等)存在及极显著正相关(p<0.01),这表明在土壤中MGEs对ARGs迁移和扩散起到促进作用。4个采样区根际土中ARGs和MGEs的总相对丰度均高于非根际土壤,这进一步凸显根际环境在ARGs和MGEs分布中的重要性。此外,还发现在不同地区的稻田土壤中,抗生素失活机制在ARGs中占据主导地位。综上所述,试验结果为深入了解东北水稻种植区稻田土壤中ARGs的污染现状及其潜在影响提供了数据支持和理论基础。这些发现不仅有助于认识农田土壤中ARGs和MGEs的分布与迁移规律,还为制定针对性的土壤污染防控措施提供了科学依据。 展开更多
关键词 粳稻稻田土壤 抗生素 抗生素抗性基因 可移动遗传元件
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除草剂对质粒介导ARGs接合转移的影响及机制
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作者 孙凤飞 王秀 +2 位作者 毛雨梦 刘珂 刘娟 《中国环境科学》 EI CAS CSCD 北大核心 2024年第11期6426-6434,共9页
本研究以同时携带四环素、氨苄青霉素和卡那霉素3种抗生素抗性基因(ARGs)的可移动质粒RP4为供体质粒(保存于供体菌E.coli DH5α中),以E.coli HB101为受体菌,以丁草胺、百草敌和草铵膦作为除草剂代表,研究了不同浓度除草剂对可移动质粒... 本研究以同时携带四环素、氨苄青霉素和卡那霉素3种抗生素抗性基因(ARGs)的可移动质粒RP4为供体质粒(保存于供体菌E.coli DH5α中),以E.coli HB101为受体菌,以丁草胺、百草敌和草铵膦作为除草剂代表,研究了不同浓度除草剂对可移动质粒介导的ARGs接合转移的影响,并从细菌细胞膜通透性、胞内ROS含量以及与接合转移相关调控基因的转录水平等角度,阐明了其作用机制.结果表明,100~800mg/L的丁草胺以及40~160mg/L的百草敌都可促进质粒RP4的接合转移.供试浓度范围内,丁草胺皆可刺激细菌胞内产生ROS,百草敌则使ROS水平下降.丁草胺和百草敌处理下,受体菌表面皆出现明显的皱缩,且随着浓度升高,出现孔洞甚至断裂现象.400~800mg/L的丁草胺和40~80mg/L的百草敌皆可通过提高细胞膜通透性,下调kor A基因的转录,同时上调trb Bp、trf Ap和tra A基因的转录,进而促进RP4的接合转移.供试浓度下,草铵膦对RP4的接合影响甚微,对细菌细胞膜通透性、胞内ROS水平以及接合相关调控基因转录水平的影响也不大.研究结果提示需要关注农田环境中除草剂的使用对ARGs传播的风险,并建议通过控制除草剂的种类及施用浓度来控制ARGs在农田生态系统中的接合转移. 展开更多
关键词 除草剂 可移动质粒RP4 抗生素抗性基因 水平迁移 二亲接合
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抗生素抗性基因(ARGs)--一种新型环境污染物 被引量:156
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作者 罗义 周启星 《环境科学学报》 CAS CSCD 北大核心 2008年第8期1499-1505,共7页
抗生素的环境污染与生态毒性近年来引起了日益广泛的关注.水产养殖和畜牧业抗生素长期滥用的直接后果,很可能诱导动物体内抗生素抗性基因,经排泄后将对养殖区域及其周边环境造成潜在基因污染.抗性基因还极有可能在环境中传播、扩散,对... 抗生素的环境污染与生态毒性近年来引起了日益广泛的关注.水产养殖和畜牧业抗生素长期滥用的直接后果,很可能诱导动物体内抗生素抗性基因,经排泄后将对养殖区域及其周边环境造成潜在基因污染.抗性基因还极有可能在环境中传播、扩散,对公共健康和食品、饮用水安全构成威胁.为此,提出了将抗生素抗性基因作为一类新型环境污染物,对该类污染物在环境中的来源、潜在的传播途径以及国内外相关研究进展进行综述,指出了当前形势下我国开展环境中抗生素抗性基因污染研究的必要性,建议尽快从国家层面上系统进行抗生素抗性基因的环境污染机理与控制对策研究. 展开更多
关键词 抗生素抗性基因 抗生素 水产养殖业 畜牧业 新型环境污染物
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