Objective To investigate the prevalence of antiendothelial cell antib odies and its possible role in the pathogenesis in lupus nephritis Methods Sera from 58 patients with lupus nephritis were studied Cellu l ar enzy...Objective To investigate the prevalence of antiendothelial cell antib odies and its possible role in the pathogenesis in lupus nephritis Methods Sera from 58 patients with lupus nephritis were studied Cellu l ar enzyme linked immunoabsaborbent assay was used to detect antiendothelia l cell a ntibodies, and immunoblotting technique was performed to determine its specific endothelial target antigens Results 362% of the patients with lupus nephritis had elevated IgG is o type antiendothelial cell antibodies The association was revealed between th e presence of antiendothelial cell antibodies (AECA) and some laboratory and re nal biopsy features, such as thrombocytopenia, higher serum IgG level, lower ser um C3 level, higher erythrocyte sedimentation rate (ESR), presence of antin uc lear antibody (ANA), antidsDNA antibodies and glomerular thrombosis Antien d othelial cell antibodies were consisted of a group of heterogeneous antibodies, and reacted with different endothelial cell antigens which ranged from 12-90?kD in molecular size in immunoblot A correlation was demonstrated between heavy p roteinuria (>35?g/24?hr) and the existence of antibodies against both 27 and 29 ?kD endothelial cell antigens, an association between the presence of glomerula r capillary thrombi and antibody against the 29?kD endothelial cell antigen was also found Conclusions Lupus nephritis patients with antiendothelial cell antibo dies showed a complex autoimmune disorder and clinical disease activity, and the results also provided an indirect evidence that antiendothelial cell antibodi es might contribute to the pathogenesis of lupus nephritis展开更多
文摘Objective To investigate the prevalence of antiendothelial cell antib odies and its possible role in the pathogenesis in lupus nephritis Methods Sera from 58 patients with lupus nephritis were studied Cellu l ar enzyme linked immunoabsaborbent assay was used to detect antiendothelia l cell a ntibodies, and immunoblotting technique was performed to determine its specific endothelial target antigens Results 362% of the patients with lupus nephritis had elevated IgG is o type antiendothelial cell antibodies The association was revealed between th e presence of antiendothelial cell antibodies (AECA) and some laboratory and re nal biopsy features, such as thrombocytopenia, higher serum IgG level, lower ser um C3 level, higher erythrocyte sedimentation rate (ESR), presence of antin uc lear antibody (ANA), antidsDNA antibodies and glomerular thrombosis Antien d othelial cell antibodies were consisted of a group of heterogeneous antibodies, and reacted with different endothelial cell antigens which ranged from 12-90?kD in molecular size in immunoblot A correlation was demonstrated between heavy p roteinuria (>35?g/24?hr) and the existence of antibodies against both 27 and 29 ?kD endothelial cell antigens, an association between the presence of glomerula r capillary thrombi and antibody against the 29?kD endothelial cell antigen was also found Conclusions Lupus nephritis patients with antiendothelial cell antibo dies showed a complex autoimmune disorder and clinical disease activity, and the results also provided an indirect evidence that antiendothelial cell antibodi es might contribute to the pathogenesis of lupus nephritis
