Phytochemical Analysis and Antimicrobial Activity of Lawsonia inermis Leaf Extracts from Burkina Faso

Lawsonia inermis is a hairless plant growing in various regions of North Africa, the Indian subcontinent, and the Middle East. It possesses many medicinal attributes, including curative properties against infectious dermatoses. This study was carried out to evaluate the phytochemical profile of the crude ethanolic extract of the plant leaves and its fractions as well as their antimicrobial activities. The phytochemical profile was performed using high-performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), and high-performance liquid chromatography (HPLC). Additionally, the phenolic and flavonoid contents were determined using the Folin-Ciocalteu spectrophotometric and the aluminum trichloride methods. Antimicrobial activity was tested using disc diffusion and microdilution methods. The presence of flavonoids, tannins, sterols, and triterpenes was revealed. GC-MS detected twelve compounds main compounds consisting of saturated and unsaturated fatty acids and phenolic and terpenoid compounds among twenty-seven components. HPLC also detected high contents of phenolic acids and flavonoids. The most abundant triterpene and sterols were ursolic acid (around 43.14 g/100g DW, 13.9 g/100g dry weight (DW), and 0.68 g/100g DW) in the crude ethanolic extract of leaves (FeLi), hexane fraction (FHLi) and dichloromethane fraction (FDLi), respectively and, β-sitosterol in FeLi (56.7 mg/100g DW), FHLi (10.55 g/100g DW), FDLi (106.1 mg/100g DW) and butanol fraction (FBLi) (357.4 mg/100g DW). Among the flavonoids, rutin = 3.24 g/100g and quercetin = 0.63 g/100g in the ethanolic extract, rutin = 15.73 g/100g in the dichloromethane fraction, and rutin = 0.23 g/100g) in the aqueous fraction;and among phenolic compounds, caffeic acid (37.65 g/100g DW) and vanillic acid (22.70 g/100g DW) were the most important in the ethyl acetate fraction (FAeLi). All organic fractions exhibited interesting antibacterial and antifungal activities against the tested strains, with the best activity recorded with the dichloromethane and ethyl acetate fractions. The leaf extracts’ phytochemical profile and antimicrobial activity support the use of Lawsonia inermis against infectious skin diseases.

Synthesis, Structural Characterization and Antimicrobial Activity of a Novel Cobalt(II) Complex Based on 3-Methyl-1-Phenyl-4-(2-Thienoyl)-Pyrazol-5-One

New cobalt(II) complex, [Co(O2C15H11N2S)2(OH2)2]∙2H2O (1∙2H2O), has been synthesized upon reaction of cobalt chloride hexahydrate (Co(Cl)2∙6H2O) with 3-methyl-1-Phenyl-4-(2-thienoyl)-pyrazol-5-one (referred as HL) in ethanol at room temperature. Single crystal X-ray diffraction (XRD), spectroscopic methods, and microelemental analyses were used to characterize 1∙2H2O. Compound 1∙2H2O crystallizes in the orthorhombic crystal system with a Pbca space group and with the cobalt atom being pseudo-octahedral coordinated. The broth microdilution technique was used to screen the free ligand (HL) and the complex (1∙2H2O) for antimicrobial activities. HL has a low activity (MIC > 100 μg/mL) on all microorganisms, whereas compound 1∙2H2O displayed moderate activity (10 ∙2H2O exhibited bactericidal and fungicidal activity respectively on all the bacteria and yeasts tested. These findings reveal that the antimicrobial activity of HL was enhanced upon coordination to Co(II) ion against all microorganisms (bacteria and fungus).

Synthesis, Structural Characterization and Antimicrobial Activity of a Novel Cobalt(II) Complex Based on 3-Methyl-1-Phenyl-4-(2-Thienoyl)-Pyrazol-5-One

New cobalt(II) complex, [Co(O2C15H11N2S)2(OH2)2]∙2H2O (1∙2H2O), has been synthesized upon reaction of cobalt chloride hexahydrate (Co(Cl)2∙6H2O) with 3-methyl-1-Phenyl-4-(2-thienoyl)-pyrazol-5-one (referred as HL) in ethanol at room temperature. Single crystal X-ray diffraction (XRD), spectroscopic methods, and microelemental analyses were used to characterize 1∙2H2O. Compound 1∙2H2O crystallizes in the orthorhombic crystal system with a Pbca space group and with the cobalt atom being pseudo-octahedral coordinated. The broth microdilution technique was used to screen the free ligand (HL) and the complex (1∙2H2O) for antimicrobial activities. HL has a low activity (MIC > 100 μg/mL) on all microorganisms, whereas compound 1∙2H2O displayed moderate activity (10 ∙2H2O exhibited bactericidal and fungicidal activity respectively on all the bacteria and yeasts tested. These findings reveal that the antimicrobial activity of HL was enhanced upon coordination to Co(II) ion against all microorganisms (bacteria and fungus).

A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia

To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,Russia.Totally,635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent approaches.The predominant genus was Streptomyces(74.5%),followed by Microbacterium(4.9%),Agromyces(3.5%),Nocardiopsis(3.0%),and Kitasatospora(2.5%).Seven strains showed relatively low 16 S rRNA similarities(<98.65%)with validly described species.Seventy-seven strains were selected as representatives to evaluate their antimicrobial activities and underlying mechanisms by paper-disk diffusion method and a double fluorescent protein reporter system(pDualrep 2),respectively.Streptomyces sp.S 6 b 3-1 was highlighted due to its strong inhibitory activities against Gram-positive bacteria and underlying antibacterial mechanism by inducing the SOS response in the reporter system.LG-1,(R)-6-[1-(benzoyloxy)ethyl]-phenazine-1-carboxylic acid,an analogue of saphenamycin was finally isolated from the culture broth of Streptomyces strain.This study preliminarily explored the actinobacterial diversity of soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time,which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia.

Composition and antimicrobial activity of Cistus munbyi essential oil: an endemic plant from Algeria

The chemical composition and antimicrobial potential of Cistus munbyi essential oil were studied for the first time. GC and GC–MS analysis revealed 48 compounds representing 96.98% of the oil. Terpinen-4-ol(23.75%), meta-Cymene(17.30%), and Sabinene(12.38%)were the major constituents. Antimicrobial activity was evaluated against nine pathogens using the disc diffusion and broth micro-dilution methods. Results show that C.munbyi essential oil possesses strong antimicrobial activity against all strains, regardless if Gram-positive or Gramnegative bacteria, or yeast, with MICs values not exceeding10(mg/ml). In addition to its efficacy, C. munbyi essential oil has an unusual antimicrobial potency which is attributed to its specific chemical composition. Thus, findings presented here suggest that endemic C. munbyi contains a very interesting essential oil that may be valuable in several areas.

DCC/DMAP mediated esterification of hydroxy and non-hydroxy olefinic fatty acids with β-sitosterol:In vitro antimicrobial activity

A new series of fatty alkenoates were synthesized using an appropriate synthetic route involving DCC and DMAP as catalysts. Compounds were characterized by their spectral data.All the synthesized compounds were evaluated for their in vitro antimicrobial activity.The minimum inhibitory concentration(MIC),minimum bacterial concentration(MBC) and minimum fungicidal concentration(MFC) were determined for test compounds as well as for reference standards.Among the compounds tested, compounds having hydroxy group at the fatty acid chain showed the most potent antibacterial as well as antifungal activities.

Studies on the Antimicrobial Activity of Chitooligosaccharides from Housefly Larvae, Musca domestica vicina Macquart(Diptera: Muscidae)

The inhibition effect of chitooligosaccharides from housefly larvae on pathogens ofcrops seeds, fruits or vegetables was studied and the main factors influencing theantimicrobial activity of chitooligosaccharides were also investigated. It was foundthat chitooligosaccharides from housefly larvae had wide spectrum fungistasis. It canstrongly inhibit the development of 31 kinds of plant pathogenic fungi such as corticiumrolfsii Saccardo. The results indicated that the degree of deacelylation (DD) or theaverage molecular weight(MW)of chitooligosaccharides were related to the antimicrobialactivity. The antimicrobial activity increased with the rising of DD or the declining ofMW. Pot culture results showed that chitooligosaccharide could enhance the rate ofgermination and emergence of the seeds of maize, wheat and cotton. Chitooligosaccharidehad certain effect on corn southern leaf blight caused by Helminthosporium maydis.

Antimicrobial Activity of Submerged Culture Extracts from Xylaria striata

[ Objective ] The paper was to explore the antimicmbial activities of extracts from Xylaria striata via submerged culture. [ Methods ] The antibacterial activities of X. striata extracts against 10 kinds of bacteria were determined using Oxford cup method and mJcroplate assay, and the antifungal activities against nine kinds of pathogenic fungi were determined using mycelial growth rate method. [ Results ] The inhibition zone diameters of the mycelium extract against tested bacte- ria ranged from 13.0 to 17.5 mm, and that of the fermentation broth extract ranged from 10.5 to 12.7 ram. Enterobacter cloacae was the most sensitive to both my- celium extract and fermentation broth extract, with the inhibition zone diameters of 17.1 and 12.7 mm and the minimum inhibitory concentrations of 2.5 and 5 mg/mL, respectively. The inhibitory effects of the mycelium extract and the fermentation broth extract against Fusarium graminearum were the greatest, and the in- hibition rates were 89.88% and 69.53%, respectively. [ Conclusion] X. striata has potential antimicrobial activity and deserves to be further studied.

Chemical composition and antimicrobial activity of essential oil isolated from the cultured mycelia of Ganoderma japonicum

Objective：To explore a new natural antibiotic. Methods：The chemical composition of the essential oil from Ganodermajaponicum （G. japonicum）mycelia was analyzed by gas chromatography-mass spectrometry（GC-MS）, The antimicrobial activity of the oil was evaluated against eighteen microorganisms, including bacteria, mildew and yeast by using a disc diffusion method. Furthermore, the minimum inhibitory concentrations（MIC） and the minimum bactericidal concentrations（MBC） of the essential oil against twelve clinical pathogens were determined. Results：The main components of the oil were nerolidol, decadienal, linalool and benzyl alcohol. The antimicrobial results indicated that the oil inhibited all the tested bacterium, especially Methicillin-resistant Staphylococcus aureus （MRSA） in which the antibacterial activity exhibited a MBC of 1.03 mg/ml. Conclusion： The essential oil of G. japonicum mycelium has significant inhibitory activity. It is a potential medicinal resource that can be used as a natural antibiotic.

Study on the synthesis and antimicrobial activity of novel cationic porphyrins

A novel series of quaternary ammonium cationic derivatives based on tetrapyridyl-porphyrin was synthesized. All the compounds were evaluated for their in vitro antibacterial activities against S. aureus, E. coli and P. aeruginosa, and antifungal activities against C. albicans, where microorganisms were exposed and unexposed to the irradiation. The results revealed that some of these compounds, especially, 3a and 4a displayed satisfactory antibacterial activity against Gram-positive bacteria S. aureus and moderate antifungal activity against C. albicans. Unfortunately, Gram-negative bacteria P aeruginasa was resistant to all compounds. The antimicrobial activity was found to be sensitive to the functional groups attached on the aromatic ring and the complex metal in the porphyrin ring, and decreased with the increase of electron-withdrawing capability of the functional groups. These preliminary results suggested that the remarkable antibacterial efficiency against S. aureus makes these substances promising antimicrobial agents.

Antimicrobial activity of alexidine alone and associated with N-acetylcysteine against Enterococcus faecalis biofilm

The purpose of this study was to assess the efficacy of alexidine（ALX）,alone and combined with N-acetylcysteine（NAC）,in eradicating two Enterococcus faecalis strain biofilms.The biofilms of E.faecalis ATCC 29212 and the clinical isolate E.faecalis D1 were grown in the MBEC-high-throughput device for 24 h and were exposed to five twofold dilutions of ALX（2%–0.007 8%）alone and combined with100 mg？mL21NAC,for 1 and 5 min.Eradication was defined as 100%kill of biofilm bacteria.The Student’s t-test was used to compare the efficacy of the associations of the two irrigants.After 1-min contact time,ALX eradicated the biofilms at all concentrations except for 0.007 8%and 0.015 6%–0.007 8%with E.faecalis ATCC 29212 and E.faecalis D1,respectively.Similar results for eradication and concentration were obtained when it was combined with 100 mg？mL21NAC.After 5 min of contact time,ALX alone and combined with NAC eradicated all enterococci biofilms.ALX showed antimicrobial properties against the two E.faecalis strain biofilms tested at very low concentrations,and its combined use with NAC was not seen to enhance its activity.

Antimicrobial activity of kojic acid from endophytic fungus Colletotrichum gloeosporioides isolated from Sonneratia apetala,a mangrove plant of the Sundarbans

Objective: To isolate and evaluate the antimicrobial activity of the active principle(s) from the ethyl acetate(EtOAc) extract of endophytic fungus Colietotrichum gloeosporioides(C.gloeosporioides) isolated from Sonneratia apetala. Methods: Water agar technique was used to isolate the fungus, and both microscopic and molecular techniques were used for identification of the strain. Potato dextrose broth was used to grow the fungus in large-scale. Reversed-phase preparative HPLC analysis was performed to isolate the major active compound, kojic acid. The EtOAc extract and kojic acid were screened for their antimicrobial activity against two Grampositive and two Gram-negative bacteria as well as a fungal strain using the resazurin 96-well microtitre plate antimicrobial assay. Results: The fungus C. gloeosporioides was isolated from the leaves of Sonneratia apetala. Initial identification of the fugal isolate was carried out using spore characteristics observed under the microscope. Subsequently, the ITS1-5.8 S-ITS2 sequencing was employed for species-level identification of the fungus C. gloeosporioides. Five litres of liquid culture of the fungus produced approximately 610 mg of a mixture of secondary metabolites.Kojic acid(1) was isolated as the main secondary metabolite present in the fungal extract, and the structure was confirmed by 1 D, 2 D NMR and mass spectrometry. The EtOAc extract and compound 1 exhibited considerable antimicrobial activity against all tested microorganisms.Whilst the minimum inhibitory concentration(MIC) values from the EtOAc extract ranged between 2.4×10^(-4)mg/mL and 2.5 mg/mL, those of kojic acid(1) were between 0.125 mg/mL and1 mg/mL. The EtOAc extract and kojic acid(1) were most active against Pseudomonas aeruginosa(MIC = 2.4×10^(-4). mg/mL) and Micrococcus luteus(MIC = 0.125 mg/mL), respectively. Conclusions:The results revealed that the endophytic fungus C. gloeosporioides could be a good source of commercially important kojic acid, which exhibited antimicrobial properties.

Chemical profiling and antimicrobial activity of essential oil from Curcuma aeruginosa Roxb.,Curcuma glans K.Larsen&J.Mood and Curcuma cf.xanthorrhiza Roxb.collected in Thailand

Objective: To investigate chemical constituents and new antimicrobial agents among essential oils from the rhizomes of Curcuma aeruginosa(C. aeruginosa) Roxb., Curcuma glans K. Larsen & J. Mood and Curcuma cf. xanthorrhiza Roxb.Methods: The essential oils were obtained by hydro-distillation and analyzed by gas chromatography/mass spectroscopy. Agar-well diffusion assay was used to study the antimicrobial activity and also broth-micro dilution techniques were examined for minimum inhibitory concentration(MIC) against four bacterial strains and yeast.Results: The gas chromatography/mass spectroscopy analysis showed monoterpenes predominantly(88.53%) in the rhizome oil of Curcuma cf. xanthorrhiza. Sesquiterpenes(50.10%) was the most abundant component in the essential oil of C. glans, while monoterpenes(45.55%) and sesquiterpenes(45.81%) were found in C. aeruginosa with a significant amount. The major components of C. aeruginosa were characterized as camphor(29.39%) and germacrone(21.21%). Germacrone(15.76%), b-pinene(9.97%)and camphor(9.96%) were found as major compounds in the rhizome oils of C. glans while a-terpinolene(24.86%) and p-cymen-7-ol(12.17%) were found as major compositions in Curcuma cf. xanthorrhiza. The essential oils were tested against four bacterial strains and yeast. As a result, the rhizome oil of C. aeruginosa exhibited potent activity against Staphylococcus aureus [inhibition zone(21.94 ± 0.24) mm, MIC 125 μg/mL],Bacillus cereus [inhibition zone(20.83 ± 0.36) mm, MIC 125 μg/mL], and Candida albicans [inhibition zone(11.60 ± 0.30) mm, MIC 250 μg/mL].Conclusions: The essential oils from three Curcuma species possessed greater activity against the gram-positive bacteria(Staphylococcus aureus and Bacillus cereus) than gram-negative bacteria(Escherichia coli and Pseudomonas aeruginosa). The results suggest that the essential oils from the fresh rhizome of Curcuma spp. might be a potential source of natural antimicrobial substances.

Antimicrobial activity from Piper sarmentosum Roxb. against rice pathogenic bacteria and fungi

In vitro antimicrobial activity of the dichloromethane and methanol extracts of Piper sarmentosum leaves, fruits, stems, and the essential oil obtained from the fresh leaves together with their constituents were investigated against two rice pathogenic fungi： Rhizoctonia solani （sheath blight causal agent） and Bipolaris oryzae （brown spot causal agent）, and two rice pathogenic bacteria： Xanthomonas oryzae pv. oryzae （Xoo） （bacterial leaf blight causal agent） and pv. oryzicola （Xoc） （bacterial leaf streak causal agent）. Among them, the dichloromethane extracts of the leaves and fruits, and the essential oil showed significantly high potential anti-rice microbial activity. Based on bioassay-guided fractionation of the dichloromethane leave and fruit extracts, myristicin, sarmentine, brachystamide B, brachyamide B, and piperonal were isolated. Moreover, the major constituent of its oil was also myristicin. Myristicin and brachyamide B revealed the highest potent inhibition against R. solani and B. oryzae （half maximal inhibitory concentration （IC5o） of 0.69 and 0.12 mmol L-I）, respectively. Moreover, brachyamide B and piperonal displayed most antibacterial activity against Xoo （MIC/MBC 7.62/1.90 mmol L-1） and Xoc （MIC/MBC 2.59/20.75 mmol L-1）, respectively. Additionally, the essential oil also exhibited the antimicrobial activity against all tested rice pathogenic bacteria and fungi. These compounds and the oil were first evaluated for anti-rice pathogenic microbial activity.

Synthesis, Structure and Antimicrobial Activity of 9,9-Dimethyl-9,10-dihydrospiro[benzo[a]-xanthene-12,3'-indoline]-2',11(8H)-dione

One novel spiro-compound（C_（26）H_（21）NO_3） has been synthesized and characterized by means of NMR spectroscopy,elemental analyses and X-ray diffraction. The single crystal belongs to the monoclinic system,space group P21/c with a = 8.8039（7）,b = 24.123（2）,c = 10.0751（9） ？,β = 108.403（3）°,M3r = 395.44,V = 2030.3（3） ？~3,Z = 4,D_c = 1.294 g/cm,F（000） = 832.0,μ = 0.085 mm^（-1）,R = 0.0801 and wR = 0.2228. The title compound shows good activities against Micrococcus tetragenus,Bacillus cereus,Bacillus subtilis,Staphylococcus aureus,S.albus and Escherichia coli.

Synthesis, Structure and Antimicrobial Activity of a Cyclic Compound [Cu(Pmal)(Dadm)(H_2O)]_2·4H_2O

A new copper compound constructed from H2 Pmal （phenylmalonic acid）, Dadm （4,4-diaminodiphenylmethane） and Cu（Ⅱ） has been synthesized from H2O-EtOH-DMF solution, and is characterized by X-ray single-crystal diffraction and other measurements. The compound crystallizes in the monoclinic system, space group C2/c with a=13.7866（6）, b=10.1557（4）, c=30.9036（17）, β=101.422（5）o, V=4241.2（3）3 , Z=4, Dc=1.547g/cm3 , Mr=987.94, F（000）=2056, μ（MoKα）=1.078 mm-1 , S=1.007, R=0.0453 and wR=0.987 for 2806 observed reflections （I〉2σ （I））. The title compound is characteristic of the cyclic dimeric molecules which further aggregate through hydrogen bonds and π-π interactions to form a 3-D supramolecular network. The microbial activities of the title compound and its reagents are investigated with filter paper discs diffusion method. The results show that the activity of the title compound is slightly better than its precursor reagents.

Synthesis,Crystal Structure and Antimicrobial Activity of a New Ternary Copper(Ⅱ) Complex with p-Chlorophenoxyacetic Acid and 2-Amino Benzothiazole

A novel ternary complex of Cu（pcpa）2（aben）2 （pcpa=p-chlorophenoxyacetic acid anion,aben=2-amino benzothiazole） was synthesized by the reaction of copper acetate,2-amino benzothiazole and p-chlorophenoxyacetic acid.Elemental analysis,IR,UV and X-ray single-crystal diffraction were carried out to determine the composition and crystal structure.The crystal crystalli-zes in the monoclinic system,space group C2/c with a=25.795（4）,b=7.384（3）,c =17.741（6）,β=107.47（2）°,C30H24Cl2CuN4O6S2,Mr=735.09,V=3223.2（18） 3,Z=4,Dc=1.515 Mg/m3,λ（MoKα）=0.71073 ,μ=1.022 mm-1,F（000）=1500,the final R=0.0464 and wR=0.1244.A total of 3218 unique reflections were collected,of which 2228 with I 〉 2σ（I） were observed.The Cu（II） atom is four-coordinated with two carboxylate oxygen atoms of the two pcpa ligands and two nitrogen atoms in thiazole rings of two aben ligands.The analysis of crystal structure shows intermolecular and intramolecular hydrogen bonds between amino-nitrogen atoms of the two aben ligands and carboxylate oxygen atoms of the two pcpa ligands.The antimicrobial properties of the title complex and its two free ligands were tested against representative bacterial and fungal strains.Results show that the antibacterial activity of the complex is less than or equal to that of 2-amino benzothiazole,but for yeasts and moulds,it exhibits excellent inhibitory effect better than that of its two free ligands.

Expression and Purification of Multimeric Natto Peptide in Escherichia coli and Initial Characterization of Its Antimicrobial Activity and Stability

Increasing amounts of antibiotic resistant bacteria have been an emergency problem. Antimicrobial peptides are promising antibiotic alternatives for broad-spectrum antimicrobial activity and nearly no drug resistance. Natto peptide was a new antimicrobial peptide which consisted of 45 amino acids. In this study, to improve the antimicrobial activity of Natto peptide, three repeats of encoding sequences were synthesized and cloned into a pET28 a(+) expression vector, and expressed in Escherichia coli as a soluble protein. Unexpectedly, the purified 3×Natto peptide exhibited antimicrobial activity against Listeria monocytogenes(50 μg/ml) and Salmonella enteriditis(30 μg/ml). Furthermore, the antibacterial spectrum of 3×Natto peptide was not affected by temperature, pH value and proteinase digestion. Taken together, this was the first study proving that 3×Natto peptide could be produced in E. coli as a kind of water dissolve protein, and has great potential for commercial application in the future.

Hybrid Antimicrobial Peptide Buforin Ⅱ-Cecropin B Exhibits Antimicrobial Activity

[ Objective ] This study aimed to investigate the antimicrobial activity of hybrid antimicrobial peptide buforin II-cecropin B. [ Method ] Gene fragment BC encoding the hybrid antimicrobial peptide Buforin II-Cecropin B was synthesized by SOE-PCR with six primers designed according to the published amino acid sequences. Then, the BC gene fragment was ligated into pET32a vector and expressed in BI21. Antimierobial ability of the crude hybrid antimicrobial peptide was detected with the Oxford cup method. [ Result] The BC gene fragment was correctly amplified by SOE-PCR and ligated into pET32a vector, to construct the expression vector pET32-BC. The hybrid antimicrobial peptide buforin II-cecropin B was expressed in BI21 after induction by IPTG. The optimal induction time was 2 h. Antimicrobial activity assay suggested that the hybrid antimicrobial peptide possessed antimicrobial activity to Escherichia coli and Bacillus subtilis. [ Conclusion] The hybrid antimicrobial peptide buforin II-cecropin B exhibited antimicrobial activity to Gram-negative E. coli and Gram-positive B. subtilis strains.

Antioxidant and Antimicrobial Activity of Basil, Thyme and Tarragon Used in Meat Products

Currently, the food industry, including the meat industry, is paying close attention to the use of natural additives as preservatives. From ancient times, the plants are used to treat various diseases, to produce perfumes and as ingredients to improve the taste in food. This paper presents a bibliographic and experimental study of the antioxidant and microbial properties of basil, thyme and tarragon. International scientific papers on the use of these plants in the food industry, including the meat industry, are targeted. The role of basil, thyme and tarragon in the manufacture of functional and stable products in storage is mentioned. Percentage decrease in Salmonella Abony growth under the influence of basil constituted 84.4%, thyme 61.6% and tarragon 76.8% after 48 hours of action and respectively 97.2%, 90.2% and 95.3% after 72 hours of action. The interdependence between the percentage reduction of S. Abony infestation and the concentration of basil, mushrooms and tarragon was respectively: basil (R2 = 0.7725 … 0.7916), thyme (R2 = 0.7733 … 0.7768), tarragon (R2 = 0.7689 … 0.8137).