An algal astaxanthin feeding trial was carried out to investigate the ef fects of natural astaxanthin from Haematococcus pluvialis as feed additives on growth, pigmenting efficacy and antioxidant capacity in blood par...An algal astaxanthin feeding trial was carried out to investigate the ef fects of natural astaxanthin from Haematococcus pluvialis as feed additives on growth, pigmenting efficacy and antioxidant capacity in blood parrot(C ichlasoma citrinellum × C ichlasoma. synspilum). Tissue total antioxidant capacity(TAC), superoxide dismutase(SOD), catalase(CAT) and maleic dialdehyde(MDA) were chosen as measures of its antioxidant capacity. All fish which received an astaxanthin(from micro-algal H. pluvialis) supplemented diet with 400 mg/kg of astaxanthin, after 50 days of feeding, the astaxanthin-fed fish displayed a pinkcolored skin and the control-fed fish displayed a grayish skin. For the growth, the weight gains of controlfed fish and astaxanthin-fed fish were 200% and 300%, respectively. Samples of skin and scales were used for analysis of total carotenoids and astaxanthin content, and fish feeding astaxanthin showed significantly( P <0.05) higher concentrations than the control group, indicating that the pigmentation of this fish had been significantly improved by dietary astaxanthin. Compared with the control fish, pigmented fish had lower SOD, CAT and MDA and higher TAC. It can be concluded that supplementation with dietary astaxanthin could eff ectively enhance growth, skin coloration and the antioxidant capacity of this fish. This study will provide a reference for application of natural astaxanthin from H. pluvialis as feed additives in blood parrot artificial breeding. Our data is also useful in ornamental fish farming, especially when the retentivity of astaxanthin in the skin and scales are involved. It is leading to the possibility of increasing the pigmentation of farmed-fish by adding the powdered form of H. pluvialis to the diet as an ef fective pigment.展开更多
Citrus fruits are rich in phenolic compounds that possess several health benefits.However,few studies have focused on the changes in phenolic compounds in citrus fruits during postharvest storage.This study dynamicall...Citrus fruits are rich in phenolic compounds that possess several health benefits.However,few studies have focused on the changes in phenolic compounds in citrus fruits during postharvest storage.This study dynamically monitored the phenolic content,components and antioxidant activity of‘Tarocco’blood oranges during a period of 12-week cold storage and on-tree storage,respectively.We investigated the alteration mechanism of phenolic compounds in blood oranges by evaluating phenylpropanoid pathway-related enzyme activities and gene expression.Results showed that flavanones were the main phenolic compounds in blood oranges.Both storage methods mainly stimulated the accumulation of phenolic acids to improve total phenolic content,which reached the maximum at week 12.Nonetheless,blood oranges had a higher phenolic content and antioxidant activity under on-tree storage than cold storage.Furthermore,the enzyme activities and gene expression of the phenylpropanoid pathway demonstrated that the accumulation of phenolics in blood oranges during storage was highly related to the activation of the phenylpropanoid pathway.These results demonstrate that on-tree storage is a potential approach for extending the supply period of blood orange from the perspective of phenolic compounds.展开更多
To estimate the antioxidant activities of Peanut polypeptides (PPs) by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide wa...To estimate the antioxidant activities of Peanut polypeptides (PPs) by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the Pyrogallol-Luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PPs was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results shows that PPs had good effect on the scavenging ability of superoxide anion (IC50=9.68±0.12 mg/ml). PPs could scavenge hydroxide radical effectively (the IC50 value was 46.06±0.08 μg/ml). PPs had a good scavenging ability on hydrogen peroxide, which had a relatively low IC50 value (0.17±0.07 mg/ml). PPs (the IC50 value was0.72±0.11 mg/ml) were powerful on the DNA damage preventing effect. PPs possesses a good scavenging potency on ROS in different systems, but different results exist in different systems.展开更多
Obesity, one of the most common health problems, is associated with glucose and lipid metabolism abnormalities, increased cardiovascular risk and oxidative stressI1}. Aging is also associated with an increase in oxida...Obesity, one of the most common health problems, is associated with glucose and lipid metabolism abnormalities, increased cardiovascular risk and oxidative stressI1}. Aging is also associated with an increase in oxidative stress[2]. Considering that older adults are already at risk for oxidant stress, the additional metabolic perturbations of obesity aggravated the susceptibility to oxidant stress. Modulating age-related oxidative stress via dietary interventions could reduce free radical production and significantly improve health in aging.展开更多
Objective:To investigate the hypoglycemic,hypolipidemic and antioxidant activities of aqueous extract of Terminalia paniculata bark(AETPB) in streptozotocin(STZ)-mduced diabetic rats.Methods:Acute toxicity was studied...Objective:To investigate the hypoglycemic,hypolipidemic and antioxidant activities of aqueous extract of Terminalia paniculata bark(AETPB) in streptozotocin(STZ)-mduced diabetic rats.Methods:Acute toxicity was studied in rats after the oral administration of AETPB to determine the dose to assess hypoglycemic activity.In rats,diabetes was induced by injection of STZ(60 mg/kg,i.p.) and diabetes was confirmed 72 h after induction,and then allowed for 14 days to stabilize blood glucose level.In diabetic rats,AETPB was orally given for 28 days and its effect on blood glucose and body weight was determined on a weekly basis.At the end of the experimental day,fasting blood sample was collected to estimate the haemoglobin(Hb),glycosylated haemoglobin(HbA1c),serum creatinine,urea,serum glutamate-pyruvate transaminase(SGPT),serum glutamate-oxaloacetate transaminase(SGOT) and insulin levels. The liver and kidney were collected to determine antioxidants levels in diabetic rats.Results: Oral administration of AETPB did not exhibit toxicity and death at a dose of 2000 mg/kg.AETPB treated diabetic rats significantly(P<0.001,P<0.01 and P<0.05) reduced elevated blood glucose, HbAlc,creatinine,urea,SGPT and SGOT levels when compared with diabetic control rats.The body weight,Hb,insulin and total protein levels were significantly(P<0.001,P<0.01 and P<0.05) increased in diabetic rats treated with AETPB compared to diabetic control rats.In diabetic rats, AETPB treatment significantly reversed abnormal status of antioxidants and lipid profile levels towards near normal levels compared to diabetic control rats.Conclusions:Present study results confirm that AETPB possesses significant hypoglycemic,hypolipidemic and antioxidant activities in diabetic condition.展开更多
Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided ...Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided into two groups: endocrine hypertension(HTN group) that received a combination of prednisone and salt for two weeks and oxidative stress-associated hypertension(HTN-OS group) that received additional induction of L-Nitro Arginine Methyl Esther(L-NAME) for two days. Each group was subdivided into 4 and treated intravenously with the extract 5; 10; and 20 mg/kg, and vehicle control. The systolic blood pressure(SBP), diastolic blood pressure(DBP), and mean arterial pressure(MAP) were recorded. The blood was taken before and at the end of recording for the measurement of serum concentration of nitric oxide(NO). The changes of blood pressure were analyzed by two-way ANOVA while its correlation with NO concentration was analyzed by Pearson's Correlation.Results: The study showed a significant antihypertensive effect of the extract as compared with control group(P < 0.05) in both hypertensive models. Extract in the dose of 5 mg/kg showed the best blood pressure lowering effect. However, the correlation analysis did not show an association between NO increase and blood pressure lowering effect(P > 0.05).Conclusions: The study concludes that C. filiformis extract in the dose of 5 mg/kg exhibits the best blood pressure lowering effect in both animal models. Antihypertensive activity of the extract is not correlated with its antioxidant effect.展开更多
Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory dis-turbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are...Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory dis-turbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with techne-tium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99mTc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99mTc. The data showed significant (P<0.05) alteration of ATI in BC from blood incubated with guava extract.展开更多
Nanotechnology is a rapidly growing field that has elicited much concern due to a variety of applications in different fields such as industry, medicine, and cosmetics. These developments increase the concern among th...Nanotechnology is a rapidly growing field that has elicited much concern due to a variety of applications in different fields such as industry, medicine, and cosmetics. These developments increase the concern among the general population. Hence, there is an urgent need to explore the possible human health effects of these nanomaterials. The present study is aimed to evaluate the cytotoxic and genotoxic effects of iron oxide nanoparticles (IONPs) in-vivo. In order to study the toxic effects, Wistar rats were administered intravenously with various doses of IONPs (Fe2O3) through caudal vein once in a week for 28 days, and various biochemical assays such as antioxidant enzymes activity (SOD, CAT, and GSH), lipid peroxidation, DNA damage and hematological parameters were evaluated. Genotoxicity was evaluated by comet assay and oxidative stress was measured by anti-oxidant enzymes. The results reveal that IONPs alter hematological factor such as RBC counts, WBC counts, neutrophils, monocytes and hemoglobin. A dose-dependent inhibition (p < 0.05) of antioxidant enzymes was found, and meanwhile the level of MDA elevated significantly (p < 0.05) in IONPs treated groups in dose-dependent manner;however comet assay results indicate that IONPs did not induce any significant DNA damage. The present study concluded that IONP affects inflammatory response, which induces the oxidative stress and may adversely affect the cellular function.展开更多
Glossogyne tenuifolia (GT) is the traditional herbal tea in Penghu Island, Taiwan. Recent research has shown that the active components in GT are potential inhibitors of α-glucosidase. The present study investigated ...Glossogyne tenuifolia (GT) is the traditional herbal tea in Penghu Island, Taiwan. Recent research has shown that the active components in GT are potential inhibitors of α-glucosidase. The present study investigated that whether or not GT could improve the status of type 2 diabetes mellitus. Male Wistar rats aged eight weeks were induced to be hyperglycemic by subcutaneous injection of streptozotocin-nicotinamide (STZ-NA) and combination of high-fat diet (HFD). The animals were given GT extracts at the low dose or high dose, or the anti-diabetic drug (acarbose), in drinking water for 4 weeks. The results showed that hot water extracts from GT resulted in significantly decreases in fasting blood glucose at the 1st and 2nd weeks, fasting insulin levels at the 2nd week, 1 hour postprandial blood glucose after the starch tolerance test on Day 23 and blood glucose levels after oral glucose tolerance test (OGTT) at the 60th minute on Day 25. In addition, diabetic rats treated with GT extracts from hot water for 4 weeks displayed significantly decreased thiobarbituric acid reactive substances (TBARS) in the serum, liver and kidney, serum total cholesterol, fasting insulin levels and homeostasis model assessment for insulin resistance (HOMA-IR). Overall, these results demonstrate that the hot water extracts of GT might improve the progression of diabetes and decrease oxidative stress in STZ-NA-induced diabetic rats.展开更多
Marine mammals undergo cycles of tissue ischemia and reperfusion during the dive response.Reperfusion injury can result in oxidative tissue damage and the activation of a pro-inflammatory immune response.The risk of o...Marine mammals undergo cycles of tissue ischemia and reperfusion during the dive response.Reperfusion injury can result in oxidative tissue damage and the activation of a pro-inflammatory immune response.The risk of oxidative damage is reduced by antioxidants.Our hypothesis is that the reported higher antioxidant defenses within marine mammal tissues provide additional protection in situations that produce oxidative stress,like inflammation,in comparison to terrestrial mammal tissues.Leukocytes were isolated from the whole blood of Pacific bottlenose dolphins(Tursiops truncatus gilli)and humans(Homo sapiens)and were exposed to lipopolysaccharides(LPS,10μg/mL)in vitro to simulate a pro-inflammatory challenge.Oxidative stress indicators,including superoxide radical(O_(2)^(•−))production,activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx),glutathione reductase(GR),and glutathione S-transferase(GST),as well as oxidative protein damage,were quantified by spectrophotometry.Following 48 h under experimental conditions,bottlenose dolphin leukocytes produced 1.9 times more O_(2)^(•−)but displayed 2.0 times lower protein carbonyl concentrations compared to human leukocytes.Following 48 h under experimental conditions,bottlenose dolphin leukocytes displayed 7.9,2.0,11.1,and 3.3 times more activities of CAT,GPx,GR,and GST,respectively,compared to human leukocytes.These results suggest that,under cell culture conditions,the antioxidant defenses in bottlenose dolphin leukocytes provide additional protection against pro-inflammatory challenges in comparison to human leukocytes,likely as an adaptive advantage.展开更多
A scheme of the processes in Fenton solution with various substances is offered, and the channels of light formation registered by the luminometer are analyzed. Under the proposed scheme we discuss the possibilities o...A scheme of the processes in Fenton solution with various substances is offered, and the channels of light formation registered by the luminometer are analyzed. Under the proposed scheme we discuss the possibilities of studying the properties of antioxidants and prooxidants. Oxidation of alanine, albumin and sodium oxalate have been taken as an example. The properties of ascorbic acid and the mechanism of display of its oxidant and prooxidant properties are analyzed herewith. Methodical questions of the chemiluminescence research in Fenton solution such as the selection of reagents concentration, water preparation and the effect of the background radiation have been considered in this study as well.展开更多
The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water ...The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water (WtF) from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence (CL) assay and hydroxyl free radical-initiated chemiluminescence (CL) assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.展开更多
基金Supported by the Xiamen Scientific and Technologic Projects(XSTP)(Nos.3052Z20031086,3052Z20123004)the project of Xiamen Southern Ocean Technology Center of China(No.14CZP035HJ09)+2 种基金partly funded by the Marine Science Base Scientific Research Training and Scientific Research Ability Enhancement Project of Xiamen University(No.J1210050)the National Marine Commonweal Research Program,China(No.201205020-2)the XMU Training Program of Innovation and Enterpreneurship for Undergraduates(No.2016X0619)
文摘An algal astaxanthin feeding trial was carried out to investigate the ef fects of natural astaxanthin from Haematococcus pluvialis as feed additives on growth, pigmenting efficacy and antioxidant capacity in blood parrot(C ichlasoma citrinellum × C ichlasoma. synspilum). Tissue total antioxidant capacity(TAC), superoxide dismutase(SOD), catalase(CAT) and maleic dialdehyde(MDA) were chosen as measures of its antioxidant capacity. All fish which received an astaxanthin(from micro-algal H. pluvialis) supplemented diet with 400 mg/kg of astaxanthin, after 50 days of feeding, the astaxanthin-fed fish displayed a pinkcolored skin and the control-fed fish displayed a grayish skin. For the growth, the weight gains of controlfed fish and astaxanthin-fed fish were 200% and 300%, respectively. Samples of skin and scales were used for analysis of total carotenoids and astaxanthin content, and fish feeding astaxanthin showed significantly( P <0.05) higher concentrations than the control group, indicating that the pigmentation of this fish had been significantly improved by dietary astaxanthin. Compared with the control fish, pigmented fish had lower SOD, CAT and MDA and higher TAC. It can be concluded that supplementation with dietary astaxanthin could eff ectively enhance growth, skin coloration and the antioxidant capacity of this fish. This study will provide a reference for application of natural astaxanthin from H. pluvialis as feed additives in blood parrot artificial breeding. Our data is also useful in ornamental fish farming, especially when the retentivity of astaxanthin in the skin and scales are involved. It is leading to the possibility of increasing the pigmentation of farmed-fish by adding the powdered form of H. pluvialis to the diet as an ef fective pigment.
基金the National Key R&D Program of China(2018YFD0401301)the Chongqing Postgraduate Scientific Research Innovation Project,China(CYS18120).
文摘Citrus fruits are rich in phenolic compounds that possess several health benefits.However,few studies have focused on the changes in phenolic compounds in citrus fruits during postharvest storage.This study dynamically monitored the phenolic content,components and antioxidant activity of‘Tarocco’blood oranges during a period of 12-week cold storage and on-tree storage,respectively.We investigated the alteration mechanism of phenolic compounds in blood oranges by evaluating phenylpropanoid pathway-related enzyme activities and gene expression.Results showed that flavanones were the main phenolic compounds in blood oranges.Both storage methods mainly stimulated the accumulation of phenolic acids to improve total phenolic content,which reached the maximum at week 12.Nonetheless,blood oranges had a higher phenolic content and antioxidant activity under on-tree storage than cold storage.Furthermore,the enzyme activities and gene expression of the phenylpropanoid pathway demonstrated that the accumulation of phenolics in blood oranges during storage was highly related to the activation of the phenylpropanoid pathway.These results demonstrate that on-tree storage is a potential approach for extending the supply period of blood orange from the perspective of phenolic compounds.
文摘To estimate the antioxidant activities of Peanut polypeptides (PPs) by using a chemiluminescence (CL) method in vitro. The scavenging ability of PPs on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the Pyrogallol-Luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PPs was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results shows that PPs had good effect on the scavenging ability of superoxide anion (IC50=9.68±0.12 mg/ml). PPs could scavenge hydroxide radical effectively (the IC50 value was 46.06±0.08 μg/ml). PPs had a good scavenging ability on hydrogen peroxide, which had a relatively low IC50 value (0.17±0.07 mg/ml). PPs (the IC50 value was0.72±0.11 mg/ml) were powerful on the DNA damage preventing effect. PPs possesses a good scavenging potency on ROS in different systems, but different results exist in different systems.
基金supported by the French Foreign Office(International Research Extension Grant TASSILI 08MDU723)the Algerian Research Project(PNR,2011)
文摘Obesity, one of the most common health problems, is associated with glucose and lipid metabolism abnormalities, increased cardiovascular risk and oxidative stressI1}. Aging is also associated with an increase in oxidative stress[2]. Considering that older adults are already at risk for oxidant stress, the additional metabolic perturbations of obesity aggravated the susceptibility to oxidant stress. Modulating age-related oxidative stress via dietary interventions could reduce free radical production and significantly improve health in aging.
基金financially supported by Canara Bank,Zamin Uthukuli(grant No.133765125313)
文摘Objective:To investigate the hypoglycemic,hypolipidemic and antioxidant activities of aqueous extract of Terminalia paniculata bark(AETPB) in streptozotocin(STZ)-mduced diabetic rats.Methods:Acute toxicity was studied in rats after the oral administration of AETPB to determine the dose to assess hypoglycemic activity.In rats,diabetes was induced by injection of STZ(60 mg/kg,i.p.) and diabetes was confirmed 72 h after induction,and then allowed for 14 days to stabilize blood glucose level.In diabetic rats,AETPB was orally given for 28 days and its effect on blood glucose and body weight was determined on a weekly basis.At the end of the experimental day,fasting blood sample was collected to estimate the haemoglobin(Hb),glycosylated haemoglobin(HbA1c),serum creatinine,urea,serum glutamate-pyruvate transaminase(SGPT),serum glutamate-oxaloacetate transaminase(SGOT) and insulin levels. The liver and kidney were collected to determine antioxidants levels in diabetic rats.Results: Oral administration of AETPB did not exhibit toxicity and death at a dose of 2000 mg/kg.AETPB treated diabetic rats significantly(P<0.001,P<0.01 and P<0.05) reduced elevated blood glucose, HbAlc,creatinine,urea,SGPT and SGOT levels when compared with diabetic control rats.The body weight,Hb,insulin and total protein levels were significantly(P<0.001,P<0.01 and P<0.05) increased in diabetic rats treated with AETPB compared to diabetic control rats.In diabetic rats, AETPB treatment significantly reversed abnormal status of antioxidants and lipid profile levels towards near normal levels compared to diabetic control rats.Conclusions:Present study results confirm that AETPB possesses significant hypoglycemic,hypolipidemic and antioxidant activities in diabetic condition.
基金financially supported by Ministry of National Education and Culture, The Republic of Indonesia under the scheme of Fundamental Research [grant number 02/UN.16/PLFD/2013]
文摘Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided into two groups: endocrine hypertension(HTN group) that received a combination of prednisone and salt for two weeks and oxidative stress-associated hypertension(HTN-OS group) that received additional induction of L-Nitro Arginine Methyl Esther(L-NAME) for two days. Each group was subdivided into 4 and treated intravenously with the extract 5; 10; and 20 mg/kg, and vehicle control. The systolic blood pressure(SBP), diastolic blood pressure(DBP), and mean arterial pressure(MAP) were recorded. The blood was taken before and at the end of recording for the measurement of serum concentration of nitric oxide(NO). The changes of blood pressure were analyzed by two-way ANOVA while its correlation with NO concentration was analyzed by Pearson's Correlation.Results: The study showed a significant antihypertensive effect of the extract as compared with control group(P < 0.05) in both hypertensive models. Extract in the dose of 5 mg/kg showed the best blood pressure lowering effect. However, the correlation analysis did not show an association between NO increase and blood pressure lowering effect(P > 0.05).Conclusions: The study concludes that C. filiformis extract in the dose of 5 mg/kg exhibits the best blood pressure lowering effect in both animal models. Antihypertensive activity of the extract is not correlated with its antioxidant effect.
基金Project supported by CAPES, CNPq and FAPERJ, Brazil
文摘Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory dis-turbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with techne-tium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99mTc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99mTc. The data showed significant (P<0.05) alteration of ATI in BC from blood incubated with guava extract.
文摘Nanotechnology is a rapidly growing field that has elicited much concern due to a variety of applications in different fields such as industry, medicine, and cosmetics. These developments increase the concern among the general population. Hence, there is an urgent need to explore the possible human health effects of these nanomaterials. The present study is aimed to evaluate the cytotoxic and genotoxic effects of iron oxide nanoparticles (IONPs) in-vivo. In order to study the toxic effects, Wistar rats were administered intravenously with various doses of IONPs (Fe2O3) through caudal vein once in a week for 28 days, and various biochemical assays such as antioxidant enzymes activity (SOD, CAT, and GSH), lipid peroxidation, DNA damage and hematological parameters were evaluated. Genotoxicity was evaluated by comet assay and oxidative stress was measured by anti-oxidant enzymes. The results reveal that IONPs alter hematological factor such as RBC counts, WBC counts, neutrophils, monocytes and hemoglobin. A dose-dependent inhibition (p < 0.05) of antioxidant enzymes was found, and meanwhile the level of MDA elevated significantly (p < 0.05) in IONPs treated groups in dose-dependent manner;however comet assay results indicate that IONPs did not induce any significant DNA damage. The present study concluded that IONP affects inflammatory response, which induces the oxidative stress and may adversely affect the cellular function.
文摘Glossogyne tenuifolia (GT) is the traditional herbal tea in Penghu Island, Taiwan. Recent research has shown that the active components in GT are potential inhibitors of α-glucosidase. The present study investigated that whether or not GT could improve the status of type 2 diabetes mellitus. Male Wistar rats aged eight weeks were induced to be hyperglycemic by subcutaneous injection of streptozotocin-nicotinamide (STZ-NA) and combination of high-fat diet (HFD). The animals were given GT extracts at the low dose or high dose, or the anti-diabetic drug (acarbose), in drinking water for 4 weeks. The results showed that hot water extracts from GT resulted in significantly decreases in fasting blood glucose at the 1st and 2nd weeks, fasting insulin levels at the 2nd week, 1 hour postprandial blood glucose after the starch tolerance test on Day 23 and blood glucose levels after oral glucose tolerance test (OGTT) at the 60th minute on Day 25. In addition, diabetic rats treated with GT extracts from hot water for 4 weeks displayed significantly decreased thiobarbituric acid reactive substances (TBARS) in the serum, liver and kidney, serum total cholesterol, fasting insulin levels and homeostasis model assessment for insulin resistance (HOMA-IR). Overall, these results demonstrate that the hot water extracts of GT might improve the progression of diabetes and decrease oxidative stress in STZ-NA-induced diabetic rats.
基金Funding for this project was received from CONACyT[Project CB-2016-01-283669]and CIBNOR(Línea Estratégica II.Estrés Oxidativo)TES received a CONACYT graduate studies scholarship(CVU No.922584).
文摘Marine mammals undergo cycles of tissue ischemia and reperfusion during the dive response.Reperfusion injury can result in oxidative tissue damage and the activation of a pro-inflammatory immune response.The risk of oxidative damage is reduced by antioxidants.Our hypothesis is that the reported higher antioxidant defenses within marine mammal tissues provide additional protection in situations that produce oxidative stress,like inflammation,in comparison to terrestrial mammal tissues.Leukocytes were isolated from the whole blood of Pacific bottlenose dolphins(Tursiops truncatus gilli)and humans(Homo sapiens)and were exposed to lipopolysaccharides(LPS,10μg/mL)in vitro to simulate a pro-inflammatory challenge.Oxidative stress indicators,including superoxide radical(O_(2)^(•−))production,activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx),glutathione reductase(GR),and glutathione S-transferase(GST),as well as oxidative protein damage,were quantified by spectrophotometry.Following 48 h under experimental conditions,bottlenose dolphin leukocytes produced 1.9 times more O_(2)^(•−)but displayed 2.0 times lower protein carbonyl concentrations compared to human leukocytes.Following 48 h under experimental conditions,bottlenose dolphin leukocytes displayed 7.9,2.0,11.1,and 3.3 times more activities of CAT,GPx,GR,and GST,respectively,compared to human leukocytes.These results suggest that,under cell culture conditions,the antioxidant defenses in bottlenose dolphin leukocytes provide additional protection against pro-inflammatory challenges in comparison to human leukocytes,likely as an adaptive advantage.
文摘A scheme of the processes in Fenton solution with various substances is offered, and the channels of light formation registered by the luminometer are analyzed. Under the proposed scheme we discuss the possibilities of studying the properties of antioxidants and prooxidants. Oxidation of alanine, albumin and sodium oxalate have been taken as an example. The properties of ascorbic acid and the mechanism of display of its oxidant and prooxidant properties are analyzed herewith. Methodical questions of the chemiluminescence research in Fenton solution such as the selection of reagents concentration, water preparation and the effect of the background radiation have been considered in this study as well.
文摘The three water-soluble polysaccharides (PI, PII and Pill), the ethyl acetate extraction (EA) and its five fractions of petroleum ether (PeF), ethyl acetate (EaF), butanol (BuF), methanol (MeF) and water (WtF) from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence (CL) assay and hydroxyl free radical-initiated chemiluminescence (CL) assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.