Polyphenols Content, Antioxidant and Antimicrobial Activity of Ethanol Extracts from the Aerial Part of Rock Rose （Hefianthemum nummularium） Species

This study aimed to evaluate polyphenols content, antioxidant and antimicrobial activities of ethanol extract from rock rose （Helianthemum nummularium （L.） Mill.）. Plant material has been harvested in July from Romanian Carpathian Mountains. The ethanolic extract （70%, v/v） was prepared from the aerial part of rock rose species. High performance thin layer chromatography （HPTLC） analysis of ethanol extract and subsequently hydrolysed sample indicated that quercetin glycosides were the major polyphenolic compounds, and kaempferol derivates, chlorogenic and gallic phenylcarboxylic acids are also being present in the polar extracts. Chemiluminescence assay in luminol/H：O2 system indicated very high antioxidant activity of the ethanolic type of extract （ICs0 = 1.27 ~tg/mL）, while microbiological studies （cylinder method in plates） indicated certain antimicrobial activity （measuring from 12.5 mm to 21.5 mm of the diameter of the inhibition zone） of the propylene glycol （20%, v/v） standardized extract （5 mg gallic acid equivalents/mL sample） against several standard bacterial strains, Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739, Salmonella typhimurium ATCC 14028 and Salmonella enterritidis ATCC 13076, respectively. Therefore, based on the augmented antioxidant effect of the rock rose polar extracts, there can be found numerous applications in the pharmaceutical industry, but also in non-pharmaceutical fields, for example in cosmetic and hygiene products areas, due to certain antimicrobial properties.

Chemical composition,antioxidant and antimicrobial activities of the essential oil of Santolina chamaecyparissus L.of Algeria

Objective:To investigate the chemical composition,antioxidant and antimicrobial activities of essential oil of Santolina chamaecyparissus L.cultivated in Algeria.Methods:The chemical composition of hydrodistilled essential oil from flowering aerial parts has been analyzed by gas chromatograph and gas chromatograph-mass spectrometer techniques.The antioxidant activities conducted by 2,2-diphenyl-1-picrylhydrazyl scavenging test andβ-carotene bleaching test.The antimicrobial activity was realised by agar disc diffusion method and cornell medical index was determined by agar dilution method in solid medium.Results:About 36 components accounting more than 82%of the total oil were identified.Oxygenated monoterpenes was the main fraction(54.66%)and was represented by artemisia ketone(40.33%)as major component of this oil.The other major constituents were(Z)-thujone(9.82%),(2Z,6E)-farnesol(7.30%)and limonene(6.87%)and concerning antiradical scavenging test(2,2-diphenyl-1-picrylhydrazyl)had demonstrated a weak activity obtained with an EC_(50) of(43.01±8.04)and moderate activity for β-carotene bleaching test(47.00±3.13)at 120 min.In the other hand,this oil was found effective against all tested strains except Pseudomonas aeruginosa and Aspergillus species.This activity was ranging from 10 mm to 24 mm with the lowest minimum inhibitory concentration value between under 0.070μg/mL to upper 9μg/mL.Conclusions:The results provided evidence that the studied plant might indeed be potential sources of natural antioxidant and antimicrobial agents.