Objective: To study the effects of a new Chinese herb AT-1 on the tumor cell proliferation and metastasis in vitro. Methods: Tumor cell proliferation activity was tested by MTT. The ability of tumor cell invasion and...Objective: To study the effects of a new Chinese herb AT-1 on the tumor cell proliferation and metastasis in vitro. Methods: Tumor cell proliferation activity was tested by MTT. The ability of tumor cell invasion and migration was assayed by counting the number of tumor cells going throw matrigel. The expression changes of CD44 genes in PG cells treated with AT-1 were tested by FACS. Results: Compared with the control, the proliferation activity of the cells treated with the At-1 was restrained. The invasion and migration ability of PG cells and the expression of the cell adherence related gene CD44 was decreased treatment with AT-1. Conclusion: AT-1 is a new antitumor proliferation and metastasis agent. Its antitumor metastasis effect might be achieved by decreasing the expression of the cell adherence-associate gene CD44.展开更多
Objective: To study the efficiency and the mechanism of QI-LE polysaccharide (QI-LE), a new compoundextracted from one kind of Chinese herbs with antiplatelet activity, against tumor metastasis in mice. Methods: QI-LE...Objective: To study the efficiency and the mechanism of QI-LE polysaccharide (QI-LE), a new compoundextracted from one kind of Chinese herbs with antiplatelet activity, against tumor metastasis in mice. Methods: QI-LE was prepared by our Lab. C57BL/6 female micetransplanted with H22 liver cancer cells were administered with the agent. Then the tumor nodules, pulmonarymetastasis rate, and tumor cell proliferation activity were checked. Proliferation changes of tumor cells treated with different concentrations of QI-LE were also tested. Results: High and middle dosage of QI-LE could inhibit both the lymphatic and blood systemic tumor pulmonary metastases significantly, but not the tumor cell proliferation in vitroConclusion: QI-LE has antitumor metastasis activity, thus may be a new candidates for antitumor metastases agents.展开更多
文摘Objective: To study the effects of a new Chinese herb AT-1 on the tumor cell proliferation and metastasis in vitro. Methods: Tumor cell proliferation activity was tested by MTT. The ability of tumor cell invasion and migration was assayed by counting the number of tumor cells going throw matrigel. The expression changes of CD44 genes in PG cells treated with AT-1 were tested by FACS. Results: Compared with the control, the proliferation activity of the cells treated with the At-1 was restrained. The invasion and migration ability of PG cells and the expression of the cell adherence related gene CD44 was decreased treatment with AT-1. Conclusion: AT-1 is a new antitumor proliferation and metastasis agent. Its antitumor metastasis effect might be achieved by decreasing the expression of the cell adherence-associate gene CD44.
基金This work was supported by the ScienceFoundation from Health Department of Shandong Province (No. 2001CAICDAI) and Chinese Medicine Administration Bureau of Shandong Province(No. 99-46).
文摘Objective: To study the efficiency and the mechanism of QI-LE polysaccharide (QI-LE), a new compoundextracted from one kind of Chinese herbs with antiplatelet activity, against tumor metastasis in mice. Methods: QI-LE was prepared by our Lab. C57BL/6 female micetransplanted with H22 liver cancer cells were administered with the agent. Then the tumor nodules, pulmonarymetastasis rate, and tumor cell proliferation activity were checked. Proliferation changes of tumor cells treated with different concentrations of QI-LE were also tested. Results: High and middle dosage of QI-LE could inhibit both the lymphatic and blood systemic tumor pulmonary metastases significantly, but not the tumor cell proliferation in vitroConclusion: QI-LE has antitumor metastasis activity, thus may be a new candidates for antitumor metastases agents.
