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Congenital penile curvature: long-term results of operative treatment using the plication procedure 被引量:3
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作者 S.-S.Lee E.Meng +4 位作者 F.-P.Chuang C.-Y.Yen S.-Y.Chang D.-S.Yu G.-H.Sun 《Asian Journal of Andrology》 SCIE CAS CSCD 2004年第3期273-276,共4页
Aim: To determine the long-term outcome, effectiveness and patient satisfaction of congenital penile curvature correction by plication of tunica albuginea. Methods: From January 1992 to January 2002, 106 young patient... Aim: To determine the long-term outcome, effectiveness and patient satisfaction of congenital penile curvature correction by plication of tunica albuginea. Methods: From January 1992 to January 2002, 106 young patients underwent surgical correction of congenital penile curvature by corporeal plication. Indications for operation were difficult or impossible vaginal penetration and cosmetic problems. The technique of corporeal plication consists of placing longitudinal plication sutures of 2-zero braided polyester on the convex side of the curvature until the curvature is corrected when erection is artificially induced. Results of this procedure were obtained by retrospective chart reviews and questionnaires via mail. Long-term follow-up ranged from 11 to 132 (mean 69.3) months and data were available for 68 patients. Results: Penile straightening was excellent in 62 patients (91 %) and good with less than 15 degree of residual curvature in 6 patients (9 %). Sixty-seven patients reported no change in erectile rigidity or maintenance postoperatively, while 1 described early detumescence. Shortening of the penis without functional problems was noted by 26 patients (38 %). Thirty-Five patients (51 %) reported feeling palpable indurations (suture knots) on the penis. Temporary numbness of glans penis was described in 3 patients. Overall, 60 patients were very satisfied, 6 satisfied, 2 unsatisfied. Conclusion: Corporeal plication is an effective and durable procedure with a high rate of patient satisfaction. 展开更多
关键词 penile curvature TREATMENT plication procedure
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Long-term Safety Observations on Side Effects and Com -plications of Non-surgical Sterilization by Chem ical In-stillation of the Fallopian Tubal 被引量:1
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作者 LIU Yun-rong(刘云嵘) WU Yu-hao(吴裕浩) +3 位作者 CHEN Xue-huang(陈学煌) HUANGZhen-jia(黄真嘉) XU Duo(徐铎) CAIGuang-zong(蔡光宗) 《Journal of Reproduction and Contraception》 CAS 1999年第1期18-26,共9页
The present objective is to observe the side effects and complications of fem ale tubalsterilization by phenol-atabrinepaste (PAP) and phenolm ucilage (PM). 1 705 eligible w om en w ere divided random ly into tw o g... The present objective is to observe the side effects and complications of fem ale tubalsterilization by phenol-atabrinepaste (PAP) and phenolm ucilage (PM). 1 705 eligible w om en w ere divided random ly into tw o groups, 871 usinig PAP and 834 w om en using PM. Results: Thefeverratesw ere8.0and 4.4( P< 0.01 ) respec- tively in group PAP and group PM. Infection rates of the appendages w ere 2.3 and 1.2 respectively. The incidence of chem ical peritonitis, w ithin the lim its of pelvic cavity, w ere 1.1and 2.4respectively. Perforation of uterus, pelvic ab- scessand ectopicpregnancy w ere notfound during thisobservation. Slightabdom inal pain after the proceduresw ere 33.6and 30.8respectively. Slightvaginalbleed- ing after the procedure w as 23.2 and 18.0 respectively. Menstruation of the w om en w as not affected by the instillation. This five-year follow -up study neither show ed a positivePap testnor a potentialdeseasecaused by thesterilizing agents. This technique isa safe and efficientm ethod for fem ale sterilization. 展开更多
关键词 Fem ale tube sterilization by chem icalinstillation Phenol-atabrine paste (PAP) Phenolm ucilage(PM) Multi-center random ized clinicaltrial Side-effectand com plication
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KRT35、KRT39及转录因子AP-1家族相关基因在中卫山羊不同时期皮肤组织中的表达规律研究
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作者 吕江江 马思佳 +5 位作者 杜海东 曹彦龙 罗锐 赵占强 施安 陶金忠 《中国畜牧杂志》 CAS CSCD 北大核心 2024年第11期194-202,共9页
为进一步探究KRT35(Keratin 35)、KRT39(Keratin 39)及转录因子AP-1家族的相关基因在中卫山羊沙毛皮期前后皮肤组织中的表达规律,实验采用实时荧光定量PCR的方法检测中卫山羊0、35、60 d的皮肤组织样品KRT35、KRT39、JUN(Jun Proto-Onco... 为进一步探究KRT35(Keratin 35)、KRT39(Keratin 39)及转录因子AP-1家族的相关基因在中卫山羊沙毛皮期前后皮肤组织中的表达规律,实验采用实时荧光定量PCR的方法检测中卫山羊0、35、60 d的皮肤组织样品KRT35、KRT39、JUN(Jun Proto-Oncogene)、JUNB(JunB Proto-Oncogene)、FOS(Fos Proto-Oncogene)、FOSB(FosB Proto-Oncogene)、ATF3(Activating Transcription Factor 3)基因表达水平。结果表明,0~35 d FOSB相对表达量极显著上升,FOS相对表达量显著上升,KRT39和JUN相对表达量显著下降,KRT35、JUNB和ATF3差异均不显著;0~60 d KRT35相对表达量显著上升,JUN相对表达量显著下降,KRT39、JUNB、FOSB、FOS和ATF3差异均不显著;35~60 d KRT35相对表达量显著上升,FOSB相对表达量显著下降,KRT39、JUNB、JUN、FOS和ATF3差异均不显著。本研究揭示了KRT35、KRT39及转录因子AP-1家族的相关基因在0、35、60 d中卫山羊皮肤组织中的表达规律,为中卫山羊沙毛皮的研究提供了数据基础。 展开更多
关键词 KRT35 KRT39 ap-1 中卫山羊 羊毛弯曲 实时荧光定量PCR
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基于JNK/AP-1信号通路探讨广西毛冬青在放射性脑损伤中的作用机制
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作者 何改改 李婷 +4 位作者 张馨月 孔蔺莎 阮林 王绍军 韦力 《广西医科大学学报》 CAS 2024年第1期58-66,共9页
目的:探讨广西毛冬青(IPH)对放射性脑损伤的改善作用及其机制。方法:将40只SPF级昆明小鼠随机分为对照组、IPH、放射组和放射+IPH组,每组10只。采用γ射线建立放射性脑损伤模型,放射前、后连续灌胃给药14 d。采用Morris水迷宫实验、避... 目的:探讨广西毛冬青(IPH)对放射性脑损伤的改善作用及其机制。方法:将40只SPF级昆明小鼠随机分为对照组、IPH、放射组和放射+IPH组,每组10只。采用γ射线建立放射性脑损伤模型,放射前、后连续灌胃给药14 d。采用Morris水迷宫实验、避暗实验检测小鼠认知功能,苏木精—伊红(HE)染色和尼氏染色观察脑组织病理形态变化,电镜观察胶质细胞超微结构,酶联免疫吸附试验(ELISA)法检测血清白介素-6(IL-6)水平,免疫组织化学染色法检测脑组织c-Jun氨基末端激酶(JNK)、磷酸化(p-)JNK、激活蛋白-1(AP-1)和单核细胞趋化蛋白-1(MCP-1)蛋白表达,免疫荧光检测脑组织星型胶质细胞标记物GFAP和小胶质细胞标记物Iba-1蛋白表达,western blotting法检测脑组织JNK和p-JNK蛋白表达。结果:与对照组相比,放射组小鼠放射后7 d体重减低,血清IL-6含量升高,脑组织AP-1、MCP-1表达水平及p-JNK/JNK比值升高,小胶质细胞Iba-1和GFAP表达水平升高(均P<0.05)。与放射组比较,放射+IPH组小鼠放射后7 d体重增加,血清IL-6含量降低,脑组织AP-1、MCP-1表达水平及p-JNK/JNK比值降低,小胶质细胞Iba-1和GFAP表达水平降低(均P<0.05)。HE染色和尼氏染色显示,放射组大量细胞和神经元出现核固缩现象,小胶质细胞激活,呈圆形;电镜下可见细胞内溶酶体增多,星形胶质细胞细胞核及胞质肿胀;经IPH干预后,小鼠脑组织神经元变性情况和胶质细胞形态明显改善。结论:广西IPH能改善放射性脑损伤小鼠的认知功能,减少小胶质细胞和星形胶质细胞激活,其机制可能与减轻神经炎症反应、抑制JNK/AP-1信号通路有关。 展开更多
关键词 广西毛冬青 放射性脑损伤 JNK/ap-1 炎症反应 认知障碍
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Diaphragmatic Plication: Retrospective Study with 54 Patients 被引量:1
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作者 Sezai Celik Serhan Tanju +2 位作者 Isa Dongel Onur Gurer Alper Toker 《Open Journal of Thoracic Surgery》 2013年第3期87-92,共6页
Objective: This study seeks to evaluate the results of surgery performed according to the etiological causes of diaphragmatic eventration or paralysis. Method: Files of 54 patients (35 males, 19 females;mean age: 39.1... Objective: This study seeks to evaluate the results of surgery performed according to the etiological causes of diaphragmatic eventration or paralysis. Method: Files of 54 patients (35 males, 19 females;mean age: 39.1 ± 3.01 years) who had undergone diaphragm plication between January 2005 and June 2011 in two clinics located in Istanbul were studied in terms of pulmonary spirometry, applied surgical procedure, duration of hospitalization, morbidity, and mortality. Results: In diaphragm plications, observed etiologies were isolated diaphragmatic eventration without phrenic nerve injury (Group 1) in 20 (37%) patients due to blunt thoracic trauma;Group 2 (thymoma in 19 (35.2%) patients;lung cancer in 7 (12.9%) patients;mediastinal tumor in 3 (5.6%) patients) and congenital heart surgery (Group 3) in 5 (9.3%) patients. The mean time to plication was 7.4 months in Group 1. The mean duration of hospitalization was 4 days in Group 1, 6.2 days in Group 2, 11.8 days in Group 3. Mean forced vital capacity, forced expiratory volume at 1 second improved by 17%, 20.3%, respectively (p In Group 1, mean dyspnea-related hospital admission before plication was 2.4 times. At the end of 6 months after plication mean dyspnea-related hospital admission was 0 time. All of them returned to daily activities or their job within 6 months in Group 1. Postoperative mortality was observed in 2 (3.7%) patients in Group 3 while the overall complication rates were 24%. The morbidity rates were 8%, 27.5%, 60%, respectively;and the mortality rates were 0%, 0%, 40%, respectively. Conclusion: As a result, it could be suggested that early and timely performed plications for diaphragmatic eventration improves functional status and assesses a shorter lenght of stay. On the other hand,simultaneously performed plications in intraoperative phrenic nerve resections can be performed with acceptable morbidity and no mortality. In diaphragmatic paralysis due to congenital heart surgery, morbidity and mortality rate is still high. 展开更多
关键词 Diaphragm plication PARALYSIS Eventration Phrenic Nerve Resection
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CLINICAL APPLICATION OF POINT-THROUGH-POINT ACUPUNCTURE
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作者 马元 王春燕 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1992年第2期154-157,共4页
Point-through-point acupuncture is atechnique in which a long needle is insertedin various directions and depths and acts ontwo or more points in treating disease.It iscommonly used in clinical practice.The ap-plicati... Point-through-point acupuncture is atechnique in which a long needle is insertedin various directions and depths and acts ontwo or more points in treating disease.It iscommonly used in clinical practice.The ap-plication of point-through-point acupunc-ture and its effects are summarized as fol-lows. 展开更多
关键词 ACUPUNCTURE NEEDLE plication treating summarized depths DIRECTIONS NEURALGIA THROAT interior
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Laparoscopic Diaphragmatic Plication in Patients with Unilateral Diaphragm Paralysis with Thoracoscopic Verification
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作者 Willem N. Welvaart Kieng H. Ong +2 位作者 Marinus A. Paul Anton Vonk-Noordegraaf Coen A. C. Ottenheijm 《Open Journal of Thoracic Surgery》 2012年第4期104-107,共4页
Diaphragm eventration or paralysis causes elevation of the ipsilateral diaphragm. Some patients have symptoms. The most common symptom is dyspnea. In symptomatic patients with unilateral diaphragm paralysis and dyspne... Diaphragm eventration or paralysis causes elevation of the ipsilateral diaphragm. Some patients have symptoms. The most common symptom is dyspnea. In symptomatic patients with unilateral diaphragm paralysis and dyspnea disproportionate to the degree of physical activity, diaphragm plication is the treatment of choice to relieve dyspnea. We report on our experience with laparoscopic plication of a paralysed hemidiaphragm. The laparoscopic approach is an attractive surgical alternative for the treatment of phrenic nerve palsy. This technique combines the advantage of an excellent field of vision during surgery with a fast postoperative recovery and early discharge from hospital. In accordance to recent literature, we have successfully applied the laparoscopic approach to treat our patients who suffered from a unilateral paralysis of the diaphragm. Preoperative symptoms of exertional dyspnea and orthopnea were significantly reduced. 展开更多
关键词 DIAPHRAGM PARALYSIS DYSPNEA DIAPHRAGM plication Laparoscopy
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Endoventricular Spiral Plication for Ischemic Dilated Cardiomyopathy
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作者 Kayo Sugiyama Yasuhiro Futamura +3 位作者 Hirotaka Watanuki Masaho Okada Genri Tagami Katsuhiko Matsuyama 《Open Journal of Thoracic Surgery》 2019年第1期1-7,共7页
Surgical ventricular restoration (SVR) procedures have been developed;however, their long-term effectiveness remains controversial. Although a series of endoventricular spiral plication (ESP) has been rarely reported ... Surgical ventricular restoration (SVR) procedures have been developed;however, their long-term effectiveness remains controversial. Although a series of endoventricular spiral plication (ESP) has been rarely reported and its long prognosis is still unknown;this method has a unique concept of left ventricular (LV) restoration without artificial patch materials. Here, we describe the case of a patient with ischemic cardiomyopathy and ischemic mitral regurgitation who successfully underwent ESP, mitral valve repair, and coronary artery bypass grafting. ESP was effective in papillary muscle approximation for avoiding heart failure;however, the noted improvement of LV wall thickening might be temporary. 展开更多
关键词 Endoventricular SPIRAL plication ISCHEMIC CARDIOMYOPATHY Surgical Ventricular Restoration ISCHEMIC MITRAL REGURGITATION
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基于TRAF2/AP-1信号通路探讨柴苓合剂对痰瘀互结MS-IR大鼠炎症因子影响
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作者 王晨阳 张业 《辽宁中医药大学学报》 CAS 2024年第6期37-42,共6页
目的基于TRAF2/AP-1信号通路探讨柴苓合剂对痰瘀互结代谢综合征胰岛素抵抗(MS-IR)大鼠糖代谢、脂代谢、炎症因子的部分作用机制。方法48只SPF级SD雄性大鼠随机分为空白组和造模组。造模组大鼠采用高脂高糖饲料喂养,注射小剂量链脲佐菌素... 目的基于TRAF2/AP-1信号通路探讨柴苓合剂对痰瘀互结代谢综合征胰岛素抵抗(MS-IR)大鼠糖代谢、脂代谢、炎症因子的部分作用机制。方法48只SPF级SD雄性大鼠随机分为空白组和造模组。造模组大鼠采用高脂高糖饲料喂养,注射小剂量链脲佐菌素(STZ)(35 mg/kg),同时用物理刺激方法,建立痰瘀互结型MSIR大鼠模型。造模成功后将大鼠随机分为模型组,柴苓合剂低、中、高剂量组,西药组进行干预。给药各组分别进行柴苓合剂、吡格列酮干预治疗3周,空白组、模型组给予等体积的蒸馏水灌服。生化检测大鼠空腹血糖(FBG)、空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);ELISA法检测血清肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)水平;Western Blot检测肝组织TRAF2、JNK、AP-1蛋白表达;HE染色切片,光学显微镜观察用药前后肝细胞损伤、肝细胞坏死、脂质堆积、炎症细胞浸润等情况。结果(1)糖代谢指标水平。与空白组相比,模型组大鼠FBG、FINS、HOMA-IR水平均升高(P<0.01);与模型组相比,柴苓合剂低、中、高剂量组及西药组FBG、FINS、HOMA-IR水平均降低(P<0.01);与西药组比较,柴苓合剂高剂量组FBG、FINS、HOMA-IR水平差异无统计学意义(P>0.05);与柴苓合剂低剂量组比较,柴苓合剂高、中剂量组及西药组FBG、FINS、HOMA-IR水平均降低(P<0.01);与柴苓合剂中剂量组比较,柴苓合剂高剂量组及西药组FBG、FINS、HOMA-IR水平降低(P<0.01)。(2)脂代谢指标水平。与空白组相比,模型组大鼠甘油三酯(TG)、胆固醇(CHO)水平均升高(P<0.01);与模型组相比,柴苓合剂低、中、高剂量组及西药组TG、CHO水平均降低(P<0.01);与西药组相比,柴苓合剂中、高剂量组TG、CHO水平均降低(P<0.05,P<0.01),柴苓合剂低剂量组TG、CHO水平差异无统计学意义(P>0.05);与柴苓合剂低剂量组相比,柴苓合剂中、高剂量组TG、CHO水平均降低(P<0.01);与柴苓合剂中剂量组相比,柴苓合剂高剂量组TG、CHO水平均降低(P<0.01)。(3)TNF-α、IL-6水平。与空白组相比,模型组大鼠TNF-α、IL-6水平均升高(P<0.01);与模型组相比,柴苓合剂低、中、高剂量组及西药组TNF-α、IL-6水平均降低(P<0.01);与西药组相比,柴苓合剂低、中、高剂量组TNF-α、IL-6水平均降低(P<0.01);与柴苓合剂低剂量组相比,柴苓合剂中、高剂量组TNF-α、IL-6水平均降低(P<0.05,P<0.01);与柴苓合剂中剂量组相比,柴苓合剂高剂量组TNF-α、IL-6水平均降低(P<0.01)。(4)肝组织TRAF2/JNK/AP-1信号通路蛋白表达。与空白组相比,模型组大鼠肝组织TRAF2、JNK、AP-1蛋白表达均上调(P<0.01);与模型组比较,柴苓合剂低、中、高剂量组及西药组肝组织TRAF2、JNK、AP-1蛋白表达水平均降低(P<0.05,P<0.01);与西药组比较,柴苓合剂高剂量组肝组织TRAF2、JNK、AP-1蛋白表达水平均降低(P<0.01),柴苓合剂低、中剂量组肝组织TRAF2、JNK、AP-1蛋白表达水平差异无统计学意义(P>0.05);与柴苓合剂低剂量组比较,柴苓合剂高剂量组肝组织TRAF2、JNK、AP-1蛋白表达水平均降低(P<0.01),柴苓合剂中剂量组肝组织AP-1蛋白表达差异无统计学意义(P>0.05),TRAF2、JNK蛋白表达水平均降低(P<0.01);与柴苓合剂中剂量组比较,柴苓合剂高剂量组TRAF2、JNK、AP-1蛋白表达水平均降低(P<0.05,P<0.01)。结论柴苓合剂高剂量能显著改善痰瘀互结MS-IR大鼠糖代谢、脂代谢和炎症因子,其作用机制可能是柴苓合剂通过调控TRAF2蛋白作用于JNK蛋白,引起它的下游反应,又通过JNK的磷酸化作用于AP-1,释放炎症因子,从而TRAF2作用于JNK,然后磷酸化于AP-1使炎症因子降低有关。 展开更多
关键词 代谢综合征 柴苓合剂 痰瘀互结型 TRAF2 JNK ap-1 TNF-α IL-6
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Diaphragmatic Plication for Patients with Acute Phase Phrenic Nerve Paralysis Following Lung Transplantation: A Case Report
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作者 Yasushi Shintani Masato Minami +3 位作者 Masayoshi Inoue Soichiro Funaki Tomohiro Kawamura Meinoshin Okumura 《Open Journal of Organ Transplant Surgery》 2016年第1期1-5,共5页
Phrenic nerve injury can occur as a complication of lung transplantation. A 54-year-old man underwent single-lung transplantation due to interstitial pneumonia. The patient required circulatory support with venoarteri... Phrenic nerve injury can occur as a complication of lung transplantation. A 54-year-old man underwent single-lung transplantation due to interstitial pneumonia. The patient required circulatory support with venoarterial extracorporeal membrane oxygenation and was unable to be weaned from ventilatory support with nitric oxide. Although enhanced CT scanning showed stenotic anastomosis of the right pulmonary artery (PA), pulmonary angiograph findings revealed that PA flow was normal under sedation and considerably decreased with spontaneous breathing. Fluoroscopy showed that the right diaphragm moved inversely to the position of the left diaphragm, indicating that the right phrenic nerve was paralytic. We performed diaphragmatic plication 7 days after lung transplantation and weaning from ventilator support was accomplished soon thereafter. Phrenic nerve dysfunction is an important clinical problem following lung transplantation. In the present case, diaphragmatic plication was effective for treatment of circulatory failure due to phrenic nerve paralysis even in acute phase after lung transplantation. 展开更多
关键词 Lung Transplantation Phrenic Nerve Paralysis Diaphragmatic plication Circulatory Failure
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Isolated Tricuspid Valve Repair and Right Atrial Plication Performed Using a Beating-Heart Technique for Atrial Functional Tricuspid Valve Regurgitation
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作者 Kayo Sugiyama Hirotaka Watanuki +2 位作者 Masato Tochii Daisuke Koiwa Katsuhiko Matsuyama 《Open Journal of Thoracic Surgery》 2023年第2期7-16,共10页
Background: Isolated tricuspid valve disease remains a controversial indication for surgical intervention. Many patients referred for surgery already have a poor clinical condition and an advanced New York Heart Assoc... Background: Isolated tricuspid valve disease remains a controversial indication for surgical intervention. Many patients referred for surgery already have a poor clinical condition and an advanced New York Heart Association functional class. There is no consensus on the optimal surgical technique for this condition, including on whether to perform the procedure on a beating or an arrested heart and whether to perform valve repair or replacement. Methods: We analyzed four case series between 2015 and 2022 in which patients with secondary tricuspid regurgitation (TR) underwent valve repair on a beating heart and right atrial plication for a dilated right atrium. The TRI-SCORE was calculated for each patient. Results: All patients experienced a favorable postoperative course with significant improvements in heart failure symptoms. TR was markedly reduced;however, in one patient with concomitant mitral regurgitation (MR) and a high TRI-SCORE, MR worsened postoperatively. This patient later died from unknown causes due to multiple comorbidities in the late phase. Conclusions: Tricuspid valve repair on a beating heart was effective for improving the cardiac function, and the TRI-SCORE proved useful as a preoperative risk assessment tool. The underlying mechanism by which TR exacerbates MR requires further investigation. 展开更多
关键词 Isolated Tricuspid Valve Surgery Beating-Heart Surgery Right Atrial plication Atrial Functional Tricuspid Valve Regurgitation TRI-SCORE
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Deep Unity of Classic and Quantum Physics at the Space Thermostat Presence with Technical Applications
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作者 M. Ja. Ivanov V. K. Mamaev Guanghua Zheng 《Journal of Applied Mathematics and Physics》 2017年第4期801-812,共12页
The paper demonstrates deep unity of classic and quantum physics at the space thermostat (ST) presence, which fulfilled all space by the temperature T0 = 2.73 K. The ST presents itself the Cosmic Microwave Background ... The paper demonstrates deep unity of classic and quantum physics at the space thermostat (ST) presence, which fulfilled all space by the temperature T0 = 2.73 K. The ST presents itself the Cosmic Microwave Background (CMB). From the main quantum position we consider the ST/CMB as the wave function carrier (“quantum background”). The paper is devoted to ST/CMB medium the classic conservation laws of mass, momentum and energy. We show the soliton like solutions of our classic model correspond to Schrodinger’s quantum solutions, demonstrate the atom hydrogen specter and other quantum peculiarities. The paper contains typical technical examples classic/ quantum simulation at the ST presence. 展开更多
关键词 SPACE THERMOSTAT Conservation LAWS Schrodinger’s Solutions Technical ap-plications
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AP-1对羊毛KRTs的调控研究
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作者 何海龙 高航 +1 位作者 马思佳 陶金忠 《农业科学研究》 2024年第2期84-90,共7页
滩羊是我国优良的地方绵羊品种,其裘皮形成的机理极为复杂,且是出生后35 d左右特有的性状。综述转录因子AP-1的组成、功能结构、作用机理和结合特点,以及角蛋白的分类及表达特点,并对AP-1对羊毛KRTs基因特异性启动子的结合位点进行预测... 滩羊是我国优良的地方绵羊品种,其裘皮形成的机理极为复杂,且是出生后35 d左右特有的性状。综述转录因子AP-1的组成、功能结构、作用机理和结合特点,以及角蛋白的分类及表达特点,并对AP-1对羊毛KRTs基因特异性启动子的结合位点进行预测,为转录因子对羊毛角蛋白基因的调控研究提供有效的参考。 展开更多
关键词 ap-1 角蛋白 基因调控 羊毛性状 滩羊
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转染DPP-4 siRNA或(和)加入SP600125的小鼠肺泡巨噬细胞极化及JNK/AP-1信号通路激活情况观察
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作者 陈阳西 余幼微 +3 位作者 杨帆 陈睦虎 宋其泰 钟武 《山东医药》 CAS 2024年第12期10-14,共5页
目的观察转染二肽基肽酶-4(DPP-4)siRNA或(和)加入c-Jun N-末端激酶(JNK)抑制剂(SP600125)的小鼠肺泡巨噬细胞极化情况、JNK/AP-1信号通路激活情况。方法体外培养小鼠肺泡巨噬细胞(MH-S),并随机分组:Control组转染空载siRNA、siDPP-4组... 目的观察转染二肽基肽酶-4(DPP-4)siRNA或(和)加入c-Jun N-末端激酶(JNK)抑制剂(SP600125)的小鼠肺泡巨噬细胞极化情况、JNK/AP-1信号通路激活情况。方法体外培养小鼠肺泡巨噬细胞(MH-S),并随机分组:Control组转染空载siRNA、siDPP-4组转染DPP-4 siRNA、LPS组转染空载siRNA+LPS、siDPP-4+LPS组转染DPP-4 siRNA+LPS、LPS+SP600125组转染空载siRNA+LPS联合SP600125、siDPP-4+LPS+SP600125组转染DPP-4 siRNA+LPS联合SP600125。采用Western boltting法检测巨噬细胞中M1型和M2型极化标志物CD86、CD206蛋白及JNK、激活蛋白-1(AP-1)转录蛋白(c-Jun、c-Fos)磷酸化,RT-qPCR法检测巨噬细胞中M1型标志物(CD86、TNF-α、iNOS、IL-1β)和M2型标志物[CD206、精氨酸激酶-1(ARG-1)、IL-4、IL-10]mRNA,一氧化氮(NO)测定试剂盒、免疫荧光检测巨噬细胞上清液中M1型促炎因子NO和细胞内活性氧(ROS)生成情况。结果与Control组比较,LPS组M1型促炎型因子NO、ROS生成含量及M1型极化标志物(CD86蛋白及CD86、TNF-α、iNOS、IL-1βmRNA)表达升高,M2型极化标志物(CD206蛋白及CD206、ARG-1、IL-4、IL-10 mRNA)表达降低,P均<0.05。与LPS组比较,siDPP-4+LPS组M1型促炎型因子NO、ROS生成含量及M1型极化标志物(CD86蛋白及CD86、TNF-α、iNOS、IL-1βmRNA)表达升高,M2型极化标志物(CD206蛋白及CD206、ARG-1、IL-4、IL-10 mRNA)表达降低,P均<0.05。与LPS组比较,siDPP-4+LPS组p-JNK/JNK、p-c-Jun/c-Jun、p-c-Fos/c-Fos蛋白磷酸化相对表达量升高,P均<0.05。与siDPP-4+LPS组比较,siDPP-4+LPS+SP600125组p-JNK/JNK、p-c-Jun/c-Jun、p-c-Fos/c-Fos蛋白磷酸化相对表达量降低,P均<0.05。结论转染DPP-4 siRNA可促进LPS诱导的小鼠肺泡巨噬细胞M1型极化,抑制肺泡巨噬细胞M2型极化,并增加JNK、c-Jun、c-Fos蛋白磷酸化表达;加入JNK抑制剂后,可降低由转染DPP-4 siRNA引起的JNK、c-Jun、c-Fos蛋白磷酸化表达升高。转染DPP-4 siRNA促进LPS诱导的小鼠肺泡巨噬细胞M1型极化的作用机制可能与激活JNK/AP-1信号通路有关。 展开更多
关键词 二肽基肽酶-4 巨噬细胞极化 脂多糖 JNK/ap-1信号通路
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美国AP-42排放系数手册简介及其对我国的启示 被引量:9
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作者 易爱华 陈陆霞 +2 位作者 丁峰 赵晓宏 李时蓓 《生态经济》 CSSCI 北大核心 2016年第11期116-119,共4页
通过对美国AP-42排放系数手册的详细解读,阐述了AP-42中排放源分类、涵盖污染物种类和数量、排放因子开发步骤、质量等级、削减效率等内容,并在此基础上结合我国的研究现状,总结提出了AP-42对我国该领域研究的几点启示:迫切需要编制排... 通过对美国AP-42排放系数手册的详细解读,阐述了AP-42中排放源分类、涵盖污染物种类和数量、排放因子开发步骤、质量等级、削减效率等内容,并在此基础上结合我国的研究现状,总结提出了AP-42对我国该领域研究的几点启示:迫切需要编制排放源强核算导则;规范源强核算方法,采用排污系数与其他方法并举的源强核算体系;构建国家主导、企业及地方政府共同参与的源强核算机制;制定全面的污染源分类体系并采用循序渐进的研究思路;制定完善的改进计划并特别关注数据的有效推广及使用等。 展开更多
关键词 ap-42 排放因子 源强核算 启示
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AP-1与NF-κB的活化表达与T细胞的无能 被引量:10
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作者 许桂莲 朱锡华 +1 位作者 杨劲 黄云辉 《中国免疫学杂志》 CAS CSCD 北大核心 2003年第3期154-157,共4页
目的 :探讨无能T细胞IL 2产生的缺乏与转录因子AP 1和NF kappaB的关系。方法 :通过提取活化组和无能组T细胞的核蛋白 ,进行凝胶电泳迁移率变动分析实验 (EMSA) ,检测了转录因子AP 1和NF kappaB的表达情况。结果 :与活化组相比 ,无能T细... 目的 :探讨无能T细胞IL 2产生的缺乏与转录因子AP 1和NF kappaB的关系。方法 :通过提取活化组和无能组T细胞的核蛋白 ,进行凝胶电泳迁移率变动分析实验 (EMSA) ,检测了转录因子AP 1和NF kappaB的表达情况。结果 :与活化组相比 ,无能T细胞中AP 1不但表达水平下降 ,且出现组份缺失现象 ;转录因子NF kappaB在无能T细胞中的表达则高于活化组 ,但均有三种复合物存在。结论 :无能T细胞IL 2产生减少或缺乏可能与转录因子AP 1和NF kappaB表达紊乱 (减弱或增强 )以及组份的缺失有关。 展开更多
关键词 无能 ap-1 NF-KAPPAB 活化表达 T细胞
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EB病毒LMP-1上调鼻咽癌细胞系AP-1的活性 被引量:15
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作者 罗非君 彭小宁 +3 位作者 李晓艳 顾焕华 夏林庆 曹亚 《中国生物化学与分子生物学报》 CAS CSCD 2000年第4期551-555,共5页
为了探讨 EB病毒潜伏膜蛋白 1 ( LMP- 1 )通过信号传导途径介导的致瘤分子机制 ,由此首先构建了 AP- 1报告基因 ,用佛波酯诱导确定了其报道 AP- 1的功能 ;通过建立荧光素酶双报道系统 ,研究了 LMP- 1表达对 AP- 1和 NFκB活性的影响 .... 为了探讨 EB病毒潜伏膜蛋白 1 ( LMP- 1 )通过信号传导途径介导的致瘤分子机制 ,由此首先构建了 AP- 1报告基因 ,用佛波酯诱导确定了其报道 AP- 1的功能 ;通过建立荧光素酶双报道系统 ,研究了 LMP- 1表达对 AP- 1和 NFκB活性的影响 .研究发现 :在 LMP- 1阴性鼻咽癌 ( NPC)细胞系 ,导入 LMP- 1表达质粒后 ,AP- 1和 NFκB的活性均升高 4~ 5倍 ;而在 LMP- 1阳性 NPC细胞系中 ,当导入 LMP- 1反义表达质粒 ,AP- 1和 NFκB的活性则受抑制 ,活性下调 3~ 4倍 .结果表明 ,LMP- 1能上调 NPC细胞系 AP- 1的活性 ,同时再次证实了 LMP- 1能活化 NFκB. 展开更多
关键词 EB病毒 LMP-1 鼻咽癌 ap-1 NFKB 信号转导
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子痫前期胎盘组织中AP-2α与滋养细胞凋亡、侵袭的相关性研究 被引量:7
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作者 周萍 高雪梅 +1 位作者 王瀚 孟红娟 《海南医学院学报》 CAS 2018年第12期1167-1170,共4页
目的:探讨子痫前期胎盘组织中AP-2α与滋养细胞凋亡、侵袭的相关性。方法:选取2015年11月~2017年10月间在本院分娩的子痫前期产妇68例作为子痫前期组,另取同期在本院进行分娩的健康产妇60例作为正常对照组。对比两组胎盘组织中AP-2α... 目的:探讨子痫前期胎盘组织中AP-2α与滋养细胞凋亡、侵袭的相关性。方法:选取2015年11月~2017年10月间在本院分娩的子痫前期产妇68例作为子痫前期组,另取同期在本院进行分娩的健康产妇60例作为正常对照组。对比两组胎盘组织中AP-2α基因、凋亡相关基因、侵袭相关基因表达量的差异,进一步分析子痫前期胎盘组织中AP-2α基因表达量与滋养细胞凋亡、侵袭活性的内在联系。结果:子痫前期胎盘组织中AP-2αmRNA的表达量高于正常对照组;凋亡相关基因Bcl、Cx43 mRNA的表达量低于正常对照组,Bax、Caspase7、metastin mRNA的表达量高于正常对照组;侵袭相关基因HIF-1α、GPR30、MEST、Snail mRNA的表达量低于正常对照组,PEDF、RECK mRNA的表达量高于正常对照组(P<0.05)。Pearson检验发现,子痫前期胎盘组织中AP-2α基因表达量与滋养细胞凋亡相关基因、侵袭相关基因表达量均直接相关。结论:子痫前期产妇胎盘组织中AP-2α基因表达量异常增高,可正向调控滋养细胞的凋亡活力并负向调控其侵袭活力。 展开更多
关键词 子痫前期 ap- 凋亡 侵袭
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DADS诱导人胃癌细胞分化作用中ERK/AP-1通路的改变 被引量:11
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作者 凌晖 宋颖 +2 位作者 曾铁兵 袁静萍 苏琦 《中国药理学通报》 CAS CSCD 北大核心 2003年第11期1235-1238,共4页
目的 探讨ERK/AP 1通路在二烯丙基二硫 (DADS)诱导人胃癌细胞分化中的作用 ,阐明DADS诱导人胃癌细胞分化的分子机制。方法 采用免疫细胞化学、图像分析及WesternBlot等方法 ,观察DADS作用于体外培养的人胃癌MGC80 3细胞前后AP 1成员c ... 目的 探讨ERK/AP 1通路在二烯丙基二硫 (DADS)诱导人胃癌细胞分化中的作用 ,阐明DADS诱导人胃癌细胞分化的分子机制。方法 采用免疫细胞化学、图像分析及WesternBlot等方法 ,观察DADS作用于体外培养的人胃癌MGC80 3细胞前后AP 1成员c fos与c jun表达的改变以及ERKMAPK激酶的变化。结果 免疫细胞化学及图像分析结果显示 ,对照组c fos、c jun表达呈强阳性 ,而处理组呈阴性或弱阳性 ,阳性率明显降低 ,处理组光密度值较对照组明显低 (P <0 0 5 )。WesternBlot结果显示 :从 2 0、2 5、30到 35mg·L-1DADS处理癌细胞 ,DADS呈浓度依赖性抑制人胃癌细胞中ERK的活化 (P <0 0 5 ) ;并且DADS +MEK抑制剂PD980 5 9能完全阻断ERK活化 (P <0 0 5 ) ;时间效应上 ,在DADS刺激后 15~ 30min抑制作用最强 ,2h左右恢复至接近正常 (P <0 0 5 )。结论 ERK/AP 展开更多
关键词 胃肿瘤 二烯丙基二硫 诱导分化 ERK/ap-1
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乳癖霜外用对乳腺增生模型大鼠乳腺组织病理形态学和AP-2α、C-erbB-2表达的影响 被引量:7
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作者 马民 李德辉 +6 位作者 张桂娟 郭慧 廖锐 谭碧珠 曾晓婷 林逸民 黄斯 《中国老年学杂志》 CAS CSCD 北大核心 2012年第4期738-742,共5页
目的研究乳癖霜外用对乳腺增生模型大鼠乳腺组织病理形态学和AP-2α、C-erbB-2蛋白表达水平的影响。方法 36只大鼠随机分为正常对照组、疾病模型组、三苯氧胺软膏组、乳癖霜小、中、大剂量组。通过雌、孕激素联合制造乳腺增生病大鼠模型... 目的研究乳癖霜外用对乳腺增生模型大鼠乳腺组织病理形态学和AP-2α、C-erbB-2蛋白表达水平的影响。方法 36只大鼠随机分为正常对照组、疾病模型组、三苯氧胺软膏组、乳癖霜小、中、大剂量组。通过雌、孕激素联合制造乳腺增生病大鼠模型,造模的同时给予不同的药物进行干预。30 d后观察各组大鼠乳腺组织病理学和脏器系数的改变,放射免疫法检测各组大鼠血清性激素的水平,Western印迹法测定各组大鼠乳腺组织AP-2α蛋白的表达水平,免疫组化测定各组大鼠乳腺组织C-erbB-2蛋白的表达水平。结果乳癖霜外用能降低乳腺增生模型大鼠的乳头直径和高度,改善模型大鼠乳腺组织病理形态学改变,调节血清性激素水平,降低模型大鼠乳腺组织中转录因子AP-2α、癌基因C-erbB-2蛋白的表达水平,与模型组比较有统计学差异(P<0.01或P<0.05)。结论乳癖霜外用对乳腺增生模型大鼠有显著的疗效,其作用机制可能为调节血清性激素水平及调节大鼠乳腺组织转录因子AP-2α、原癌基因C-erbB-2蛋白的表达水平。 展开更多
关键词 乳癖霜 乳腺增生病 性激素 ap- C-erbB-2
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