Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The pr...Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05) associated at the global level with TC,TG,HDL-C, LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with -1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with -1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and -1131T】C, c.553G】T and c.457G】A to detect the interactions of the ApoA5展开更多
目的分析长沙地区汉族人群脑出血患者血脂水平与载脂蛋白B基因(apolipoprotein B,apoB)G12669A多态性的关系。方法收集93例散发性脑出血患者和100例正常对照者的外周血标本。采用氧化酶法测定血清甘油三酯(triglycerides,TG)、总胆固醇(...目的分析长沙地区汉族人群脑出血患者血脂水平与载脂蛋白B基因(apolipoprotein B,apoB)G12669A多态性的关系。方法收集93例散发性脑出血患者和100例正常对照者的外周血标本。采用氧化酶法测定血清甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein cholesterol,HDL)、低密度脂蛋白(low density lipoprotein cholesterol,LDL)水平,酶联免疫吸附法测定LP(a)浓度,免疫比浊法测定apoB100及apoAI浓度;聚合酶链式反应-限制性片段长度多态性分析法(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)和DNA直接测序法检测apoB基因G12669A多态性。结果①长沙地区汉族人群脑出血患者的TC、TG、LDL-C和LP(a)显著高于正常对照组,而HDL-C显著降低(P<0.05);②脑出血组中,G/A基因型的TC、TG、LDL-C较之G/G型显著增高,而HDL-C则显著降低(P<0.05),其他指标无显著性差异(P>0.05);③脑出血组及对照组apoB基因G12669A多态A等位基因频率分别为0.097、0.045,脑出血组apoB基因G12669A多态A等位基因频率显著高于对照组(P<0.05)。结论长沙地区汉族人群脑出血患者的血脂水平升高,可能与apoB基因G12669A多态A等位基因存在相关性。展开更多
文摘Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05) associated at the global level with TC,TG,HDL-C, LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with -1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with -1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and -1131T】C, c.553G】T and c.457G】A to detect the interactions of the ApoA5
文摘目的分析长沙地区汉族人群脑出血患者血脂水平与载脂蛋白B基因(apolipoprotein B,apoB)G12669A多态性的关系。方法收集93例散发性脑出血患者和100例正常对照者的外周血标本。采用氧化酶法测定血清甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein cholesterol,HDL)、低密度脂蛋白(low density lipoprotein cholesterol,LDL)水平,酶联免疫吸附法测定LP(a)浓度,免疫比浊法测定apoB100及apoAI浓度;聚合酶链式反应-限制性片段长度多态性分析法(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)和DNA直接测序法检测apoB基因G12669A多态性。结果①长沙地区汉族人群脑出血患者的TC、TG、LDL-C和LP(a)显著高于正常对照组,而HDL-C显著降低(P<0.05);②脑出血组中,G/A基因型的TC、TG、LDL-C较之G/G型显著增高,而HDL-C则显著降低(P<0.05),其他指标无显著性差异(P>0.05);③脑出血组及对照组apoB基因G12669A多态A等位基因频率分别为0.097、0.045,脑出血组apoB基因G12669A多态A等位基因频率显著高于对照组(P<0.05)。结论长沙地区汉族人群脑出血患者的血脂水平升高,可能与apoB基因G12669A多态A等位基因存在相关性。
