Although it is usually latent on citrus, apple, and pear, apple stem grooving virus(ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic(LYMM) symptoms have been observed on Hua...Although it is usually latent on citrus, apple, and pear, apple stem grooving virus(ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic(LYMM) symptoms have been observed on Huangjinmiyou(HJY) pummelos(Citrus grandis cv. Huangjinmiyou), a commercial variety that is widely cultivated in South China, high throughput sequencing(HTS) was used to find potential pathogens and only three divergent ASGV variants were identified. The three ASGV variants shared 81.03–82.34% genome-wide pairwise identities with each other, and were separately closest to other ASGV variants from different hosts and/or geographical regions, as indicated by viral phylogenies. However, these new variants may have developed from viral interstrain interactions, based on the results of recombination analysis. A large-scale survey using reverse transcription-PCR(RT-PCR) protocols designed for the three ASGV variants revealed a high incidence(92.7–100%) of ASGV in symptomatic HJY trees from 11 major citrusproducing regions in China. None of ASGV were detected in asymptomatic trees. Temperature treatments applied to the symptomatic HJY plants showed that ASGV is sensitive to high temperatures(30–35°C), at which not only the plants recovered, but also the viruses were not detected by RT-PCR, while at low temperatures(20–24°C), both the symptoms and viruses remained detectable. These data show that ASGV is associated with the LYMM disease prevalent on HJY in China, and this is the significant basis especially of taking appropriate measures timely to manage the disease.展开更多
Based on growth level and peroxidase activity,a comparative study and paried-samples t test were made between virus-free Zaosu pear seedlings and the seedlings inoculated with apple stem grooving virus(ASGV). The resu...Based on growth level and peroxidase activity,a comparative study and paried-samples t test were made between virus-free Zaosu pear seedlings and the seedlings inoculated with apple stem grooving virus(ASGV). The results showed : (1) virus-free Zaosu pear seedlings grew better than inoculated ones. Of the seedlings the height,diameter,branches and autumn braches differed statistically significantly; (2)the peroxidase activity of inoculated seedlings was higher than that of virus-free ones,and the peak of peroxidase activity occurred on about d 7 and d 24 after inoculation. Fig 1,Tab 1,Ref展开更多
基金supported by the National Key R&D Program of China(2019YFD1001800)the National Natural Science Foundation of China(32072389)+1 种基金the Innovation Program for Chongqing’s Overseas Returnees(cx2019013)111 Project(B18044)from Ministry of Education(China)。
文摘Although it is usually latent on citrus, apple, and pear, apple stem grooving virus(ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic(LYMM) symptoms have been observed on Huangjinmiyou(HJY) pummelos(Citrus grandis cv. Huangjinmiyou), a commercial variety that is widely cultivated in South China, high throughput sequencing(HTS) was used to find potential pathogens and only three divergent ASGV variants were identified. The three ASGV variants shared 81.03–82.34% genome-wide pairwise identities with each other, and were separately closest to other ASGV variants from different hosts and/or geographical regions, as indicated by viral phylogenies. However, these new variants may have developed from viral interstrain interactions, based on the results of recombination analysis. A large-scale survey using reverse transcription-PCR(RT-PCR) protocols designed for the three ASGV variants revealed a high incidence(92.7–100%) of ASGV in symptomatic HJY trees from 11 major citrusproducing regions in China. None of ASGV were detected in asymptomatic trees. Temperature treatments applied to the symptomatic HJY plants showed that ASGV is sensitive to high temperatures(30–35°C), at which not only the plants recovered, but also the viruses were not detected by RT-PCR, while at low temperatures(20–24°C), both the symptoms and viruses remained detectable. These data show that ASGV is associated with the LYMM disease prevalent on HJY in China, and this is the significant basis especially of taking appropriate measures timely to manage the disease.
文摘Based on growth level and peroxidase activity,a comparative study and paried-samples t test were made between virus-free Zaosu pear seedlings and the seedlings inoculated with apple stem grooving virus(ASGV). The results showed : (1) virus-free Zaosu pear seedlings grew better than inoculated ones. Of the seedlings the height,diameter,branches and autumn braches differed statistically significantly; (2)the peroxidase activity of inoculated seedlings was higher than that of virus-free ones,and the peak of peroxidase activity occurred on about d 7 and d 24 after inoculation. Fig 1,Tab 1,Ref
