Effects of Organic Manure Application with Chemical Fertilizers on Nutrient Absorption and Yield of Rice in Hunan of Southern China

To evaluate the effect of organic manure application with chemical fertilizers on rice yield and soil fertility under long-term double-rice cropping system, a six year field experiment was conducted continually in the paddy soil derived from Quaternary red clay in Hunan Province of southern China. Four different treatments, i.e., no nitrogen with chemical P and K （PK）, swine manure only （M）, N, P and K chemical fertilizers only （NPK）, and half chemical fertilizers combined with half swine manure （NPKM） with four replications were included. Each N, P and K application rate was the same at all the treatments （except the N application rate at PK） and N application rate was 150 kg N ha^-1. All fertilizers were applied to soil tillage layer with once application as baseal fertilizers. The nutrients uptake rate, grain yield, nitrogen use efficiency, and soil organic matter content at each treatment were investigated. The NPKM treatment achieved the highest mean annual yield of 12.2 t ha^-1 （68% higher than that of PK）. Higher dry matter accumulation and nutrients absorption were observed during the middle-late growth period in the NPKM treatment, with higher panicle number per unit and filled-grain number per panicle. Its average nitrogen use efficiency was 36.3% and soil organic matter increased by 18.5% during the experimental period in the NPKM treatment, which were significantly higher than those in the NPK treatment. Organic manure application with chemical fertilizers increased the yield and nitrogen use efficiency of rice, reduced the risk of environmental pollution and improved soil fertility greatly. It could be a good practical technique that protects the environment and raises the rice yield in this region.

Effects of Dairy Cattle Manure and Chemical Fertilizers on Fertility of Soils Grown with Cichorium intybus

[Objective] The aim was to explore recycling utilization of manure of dairy cattle through returning of manures into fields. [Method] Effects of dairy cattle ma- nure and chemical fertilizer on fertility of soils grown with Cichorium intybus were in- vestigated through a pot experiment. [Result] After manure of dairy cattle was ap- plied, it can be concluded that organic matter, total N, total P, alkali-hydrolyzable ni- trogen, available P, activities of urease and invertase in soils increased by 0.14-1.28 times, 43.8%-79.7%, 17.4%-30.8%, 147%-188%, 7 times, 17.2%-38.5%, and 1.36%- 3.34%, respectively. Furthermore, organic matter, total N. urease and invertase activi- ties in group of M7F3 increased most; total P and available P achieved the best in group of M3F7. These indicated that the applied manures of dairy cattle would maintain and improve soil fertility, providing better soils for Cichorium intybus. [Conclusion] The research provides reference for recycling of cattle manures and construction of ecological cyclical pattern of ＂grass planting-cattle breeding-methane fermentation-returning of manures into fields＂.

Influence of Long Term Fertilization with Different Mineral Fertilizers and Farmyard Manure on Some Soil Chemical Properties and Crop Yields *1

A long term fertilization experiment was carried out in an experimental field in Lyczyn near Warsaw, Poland. Application ofmineral fertilizers, especially N fertilizers with and without farmyard manure accelerated the acidification process of the soil. Application of 1.6 t CaO ha -1 every four years was essential to maintenance of the soil pH KCl at 5.5～6.6 and base saturation degree above 60%. Application of 50 t farmyard manure ha -1 every 4 years, which contained 46 kg P and 240 kg K, was sufficient to maintain both the K and P fertility of the soil. Besides, it was beneficial to alleviating soil acidification. As a result of long term unbalanced fertilization, yield responses to N, P and K fertilizers increased significantly with time. The efficiency of N from farmyard manure was found to be comparable to that of N fertilizer during 1988～1991.

Carbon sequestration rate,nitrogen use efficiency and rice yield responses to long-term substitution of chemical fertilizer by organic manure in a rice–rice cropping system

Combined application of chemical fertilizers with organic amendments was recommended as a strategy for improving yield,soil carbon storage,and nutrient use efficiency.However,how the long-term substitution of chemical fertilizer with organic manure affects rice yield,carbon sequestration rate(CSR),and nitrogen use efficiency(NUE)while ensuring environmental safety remains unclear.This study assessed the long-term effect of substituting chemical fertilizer with organic manure on rice yield,CSR,and NUE.It also determined the optimum substitution ratio in the acidic soil of southern China.The treatments were:(i)NPK0,unfertilized control;(ii)NPK1,100%chemical nitrogen,phosphorus,and potassium fertilizer;(iii)NPKM1,70%chemical NPK fertilizer and 30%organic manure;(iv)NPKM2,50%chemical NPK fertilizer and 50%organic manure;and(v)NPKM3,30%chemical NPK fertilizer and 70%organic manure.Milk vetch and pig manure were sources of manure for early and late rice seasons,respectively.The result showed that SOC content was higher in NPKM1,NPKM2,and NPKM3 treatments than in NPK0 and NPK1 treatments.The carbon sequestration rate increased by 140,160,and 280%under NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK1 treatment.Grain yield was 86.1,93.1,93.6,and 96.5%higher under NPK1,NPKM1,NPKM2,and NPKM3 treatments,respectively,compared to NPK0 treatment.The NUE in NPKM1,NPKM2,and NPKM3 treatments was higher as compared to NPK1 treatment for both rice seasons.Redundancy analysis revealed close positive relationships of CSR with C input,total N,soil C:N ratio,catalase,and humic acids,whereas NUE was closely related to grain yield,grain N content,and phenol oxidase.Furthermore,CSR and NUE negatively correlated with humin acid and soil C:P and N:P ratios.The technique for order of preference by similarity to ideal solution(TOPSIS)showed that NPKM3 treatment was the optimum strategy for improving CSR and NUE.Therefore,substituting 70%of chemical fertilizer with organic manure could be the best management option for increasing CSR and NUE in the paddy fields of southern China.

Production Benefits of Double-Cropping Rice Under Optimized Application of Nitrogen and Potassium Fertilizers Combined with Chinese Milk Vetch and Straw Co-Returning to Fields

In order to explore the technology and effects of reducing nitrogen and potassium fertilizer applications in double-cropping rice,a field plot experiment was conducted to study the effects of optimized application of nitrogen and potassium fertilizers combined with returning Chinese milk vetch and straw to fields on yield,fertilizer utilization efficiency,net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci),chlorophyll content(SPAD value)and soil physical and chemical properties in late rice harvest period.The results showed that the optimized application of nitrogen and potassium fertilizers combined with the integrated technology of Chinese milk vetch and straw co-returning to the field could enhance the photosynthetic efficiency of double-cropping rice,increase rice yield,and enhance soil biological activity,especially T4 treatment involving the returning of Chinese milk vetch and straw to the field instead of 30%nitrogen fertilizer achieved the highest rice yield,fertilizer use efficiency,net photosynthetic rate and soil biological activity.Compared with the conventional fertilization treatment T2,the total rice yield of T4 treatment increased by 4.1%,among which the early rice and late rice increased by 6.3%and 2.4%,respectively;Pn,Gs and SPAD values of flag leaves at full heading stage significantly increased,and the contents of soil active organic carbon,alkali hydrolyzed nitrogen,available phosphorus and readily available potassium significantly increased.

Effect of Reducing Application of Chemical Fertilizers and Chemical Pesticides on Physiological Traits,Yield and Quality of Radish

This paper studied the effect of reducing application of chemical fertilizer,increasing application of functional organic fertilizer and trace elements and fully using biological pesticides on physiological traits,yield and quality of radish. The results showed that applying functional organic fertilizer and trace elements and fully using biological pesticides could help to significantly improve the radish fleshy root yield,increase soluble sugar and vitamin C content,reduce crude fiber and nitrate content,but had no significant influence on radish moisture content,glucosinolates and chlorpyrifos content in organophosphorus pesticides.

Effects of Different Nitrogen Applications on Soil Physical, Chemical Properties and Yield in Maize (<i>Zea mays</i>L.)

Application of nitrogen (N) fertilizer is one of the most important approaches on improving maize grain yield. However, as is known to all, overuse N fertilizer not only leads to decline of N use efficiency and maize yield, but also leads to potential risk to environment pollution. This experiment was conducted to determine the effects of N fertilizer applications with nine different treatments on soil physical-chemical characters and maize grain yield using hybrid variety Zhengdan 958 in 2011 and 2012. Results indicated that the soil bulk densities of T2 (CK) and T1 were the lowest compared to other treatments in 2011 and 2012, respectively, whereas the soil bulk density of T5 in 2011 and T3 in 2012 were higher than other treatments. The soil porosity and field capacity of T5 in 2011 and T3 in 2012 were lower than other treatments, but those of CK in 2011 and T1 in 2012 were higher than other treatments. The pH values of T3 to T7 were lower than other treatments. These results indicated that the soil bulk densities were increased, whereas the soil porosity, field capacity and values pH were decreased by N application at different stages. N application could increase the N contents of leaf and stem, whereas less or excess N application should not significant improve maize yield. Although the soil organic matter and total N contents of T3 were the highest in both 2011 and 2012, the yield of T4 is the highest in both 2011 and 2012. The application amount, period and times of N fertilizer were important to maize yield.

Agronomic Performances of Manure Composts in Simplified Soilless Tomato Cultivation

Composts are recognised as an important source of nutrients for crops. The study aims to valorise agricultural by-products by composts made from broiler (A), laying hen (B) and bovine (C) manures in soilless tomato cultivation. Treatments consisted of these three composts and controls consisting of coconut fibres fed with a nutrient solution. The system is a randomised Fisher block with three replications. Each elementary plot consisted of nine tomato plants. Chemical parameters of the substrates and agronomic parameters of the plants were recorded from 14 to 49 days after transplanting (DAT). The pH stabilised at around 6.2 after varying from 7.1 to 8.0 in the composts. The high electrical conductivity (5.9 - 6.01 dS/m) was less than 1 dS/m at 49 DAT. Agromorphological parameters were close to the controls. Fruit necrosis was higher in the compost-based substrates (13.75% - 32.22%) than in the controls (<2%). Healthy fruit yields from the composts (38.7 - 48.7 t/ha) were high, although lower than those from the controls (49.9 - 57.4 t/ha). Fruit harvested from these substrates had a longer average shelf life (38.23 days) than the controls (28.5 days). This study showed that composts have fertilising properties for soilless tomato cultivation, in particular that of laying hen manure (48.33 t/ha). These composts could provide an alternative to the use of chemical fertilisers in soilless tomato cultivation.

Organic Amendments with Poultry Manure and Cow Dung Influence the Yield and Status of Nutrient Uptake in Wheat (Triticum aestivum)

The field experiment cultivating wheat during the winter season from November 2014 to March 2015 was conducted in the “North Eastern Barind Tract Soils” at Kushadaha, Nawabganj, Dinajpur in Bangladesh. The wheat variety BARI Gom 26 was selected in focusing to evaluate the effects of organic amendments using poultry manure (PM) and cow dung (CD) on the growth, yield and nutrient uptake by the plant. The texture of the field soil was clay with acidic nature (pH 5.61). The trials comprise three treatments following a randomized complete block design (RCBD) replicated thrice. Results showed that significantly greater plant height, number of tillers per hill and straw yield were 98.10 cm, 3.66 and 5425 kg/ha respectively in receiving the treatment T2 than T3 (95.66 cm, 3.38 and 4483 kg/ha) and T1 (control). Spike length, 1000 grain weight and grain yield were 9.23 cm, 39.81 kg/ha and 3100 kg/ha respectively also higher in T2 treatment than T3 (8.76 cm, 38.51 kg/ha, 3091 kg/ha) with no statistical differences among them. Therefore, the treatment T2 (PM) comprised of poultry manure and NPK exhibited as the best treatment for producing the highest in all growth and yield parameters of wheat. In wheat grain, the content of N, P, K, S, Mg, Zn and B ranged from 1.080% to 1.380% N, 0.390% to 0.398% P, 0.780% to 0.840% K, 0.079% to 0.111% S, 0.181% to 0.187% Mg, 25.56 to 29.77 ppm Zn and 10.12 to 12.54 ppm B. Similarly in straw, nutrients content ranged from 0.220 to 0.300% N, 0.045% to 0.074% P, 0.970% to 1.250% K, 0.152% to 0.191% S, 0.097% to 0.101% Mg, 10.78 to 13.23 ppm Zn and 27.98 to 2989 ppm B. Therefore, organic amendment with 5-ton cow dung (CD) ha-1 with a recommended dose of NPK significantly increased the content of N, S, Mg, Zn and B in the grain and straw of wheat. Alternatively, the poultry manure treatment with NPK significantly increased the content of P and K in the grain and straw of wheat. However, results revealed that the treatments T2 and T3 comprising a recommended dose of NPK with poultry manure and cow dung respectively could increase the content of N, P, K, S, Mg, Zn and B in wheat. The overall results expressed that the poultry manure treatment with NPK (T2) exposed as superior for producing the top growth and yield attributes of wheat in the studied area.

Effect of chemical and organic fertilization on soil carbon and nitrogen accumulation in a newly cultivated farmland

Increased food demand from the rapidly growing human population has caused intensive land transition from desert to farmland in arid regions of northwest China. In this developing ecosystem, the optimized fertilization strategies are becoming an urgent need for sustainable crop productivity, efficient resources use, together with the delivery of ecosystems services including soil carbon（C） and nitrogen（N） accumulation. Through a 7-year field experiment with 9 fertilization treatments in a newly cultivated farmland, we tested whether different fertilizations had significant influences on soil C and N accumulation in this developing ecosystem, and also investigated possible mechanisms for this influence. The results showed that applying organic manure in cultivated farmland significantly increased the soil C and N accumulation rates; this influence was greater when it was combined with chemical fertilizer, accumulating 2.01 t C and 0.11 t N ha^（–1） yr^（–1） in the most successful fertilization treatment. These high rates of C and N accumulation were found associated with increased input of C and N, although the relationship between the N accumulation rate and N input was not significant. The improved soil physical properties was observed under only organic manure and integrated fertilization treatments, and the significant relationship between soil C or N and soil physical properties were also found in this study. The results suggest that in newly cultivated farmland, long term organic manure and integrated fertilization can yield significant benefits for soil C and N accumulation, and deliver additional influence on physical properties.

Comparison of Different Types of Fertilizers on Growth, Yield and Quality Properties of Watermelon (<i>Citrllus lanatus</i>)

Background: Watermelon is a very important crop because it has many nutritional and economical values. This crop is known to human for ages and has been cultivated for centuries in many Middle Eastern countries including Syria. Several types of fertilizers are being used in order to increase the productivity of this crop but some fertilizers have shown to have an adverse effect on environment. Therefore, the aim of this study was to examine the effects of several types of organic fertilizers (cow, sheep, poultry and pigeon manure) and to compare their effects on growth, productivity and quality parameters with chemical fertilizers (NPK) and control group. Materials and Methods: Two local cultivars spherical and cylindrical (Audrey and Shapah) were used to examine the effects of cow manure (8 m/Donum), Sheep manure (6 m/Donum), poultry manure (3 m/Donum) and pigeons (2 m/Donum) and were compared with chemical fertilizer (N 20:P 40:K 25). Number of fruits on each vine, length of each vine (cm), fruit weight (kg), and estimated yield (kg/Donum) were measured and recorded some quality parameters such as rind thickness (cm) and heart color as well. Results: Cows manure was proved to be superior to other types of fertilizers (organic and inorganic) in many traits but chemical fertilizer gave the highest yield (kg/Donum) in Audrey cultivar but in Shapah cultivar there was no significant difference in productivity between chemical fertilizer compared with control group. Conclusion: Using organic fertilizers to cultivate watermelon does affect quality of watermelon and the productivity was closely related to chemical fertilizer. On the other hand, organic fertilizer can reduce the harmful effects of chemical fertilizers on environment and human health alike.

Mitigating greenhouse gas of chemical fertilizer with farmland emissions through replacement organic manure in a temperate

Burning crop residues and excessive use of chemical fertilizers results in an enormous waste of bio- logical resources, which further weakens the potential capacity of the agro-ecosystem as a carbon sink. To explore the potential of farmlands acting as a carbon sink without yield losses, we conducted an experiment on a temperate eco-farm in eastern rural China. Crop residues were applied to cattle feed, and the composted cattle manure was returned to cropland with a winter wheat and maize rotation. Four different proportions of fertilizers were designed： 100 % cattle manure, 100 % mineral nitrogen, 75 % cattle manure plus 25 % mineral nitrogen, and 50 % cattle manure plus 50 % mineral nitrogen. Crop yield and greenhouse gas （GHG） emissions were carefully calculated according to the Intergovernmental Panel on Climate Change （IPCC） Guidelines for National Green- house Gas Inventories 2006. Our results showed that replacing chemical fertilizer with organic manure signifi- cantly decreased the emission of GHGs. Yields of wheat and corn also increased as the soil fertility was improved by the application of cattle manure. Totally replacing chemical fertilizer with organic manure decreased GHG emissions, which reversed the agriculture ecosystem from a carbon source （＋2.7 t CO_2-eq. hm-2 year-1） to a carbon sink （-8.8 t CO_2-eq. hm^-2 year^-1）. Our findings provide useful insights for improving agricultural ecosystems under global change scenarios.

有机肥替代化肥氮对谷子氮素累积、产量及品质的影响

有机肥部分替代化肥是一种实现化肥减量的可持续农业生产措施。本研究于2020和2021连续2年以“沁黄2号”为供试材料,设不施氮(CK)、常量化肥氮(NPK)、有机肥替代25%化肥氮(25%M)、有机肥替代50%化肥氮(50%M)、有机肥替代75%化肥氮(75%M)和有机肥替代100%化肥氮(100%M) 6个处理,研究不同替代率对谷子产量构成和氮素吸收的影响,并分析氮素吸收调控小米米色、糊化特性和类胡萝卜素组分的效应,明确谷子生产中有机肥最佳替代率。结果表明,2年中较低的有机肥替代化肥氮率显著提高了谷子植株氮素累积,但随着替代率的持续增加,植株氮素累积量呈降低趋势,最终影响谷子产量和小米品质。2020年, 25%M处理显著提高了谷子地上部氮素累积量,较NPK处理提高9.6%;2021年,25%M处理谷子地上部氮素累积量、生物量、穗粒数和产量达到最高,较NPK处理分别提高6.1%、12.0%、15.4%和12.0%。50%M处理显著影响了小米的米色、糊化特征和类胡萝卜素含量,与NPK相比,小米籽粒红绿值、橘色值、支链淀粉含量、总淀粉含量、小米糊化最终黏度、叶黄素含量、玉米黄质含量和黄色素含量的增幅分别可达6.0%、6.0%、7.4%、4.3%、7.8%、20.7%、17.4%和2.8%。但有机肥完全替代化肥降低了谷子地上部氮素吸收、地上部生物量、穗粒数及谷子产量,也抑制了小米叶黄素和玉米黄质含量的提升。2年均表现出谷子地上部氮吸收量与小米单粒重、直链淀粉含量和小米糊化回升值呈显著的负相关。且2021年谷子地上部氮吸收量还与小米总淀粉含量、蛋白质含量、小米粉糊化峰值黏度和小米黄色素含量呈显著的负相关,与小米糊化峰谷黏度呈显著的正相关。综上,施氮总量120 kg hm^(–2)下,有机肥替代25%~50%化肥氮能通过促进植株氮素的吸收,实现产量、米色、蒸煮特性及类胡萝卜素的协同提升,为谷子化肥减量和提质增效生产提供技术支撑。

Effect of Long-Term Fertilization on Organic Carbon and Nitrogen in a Subtropical Paddy Soil

A long-term experiment beginning in 1981 in Jinxian County of Jiangxi Province, subtropical China, was conducted in a paddy field under a double rice cropping system with four different fertilization regimes, including 1) no fertilizer as control (CK), 2) balanced chemical N, P, and K fertilizers (NPK), 3) organic manure using milk vetch and pig manure in the early and late rice growing season, respectively (OM), and 4) balanced chemical fertilizers combined with organic manure (NPKM). Samples (0-17 cm) of the paddy field soil, which was derived from Quaternary red clay, were collected after the late rice harvest in November 2003 for determination of total organic carbon (TOC) and total nitrogen (TN) and fractions of organic C and N. Results showed that TOC and TN in the NPKM and OM treatments were significantly higher than those in other two treatments (CK and NPK). Application of organic manure with or without chemical fertilizers significantly increased the contents of all fractions of organic C and N, whereas chemical fertilizer application only increased the contents of occluded particulate organic C (oPOC) and amino acid N. In addition, application of organic manure significantly enhanced the proportions of free particulate organic carbon (fPOC) and oPOC in total C, and those of amino sugar N and amino acid N (P < 0.01) in total N. In contrast, chemical fertilizer application only increased the proportions of oPOC and amino acid N (P < 0.05). There were no significant differences in either contents or proportions of soil organic C and organic N fractions between the NPKM and OM treatments. These indicated that organic manure application with or without chemical fertilizers played the most significant role in enhancing soil organic C and N quantity and quality in the paddy field studied.

Effect of Long-Term Fertilization on Organic Nitrogen Forms in a Calcareous Alluvial Soil on the North China Plain

In order to illustrate the change of nitrogen （N） supply capacity after long-term application of manure and chemical fertilizer, as well as to properly manage soil fertility through fertilizer application under the soil-climatic conditions of the North China Plain, organic N forms were quantified in the topsoil with different manure and chemical fertilizer treatments in a 15-year fertilizer experiment in a Chinese calcareous alluvial soil. Soil total N （TN） and various organic N forms were significantly influenced by long-term application of chemical fertilizer and manure. TN, total hydrolysable N, acid-lnsoluble N, amino acid N and ammonium N in the soil increased significantly （P 〈 0.05） with increasing manure and fertilizer N rates, but were not influenced by increasing P rates. Also, application of manure or N fertilizer or P fertilizer did not significantly influence either the quantity of amino sugar N or its proportion of TN. Application of manure significantly increased （P 〈 0.05） hydrolysable unknown N, but adding N or P did not. In addition, application of manure or N fertilizer or P fertilizer did not significantly influence the proportions of different soil organic N forms.

Current Situation and Countermeasures of Utilization of Crop Straw as Feed in Sichuan Province

Utilizing straw as feed and applying cattle and sheep manure fertilizer to the field plays an important role in solving the shortage of feed raw material resources,alleviating the conflict between human beings and livestock,and realizing the full utilization of straw as a resource and green and low-carbon circular development.We carried out an investigation on the feed utilization of crop straw in Sichuan Province,comprehensively grasped the crop straw resources and its utilization as feed in this province,deeply analyzed the existing problems,and put forward countermeasures and suggestions according to the actual situation in Sichuan,which plays an important role in accelerating the process of straw feed utilization,promoting green development,implementing the rural revitalization strategy,and building a beautiful Sichuan.

Effects of Soil Moisture and Fertilization Treatments on the Agronomic Traits of Teff (<i>Eragrosis tef</i>) in Southern Kyushu, Japan

This study investigated the agronomic characteristics of pot-cultured teff (Eragrostis tef) under soil moisture and fertilization treatments in southern Kyushu, Japan. In experiments examining the effects of soil moisture and fertilization, growth of teff and Setaria (Setaria sphacelata) as a reference species was compared under six treatments combining two soil moisture regimes, control (C) and high moisture (H) treatment, and three fertilizer applications, chemical fertilizer with manure (CF + M), chemical fertilizer (CF) and doubled level of manure (DM). H treatment did not decrease dry matter yield in teff two months after transplanting, except when combined with CF + M, compared with a decreasing tendency in Setaria. Top and underground dry matter yields and harvest index in teff increased significantly (P -2);6.2 gN m-2 is the standard level in the USA. Dry matter yield in teff did not differ significantly (P > 0.05) at CF application levels above 6.2 gN m-2 two months after sowing, suggesting that the optimum CF level of teff in southern Kyushu was comparable with that in the USA, and that teff had potential as an early-harvest forage crop two months after planting to replace summer forage in paddy fields of this region.

有机肥与化肥配施对夏播玉米产量和土壤肥力的影响

为探索有机肥与化肥配施对全株玉米产量和土壤肥力的影响,以乐玉1号玉米为试材,通过田间试验,设置不施肥(CK)、全部化肥(HF)、牛粪单施(N1)、牛粪替代20%化肥(N2)、牛粪替代30%化肥(N3)、羊粪单施(Y1)、羊粪替代20%化肥(Y2)、羊粪替代30%化肥(Y3)共8个处理,测定全株玉米干草产量、氮磷钾素积累量和重金属含量以及土壤理化性质等相关指标。结果表明,Y2和Y3处理的全株玉米干草产量和氮磷钾积累量优于其他处理;N2处理的Cu积累量和Y1处理的Zn积累量最高;0~20cm土层以N3处理的有机质、速效磷、铵态氮、硝态氮和阳离子交换含量最高。可见,低总氮水平下,羊粪替代20%和30%化肥处理对全株玉米生长及养分积累有积极作用,牛粪替代30%化肥改良土壤肥力效果较好。

连续配施有机肥对茶园土壤细菌群落结构及功能类群的影响

为评估有机肥配施化肥对茶园土壤细菌群落的影响,于田间试验条件下选取了连续6年不施肥(CK)、单施化肥(CF)、单施化肥+清园处理(CFC)、有机肥配施化肥(OF)和有机肥配施化肥+种植绿肥(OFM)5个处理,采用Illumina MiSeq高通量测序技术,分析了不同施肥措施对酸性茶园土壤细菌群落多样性、群落结构、功能类群及共现性网络结构的影响。结果表明:与CK处理相比,CF处理降低了土壤pH,但显著增加了土壤碱解氮和全磷含量,而OF和OFM处理在提高土壤pH的同时增加了土壤速效养分和有机质含量。CF和CFC处理降低土壤细菌群落Alpha多样性指数,OFM处理显著增加了土壤细菌Sobs、Ace、Chao1和Shannon指数(P<0.05)。非度量多维尺度分析(NMDS分析)及相似性分析结果显示,不同施肥措施显著改变了茶园土壤细菌群落结构(R^(2)=0.4421,P=0.001),OF处理富集了有益于土壤碳氮循环和抑制病原菌的物种。FAPROTAX细菌功能预测表明,茶园土壤细菌生态功能类群以化能异养、有氧化能异养、固氮作用、纤维素分解和硝酸盐还原作用功能类群为主,OF处理提高了捕食性或外寄生作用、光异养作用、光养作用、光合自养硫氧化、光能自养功能及氮循环功能细菌种群丰度。施肥措施均增加了细菌共现性网络边数、平均连通度和平均路径长度,细菌网络的规模增大,细菌群落间互相作用更复杂。细菌网络均以正相关为主(比例为58.03%~77.78%),施肥后物种间竞争作用进一步加强。施肥处理下细菌分子网络平均路径距离较长,平均聚类系数降低、细菌群落作用的响应速度变慢、群落结构稳定性提高,其中有机肥配施化肥处理细菌群落结构稳定性要优于CF处理。冗余分析和Mantel test分析结果表明,速效磷和pH是影响土壤细菌群落丰度和多样性的主要影响因子。总体而言,有机肥配施化肥改变了茶园土壤细菌群落结构,促进了土壤细菌碳氮循环功能,提高了细菌网络规模和群落互作,有机肥替代使细菌群落网络结构更加稳定,有利于构建一个健康稳定的土壤细菌群落。

Effects of Fertilizing Materials on Rapidly Available-N in Black Soil in Songnen Plain

The analyses on the mathematical models of the long- term location test in black soil in Song-nen Plain shows that farm manure and chemical fertilizers make great contribution to rapidly available-N in soil, while stalk makes less contribution. The most optimal pattern of fertilizing materials is 28 t / ha of farm manure, 630 kg / ha chemical fertilizers and 2.41 / ha of stalk.