In order to study the physiological or pathophysiological responsiveness, airway smooth muscle cells were isolated from the rat trachea and cultured. The tracheas of adult rats were obtained by autopsy within 30 minut...In order to study the physiological or pathophysiological responsiveness, airway smooth muscle cells were isolated from the rat trachea and cultured. The tracheas of adult rats were obtained by autopsy within 30 minutes after death and placed into a modified Hank’s solution. The experiments were performed on the large branches of the rat bronchi following entry into the lung. At the terminal level of the airway, no cartilage segments were seen. The muscle layer was prepared by careful removal of the connective tissue, cut into small pieces (1 mm3), and dispersed enzymatically (collagenase, elastase) into single cells. Freshly isolated cells could be used the same day. The small muscle pieces and isolated cells were cultured in the modified Hank’s or modified Eagle’s medium with 5% CO2 at 37℃ .展开更多
文摘In order to study the physiological or pathophysiological responsiveness, airway smooth muscle cells were isolated from the rat trachea and cultured. The tracheas of adult rats were obtained by autopsy within 30 minutes after death and placed into a modified Hank’s solution. The experiments were performed on the large branches of the rat bronchi following entry into the lung. At the terminal level of the airway, no cartilage segments were seen. The muscle layer was prepared by careful removal of the connective tissue, cut into small pieces (1 mm3), and dispersed enzymatically (collagenase, elastase) into single cells. Freshly isolated cells could be used the same day. The small muscle pieces and isolated cells were cultured in the modified Hank’s or modified Eagle’s medium with 5% CO2 at 37℃ .
