Objective To investigate the expression of aquaporin-1 (AQP1) in the human peritoneum and to evaluate the effect of peritoneal dialysis (PD) on its expression.Methods Peritoneal biopsies were obtained from normal subj...Objective To investigate the expression of aquaporin-1 (AQP1) in the human peritoneum and to evaluate the effect of peritoneal dialysis (PD) on its expression.Methods Peritoneal biopsies were obtained from normal subjects (n =10), uremic nondialysis patients (n = 12) at catheter insertion and PD patients ( n = 10) at the time of catheter removal, reinsertion or renal transplantation. Western blot, immuno-histochemical staining and reverse transcript-polymerase chain reaction ( RT-PCR) techniques were used to investigate AQP1 expression.Results All peritoneal samples expressed AQP1 at both mRNA and protein levels. Western blot revealed a major band at 28 kD as well as more diffuse bands between 35 and 50 kD. The 28 kD band represents the nonglycosylated form of the protein while the 35 - 50 kD bands correspond to glycosylated AQP1. Immunohistochemical staining found the positive deposits were distributed in the mesothelial cells, endothelial cells of capillaries, venules and small veins, whereas no signal was detected in the arterioles. Semi-quantitative analysis showed that AQP1 expression was remarkably stable in all samples, whatever their origin (P>0. 05).Conclusions Our findings suggested that AQP1 is the molecular counterpart of an ultra small pore during PD. Secondly, the peritoneal mesothelial cell might also be involved in peritoneal transcellular water transport. As regards whether or not the structural or distributional alterations of AQP1 in the peritoneum may be more obviously expressed during PD, further study is needed.展开更多
目的探究黄芪注射液对含糖乳酸盐腹膜透析液所致水孔蛋白1表达异常的影响。方法选取70只SD雄性大鼠,分成7组,分别注射不同浓度的黄芪注射液及乳酸盐腹膜透析液,留取引流液及提取腹膜样本进行有关AQP1表达及AQP1 m RNA检测。结果高糖高...目的探究黄芪注射液对含糖乳酸盐腹膜透析液所致水孔蛋白1表达异常的影响。方法选取70只SD雄性大鼠,分成7组,分别注射不同浓度的黄芪注射液及乳酸盐腹膜透析液,留取引流液及提取腹膜样本进行有关AQP1表达及AQP1 m RNA检测。结果高糖高黄芪组、高糖组BUN清除率低于对照组,差异有统计学意义(P<0.05)。高糖高黄芪组AQP1表达(11.01±1.76)低于对照组(13.12±2.02),差异有统计学意义(P<0.05);低糖高黄芪组AQP1 m RNA表达高于低糖组与高糖低黄芪组,差异有统计学意义(P<0.05)。结论适量浓度的黄芪注射液对AQP1表达异常影响不明确。展开更多
基金This study was supported by the National Natural Science Foundation of China (No.39870903) and Shanghai Education Grant (No.98QN65).
文摘Objective To investigate the expression of aquaporin-1 (AQP1) in the human peritoneum and to evaluate the effect of peritoneal dialysis (PD) on its expression.Methods Peritoneal biopsies were obtained from normal subjects (n =10), uremic nondialysis patients (n = 12) at catheter insertion and PD patients ( n = 10) at the time of catheter removal, reinsertion or renal transplantation. Western blot, immuno-histochemical staining and reverse transcript-polymerase chain reaction ( RT-PCR) techniques were used to investigate AQP1 expression.Results All peritoneal samples expressed AQP1 at both mRNA and protein levels. Western blot revealed a major band at 28 kD as well as more diffuse bands between 35 and 50 kD. The 28 kD band represents the nonglycosylated form of the protein while the 35 - 50 kD bands correspond to glycosylated AQP1. Immunohistochemical staining found the positive deposits were distributed in the mesothelial cells, endothelial cells of capillaries, venules and small veins, whereas no signal was detected in the arterioles. Semi-quantitative analysis showed that AQP1 expression was remarkably stable in all samples, whatever their origin (P>0. 05).Conclusions Our findings suggested that AQP1 is the molecular counterpart of an ultra small pore during PD. Secondly, the peritoneal mesothelial cell might also be involved in peritoneal transcellular water transport. As regards whether or not the structural or distributional alterations of AQP1 in the peritoneum may be more obviously expressed during PD, further study is needed.
文摘目的探究黄芪注射液对含糖乳酸盐腹膜透析液所致水孔蛋白1表达异常的影响。方法选取70只SD雄性大鼠,分成7组,分别注射不同浓度的黄芪注射液及乳酸盐腹膜透析液,留取引流液及提取腹膜样本进行有关AQP1表达及AQP1 m RNA检测。结果高糖高黄芪组、高糖组BUN清除率低于对照组,差异有统计学意义(P<0.05)。高糖高黄芪组AQP1表达(11.01±1.76)低于对照组(13.12±2.02),差异有统计学意义(P<0.05);低糖高黄芪组AQP1 m RNA表达高于低糖组与高糖低黄芪组,差异有统计学意义(P<0.05)。结论适量浓度的黄芪注射液对AQP1表达异常影响不明确。
