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Performance Parameters:Demobilization Antibiotic Resistant Bacteria(ARB)and Carrying Genes(ARG)in Wastewater Disinfection
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作者 Solange Kazue Utimura Denise Crocce Romano Espinosa +2 位作者 Marcio Luís Busi da Silva Elisabete de Santis Braga Pedro Jose Alvarez 《Journal of Environmental Science and Engineering(B)》 2024年第1期1-8,共8页
The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacteri... The UV irradiation is used for removing Antibiotic Resistant Bacteria(ARB)and Antibiotic Resistance Genes(ARG)from wastewater treatment.Bacteriophages are viruses that infect within bacteria,are recognized for bacterial control.The influence of some parameters in quantification and performance influencing of pathogen demobilization could be considered in disinfection of wastewater.The comparison of Polyvalent phage(NE1)versus Coliphage(NE4)in suppressing a bacterium Escherichia coli(NDM-1:b-lactam-resistant)with UV irradiation was observed the efficacy in reduction of cells in the disinfection and parameter process.The results with the effect of UV-C irradiation on NDM-1 infected with 1%of NE4 showed a decrease of cells from 8×10^(6)to 2×10^(5)in 60 min with UV-C dose.The NDM1(E.coli)was infected with 1%of NE4(Polyvalent Phage)under magnetic stirring for 1 h,the cells count was 8×10^(6).After 1 h in UV-C e×posure,the cells number reached 3×10^(5).The NDM1 that was e×posed in 1 h of UV-C irradiation and then was infected with 1%of NE4.Cells counting were done 24 h after this procedure.These cells were e×posed in UV-C and showed a reduction in the number of cells from 1×10^(8)to 4×10^(5)after 60 min.The results indicate that bacteriophages can mitigate bacteria species,and combined the conventional water disinfection technologies that can support the microbial safety control strategies. 展开更多
关键词 Antibiotic Resistant Bacteria(ARB) Antibiotic Resistance genes(ARG) wastewater treatment DISINFECTION Escherichia coli(E.coli).
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Impact of wastewater treatment plant effluent discharge on the antibiotic resistome in downstream aquatic environments:a mini review 被引量:1
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作者 Zhiguo Su Lyujun Chen Donghui Wen 《Frontiers of Environmental Science & Engineering》 SCIE EI CSCD 2024年第3期131-141,共11页
Antimicrobial resistance(AMR)has emerged as a significant challenge in human health.Wastewater treatment plants(WWTPs),acting as a link between human activities and the environment,create ideal conditions for the sele... Antimicrobial resistance(AMR)has emerged as a significant challenge in human health.Wastewater treatment plants(WWTPs),acting as a link between human activities and the environment,create ideal conditions for the selection and spread of antibiotic resistance genes(ARGs)and antibioticresistant bacteria(ARB).Unfortunately,current treatment processes are ineffective in removing ARGs,resulting in the release of large quantities of ARB and ARGs into the aquatic environment through WWTP effluents.This,in turn,leads to their dispersion and potential transmission to human through water and the food chain.To safeguard human and environmental health,it is crucial to comprehend the mechanisms by which WWTP effluent discharge influences the distribution and diffusion of ARGs in downstream waterbodies.In this study,we examine the latest researches on the antibiotic resistome in various waterbodies that have been exposed to WWTP effluent,highlighting the key influencing mechanisms.Furthermore,recommendations for future research and management strategies to control the dissemination of ARGs from WWTPs to the environment are provided,with the aim to achieve the“One Health”objective. 展开更多
关键词 Antibiotic resistance genes(ARGs) Wastewater treatment plants(WWTPs) Effluent-receiving waterbodies Environmental risk Influencing mechanism
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Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence factors in receiving rivers:benefits of wastewater treatment plant upgrade 被引量:5
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作者 Guannan Mao Donglin Wang +1 位作者 Yaohui Bai Jiuhui Qu 《Frontiers of Environmental Science & Engineering》 SCIE EI CSCD 2023年第7期47-55,共9页
Wastewater treatment plants(WWTPs)with additional tertiary ultrafiltration membranes and ozonation treatment can improve water quality in receiving rivers.However,the impacts of WWTP upgrade(WWTP-UP)on pathogens carry... Wastewater treatment plants(WWTPs)with additional tertiary ultrafiltration membranes and ozonation treatment can improve water quality in receiving rivers.However,the impacts of WWTP upgrade(WWTP-UP)on pathogens carrying antibiotic resistance genes(ARGs)and virulence factors(VFs)in rivers remain poorly understood.In this study,ARGs,VFs,and their pathogenic hosts were investigated in three rivers impacted by large-scale WWTP-UP.A five-year sampling campaign covered the periods before and after WWTP-UP.Results showed that the abundance of total metagenome-assembled genomes(MAGs)containing both ARGs and VFs in receiving rivers did not decrease substantially after WWTP-UP,but the abundance of MAGs belonging to pathogenic genera that contain both ARGs and VFs(abbreviated as PAVs)declined markedly.'Genome-resolved metagenomics further revealed that WWTP-UP not only reduced most types of VFs and ARGs in PAVs,but also effectively eliminated efflux pump and nutritional VFs carried by PAVs in receiving rivers.WWTP-UP narrowed the pathogenic host ranges of ARGs and VFs and mitigated the cooccurrence of ARGs and VFs in receiving rivers.These findings underline the importance of WWTPUP for the alleviation of pathogens containing both ARGs and VFs in receiving rivers。 展开更多
关键词 Wastewater treatment plant upgrade Antibiotic resistance genes(ARGs) Virulencefactors(VFs) geneco-occurrence Genome-centric analysis
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Enzymatic pretreatment mitigates the dissemination of antibiotic resistance genes via regulating microbial populations and gene expressions during food waste fermentation 被引量:1
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作者 Yang Wu Wanying Hu +3 位作者 Haining Huang Xiong Zheng Lei Dong Yinguang Chen 《Chinese Chemical Letters》 SCIE CAS CSCD 2023年第8期259-264,共6页
Food waste(FW)has been recognized as essential reservoir for resource recovery via anaerobic fermentation,which could also bring the potential risk of antibiotic resistance genes(ARGs)dissemination.Although the struct... Food waste(FW)has been recognized as essential reservoir for resource recovery via anaerobic fermentation,which could also bring the potential risk of antibiotic resistance genes(ARGs)dissemination.Although the structural deficiency of FW could be stimulated by enzymatic pretreatment to enhance fermentation efficiency,the influences of enzymatic pretreatment on ARGs fate and microbial metabolic pathways involved in ARGs dissemination have rarely been reported.This work proved that enzymatic pretreatment could effectively decrease the total abundance of ARGs(reduced by 13.8%-24.5%)during long-term FW fermentation.It was found that enzymatic pretreatment significantly reduced the ARGs belonging to the efflux pump,which might be ascribed to its ability to increase membrane permeability.Furthermore,enzymatic pretreatment was in favor of reducing microbial diversity and various potential ARGs host(e.g.,Methanosarcina,Clostridium,Prevotella,Parabacteroides).Also,this pretreatment remarkably up-regulated the genetic expressions involved in ABC transporter(e.g.,eryF and mntA)and down-regulated the genetic expressions that participated in DNA replication,two-component systems(e.g.,uphA and cckA),and quorum sensing(e.g.,rpfF and lsrG),thereby decreasing ARGs transmission.This study would expand the insight of the influences of pretreatment method on ARGs fate during FW fermentation,and offer practical guidance on the sustainable management of FW. 展开更多
关键词 Food waste(FW) Antibiotic resistance genes(ARGs) Enzymatic pretreatment Microbial populations Metabolic pathways
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Plant cultivar determined bacterial community and potential risk of antibiotic resistance gene spread in the phyllosphere 被引量:1
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作者 Xiaoting Fan Jianqiang Su +2 位作者 Shuyidan Zhou Xinli An Hu Li 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第5期508-518,共11页
The global increased antibiotic resistance level in pathogenic microbes has posed a significant threat to human health.Fresh vegetables have been recognized to be an important vehicle of antibiotic resistance genes(AR... The global increased antibiotic resistance level in pathogenic microbes has posed a significant threat to human health.Fresh vegetables have been recognized to be an important vehicle of antibiotic resistance genes(ARGs)from environments to human beings.Phyllosphere ARGs have been indicated to be changed with plant species,yet the influence of plant cultivar on the phyllospheric resistome is still unclear.Here,we detected the ARGs and bacterial communities in the phyllosphere of two cultivars of cilantros and their corresponding soils using high-throughput quantitative PCR technique and bacterial 16S rRNA gene-based high-throughput sequencing,respectively.We further identified the potential bacterial pathogens and analyzed the effects of plant cultivar on ARGs,mobile genetic elements(MGEs),microbiome and potential bacterial pathogens.The results showed that the cultivars did not affect the ARG abundance and composition,but significantly shaped the abundance of MGEs and the composition structure of bacteria in the phyllosphere.The relative abundance of potential bacterial pathogenswas significantly higher in the phyllosphere than that in soils.Mantel test showed that the ARG patterns were significantly correlated to the patterns of potential bacterial pathogens.Our results suggested that the horizontal gene transfer of ARGs in the phyllosphere might be different between the two cultivars of cilantro and highlighted the higher risk of phyllospheric microorganisms compared with those in soils.These findings extend our knowledge on the vegetable microbiomes,ARGs,and potential pathogens,suggesting more agricultural and hygiene protocols are needed to control the risk of foodborne ARGs. 展开更多
关键词 Cilantro Antibiotic resistance genes(ARGs) Horizontal gene transfer(HGT) Mobile genetic elements(MGEs) Pathogenic bacteria
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Effects of antibiotics on corncob supported solid-phase denitrification: Denitrification and antibiotics removal performance, mechanism, and antibiotic resistance genes 被引量:1
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作者 Yanjie Zhang Weiyang Dong +5 位作者 Congyu Li Haiyan Wang Huan Wang Yu Ling Guokai Yan Yang Chang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第8期24-36,共13页
Solid-phase denitrification(SPD)has been used in wastewater treatment plant effluent to enhance nitrate removal,and antibiotics co-existing in the effluent is a common environmental problem.In this study,it was system... Solid-phase denitrification(SPD)has been used in wastewater treatment plant effluent to enhance nitrate removal,and antibiotics co-existing in the effluent is a common environmental problem.In this study,it was systematically investigated the effect of single trace sulfamethoxazole(SMX)/trimethoprim(TMP)and theirmixture on microbial denitrification performance,the antibiotics removal,and antibiotics resistance genes(ARGs)in corncob supported SPD system.The average denitrification rate was improved by 46.90%or 61.09%with single 50μg/L SMX or TMP,while there was no significant inhibition with mixed SMX and TMP.The abundance of dominant denitrifiers(Comamonadaceae family and Azospia)and fermentation bacteria(Ancalomicrobium)were consistent with the denitrification performance of different antibiotics groups.Single SMX and TMP achieved relatively higher denitrification gene and enzyme abundance.Mixed SMX and TMP improved the denitrification gene copies,but they reduced the key denitrification enzymes except for EC 1.7.7.2.Additionally,the removal efficiency of TMP(56.70%±3.18%)was higher than that of SMX(25.44%±2.62%)in single antibiotic group,and the existence of other antibiotics(i.e.SMX or TMP)had no significant impact on the TMP or SMX removal performance.Biodegradation was the main removal mechanism of SMX and TMP,while sludge and corncob adsorption contributed a little to their removal.SMX had the risk of sulfanilamide resistance genes(SRGs)dissemination.Furthermore,network analysis indicated that Niveibacterium and Bradyrhizobium were the potential hosts of SRGs,which promoted the horizontal transmission of ARGs. 展开更多
关键词 Solid-phase denitrification(SPD) SULFAMETHOXAZOLE (SMX)/trimethoprim(TMP) Microbial community Denitrification genes Antibiotics resistance genes(ARGs)
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Effects of combined antibiotics on nitrification, bacteria and antibiotic resistance genes in activated sludge: Insights from legacy effect of antibiotics
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作者 Zhong-Xing Zhang Xiao-Yan Fan +2 位作者 Xing Li Yu-Xi Gao Jun-Ru Zhao 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第9期96-110,共15页
The effect of combined antibiotics exposure on nitrogen removal,microbial community assembly and proliferation of antibiotics resistance genes(ARGs)is a hotspot in activated sludge system.However,it is unclear that ho... The effect of combined antibiotics exposure on nitrogen removal,microbial community assembly and proliferation of antibiotics resistance genes(ARGs)is a hotspot in activated sludge system.However,it is unclear that how the historical antibiotic stress affects the subsequent responses of microbes and ARGs to combined antibiotics.In this study,the effects of combined sulfamethoxazole(SMX)and trimethoprim(TMP)pollution on activated sludge under legacy of SMX or TMP stress with different doses(0.005-30 mg/L)were investigated to clarify antibiotic legacy effects.Nitrification activity was inhibited under higher level of combined exposure but a high total nitrogen removal(∼70%)occurred.Based on the full-scale classification,the legacy effect of past antibiotic stress had a marked effect on community composition of conditionally abundant taxa(CAT)and conditionally rare or abundant taxa(CRAT).Rare taxa(RT)were the keystone taxa in the microbial network,and the responses of hub genera were also affected by the legacy of antibiotic stress.Nitrifying bacteria and genes were inhibited by the antibiotics and aerobic denitrifying bacteria(Pseudomonas,Thaurea and Hydrogenophaga)were enriched under legacy of high dose,as were the key denitrifying genes(napA,nirK and norB).Furthermore,the occurrences and co-selection relationship of 94 ARGs were affected by legacy effect.While,some shared hosts(eg.,Citrobacter)and hub ARGs(eg.,mdtD,mdtE and acrD)were identified.Overall,antibiotic legacy could affect responses of activated sludge to combined antibiotic and the legacy effect was stronger at higher exposure levels. 展开更多
关键词 ANTIBIOTICS Antibiotic resistance genes(ARGs) Combined pollution Full-scale classification Legacy effect
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Coastal mudflats as reservoirs of extracellular antibiotic resistance genes: Studies in Eastern China
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作者 Qingbin Yuan Xiaolin Wang +3 位作者 Hui Fang Yuan Cheng Ruonan Sun Yi Luo 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第7期58-68,共11页
Despite coastal mudflats serving as essential ecological zones interconnecting terrestrial/freshwater and marine systems,little is known about the profiles of antibiotic resistance genes(ARGs)in this area.In this stud... Despite coastal mudflats serving as essential ecological zones interconnecting terrestrial/freshwater and marine systems,little is known about the profiles of antibiotic resistance genes(ARGs)in this area.In this study,characteristics of typical ARGs,involving both intracellular(iARGs)and extracellular ARGs(eARGs)at different physical states,were explored in over 1000 km of coastal mudflats in Eastern China.Results indicated the presence of iARGs and eARGs at states of both freely present or attached by particles.The abundance of eARGs was significantly higher than that of iARGs(87.3%vs 12.7%),and their dominance was more significant than those in other habitats(52.7%-76.3%).ARG abundance,especially for eARGs,showed an increasing trend(p<0.05)from southern(Nantong)to northern(Lianyungang)coastalmudflats.Higher salinity facilitated the transformation from iARGs to eARGs,and smaller soil particle size was conducive to the persistence of eARGs in northern coastal mudflats.This study addresses the neglected function of coastal mudflats as eARGs reservoirs. 展开更多
关键词 Antibiotic resistance genes(ARGs) Extracellular antibiotic resistance genes(eARGs) Coastal mudflats Amplicon sequencing Geochemical variable Mobile genetic elements
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Association between Arg16Gly Polymorphism in the <i>Β<sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"></i>-Adrenergic Receptor Gene and Obesity Risk in Patients with Bronchial Asthma
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作者 Vladyslava V. Kachkovska Viktor F. Orlovskyi 《Open Journal of Genetics》 2021年第4期93-101,共9页
<b><span style="font-family:Verdana;">The objective of our study</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;&qu... <b><span style="font-family:Verdana;">The objective of our study</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> was to investigate the association between the Arg16Gly polymorphism in the </span><i><span style="font-family:Verdana;">β</span></i><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">-АR gene and body mass index (BMI) in patients with bronchial asthma (BA) with regard to the age of onset. </span><b><span style="font-family:Verdana;">Materials and methods</span></b></span><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> 553 patients were examined. The control group consisted of 95 apparently healthy individuals. The patients were divided into 2 groups depending on the age of BA onset: 282 patients with late onset (Group I) and 271 patients with early onset (Group II). Arg16Gly polymorphism in the </span><i><span style="font-family:Verdana;">β</span></i><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">-АR gene (rs1042713) was determined using polymerase chain reaction-</span></span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span style="font-family:Verdana;">restriction fragment length polymorphism analysis. Statistical analysis of obtained results was performed using SPSS–17 program. </span><b><span style="font-family:Verdana;">Results</span></b></span><b><span style="font-family:Verdana;">:</span><span style="font-family:;" "=""> </span></b><span style="font-family:;" "=""><span style="font-family:Verdana;">There was no significant difference in the distribution of genotypes for Arg16Gly polymorphism in the </span><i><span style="font-family:Verdana;">β</span></i><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">-AR gene depending on BMI (</span><i><span style="font-family:Verdana;">χ</span></i><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 5.74;р = 0.22), and no association was found with obesity risks disregarding the age of BA onset. The study on the frequency of genotypes for this polymorphism with regard to the age of asthma onset and BMI found statistically significant differences between early-onset (</span><i><span style="font-family:Verdana;">χ</span></i><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 11.27;p = 0.02) and late-onset (</span><i><span style="font-family:Verdana;">χ</span></i><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 10.66;p = 0.03) asthma. It was found that the risk of obesity in Group I patients was 1.57 times higher in the additive model and 2.67 times higher—in the recessive model of inheritance. In patients with late-onset asthma, Gly16 allele in the recessive model of inheritance was found to have a protective role against obesity. </span><b><span style="font-family:Verdana;">Conclusions</span></b></span><b><span style="font-family:Verdana;">:</span></b><span style="font-family:;" "=""><span style="font-family:Verdana;"> There was no significant difference in the distribution of genotypes for Arg16Gly polymorphism in the </span><i><span style="font-family:Verdana;">β</span></i><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">-AR gene with regard to BMI, and no association was found with obesity risks in BA patients disregarding the age of BA onset. The risk of obesity in patients with early-onset asthma was 1.6 times higher in the additive model and 2.7 times higher—in the recessive model of inheritance.</span></span> 展开更多
关键词 Bronchial Asthma Onset OBESITY Arg16Gly Polymorphism in the β2-Adrenoceptor gene
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Effective removal of antibiotic resistance genes and potential links with archaeal communities during vacuum-type composting and positive-pressure composting 被引量:5
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作者 Haonan Fan Shanghua Wu +5 位作者 John Woodley Guoqiang Zhuang Zhihui Bai Shengjun Xu Xuan Wang Xuliang Zhuang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2020年第3期277-286,共10页
As a major reservoir of antibiotics,animal manure contributes a lot to the augmented environmental pressure of antibiotic resistance genes(ARGs).This might be the first study to explore the effects of different ventil... As a major reservoir of antibiotics,animal manure contributes a lot to the augmented environmental pressure of antibiotic resistance genes(ARGs).This might be the first study to explore the effects of different ventilation types on the control of ARGs and to identify the relationships between archaeal communities and ARGs during the composting of dairy manure.Several ARGs were quantified via Real-time qPCR and microbial communities including bacteria and archaea were analyzed by High-throughput sequencing during vacuum-type composting(VTC) and positive-pressure composting(PPC).The total detected ARGs and class I integrase gene(intI1) under VTC were significantly lower than that under PPC during each stage of the composting(p <0.001).The relative abundance of potential human pathogenic bacteria(HPB) which were identified based on sequencing information and correlation analysis decreased by 74.6% and 91.4% at the end of PPC and VTC,respectively.The composition of archaeal communities indicated that methane-producing archaea including Methanobrevibacter,Methanocorpusculum and Methanosphaera were dominant throughout the composting.Redundancy analysis suggested that Methanobrevibacter and Methanocorpusculum were positively correlated with all of the detected ARGs.Network analysis determined that the possible hosts of ARGs were different under VTC and PPC,and provided new sights about potential links between archaea and ARGs.Our results showed better performance of VTC in reducing ARGs and potential HPB and demonstrated that some archaea could also be influential hosts of ARGs,and caution the risks of archaea carrying ARGs. 展开更多
关键词 Antibiotic resistance genes(ARGs) COMPOSTING ARCHAEA Human PATHOGENIC bacteria BACTERIAL COMMUNITIES
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Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system 被引量:5
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作者 Lingwei Meng Xiangkun Li +3 位作者 Xinran Wang Kaili Ma Gaige Liu Jie Zhang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2017年第11期110-117,共8页
This study aimed to reveal how amoxicillin(AMX) affected the microbial community and the spread mechanism of antibiotic resistance genes(ARGs) in the AMX manufacture wastewater treatment system. For this purpose, ... This study aimed to reveal how amoxicillin(AMX) affected the microbial community and the spread mechanism of antibiotic resistance genes(ARGs) in the AMX manufacture wastewater treatment system. For this purpose, a 1.47 L expanded granular sludge bed(EGSB) reactor was designed and run for 241 days treating artificial AMX manufacture wastewater. 454 pyrosequencing was applied to analyze functional microorganisms in the system. The antibiotic genes OXA_(-1), OXA_(-2), OXA_(-10), TEM_(-1), CTX-M_(-1), class I integrons(intI1) and 16 SrRNA genes were also examined in sludge samples. The results showed that the genera Ignavibacterium, Phocoenobacter,Spirochaeta, Aminobacterium and Cloacibacillus contributed to the degradation of different organic compounds(such as various sugars and amines). And the relative quantification of eachβ-lactam resistance gene in the study was changed with the increasing of AMX concentration.Furthermore the vertical gene transfer was the main driver for the spread of ARGs rather than horizontal transfer pathways in the system. 展开更多
关键词 Amoxicillin(AMX) Expanded granular sludge bed(EGSB) 454 pyrosequencing Antibiotic resistance genes(ARGs)
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Mechanistic insights for efficient inactivation of antibiotic resistance genes: a synergistic interfacial adsorption and photocatalytic-oxidation process 被引量:1
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作者 Zhiruo Zhou Zhurui Shen +5 位作者 Zhihui Cheng Guan Zhang Mingmei Li Yi Li Sihui Zhan John C.Crittenden 《Science Bulletin》 SCIE EI CSCD 2020年第24期2107-2119,M0006,共14页
Advanced oxidation processes(AOPs) have been applied to address multiple environmental concerns including antibiotic resistance genes(ARGs). ARGs have shown an increasing threat to human health,and they are either har... Advanced oxidation processes(AOPs) have been applied to address multiple environmental concerns including antibiotic resistance genes(ARGs). ARGs have shown an increasing threat to human health,and they are either harbored by antibiotic-resistant bacteria(ARB) or free in the environment.However, the control of ARGs has been substantially limited by their low concentration and the limited knowledge about their interfacial behavior. Herein, a novel AOP catalyst, Ag/TiO_(2)/graphene oxide(GO),combined with a polyvinylidene fluoride(PVDF) ultrafiltration membrane was designed with a synergistic interfacial adsorption and oxidation function to inactivate ARGs with high efficiency in both model solutions and in secondary wastewater effluent, especially when the residue concentration was low.Further analysis showed that the mineralization of bases and phosphodiesters mainly caused the inactivation of ARGs. Moreover, the interfacial adsorption and oxidation processes of ARGs were studied at the molecular level. The results showed that GO was rich in sp^(2) backbones and functional oxygen groups,which efficiently captured and enriched the ARGs via p-p interactions and hydrogen bonds. Therefore,the photogenerated active oxygen species attack the ARGs by partially overcoming the kinetic problems in this process. The Ag/Ti O2/GO catalyst was further combined with a PVDF membrane to test its potential in wastewater treatment applications. This work offers an efficient method and a corresponding material for the inactivation and mineralization of intra/extracellular ARGs. Moreover, the molecularlevel understanding of ARG behaviors on a solid–liquid interface will inspire further control strategies of ARGs in the future. 展开更多
关键词 Trace contaminants Antibiotic resistance genes(ARGs) Synergistic adsorption and photocatalytic oxidation Interfacial behaviors
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Short-term inhalation exposure evaluations of airborne antibiotic resistance genes in environments
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作者 Zhenchao Zhou Xinyi Shuai +4 位作者 Zejun Lin Lingxuan Meng Xiaoliang Ba Mark AHolmes Hong Chen 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2022年第12期62-71,共10页
Antibiotic resistance is a sword of Damocles that hangs over humans.In regards to airborne antibiotic resistance genes(AARGs),critical knowledge gaps still exist in the identification of hotspots and quantification of... Antibiotic resistance is a sword of Damocles that hangs over humans.In regards to airborne antibiotic resistance genes(AARGs),critical knowledge gaps still exist in the identification of hotspots and quantification of exposure levels in different environments.Here,we have studied the profiles of AARGs,mobile genetic elements(MGEs)and bacterial communities in various atmospheric environments by high throughput q PCR and 16S r RNA gene sequencing.We propose a new AARGs exposure dose calculation that uses short-term inhalation(STI).Swine farms and hospitals were high-risk areas where AARGs standardised abundance was more abundant than suburbs and urban areas.Additionally,resistance gene abundance in swine farm worker sputum was higher than that in healthy individuals in other environments.The correlation between AARGs with MGEs and bacteria was strong in suburbs but weak in livestock farms and hospitals.STI exposure analysis revealed that occupational intake of AARGs(via PM10)in swine farms and hospitals were 110 and 29 times higher than in suburbs,were 1.5×10^(4),5.6×10^(4)and 5.1×10^(2)copies,i.e.,61.9%,75.1%and 10.7%of the overall daily inhalation intake,respectively.Our study comprehensively compares environmental differences in AARGs to identify high-risk areas,and forwardly proposes the STI exposure dose of AARGs to guide risk assessment. 展开更多
关键词 AIRBORNE Antibiotic resistance genes(ARGs) Short-term inhalation Risk assessment
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The honeybee gut resistome and its role in antibiotic resistance dissemination
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作者 Huihui SUN Hu LI +5 位作者 Xue ZHANG Yan LIU Hao CHEN Li ZHENG Yifan ZHAI Hao ZHENG 《Integrative Zoology》 SCIE CSCD 2023年第6期1014-1026,共13页
There is now general concern about widespread antibiotic resistance,and growing evidence indicates that gut microbiota is critical in providing antibiotic resistance.Honeybee is an important pollinator;the incidence o... There is now general concern about widespread antibiotic resistance,and growing evidence indicates that gut microbiota is critical in providing antibiotic resistance.Honeybee is an important pollinator;the incidence of antibiotic resistance genes in honeybee gut causes potential risks to not only its own health but also to public and animal health,for its potential disseminator role,thus receiving more attention from the public.Recent analysis results reveal that the gut of honeybee serves as a reservoir of antibiotic resistance genes,probably due to antibiotics application history in beekeeping and horizontal gene transfer from the highly polluted environment.These antibiotic resistance genes accumulate in the honeybee gut and could be transferred to the pathogen,even having the potential to spread during pollination,tending,social interactions,etc.Newly acquired resistance traits may cause fitness reduction in bacteria whereas facilitating adaptive evolution as well.This review outlines the current knowledge about the resistome in honeybee gut and emphasizes its role in antibiotic resistance dissemination. 展开更多
关键词 antibiotic resistance genes(ARGs) EVOLUTION gut microbiota HONEYBEE TRANSMISSION
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Elevated Atmospheric CO_(2)Reduced Antibiotics Accumulation in Rice Grains and Soil ARGs Abundance in Multiple Antibiotics-contaminated Paddy Fields
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作者 WANG Yabo XU Meiling +5 位作者 WANG Xiaojie AI Fuxun DU Wenchao YIN Ying JI Rong GUO Hongyan 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2023年第3期455-464,共10页
Antibiotic resistance genes(ARGs)as new pollutants have become a global environmental pollution problem in recent years.Elevated atmospheric CO_(2) is one of the major factors affecting global climate change.But,the i... Antibiotic resistance genes(ARGs)as new pollutants have become a global environmental pollution problem in recent years.Elevated atmospheric CO_(2) is one of the major factors affecting global climate change.But,the impacts of elevated CO_(2) on soil ARGs in multiple antibiotics-contaminated paddy soils are largely unknown.In this study,six antibiotics including sulfadiazine(SDZ),sulfamethoxazole(SMZ),tetracycline(TC),oxytetracycline(OTC),enrofloxacin(ENR),and ciprofloxacin(CIP)were selected to investigate their combined effects on rice biomass,antibiotics accumulation,soil bacterial community and ARGs under elevated CO_(2) levels.Results showed that elevated CO_(2) significantly reduced the accumulation of SMZ,OTC,ENR,and CIP in rice grains by 18.98%,20.07%,41.73%,and 44.25%,respectively.Elevated CO_(2) could affect soil microbialβ-diversity,and tend to reduce the microbial functions of human diseases,organismal systems,and genetic information processing.In addition,elevated CO_(2) significantly decreased the abundance of sulfonamide ARGs,tetracycline ARGs,and quinolone ARGs by 19.59%,18.58%,and 28.96%,respectively,while increased that of multidrug ARGs by 11.54%.Overall,this study emphasized that elevated CO_(2) may mitigate the threat of antibiotics contamination to rice food security but aggravate the environmental risk of multidrug ARGs in soil,contributing to a better understanding of the consequences of elevated CO_(2) levels on food security and soil ecological health in multiple antibiotics-contaminated paddy fields. 展开更多
关键词 ANTIBIOTICS Antibiotic resistance gene(ARG) Bacterial community Elevated CO_(2) Rice
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Metagenomic insights into the profile of antibiotic resistomes in sediments of aquaculture wastewater treatment system 被引量:1
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作者 Jianfei Chen Yuyin Yang +3 位作者 Xinshu Jiang Yanchu Ke Tao He Shuguang Xie 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2022年第3期345-355,共11页
To meet the rapidly growing global demand for aquaculture products,large amounts of antibiotics were used in aquaculture,which might accelerate the evolution of antibiotic-resistant bacteria(ARB)and the propagation of... To meet the rapidly growing global demand for aquaculture products,large amounts of antibiotics were used in aquaculture,which might accelerate the evolution of antibiotic-resistant bacteria(ARB)and the propagation of antibiotic genes(ARGs).In our research,we revealed the ARGs profiles,their co-occurrence with mobile genetic elements(MGEs),and potential hosts in sediments of a crab pond wastewater purification system based on metagenomic analysis.The residual antibiotic seems to increase the propagation of ARGs in the crab pond,but there was no clear relationship between a given antibiotic type and the corresponding resistance genes.The effect of aquaculture on sediment was not as profound as that of other anthropogentic activities,but increased the relative abundance of sulfon-amide resistance gene.A higher abundance of MGEs,especially plasmid,increased the po-tential ARGs dissemination risk in crab and purification ponds.Multidrug and sulfonamide resistance genes had greater potential to transfer because they were more frequently carried by MGEs.The horizontal gene transfer was likely to occur among a variety of microorgan-isms,and various ARGs hosts including Pseudomonas,Acinetobacter,Escherichia,and Klebsiella were identified.Bacterial community influenced the composition of ARG hosts,and Pro-teobacteria was the predominant hosts.Overall,our study provides novel insights into the environmental risk of ARGs in sediments of aquaculture wastewater treatment system. 展开更多
关键词 Metagenomic sequencing AQUACULTURE Antibiotic resistome Antibiotic resistance genes(ARGs) Mobile genetic elements(MGEs) ARG hosts
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