Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous...Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus argenteus.The qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then down.The expression levels of vgr in the testis were low and only 1%-3%of that in ovary.ELISA results show that during the ovarian development of P.argenteus,VTG in liver,serum,and ovary all showed a trend from increasing to decreasing.However,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA expression.The VGR content in the testis was extremely low and stable,consistent with vgr mRNA.Immunohistochemistry results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the oocyte.Therefore,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the development of P.argenteus was revealed preliminarily.展开更多
Juvenile silver pomfret, Pampus argenteus, was grown in culture tanks for 9 weeks on four different diets, and their effects on fish growth, digestive enzyme activity, and body composition were assessed. The feeding r...Juvenile silver pomfret, Pampus argenteus, was grown in culture tanks for 9 weeks on four different diets, and their effects on fish growth, digestive enzyme activity, and body composition were assessed. The feeding regime was as follows: Diet 1: fish meat; Diet 2: fish meat+artificial feed; Diet 3: fish meat+artificial feed+Agamaki clam meat; Diet 4: fish meat+artificial feed+Agamaki clam+copepods. The greatest weight gain was associated with Diet 4, while the lowest weight gain was associated with Diet 1. No significant difference was observed in weight gain between fish receiving Diet 2 and Diet 3. Specific growth rate followed similar trends as weight gain. The feed conversion ratio (FCR) of fish fed Diet 1 was significantly higher than the other fish groups, but no significant differences were observed in FCRs of fish fed Diet 2, Diet 3 or Diet 4. There was also no significant difference in the hepatosomatic index (HSI) between the four diets. For fish that received Diets 2-4, containing artificial feed, higher protease activities were detected. A higher lipid content of the experimental diets also significantly increased lipase activities and body lipid content. No significant differences in amylase activity or body protein content were found between Diets 1-4. In conclusion, a variety of food components, including copepods and artificial feed, in the diet of silver pomfret significantly increased digestive enzyme activity and could improve growth performance.展开更多
The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±...The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±0.1 g actively preyed on both the species. Their daily A. aurita consumption was 11.6 times their own body weights regardless of the size of A. aurita medusae. Their daily R. esculent um consumption was 13, 9.1, 5, and 4.1 times their own body weights when the R. esculentum medusae were 10, 20, 30, and 40 mm in bell diameter, respectively. The survival rates of the R. esculent um were higher than those of the A. aurita. When the R. esculent um medusae were more than 30 mm in bell diameter, their survival rate exceeded 92%. Silver pomfrets serve as a type of potential predators on A. aurita in coastal waters, and they have little infl uence on R. esculent um with a size exceeding 30 mm. Besides, A. aurita may be able to be used as fi sh prey in silver pomfret artifi cial breeding.展开更多
Pampus punctatissimus (Temminck et Schlegel , 1845) and Pampus argenteus (Euphrasen , 1788) are two important species of the genus pampus and widely distributed in Chinese coastal waters . Their classification has bee...Pampus punctatissimus (Temminck et Schlegel , 1845) and Pampus argenteus (Euphrasen , 1788) are two important species of the genus pampus and widely distributed in Chinese coastal waters . Their classification has been controversial for many years . Due to the similarities of their overall appearance , many workers regarded P punctatissimus as a synonym of P argenteus Even though a few authors recognized the differences between them ,there has been great confusion on the nomenclature . Morpholoically , these two species have obvious differences in some aspects , in particular, the number of gill rakers and vertebrae, the lateral line branches in the head, the fin formula , etc.. As these differences comprised sufficient differentiation of these two species, Pampus punctatissimus (Temminck et Schlegel, 1845) was recognized as a species distinct from P argenteus (Euphrasen, 1788). A redescription of it is given in the present study.展开更多
An increasing interest of the economic operators on the bycatch of shrimp fishery endangers bycatch species to over-exploitation risks. This study provides a better knowledge regarding to the current status of one maj...An increasing interest of the economic operators on the bycatch of shrimp fishery endangers bycatch species to over-exploitation risks. This study provides a better knowledge regarding to the current status of one major component of the bycatches Otolithes argenteus （O. argenteus） caught in the shrimp fishery area along the West coast of Madagascar using the morphometric analysis. Individual body weight and body measurement were carried out on 565 specimens collected from the sale＇s society of seafood （SOPROMER） in the Capital city. Results show a high proportion of small fishes like as immature young individuals. A ＂negative allometry＂ growth type within the juveniles to become ＂isometric allometry＂ for the adult, large size was observed. Marine environment is favorable to the development for the whole population of the O. argenteus species. The morphometry approach helped to better understanding of the characteristics and the operating status of the O. argenteus population thus constitute as a tool in establishing a sustainable fisheries management strategy in Madagascar.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31872586,42076118)the Major Project of Science,Technology and Innovation 2025 in Ningbo City(No.2021Z003)the K.C.Wong Magna Fund in Ningbo University。
文摘Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus argenteus.The qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then down.The expression levels of vgr in the testis were low and only 1%-3%of that in ovary.ELISA results show that during the ovarian development of P.argenteus,VTG in liver,serum,and ovary all showed a trend from increasing to decreasing.However,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA expression.The VGR content in the testis was extremely low and stable,consistent with vgr mRNA.Immunohistochemistry results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the oocyte.Therefore,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the development of P.argenteus was revealed preliminarily.
基金Supported by the National Key Technology Research and Development Program during the Twelfth Five-Year Plan Period(No.2011BAD13B01)the Central Nonprofit Basic Scientific Research Project for the Scientific Research Institutes of China(No.East-2011M09)the Research Project Commissioned by the Shanghai Agricultural Commission(No.2007-4-1)
文摘Juvenile silver pomfret, Pampus argenteus, was grown in culture tanks for 9 weeks on four different diets, and their effects on fish growth, digestive enzyme activity, and body composition were assessed. The feeding regime was as follows: Diet 1: fish meat; Diet 2: fish meat+artificial feed; Diet 3: fish meat+artificial feed+Agamaki clam meat; Diet 4: fish meat+artificial feed+Agamaki clam+copepods. The greatest weight gain was associated with Diet 4, while the lowest weight gain was associated with Diet 1. No significant difference was observed in weight gain between fish receiving Diet 2 and Diet 3. Specific growth rate followed similar trends as weight gain. The feed conversion ratio (FCR) of fish fed Diet 1 was significantly higher than the other fish groups, but no significant differences were observed in FCRs of fish fed Diet 2, Diet 3 or Diet 4. There was also no significant difference in the hepatosomatic index (HSI) between the four diets. For fish that received Diets 2-4, containing artificial feed, higher protease activities were detected. A higher lipid content of the experimental diets also significantly increased lipase activities and body lipid content. No significant differences in amylase activity or body protein content were found between Diets 1-4. In conclusion, a variety of food components, including copepods and artificial feed, in the diet of silver pomfret significantly increased digestive enzyme activity and could improve growth performance.
基金Supported by National Basic Research Program of China(973 Program)(No.2011CB403605)the Key Science and Technology Program of Qingdao(No.11-3-1-6-hy)+1 种基金the Special Funds for the Basic R&D Programin the Central Non-Profit Research Institutes(No.20603022011008)the National Key Technology R&D Program during the Twelfth Five-Year Plan Period(No.2011BAD13B01)
文摘The current study explored Aurelia aurita and Rhopilema esculent um consumption by silver pomfret juveniles, as well as their prey selection between the two jellyfi sh species. Silver pomfret juveniles weighing 1±0.1 g actively preyed on both the species. Their daily A. aurita consumption was 11.6 times their own body weights regardless of the size of A. aurita medusae. Their daily R. esculent um consumption was 13, 9.1, 5, and 4.1 times their own body weights when the R. esculentum medusae were 10, 20, 30, and 40 mm in bell diameter, respectively. The survival rates of the R. esculent um were higher than those of the A. aurita. When the R. esculent um medusae were more than 30 mm in bell diameter, their survival rate exceeded 92%. Silver pomfrets serve as a type of potential predators on A. aurita in coastal waters, and they have little infl uence on R. esculent um with a size exceeding 30 mm. Besides, A. aurita may be able to be used as fi sh prey in silver pomfret artifi cial breeding.
文摘Pampus punctatissimus (Temminck et Schlegel , 1845) and Pampus argenteus (Euphrasen , 1788) are two important species of the genus pampus and widely distributed in Chinese coastal waters . Their classification has been controversial for many years . Due to the similarities of their overall appearance , many workers regarded P punctatissimus as a synonym of P argenteus Even though a few authors recognized the differences between them ,there has been great confusion on the nomenclature . Morpholoically , these two species have obvious differences in some aspects , in particular, the number of gill rakers and vertebrae, the lateral line branches in the head, the fin formula , etc.. As these differences comprised sufficient differentiation of these two species, Pampus punctatissimus (Temminck et Schlegel, 1845) was recognized as a species distinct from P argenteus (Euphrasen, 1788). A redescription of it is given in the present study.
文摘An increasing interest of the economic operators on the bycatch of shrimp fishery endangers bycatch species to over-exploitation risks. This study provides a better knowledge regarding to the current status of one major component of the bycatches Otolithes argenteus （O. argenteus） caught in the shrimp fishery area along the West coast of Madagascar using the morphometric analysis. Individual body weight and body measurement were carried out on 565 specimens collected from the sale＇s society of seafood （SOPROMER） in the Capital city. Results show a high proportion of small fishes like as immature young individuals. A ＂negative allometry＂ growth type within the juveniles to become ＂isometric allometry＂ for the adult, large size was observed. Marine environment is favorable to the development for the whole population of the O. argenteus species. The morphometry approach helped to better understanding of the characteristics and the operating status of the O. argenteus population thus constitute as a tool in establishing a sustainable fisheries management strategy in Madagascar.
