期刊文献+
共找到69篇文章
< 1 2 4 >
每页显示 20 50 100
Interaction between seed size and NaCl on germination and early seedling growth of some Turkish cultivars of chickpea(Cicer arietinum L.) 被引量:9
1
作者 Muharrem KAYA Gamze KAYA +4 位作者 Mehmet Demir KAYA Mehmet ATAK Sevil SAGLAM Khalid Mahmood KHAWAR Cemalettin Yasar CIFTCI 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第5期371-377,共7页
Chickpea is an important food legume crop of Turkey and is largely grown for human consumption on low moisture or salt-affected soils. The objective of the study was to find the effects of NaCl stress at electrical co... Chickpea is an important food legume crop of Turkey and is largely grown for human consumption on low moisture or salt-affected soils. The objective of the study was to find the effects of NaCl stress at electrical conductivities of 4.5, 8.6, 12.7 and 16.3 dS/m and seed sizes (7, 8 and 9 mm) on germination and early seedling growth of three popular chickpea cultivars (AKN-97, Gokce and Uzunlu-99). Mean frequency of germination, germination time, germination index, root length, shoot length and seedling fresh weight showed seed size-dependent responses ofcultivars to salt stress. In general, small seeds germinated and grew more rapidly compared to medium and large seeds of the same cultivars against all levels of salt stress, with the best results in cultivar Uzunlu-99. No effect of NaCl treatments was observed on frequency of germination; however, a drastic decrease in early seedling growth was recorded at increased NaC1 concentrations. Regression analysis results showed a significantly positive relationship (P〈0.01) between seed size and mean germination time, whereas a significantly negative relationship was recorded between seed size and germination index, root length, shoot length. Moreover, linear regression values apparently confirmed that increased seed size in each cultivar affected decreased germination index, root and shoot lengths with enhanced mean germination time. Thus, it was concluded that the use of small seeds could considerably reduce the production costs of chickpea in salt-affected soils. 展开更多
关键词 Chickpea (Cicer arietinum L.) NaCl stress CULTIVAR Seed size GERMINATION
下载PDF
Effect of Cultivar, Irrigation and Nitrogen Fertilization on Chickpea (<i>Cicer arietinum</i>L.) Productivity 被引量:1
2
作者 Kico Dhima Ioannis Vasilakoglou +1 位作者 Stefanos Stefanou Ilias Eleftherohorinos 《Agricultural Sciences》 2015年第10期1187-1194,共8页
A 2-year field study was conducted in northern Greece to investigate the effect of nitrogen fertilization and irrigation on productivity of three Greek chickpea varieties (“Amorgos” “Serifos”, “Andros”). Chickpe... A 2-year field study was conducted in northern Greece to investigate the effect of nitrogen fertilization and irrigation on productivity of three Greek chickpea varieties (“Amorgos” “Serifos”, “Andros”). Chickpea, grown under irrigation regime (30 + 30 mm of water) and fertilized with 50 kg·N·ha-1 before planting and with 40 kg·N·ha-1 at blossom growth stage, produced more total dry biomass and seed yield as compared with that grown under non-irrigated conditions and fertilized with 50 kg·N·ha-1 before planting only. In particular, irrigation and nitrogen fertilization at blossom growth stage increased total dry weight of chickpea by 18.3% and 18.5%, respectively, as compared with that of non-irrigated and fertilized with N before planting. The corresponding increase of seed yield was 30.5% and 20%, respectively. The total dry biomass of “Amorgos” was 10% and 13% greater than that of “Serifos” and “Andros”, while its respective seed yield increase was 5% and 16%. Finally, the quantum yield of photosystem II of chickpea was not affected by irrigation or fertilization. These results indicated that nitrogen fertilization at blossom growth stage combined with irrigation increased seed yield of all chickpea varieties, whereas the same treatments did not have any effect on plant quantum yield of photosystem II. 展开更多
关键词 CHICKPEA (Cicer arietinum L.) IRRIGATION Nitrogen Fertilization Dry Biomass Seed YIELD Quantum YIELD of Photosystem II
下载PDF
Yield and Water Productivity of Chickpea (<i>Cicer arietinum</i>L.) as Influenced by Different Irrigation Regimes and Varieties under Semi Desert Climatic Conditions of Sudan 被引量:1
3
作者 M. K. Alla Jabow O. H. Ibrahim H. S. Adam 《Agricultural Sciences》 2015年第11期1299-1308,共10页
A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on ch... A field experiment was conducted at Hudeiba Research Station Farm, located at Ed-Damer, Sudan during 2011/2012 and 2012/2013 winter seasons to investigate the effect of different irrigation regimes and varieties on chickpea (Cicer arietinum L.) yield, yield components and water productivity. The treatments include three irrigation regimes;irrigation every 10 days (I1 = full irrigation), irrigation every 15 days (I2 = moderate stress) and irrigation every 20 days (I3 = severe stress) and two varieties (Borgieg and Wad Hamid). The treatments were arranged in factorial randomized complete block design (RCBD) with 3 replications. Irrigation water being applied, grain yield, yield components (number of pods per plant, number of seeds per pod and the 100 seeds weight) and crop water productivity (CWP) and irrigation water productivity (IWP) were recorded. Results showed that the number of pods per plant, number of seeds per pod, 100-seeds weight, grain yield and irrigation water applied were significantly (p ≤ 0.001) affected by irrigation regimes. The highest values of these traits obtained with full irrigation, whereas the lowest values were recorded under severe water stress conditions. Results also indicated that, moderate and severe water stress regimes saved irrigation water by 24% and 32%, respectively compared with full irrigation. This study indicated that treatment I1 which was irrigated every 10-days did not produce the highest IWP, while treatment I2 which irrigated every 15-days gave the highest IWP. The lowest IWP occurred at severe water stress regime (I3). It could be concluded that moderate water stress might be adopted. Contrarily, the adoption of severe water stressed that produce high water savings would lead to yield losses that might be economically not acceptable. The late maturing chickpea variety of Borgieg significantly (p ≤ 0.05) out-yielded the early maturing variety Wad Hamid by 11%. Borgieg displayed the highest values of CWP and IWP. 展开更多
关键词 WATER Stress CICER arietinum L. Borgieg WAD Hamid WATER Productivity
下载PDF
Rhizogenesis of Two Species Fabaceae: <i>Cicer arietinum</i>L. and <i>Pisum sativum</i>L.
4
作者 Beddi Mohammed Benabadji Noury 《Open Journal of Ecology》 2018年第4期239-249,共11页
Our work of comparative study on the biomorphological, rhizogenic level of Chickpea (Cicer arietinum) and Pea (Pisum sativum), includes successively the following results: The in-vitro culture done, showed strains, wh... Our work of comparative study on the biomorphological, rhizogenic level of Chickpea (Cicer arietinum) and Pea (Pisum sativum), includes successively the following results: The in-vitro culture done, showed strains, which can reach 6.5 mm in the medium Nutrient agar (NA) for Cicer arietinum 6 mm of strain were recorded in the PDA culture medium for Pisum sativum. The best strain is obtained in temperature of 20&#8451;(ambient). According to the results of the rhizogenesis, we notice that the nutrient agar (NA) medium responds better that the PDA medium for Cicer arietinum. Meanwhile, the medium PDA brings a better reaction (response) compared with the nutrient agar medium concerning Pisum sativum. 展开更多
关键词 PISUM sativum L. (pea) CICER arietinum L. (chickpea) RHIZOGENESIS
下载PDF
Variation in Thermal time model Parameters Between Two Contrasting Chickpea (<i>Cicer arietinum</i>) cultivars
5
作者 Atif Hassan Naim Faisal El Gasim Ahmed 《Agricultural Sciences》 2015年第12期1421-1427,共7页
A laboratory experiment was carried out to determine the effect of different constant temperatures on germination and early seedling establishment and to study the variation among parameters of thermal time model para... A laboratory experiment was carried out to determine the effect of different constant temperatures on germination and early seedling establishment and to study the variation among parameters of thermal time model parameters for two contrasting chickpea cultivars . Seeds were subjected to six constant temperatures from 10 o C to 35 o C . A complete randomized design was used with four replication. Analysis of variance showed significant differences among treatments for all characters studied. The final germination percentage significantly increased with increasing temperature up to 25 ° C, and thereafter there was a sharp decrease in final germination at 30 ° and 35 ° C. Desi type cultivar (small seeded) “Jabel Marra” significantly exhibited higher final germination percentage and lower germination rate compared with the kabui type cultivar “Shendi” at all temperatures. The median (θ T(50) ) of the thermal time was significantly differ between the two chickpea cultivars. The large seeded cultivars (shendi) recorded significantly higher median thermal time than the small seeded cultivars (Jabel Marra). The results also revealed a significant differences between the two cultivars in all thermal time model parameters. The small seeded cultivar (Jabel Marra) scored lower total dry matter and temperature tolerance index (TTI) compared to the large seeded cultivar (Shendi) at all temperatures studied. 展开更多
关键词 CICER arietinum CHICKPEA Base temperture GERMINATION Thermal time model
下载PDF
A Kunitz trypsin inhibitor from chickpea (<i>Cicer arietinum</i>L.) that exerts an antimicrobial effect on Fusarium oxysporum f.sp. ciceris
6
作者 Meera Nair Sardul Singh Sandhu 《Agricultural Sciences》 2013年第11期585-594,共10页
Fusarium oxysporum f.sp. ciceris (Foc) is one of the most important fungal pathogens of chickpea and is regarded as a constant threat in tropical and subtropical countries. In order to correlate Fusarium wilt resistan... Fusarium oxysporum f.sp. ciceris (Foc) is one of the most important fungal pathogens of chickpea and is regarded as a constant threat in tropical and subtropical countries. In order to correlate Fusarium wilt resistance/susceptibility in Cicer arietinum to the presence or absence of trypsin inhibitor (TI) in the crude extract, trypsin inhibitory assay (TIA) and in vitro activity of TI against Foc were studied. In the present study, a 20 kDa trypsin inhibitor was purified from Fusarium wilt resistant cultivar (viz. JG 2001-12) by ammonium sulfate precipitation, dialysis and chromatographies with Sephadex G-100 and Diethyl aminoethyl cellulose (DEAE-cellulose-52) ion-exchange column. Results of pathogenecity assay were found to be in correlation to the trypsin inhibitor assay where the Fusarium wilt resistant cultivar showed high trypsin inhibitory activity (99%) in the presence of trypsin enzyme using both natural and synthetic substrates. Preliminary studies using crude extracts of JG 2001-12 showed a decrease in radial growth of Foc. A 45%-82% reduction in conidium germination at 20 μg&middotmL-1?Cicer arietinum trypsin inhibitor (CaTI) concentration was observed, thereby, indicating the use of CaTI in suppression of pathogen and in its deployment through transgenic plants for the management of Fusarium wilt. 展开更多
关键词 CICER arietinum Fusarium Wilt KUNITZ PROTEINASE INHIBITOR Trypsin INHIBITOR
下载PDF
Effect of Cooking on Amylose Contents of Mungbean (Phaseolus aureus) and Chickpea (Cicer arietinum L.) Varieties
7
作者 N. Bibil Z. Mehmood +2 位作者 A. Zeb M. Khan R. A. Anis 《Journal of Food Science and Engineering》 2011年第1期56-61,共6页
Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean ... Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean (NM-92, NM-98 and Ramazan) and kabuli type chickpea (CMNKI0-99, CMNK452-2 and Hassan 2k) varieties and quantifying the influence of cooking (boiling) on the amylose contents of pulses. The data revealed that moisture and arnylose content of uncooked mungbean varieties ranged 9.01-9.47% and 20.06-22.26% respectively. The moisture and amylose content of Kabuli type chickpea varieties varied 8.52-8.79% and 20.25-22.83% respectively. Moisture content of mungbean (64.16-66.08%) as well as chickpea (55.56-61.52%) varieties increased after cooking. For all the three varieties of mungbean the observed cooking time was 11 minutes. The maximum value of amylose content was assayed for NM-98 (20.74%) followed by Ramzan (20.24%). The cooking time of Kabuli type chickpea varieties CMNK-452-2 and CMNK-10-99 was 60 min. Hassan 2K observed more cooking time (90 min) and highest value of amylose content in cooked (19.01%) samples. The effect of cooking/varietals on sensory evaluation (appearance, flavor, texture and overall acceptability) of mungbean varieties was statistically non significant attributes. Among chickpea varieties, Hassan 2K was ranked lowest due to its small size and hard texture and the sensory attributes were statistically significant (P 〈 0.05) as compared to the other two varieties. 展开更多
关键词 Amylose and cooking Mungbean (Phaseolus aureus) Chickpea (Cicer arietinum L.)
下载PDF
人工老化对鹰嘴豆种子发芽和膜透性的影响 被引量:1
8
作者 吴旭鹏 张小燕 《青海畜牧兽医杂志》 2023年第2期19-23,共5页
以鹰嘴豆种子为研究材料,分别进行12 h、24 h、36 h、48 h、60 h、72 h、84 h及96 h 8个人工老化处理(40℃、相对湿度100%),研究老化过程中种子的发芽率、发芽指数、活力指数、苗长、根长、电导率及可溶性糖的变化趋势,分析人工老化处... 以鹰嘴豆种子为研究材料,分别进行12 h、24 h、36 h、48 h、60 h、72 h、84 h及96 h 8个人工老化处理(40℃、相对湿度100%),研究老化过程中种子的发芽率、发芽指数、活力指数、苗长、根长、电导率及可溶性糖的变化趋势,分析人工老化处理对鹰嘴豆种子膜透性的影响。结果表明,鹰嘴豆种子在老化处理后,发芽率、发芽指数先急剧下降(12 h)而后缓慢下降,浸种液电导率先缓慢上升而后急剧上升(48 h),而可溶性糖含量先急剧上升(12 h)后缓慢上升再略有下降(96 h)。不同老化程度种子的电导率与发芽指数和发芽率均呈显著正相关(P<0.05),发芽率与发芽指数呈显著正相关(P<0.05),可溶性糖与活力指数呈极显著正相关(P<0.01),其他各指标间无显著相关性。 展开更多
关键词 鹰嘴豆 种子 老化 发芽 膜透性
下载PDF
鹰嘴豆种质资源农艺性状遗传多样性分析 被引量:92
9
作者 聂石辉 彭琳 +1 位作者 王仙 季良 《植物遗传资源学报》 CAS CSCD 北大核心 2015年第1期64-70,共7页
以100份鹰嘴豆种质资源为材料,应用聚类分析和主成分分析方法,对15个主要农艺性状的遗传多样性进行分析。结果表明,参试材料存在广泛的遗传多样性。其中,多样性指数最高的是株高,其次是百粒重;性状变异系数最大的是单株荚数,其次是单株... 以100份鹰嘴豆种质资源为材料,应用聚类分析和主成分分析方法,对15个主要农艺性状的遗传多样性进行分析。结果表明,参试材料存在广泛的遗传多样性。其中,多样性指数最高的是株高,其次是百粒重;性状变异系数最大的是单株荚数,其次是单株粒重;基于各种质间形态标记的遗传差异,将100份鹰嘴豆种质聚类并划分为4大类群。第Ⅰ类群可作为选育丰产中粒型和株高适中的品种,第Ⅱ类群可作为选育矮秆耐密及特异粒色(型)品种,第Ⅲ类群丰产性较差可作为选育子粒球型、光滑的品种,第Ⅳ类群可作为选育大粒型、适宜机械化收获的品种。9个数量性状的主成分分析结果表明,前4个主成分累计贡献率达73.91%,各主成分性状载荷值反映了主要数量性状的育种选择潜力。综合分析种质资源农艺性状,为鹰嘴豆的有效利用提供一定的科学依据。 展开更多
关键词 鹰嘴豆 种质资源 遗传多样性 农艺性状
下载PDF
鹰咀豆根系分泌物的分离鉴定及典型分泌物苯甲醛的化感效应 被引量:19
10
作者 柴强 黄高宝 +2 位作者 黄鹏 张恩和 冯福学 《草业学报》 CSCD 2005年第1期106-111,共6页
运用CH2Cl2提取水培35d鹰咀豆的根系分泌物,并通过气相色谱和质谱联用技术(GC MS)进行分离鉴定,对典型分泌物苯甲醛的生物学效应通过发芽试验进行了测试。结果表明,鹰咀豆的根分泌物主要包括有机酸、酚、烃类、酯、酮类、醇、酰胺、苯... 运用CH2Cl2提取水培35d鹰咀豆的根系分泌物,并通过气相色谱和质谱联用技术(GC MS)进行分离鉴定,对典型分泌物苯甲醛的生物学效应通过发芽试验进行了测试。结果表明,鹰咀豆的根分泌物主要包括有机酸、酚、烃类、酯、酮类、醇、酰胺、苯、醛和噻唑等;苯甲醛是一种他感作用强于自毒作用的化感物质,对2种幼苗组成的复合系统的负效应小于对单作系统的负效应。 展开更多
关键词 根系分泌物 化感作用 鹰咀豆 苯甲醛
下载PDF
富钒鹰嘴豆芽对糖尿病大鼠血糖和血脂的影响 被引量:11
11
作者 毛雪琴 张玲 +3 位作者 王梦欣 孙兆峰 夏作理 杨晓达 《中华中医药杂志》 CAS CSCD 北大核心 2008年第6期498-501,共4页
目的:研究富钒鹰嘴豆芽降糖作用及可能机制。方法:以链脲佐菌素诱导形成糖尿病大鼠模型,饲喂不同浓度的富钒鹰嘴豆芽4周,观察大鼠血糖和血脂等的变化。结果:与正常对照组比较,糖尿病模型大鼠的血糖升高了4倍,血浆胰岛素降低了57.63%、... 目的:研究富钒鹰嘴豆芽降糖作用及可能机制。方法:以链脲佐菌素诱导形成糖尿病大鼠模型,饲喂不同浓度的富钒鹰嘴豆芽4周,观察大鼠血糖和血脂等的变化。结果:与正常对照组比较,糖尿病模型大鼠的血糖升高了4倍,血浆胰岛素降低了57.63%、糖化血红蛋白(GHb)升高了42.28%,谷丙转氨酶、谷草转氨酶和血浆脂质水平明显升高。而分别饲喂不同浓度富钒鹰嘴豆芽4周后,糖尿病大鼠的上述指标都得到改善,其中以中等剂量(100μg/ml)富钒鹰嘴豆芽治疗组的效果最明显:血糖水平降低了58.60%(P<0.05);血浆胰岛素水平明显增高(P<0.05)且接近正常;GHb降低了32.81%,血浆低密度脂蛋白、甘油三酯和总胆固醇明显下降(P<0.05),谷丙转氨酶和谷草转氨酶分别降低了39.98%和41.37%。而葡萄糖耐量得到显著改善,2h的血糖值降低了38.46%。结论:富钒鹰嘴豆芽能阻止糖尿病大鼠高血糖的进展和血浆脂质水平的改变;这种钒的有机复合物形式达到了减毒增效的目的,具有较佳的应用前景。 展开更多
关键词 原钒酸钠 鹰嘴豆芽 链脲佐菌素糖尿病大鼠 萌芽 有机钒化合物 实验研究
下载PDF
鹰嘴豆种子胰蛋白酶抑制剂的分离纯化与鉴定 被引量:8
12
作者 赵欣 付煊赫 +2 位作者 张宗申 刘同祥 王继峰 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2011年第3期287-292,共6页
为了寻找具有药物作用的天然胰蛋白酶抑制物,采用硫酸铵分级沉淀、离子交换层析(DEAE-纤维素52)及Sephadex G-100凝胶层析等方法,从鹰嘴豆种子中分离出一种鹰嘴豆胰蛋白酶抑制剂(CPTI).研究表明:CPTI对胰蛋白酶有较强的抑制作用,抑制率... 为了寻找具有药物作用的天然胰蛋白酶抑制物,采用硫酸铵分级沉淀、离子交换层析(DEAE-纤维素52)及Sephadex G-100凝胶层析等方法,从鹰嘴豆种子中分离出一种鹰嘴豆胰蛋白酶抑制剂(CPTI).研究表明:CPTI对胰蛋白酶有较强的抑制作用,抑制率达80%,而对胰凝乳蛋白酶抑制作用较弱,抑制率为32%,对胃蛋白酶、木瓜蛋白酶及枯草杆菌蛋白酶均无抑制作用;用SDS-PAGE测得CPTI近似分子质量为25.7 kD;CPTI具有较高的热稳定性,在100℃下加热60min,对胰蛋白酶活性仍保持78%抑制率;Lineveaer-Burk作图得知该抑制剂属竞争性抑制类型.动力学测定显示,来自鹰嘴豆中的CPTI对胰蛋白酶的抑制作用常数(Ki)为3.99×10-7 mol/L. 展开更多
关键词 鹰嘴豆 胰蛋白酶抑制剂 分离纯化 鉴定
下载PDF
GC-MS应用于鹰嘴豆中弱极性化学成分的研究(英文) 被引量:5
13
作者 吴霞 张秀 +1 位作者 叶蕴华 周亚伟 《北京大学学报(自然科学版)》 EI CAS CSCD 北大核心 2007年第2期166-168,共3页
应用气相色谱-质谱分析技术,首次对鹰嘴豆中弱极性成分进行研究。从鹰嘴豆弱极性萃取部位中鉴定了22个化合物,以脂肪酸为主要成分。脂肪酸含量(质量分数)高达81.25%,其中9-十八烯酸和8,11-十八碳二烯酸的含量分别占总量的28.00%和38.60%。
关键词 气相色谱-质谱 弱极性部位 脂肪酸 鹰嘴豆 化学成分
下载PDF
鹰嘴豆化学成分研究 被引量:24
14
作者 谭永霞 孙玉华 陈若芸 《中国中药杂志》 CAS CSCD 北大核心 2007年第16期1650-1652,共3页
目的:研究鹰嘴豆的化学成分,从中寻找具有生物活性的天然化合物。方法:采用不同体积分数的乙醇对鹰嘴豆干燥种子及鹰嘴豆发芽种子进行提取,经硅胶、大孔树脂D101、葡聚糖Sephadex LH-20色谱手段分离纯化,通过NMR,MS等波谱分析手段鉴定... 目的:研究鹰嘴豆的化学成分,从中寻找具有生物活性的天然化合物。方法:采用不同体积分数的乙醇对鹰嘴豆干燥种子及鹰嘴豆发芽种子进行提取,经硅胶、大孔树脂D101、葡聚糖Sephadex LH-20色谱手段分离纯化,通过NMR,MS等波谱分析手段鉴定化合物结构。结果:分离到9个化合物,分别鉴定为3-羟基-齐墩果-12-烯(1),鹰嘴豆芽素A-7-O-β-D-葡萄糖苷(2),脑苷(3),1-乙基-α-L-半乳糖苷(4),尿苷(5),腺苷(6),色氨酸(7),鹰嘴豆芽素A(8),芒柄花素(9)。结论:1,3,4,6,7均为首次从鹰嘴豆属植物中分离到。 展开更多
关键词 豆科 鹰嘴豆 化学成分
下载PDF
鹰嘴豆总皂苷对2型糖尿病大鼠抗氧化作用的影响 被引量:4
15
作者 凯赛尔·阿不都克热木 霍仕霞 +3 位作者 魏媛媛 闫冬 阿吉艾克拜尔·艾萨 帕尔哈提·克热木 《新疆医科大学学报》 CAS 2011年第7期705-709,共5页
目的观察鹰嘴豆总皂苷(TSCA)对2型糖尿病大鼠抗氧化作用的影响。方法以高脂饲料喂养后的雄性Wistar大鼠腹腔注射链脲佐菌素(STZ)诱导2型糖尿病模型,其中两组造模动物分别灌胃给予鹰嘴豆总皂苷100 mg/kg(低剂量组),300 mg/kg(高剂量组)。... 目的观察鹰嘴豆总皂苷(TSCA)对2型糖尿病大鼠抗氧化作用的影响。方法以高脂饲料喂养后的雄性Wistar大鼠腹腔注射链脲佐菌素(STZ)诱导2型糖尿病模型,其中两组造模动物分别灌胃给予鹰嘴豆总皂苷100 mg/kg(低剂量组),300 mg/kg(高剂量组)。4周后,测定各组大鼠肝脏、肾脏、肺、骨骼肌等组织中相关抗氧化指标。结果与模型组比较,鹰嘴豆总皂苷低剂量组能够显著升高肾脏中总超氧化物岐化酶(TSOD)水平(P<0.01)、肝脏、肾脏、骨骼肌中总一氧化氮合酶(TNOS)活力(P<0.01)和肺、骨骼肌中谷胱甘肽过氧化物酶(GSH-PX)活性(P<0.01),且能升高骨骼肌中总超氧化物岐化酶(TSOD)水平及过氧化氢酶(CAT)活力(P<0.05),显著降低肝脏、骨骼肌中丙二醛(MDA)的含量(P<0.01);鹰嘴豆总皂苷高剂量组能够显著升高肾脏总超氧化物岐化酶(TSOD)水平(P<0.01)和肝脏、肾脏、骨骼肌中TNOS活力(P<0.01),且能升高骨骼肌总超氧化物岐化酶(TSOD)水平、谷胱甘肽过氧化物酶(GSH-PX)活性、过氧化氢酶(CAT)活力、肾脏过氧化氢酶(CAT)活力(P<0.05),显著降低肝脏MDA的含量(P<0.01)。结论此研究提示鹰嘴豆总皂苷可以改善2型糖尿病大鼠的抗氧化能力。 展开更多
关键词 鹰嘴豆总皂苷 糖尿病 2型糖尿病 抗氧化作用
下载PDF
高活性大豆异黄酮糖苷酶的分离原料筛选、纯化及性质研究 被引量:2
16
作者 潘利华 罗建平 +2 位作者 罗水忠 徐学玲 王贵娟 《食品科学》 EI CAS CSCD 北大核心 2009年第1期164-168,共5页
通过对32种植物β-葡萄糖苷酶水解染料木苷的活性比较,发现鹰嘴豆β-葡萄糖苷酶具有4.19U/mg的大豆异黄酮糖苷水解酶活性。该酶经硫酸铵分级沉淀、DEAE-Cellulose-52离子交换、Sephadex G-100凝胶层析纯化,纯化了10.1倍,收率为7.2%;SDS-... 通过对32种植物β-葡萄糖苷酶水解染料木苷的活性比较,发现鹰嘴豆β-葡萄糖苷酶具有4.19U/mg的大豆异黄酮糖苷水解酶活性。该酶经硫酸铵分级沉淀、DEAE-Cellulose-52离子交换、Sephadex G-100凝胶层析纯化,纯化了10.1倍,收率为7.2%;SDS-PAGE和Sephadex G-100凝胶层析结果表明,该酶的分子量为73.2kD,含两个亚基;该酶的最适反应温度为45℃;最适pH6.0;当以染料木苷和大豆苷为底物时该酶的米氏常数分别为11.0和19.0μg/ml。温度在30~45℃、pH值在4.5~6.5范围内该酶较稳定:Ag^+、Hg^(2+)和D-葡萄糖酸内酯对该酶有强烈抑制作用。该酶能水解染料木苷和大豆苷,但不能水解纤维二糖和α-乳糖。 展开更多
关键词 大豆异黄酮糖苷水解酶 Β-葡萄糖苷酶 染料木苷 鹰嘴豆 酶学性质
下载PDF
鹰嘴豆β-半乳糖苷酶的分离纯化与表征 被引量:2
17
作者 李绪渊 赵克浩 +1 位作者 孟延发 涂伟霞 《物理化学学报》 SCIE CAS CSCD 北大核心 1996年第7期629-634,共6页
从鹰嘴豆中分离得到了三种β-半乳糖苷酶(酶Ⅰ、酶Ⅱ和酶Ⅲ)。将酶Ⅰ和酶Ⅱ进一步纯化,其比活力分别提高了19倍和48倍.酶活力回收率分别为16%和18%,测得它们的表观分子里分别为2.4×104和5.8×104;最适pH分别5.9和5.... 从鹰嘴豆中分离得到了三种β-半乳糖苷酶(酶Ⅰ、酶Ⅱ和酶Ⅲ)。将酶Ⅰ和酶Ⅱ进一步纯化,其比活力分别提高了19倍和48倍.酶活力回收率分别为16%和18%,测得它们的表观分子里分别为2.4×104和5.8×104;最适pH分别5.9和5.0,最适温度分别为55℃和45℃.酶Ⅰ水解ONPG和PNPG的KM分别为33×10-2mol·dm-3和60×10-3mol·dm-3;酶Ⅱ水解ONPG和PNPG的KM分别为30×10-3mol·dm-3和60×10-4mol·dm-3.乳糖和半乳糖为该酶的竞争性可逆抑制剂,棉子糖为非竞争性可逆抑制剂.该酶受Hg2+和PCMB强烈抑制和NEM明显抑制,而Mg2+、Zn2+和Ca2+具有激活作用,推知巯基(-SH)是酶活性中心必须基团. 展开更多
关键词 Β-半乳糖苷酶 鹰嘴豆 分离纯化 表征 酶催化作用
下载PDF
鹰嘴豆胰蛋白酶抑制剂的提取工艺研究 被引量:3
18
作者 付煊赫 刘同祥 +1 位作者 张宗申 王继峰 《时珍国医国药》 CAS CSCD 北大核心 2010年第2期404-405,共2页
目的研究鹰嘴豆中胰蛋白酶抑制剂的最佳提取条件。方法以提取液对胰蛋白酶的抑制率为指标,采用正交实验法对提取温度(A)、溶剂用量(B)、pH值(C)、提取时间(D)4个因素进行优选,并对优选的提取方案进行验证。结果在所选水平范围内,因素C和... 目的研究鹰嘴豆中胰蛋白酶抑制剂的最佳提取条件。方法以提取液对胰蛋白酶的抑制率为指标,采用正交实验法对提取温度(A)、溶剂用量(B)、pH值(C)、提取时间(D)4个因素进行优选,并对优选的提取方案进行验证。结果在所选水平范围内,因素C和B对提取效果有显著的影响,因素D和A影响不显著。结论该文属国内首次关于鹰嘴豆胰蛋白酶抑制剂成分的研究报告,其有效部位的最佳提取工艺为C1B3D1A3。 展开更多
关键词 鹰嘴豆 胰蛋白酶抑制剂 正交实验
下载PDF
鹰嘴豆β-半乳糖苷酶的提取及其氨基酸组成研究 被引量:7
19
作者 李绪渊 朱宗祯 +2 位作者 涂伟霞 马建泰 孟延发 《甘肃科学学报》 1997年第2期12-16,共5页
从鹰嘴豆种子中分离到3种β-半乳糖苷酶(酶Ⅰ、酶Ⅱ和酶Ⅲ)。将酶Ⅰ和酶Ⅱ纯化后,其比活力分别提高了19倍和48倍,测得它们的表观分子量分别为24000和58000。酶Ⅰ含18种氨基酸,171个氨基酸残基;酶Ⅱ含16种... 从鹰嘴豆种子中分离到3种β-半乳糖苷酶(酶Ⅰ、酶Ⅱ和酶Ⅲ)。将酶Ⅰ和酶Ⅱ纯化后,其比活力分别提高了19倍和48倍,测得它们的表观分子量分别为24000和58000。酶Ⅰ含18种氨基酸,171个氨基酸残基;酶Ⅱ含16种氨基酸,305个氨基酸残基; 展开更多
关键词 鹰嘴豆 Β-半乳糖苷酶 分离提纯 氨基酸组成
下载PDF
鹰嘴豆生物学特性及应用价值 被引量:14
20
作者 朱锦福 刁治民 李强峰 《青海草业》 2004年第4期27-31,共5页
鹰嘴豆是一种具有较高价值的经济植物。本文论述鹰嘴豆的生物学特性、营养成分、经济价值及开发应用前景。
关键词 鹰嘴豆(Cicer arietinum L.) 生物学特性 应用价值
下载PDF
上一页 1 2 4 下一页 到第
使用帮助 返回顶部