敲降lncRNA UCA1降低人膀胱癌细胞系T24对吉西他滨耐药

目的探讨lncRNA UCA1对人膀胱癌细胞系T24体外对吉西他滨(GEM)耐药的影响及相关分子机制。方法用RT-qPCR检测T24细胞和T24/GEM细胞中UCA1 mRNA表达。用不同浓度GEM(0.1、1和10μmol/L)刺激T24/GEM细胞48 h,用LC3染色法观察自噬斑,Western blot检测自噬相关蛋白质表达。将UCA1-shRNA或UCA1-shRNA+pcDNA-Bcl-2转入T24/GEM细胞,用MTT法和流式细胞测量术评估细胞对GEM的敏感性;用Western blot检测p53、Bcl-2和自噬相关蛋白质的表达。结果T24/GEM细胞中UCA1的表达显著高于亲本T24细胞(P<0.05)。在0~10μmol/L浓度范围的GEM呈依赖性促进T24/GEM细胞自噬(P<0.05)。敲降UCA1后,T24/GEM细胞对GEM的敏感性增加(P<0.05),自噬能力减弱(P<0.05),p53和Bcl-2表达降低(P<0.05)。Bcl-2过表达能部分逆转UCA1-shRNA对T24/GEM细胞的GEM增敏和抑制自噬的作用(P<0.05)。结论敲降lncRNA UCA1通过抑制Bcl-2介导的自噬降低T24/GEM细胞对GEM的耐药性。

长链非编码RNA TUG1和UCA1在结肠癌组织中的表达及临床意义

目的探究长链非编码RNA TUG1和UCA1在结肠癌组织中的表达及临床意义。方法选取2018年5月—2020年5月收治的80例结肠癌患者为研究对象,利用原位杂交与qRT-PCR法检测TUG1和UCA1的表达,对表达情况和患者病理特征及预后进行研究。结果UCA1和TUG1在结直肠癌组织表达过程中与性别、年龄以及病理情况关系较小(P>0.05),和患者肿瘤大小、是否存在淋巴结转移、疾病的分化情况以及分期情况高度相关(P<0.05)。UCA1和TUG1高表达患者肿瘤更大,淋巴结存在转移情况,分化程度较高且TNM分期以Ⅰ/Ⅱ为主;80例患者进行随访36个月,中位生存时间33.75个月,死亡人数16例,术后总生存率80.00%(64/80);Kaplan-Meier生存分析显示UCA1和TUG1高表达死亡12例,生存38例,生存率76.00%(38/50);UCA1和TUG1低表达死亡4例,生存26例,生存率86.66%(26/30)。结论直肠癌组织中lncRNA TUG1和UCA1高表达与低表达对患者疾病预后有一定影响。

lncRNA UCA1在急性髓系白血病患者中的表达及其临床意义

目的:检测急性髓系白血病(AML)患者骨髓中尿路上皮癌胚抗原1(lncRNA UCA1)的表达水平,并探讨lnc RNA UCA1表达水平在AML患者中的临床意义。方法:收集50例AML患者骨髓标本作为实验组和20例缺铁性贫血(IDA)患者骨髓标本作为对照组,并收集AML患者的相关临床病理特征资料。采用实时荧光定量PCR(q RT-PCR)检测lnc RNA UCA1在实验组和对照组中的表达水平,并分析lnc RNA UCA1在AML患者中的表达水平与临床病理特征及预后的关系。采用Kaplan-Meier曲线分析lnc RNA UCA1表达水平对AML患者总体生存时间(OS)的影响;采用Cox风险回归模型分析影响AML患者预后的因素。结果:与对照组相比,lnc RNA UCA1表达水平在AML患者中明显升高(P<0.001);lnc RNA UCA1高表达组中血红蛋白低于90 g/L的患者占比明显高于低表达组(P=0.004);lnc RNA UCA1在M1、M2和M4型AML患者中表达水平较高,而在M0、M5型AML患者中表达水平较低,差异具有统计学意义(P=0.009)。lnc RNA UCA1高表达组患者与低表达组患者的性别、年龄、WBC计数、PLT计数、骨髓原始细胞比例、化疗方案及疗效、核型、基因突变及预后风险分层的差异均无统计学意义(均P>0.05);lnc RNA UCA1高表达组患者的OS较低表达组患者显著缩短(P=0.0229)。结论:lnc RNA UCA1表达水平在AML患者中明显上调,其高表达与较差的临床病理特征及不良预后相关,可作为判断AML患者预后的指标和潜在的治疗靶点。

Deep brain implantable microelectrode arrays for detection and functional localization of the subthalamic nucleus in rats with Parkinson’s disease

The subthalamic nucleus(STN)is considered the best target for deep brain stimulation treatments of Parkinson’s disease(PD).It is difficult to localize the STN due to its small size and deep location.Multichannel microelectrode arrays(MEAs)can rapidly and precisely locate the STN,which is important for precise stimulation.In this paper,16-channel MEAs modified with multiwalled carbon nanotube/poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate)(MWCNT/PEDOT:PSS)nanocomposites were designed and fabricated,and the accurate and rapid identification of the STN in PD rats was performed using detection sites distributed at different brain depths.These results showed that nuclei in 6-hydroxydopamine hydrobromide(6-OHDA)-lesioned brains discharged more intensely than those in unlesioned brains.In addition,the MEA simultaneously acquired neural signals from both the STN and the upper or lower boundary nuclei of the STN.Moreover,higher values of spike firing rate,spike amplitude,local field potential(LFP)power,and beta oscillations were detected in the STN of the 6-OHDA-lesioned brain,and may therefore be biomarkers of STN localization.Compared with the STNs of unlesioned brains,the power spectral density of spikes and LFPs synchronously decreased in the delta band and increased in the beta band of 6-OHDA-lesioned brains.This may be a cause of sleep and motor disorders associated with PD.Overall,this work describes a new cellular-level localization and detection method and provides a tool for future studies of deep brain nuclei.

基于UCA-FDA雷达距离补偿的DOA估计方法

主瓣欺骗式干扰给雷达在电子对抗中的应用带来了挑战。为了解决电子对抗中主瓣距离欺骗干扰的到达方向(direction of arrival,DOA)估计问题,基于均匀圆阵的频率分集阵列(uniform circular array-frequency diverse array,UCA-FDA)雷达,提出了一种利用距离补偿的DOA估计方法。所提方法通过对雷达接收信号进行距离补偿,消除距离维度上的影响,并将角度联合导向矢量和处理后的协方差矩阵代入多重信号分类(multiple signal classification,MUSIC)算法,从而获得目标的方位角和俯仰角。此外,所提方法还增加了分辨来自同一方向的多个目标的功能,具有角度估计精度高、抗干扰性能好、适用低快拍情景的优点。仿真实验也证明了所提方法的有效性。

Manipulating d-d orbital hybridization induced by Mo-doped Co_(9)S_(8) nanorod arrays for high-efficiency water electrolysis

Precisely refining the electronic structure of electrocatalysts represents a powerful approach to further optimize the electrocatalytic performance.Herein,we demonstrate an ingenious d-d orbital hybridization concept to construct Mo-doped Co_(9)S_(8) nanorod arrays aligned on carbon cloth(CC)substrate(abbreviated as Mo-Co_(9)S_(8)@CC hereafter)as a high-efficiency bifunctional electrocatalyst toward water electrolysis.It has experimentally and theoretically validated that the 4d-3d orbital coupling between Mo dopant and Co site can effectively optimize the H_(2)O activation energy and lower H^(*)adsorption energy barrier,thereby leading to enhanced hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)activities.Thanks to the unique electronic and geometrical advantages,the optimized Mo-Co_(9)S_(8)@CC with appropriate Mo content exhibits outstanding bifunctional performance in alkaline solution,with the overpotentials of 75 and 234 mV for the delivery of a current density of 10 mA cm^(-2),small Tafel slopes of 53.8 and 39.9 mV dec~(-1)and long-term stabilities for at least 32 and 30 h for HER and OER,respectively.More impressively,a water splitting electrolylzer assembled by the self-supported Mo-Co_(9)S_(8)@CC electrode requires a low cell voltage of 1.53 V at 10 mA cm^(-2)and shows excellent stability and splendid reversibility,demonstrating a huge potential for affordable and scalable electrochemical H_(2) production.The innovational orbital hybridization strategy for electronic regulation herein provides an inspirable avenue for developing progressive electrocatalysts toward new energy systems.

白藜芦醇调控LncRNA-UCA1/Wnt/β-catenin信号通路抑制非小细胞肺癌的机制研究

[目的]探讨白藜芦醇通过调控非小细胞肺癌细胞中LncRNA-UCA1的表达,进而影响Wnt/β-catenin信号通路,最后抑制肿瘤细胞的增殖、侵袭并促进凋亡等一系列生物学功能。[方法]首先探究白藜芦醇对非小细胞肺癌细胞系(A549和Hcc827)的影响,使用细胞活力检测(CCK-8)试剂盒检测细胞活力;Transwell小室和划痕实验验证肿瘤细胞的迁移和侵袭;流式细胞术检测肿瘤细胞的凋亡;Western Blot检测相关通路蛋白(Wnt/β-catenin)的表达情况。随后,应用微阵列技术筛选非小细胞肺癌细胞和正常人肺上皮细胞中异常表达的长链非编码RNA(LncRNA),通过LncRNA-尿路上皮癌抗原1(UCA1)过表达和白藜芦醇共同孵育A549细胞,检测其对肿瘤细胞增殖、迁移、侵袭和凋亡的影响,以及Wnt/β-catenin通路相关蛋白的变化。[结果]白藜芦醇可以呈剂量依赖性的抑制非小细胞肺癌细胞系(A549和Hcc827)的增殖和侵袭并且促进其凋亡,并且下调Wnt/β-catenin信号通路中的β-catenin、CyclinD1、c-myc蛋白表达;同时,LncRNA-UCA1在非小细胞肺癌细胞系中高表达,而白藜芦醇可显著抑制其表达;此外,过表达LncRNA-UCA1可通过调节Wnt/β-catenin信号通路促进非小肺癌细胞系(A549和Hcc827)的增殖,迁移和侵袭并抑制细胞凋亡,而加入白藜芦醇后可以显著抑制上述效应。[结论]LncRNA-UCA1在非小细胞肺癌细胞系中高表达,而白藜芦醇可以通过下调其表达水平,进而调控Wnt/β-catenin信号通路来抑制非小细胞肺癌细胞的增殖,迁移和侵袭并促进其凋亡。

广义Lucas序列的重复度与相关的不定方程的解数

用 p- adic分析方法讨论了广义 L ucas序列的重复度 ,并由此证明了不定方程 ax2 +D=cpn,x>0 ,a D>0 ,c=1,2 ,4,p为素数 ,除四种例外情形外 ,最多只有两组解 (x,n) .

UCA1在AML患者骨髓组织单个核细胞中的表达及对AML细胞增殖凋亡调节作用观察

目的观察长链非编码RNA(long non-coding RNAs,LncRNA)尿路上皮癌胚抗原(urothelial carcinoma associated gene 1,UCA1)在急性髓系白血病(AML)患者骨髓组织单个核细胞中的表达情况及对人AML不同亚型细胞株U937、HL60增殖凋亡的调节作用,并探讨其可能作用机制。方法(1)30例AML患者、30例缺铁性贫血患者的骨髓标本,采用RT-PCR检测骨髓组织单个核细胞UCA1。(2)将U937及HL60细胞各分为1、2、3组。1、2组分别用UCA1-siRNA(可抑制UCA1表达)和空载siRNA转染,3组为空白对照(不作任何处理)。分别于转染24、48、72 h取各组细胞,MTT法测算细胞增殖活力。转染48 h时采用流式细胞术观察各组细胞凋亡情况。转染48 h时采用WESTERN Blotting法检测各组细胞Wnt、β-连环蛋白(β-catenin)、p-gsk-3?蛋白。结果AML及缺铁性贫血患者骨髓组织单个核细胞UCA1相对表达量分别为4.96±1.51、1.09±0.93,二者比较,P<0.05。与2、3组比较,转染24、48、72 h时U937及HL-601组细胞增殖活力低(P均<0.05)。U937细胞1、2、3组细胞凋亡率分别为34.20%±1.21%、7.21%±1.41%、6.84%±1.53%,HL-60细胞1、2、3组细胞凋亡率分别为34.21%±1.83%、7.20%±1.56%、7.85%±1.93%,与2、3组比较,U937及HL-60细胞1组细胞凋亡率高(P均<0.05)。与2、3组比较,U937及HL-601组细胞Wnt、β-catenin相对表达量降低,p-gsk-3?蛋白相对表达量升高(P均<0.05)。结论与缺铁性贫血患者比较,AML患者骨髓组织单个核细胞UCA1表达升高。抑制UCA1表达可抑制U937及HL-60细胞的增殖,促进细胞凋亡,其机制可能与UCA1抑制细胞Wnt/β-catenin信号通路有关。

Biomechanical Considerations in the Unruptured Cerebral Aneurysm Study (UCAS Japan): Rupture Risk and True Stress of Wall

When an unruptured aneurysm is found, deciding whether to operate or follow up is one of the most important issues. There are guidelines for making the best final decision on treatment, taking into account the effectiveness of diagnostic and therapeutic devices and the risk-benefit ratio of patients, caregivers, and healthcare professionals. The guidelines evidence-based of large clinical data for this purpose are presented by national medical societies. As one of the rupture risk indicators, there is the hazard risk ratio derived by the UCAS Japan research group based on the statistical method of 6697 aneurysms in 5720 patients with cerebral aneurysms of 3 mm or more. Therefore, we investigated the biomechanical significance of this hazard risk ratio using a spherical aneurysm model. It was revealed that 1) the reason why the frequency of aneurysm rupture is relatively high up to about 10 mm, 2) the UCAS hazard risk ratio corresponds to stress of the aneurysm wall, and the true stress can be calculated by multiplying the patient’s blood pressure, and 3) the factors that cause the daughter’s sac (irregular protrusion of the aneurysm wall). In addition, our two methods for measuring the strength of the blood vessel wall of an individual patient were described.

一般情形下的UCA辐射方向图理论研究

产生涡旋波束的方法有多种,其中最常见的是均匀圆阵列(UCA),具有简单的结构和便捷的模式调控能力。然而,在高阶模态下,增加UCA的单元数会导致成本和空间占比增大,并且可能降低高阶模态的纯度。目前,UCA的设计多种多样,但对于选择合适单元数量的研究还比较缺乏。本文基于DFT,分析UCA的辐射方向图,并在偶数单元数的基础上增加奇数单元数,同时考虑了阵列半径、观测面与圆阵的距离以及观测圆的半径这些因素对纯度的影响。通过仿真研究设定了纯度阈值,从而确定最适合的单元数量。UCA实验结果表明,N=3时带内隔离度可达到35dB,而N=4保持在28-35dB,因此奇数单元有较稳定的隔离度。此外内环N=3,外环N=5的同心圆环阵列(UUCA)的隔离度均大于20dB。综上,在选择UCA单元数量时需要综合考虑纯度要求、成本、空间限制和系统性能等因素。

益气养阴通络方通过lncRNA UCA1靶向调控miR-485-5p抑制糖尿病肾病大鼠肾小管上皮细胞凋亡及炎症反应作用机制

目的:探讨益气养阴通络方通过lncRNA UCA1靶向调控miR-485-5p抑制糖尿病肾病(DN)大鼠肾小管上皮细胞凋亡和炎症反应作用机制。方法:选取雄性Wistar大鼠50只,随机分为空白组、模型组和益气养阴通络方低、中、高剂量组,每组各10只;除空白组外,其余组大鼠采用高糖高脂胆固醇饮食造模。检测各组大鼠肾功能指标和炎症因子水平;采用流式细胞术检测各组大鼠细胞凋亡率;WB检测各组大鼠细胞凋亡蛋白表达;PCR检测大鼠UCA1、miR-485-5p mRNA水平;采用双荧光素酶报告基因实验检测UCA1与miR-485-5p的结合位点。结果:与模型组比较,益气养阴通络方不同剂量组24 h尿蛋白(24 hUTP)、血清肌酐(SCr)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平较低,且呈剂量依赖性(均P<0.05);与模型组比较,益气养阴通络方不同剂量组大鼠细胞凋亡率较低,且呈剂量依赖性(均P<0.05);与模型组比较,益气养阴通络方不同剂量组NLRP3、Caspase-1蛋白表达量较低,且呈剂量依赖性(均P<0.05);与模型组比较,益气养阴通络方不同剂量组UCA1 mRNA水平较高,miR-485-5p mRNA水平较低,且呈剂量依赖性(均P<0.05);UCA1与miR-485-5p存在结合位点,具有靶向调控关系。结论:益气养阴通络方可能通过lncRNA UCA1靶向调控miR-485-5p抑制DN大鼠肾小管上皮细胞凋亡和炎症反应。

A core-shell copper oxides-cobalt oxides heterostructure nanowire arrays for nitrate reduction to ammonia with high yield rate

Electrochemical nitrate reduction to ammonia(NRA) can realize the green synthesis of ammonia(NH3) at ambient conditions, and also remove nitrate contamination in water. However, the current catalysts for NRA still face relatively low NH3yield rate and poor stability. We present here a core-shell heterostructure comprising cobalt oxide anchored on copper oxide nanowire arrays(CuO NWAs@Co_(3)O_(4)) for efficient NRA. The CuO NWAs@Co_(3)O_(4)demonstrates significantly enhanced NRA performance in alkaline media in comparison with plain CuO NWAs and Co_(3)O_(4)flocs. Especially, at-0.23 V vs. RHE, NH_(3) yield rate of the CuO NWAs@Co_(3)O_(4)reaches 1.915 mmol h^(-1)cm^(-2),much higher than those of CuO NWAs(1.472 mmol h^(-1)cm^(-2)), Co_(3)O_(4)flocs(1.222 mmol h^(-1)cm^(-2)) and recent reported Cu-based catalysts.It is proposed that the synergetic effects of the heterostructure combing atom hydrogen adsorption and nitrate reduction lead to the enhanced NRA performance.

长链非编码RNA UCA1促进人骨髓间充质干细胞成骨分化研究

目的探讨lncRNA UCA1对人骨髓间充质干细胞成骨分化的影响。方法构建过表达lncRNA UCA1的慢病毒载体(UCA1a和UCA1b)和空载体分别感染人骨髓间充质干细胞。实验分为4组:对照组1为干细胞对照组;对照组2为空载体对照组;UCA1a为高表达UCA1a的实验组;UCA1b为高表达UCA1b的实验组。在成骨诱导分化7 d、14 d、21 d时,进行茜素红染色。采用Real-Time RT-PCR检测成骨相关基因Col1、ALP、OPN和SP7的mRNA表达水平。结果表达UCA1a和UCA1b上调hBMSCs成骨相关基因。在7 d时Col1表达水平高于对照组。14 d时hBMSCs的成骨相关基因ALP、Col1、OPN和SP7的mRNA水平均高于对照组。21 d时,ALP的表达水平上调,P<0.05为差异有统计学意义。两对照组间无显著差异。结论表达两种结构的UCA1体外均促进hBMSCs的成骨分化能力,未来有望基于UCA1调控hBMSCs成骨分化,用于修复颌面部骨缺损。

通过UCAS——转学英国

一流的教育质量和安全的居住环境使英国成了留学圣地，每年有近十万留学生从世界各地涌到英国求学，其中一部分学生是通过转学到英国留学的，并且直接插班攻读英国本科学位。也许您正在为求学的事情苦恼，那么请您仔细阅读本篇文章，你将得到一个比较明确的答案。

Bidirectional visible light absorber based on nanodisk arrays

A perfect bidirectional broadband visible light absorber composed of titanium nitride and tungsten nanodisk arrays is proposed.The average absorption of the absorber exceeds 89%at 400 nm–800 nm when light is normally incident on the front-side.Illumination from the opposite direction(back-side)results in absorption of more than 75%.Through the theoretical analysis of the electric and magnetic fields,the physical mechanism of the broadband perfect absorption is attributed to the synergy of localized surface plasmons,propagating surface plasmons,and plasmonic resonant cavity modes.Furthermore,the absorber also exhibits excellent polarization-independence performance and a high angular tolerance of~30°for both front-and back-side incidence.The designed bidirectional broadband visible light absorber here has wide application prospects in the fields of solar cells and ink-free printing.

Controllable and large-area growth of ZnO nanosheet arrays under ambient condition as superior anodes for scalable aqueous batteries

Two-dimensional(2D)oxides have been the focus of substantial research interest recently,owing to their fascinating physico-chemical properties.However,fabrication of large-area 2D oxide materials in a controlled manner under mild conditions still remains a formidable challenge.Herein,we develop a facile and universal strategy based on the sonochemistry approach for controllable and large-area growth of quasi-aligned single-crystalline ZnO nanosheets on a Zn substrate(Zn@SC-ZnO)under ambient conditions.The obtained ZnO nanosheets possess the desired exclusively exposed(001)facets,which have been confirmed to play a critical role in significantly reducing the activation energy and facilitating the stripping/plating processes of Zn.Accordingly,the constructed Zn@SC-ZnO||Zn@SC-ZnO symmetric cell has very low polarization overpotential down to~20 mV,with limited dendrite growth and side reactions for Zn anodes.The developed Zn@SC-ZnO//MnO_(2)aqueous Zn-ion batteries(ZIBs)show a voltage efficiency of 88.2%under 500 mA g^(-1)at the stage of 50%depth of discharge,which is state of the art for ZIBs reported to date.Furthermore,the as-assembled large-size cell(5 cm×5 cm)delivers an open circuit potential of 1.648 V,and can be robustly operated under a high current of 20 mA,showing excellent potential for future scalable applications.

Homogenous metallic deposition regulated by abundant lithiophilic sites in nickel/cobalt oxides nanoneedle arrays for lithium metal batteries

Although lithium(Li)metal delivers the highest theoretical capacity as a battery anode,its high reactivity can generate Li dendrites and"dead"Li during cycling,resulting in poor reversibility and low Li utilization.Inducing uniform Li plating/stripping is the core of solving these problems.Herein,we design a highly lithiophilic carbon film with an outer sheath of the nanoneedle arrays to induce homogeneous Li plating/stripping.The excellent conductivity and 3D framework of the carbon film not only offer fast charge transport across the entire electrode but also mitigate the volume change of Li metal during cycling.The abundant lithiophilic sites ensure stable Li plating/stripping,thereby inhibiting the Li dendritic growth and"dead"Li formation.The resulting composite anode allows for stable Li stripping/plating under 0.5 mA cm^(-2) with a capacity of 0.5 mA h cm^(-2) for 4000 h and 3 mA cm^(-2) with a capacity of3 mA h cm^(-2) for 1000 h.The Ex-SEM analysis reveals that lithiophilic property is different at the bottom,top,or channel in the structu re,which can regulate a bottom-up uniform Li deposition behavior.Full cells paired with LFP show a stable capacity of 155 mA h g^(-1) under a current density of 0.5C.The pouch cell can keep powering light-emitting diode even under 180°bending,suggesting its good flexibility and great practical applications.

Developing Three-Dimensional Beamshaping Techniques on Volumetric Random Arrays to Suppress Array Sidelobes

This paper proposes a three-dimensional (3-D) amplitude tapering technique on volumetric random arrays to minimize array sidelobes and emulate phased array operations on mobile platforms. Our ultimate goal is to realize wireless phased array applications carried out by mobile platforms;in this paper, we focus on the development of collaborative beamforming algorithms. This beamshaping technique mitigates the discontinuity of the current distribution along the array aperture and lower array sidelobe level (SLL) by specially paying attention to the array element’s depth deviation. In this work, step by step amplitude tapering procedures are clearly illustrated. Further, a reconfigurable phased array with sixteen patch antennas is tested to verify the fidelity of the 3-D beamshaping algorithm. Measured and simulated radiation patterns are benchmarked to evaluate the sidelobe suppression results, and the best sidelobe suppressed region is around the array’s main beam.

Shear wave splitting analysis of local earthquakes from dense arrays in Shimian,Sichuan

The Shimian area of Sichuan sits at the junction of the Bayan Har block.Sichuan-Yunnan rhombic block,and Yangtze block,where several faults intersect.This region features intense tectonic activity and frequent earthquakes.In this study,we used local seismic waveform data recorded using dense arrays deployed in the Shimian area to obtain the shear wave splitting parameters at 55 seismic stations and thereby determine the crustal anisotropic characteristics of the region.We then analyzed the crustal stress pattern and tectonic setting and explored their relationship in the study area.Although some stations returned a polarization direction of NNW-SSE.a dominant polarization direction of NW-SE was obtained for the fast shear wave at most seismic stations in the study area.The polarization directions of the fast shear wave were highly consistent throughout the study-area.This orientation was in accordance with the direction of the regional principal compressive stress and parallel to the trend of the Xianshuihe and Daliangshan faults.The distribution of crustal anisotropy in this area was affected by the regional tectonic stress field and the fault structures.The mean delay time between fast and slow shear waves was 3.83 ms/km.slightly greater than the values obtained in other regions of Sichuan.This indicates that the crustal media in our study area had a high anisotropic strength and also reveals the influence of tectonic complexity resulting from the intersection of multiple faults on the strength of seismic anisotropy.