Inulin is a prebiotic dietary fiber that plays an integral role in producing functional dairy products with improved health benefits. Therefore, the objectives of this study are as follows: extract and purify inulin f...Inulin is a prebiotic dietary fiber that plays an integral role in producing functional dairy products with improved health benefits. Therefore, the objectives of this study are as follows: extract and purify inulin from chicory roots and globe artichoke roots;evaluate the physicochemical, functional properties and functional groups of the purified inulin;determine the functional properties of chicory roots inulin-maltodextrin and globe artichoke roots inulin-maltodextrin and compare it with that of the commercial inulin;examine the impact of various inulin on physiochemical, microstructural, textural, sensory characteristics and as prebiotic dietary fiber on probiotic bacteria’s viability of stirred bio-yogurt. The characteristics of the microstructure were investigated by scanning electron microscopy and, Fourier transforms infrared spectroscopy to detect the functional group. The resulting inulin exhibited a high yield and purity along with enhanced functional properties. Stirred bio-yogurt fortified with chicory roots inulin or globe artichoke roots inulin showed enhanced physicochemical, microstructural, microbiological, and overall sensorial acceptability followed by chicory roots inulin-maltodextrin or globe artichoke roots inulin-maltodextrin and the commercial inulin as compared to the control. Stirred bio-yogurt samples can offer various health benefits and wide applications as supplement of prebiotic dietary fiber in dairy industry.展开更多
Objective Chlorination is often used to disinfect recreational water in large amusement parks;however,the health hazards of chlorination disinfection by-products(DBPs)to occupational populations are unknown.This study...Objective Chlorination is often used to disinfect recreational water in large amusement parks;however,the health hazards of chlorination disinfection by-products(DBPs)to occupational populations are unknown.This study aimed to assess the exposure status of chlorinated DBPs in recreational water and the health risks to employees of large amusement parks.Methods Exposure parameters of employees of three large amusement parks in Shanghai were investigated using a questionnaire.Seven typical chlorinated DBPs in recreational water and spray samples were quantified by gas chromatography,and the health risks to amusement park employees exposed to chlorinated DBPs were evaluated according to the WHO's risk assessment framework.Results Trichloroacetic acid,dibromochloromethane,bromodichloromethane,and dichloroacetic acid were detected predominantly in recreational water.The carcinogenic and non-carcinogenic risks of the five DBPs did not exceed the risk thresholds.In addition,the carcinogenic and non-carcinogenic risks of mixed exposure to DBPs were within the acceptable risk limits.Conclusion Typical DBPs were widely detected in recreational water collected from three large amusement parks in Shanghai;however,the health risks of DBPs and their mixtures were within acceptable limits.展开更多
Ensuring the health and safety of drinking water is crucial for both nations and their citizens.Since the 20th century,the disinfection of drinking water,effectively controlling pathogens in water sources,has become o...Ensuring the health and safety of drinking water is crucial for both nations and their citizens.Since the 20th century,the disinfection of drinking water,effectively controlling pathogens in water sources,has become one of the significant advances in public health.However,the disinfectants used in the process,such as chlorine and chlorine dioxide,react with natural organic matter in the water to produce disinfection by-products(DBPs).Most of these DBPs contain chlorine,and if the source water contains bromine or iodine,brominated or iodinated DBPs,collectively referred to as Halogenated disinfection byproducts(X-DBPs),are formed.Numerous studies have found that X-DBPs pose potential risks to human health and the environment,leading to widespread concern.Mass spectrometry has become an important means of discovering new types of X-DBPs.This paper focuses on the study of methods for analyzing X-DBPs in drinking water using mass spectrometry.展开更多
The ameliorative effect of external Ca^2+ on Jerusalem artichoke (Helianthus tuberosus L.) under salt stress was studied through biochemical and physiological analyses of Jerusalem artichoke seedlings treated with ...The ameliorative effect of external Ca^2+ on Jerusalem artichoke (Helianthus tuberosus L.) under salt stress was studied through biochemical and physiological analyses of Jerusalem artichoke seedlings treated with or without 10 mol L^-1 CaCl2, 150 mmol L^-1 NaCl, and/or 5 mmol L^-1 ethylene-bis(oxyethylenenitrilo)-tetraacetic acid (EGTA) for five days. Exposure to NaC1 (150 mmol L^-1) decreased growth, leaf chlorophyll content, and photosynthetic rate of Jerusalem artichoke seedlings. NaC1 treatment showed 59% and 37% higher lipid peroxidation and electrolyte leakage, respectively, than the control. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were decreased by NaCl, indicating an impeded antioxidant defense mechanism of Jerusalem artichoke grown under salt stress. Addition of 10 mmol L^-1 CaCl2 to the salt solutions significantly decreased the damaging effect of NaC1 on growth and chlorophyll content and simultaneously restored the rate of photosynthesis almost to the level of the control. Ca^2+ addition decreased the leaf malondialdehyde (MDA) content and electrolyte leakage from NaCl-treated seedlings by 47% and 24%, respectively, and significantly improved the activities of SOD, POD, and CAT in NaCl-treated plants. Addition of EGTA, a specific chelator of Ca2+, decreased the growth, chlorophyll content, and photosynthesis, and increased level of MDA and electrolyte leakage from NaCl-treated plants and from the control plants. EGTA addition to the growth medium also repressed the activities of SOD, POD, and CAT in NaCl-treated and control seedlings. External Ca2+ might protect Jerusalem artichoke against NaC1 stress by up-regulating the activities of antioxidant enzymes and thereby decreasing the oxidative stress.展开更多
Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement, but little effort has been made to characterize its genetic resources. A study was condu...Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement, but little effort has been made to characterize its genetic resources. A study was conducted to assess genetic structure and genetic relatedness of 47 diverse Jerusalem artichoke accessions using RAPD, ISSR and SRAP markers. A total of 296 (87.1%) polymorphic bands were detected from 13 RAPD markers;92 (80%) from six ISSR primers;and 194 (88.6%) for nine combinations of SRAP primers. Five optimal clusters were inferred by the STRUCTURE program from the RAPD or ISSR data, while six optimal clusters were found from the SRAP data or combined marker data. Significant linear relationships between the distance matrices for all pairs of individual accessions were detected for all marker pairs and the estimated correlation coefficient was 0.40 for RAPD-ISSR, 0.53 for RAPD-SRAP, and 0.43 for ISSR-SRAP. Based on the combined data, the neighbor-joining clustering of the 47 accessions matched closely with those inferred from the STRUCTURE program. Three ancestral groups were observed for the Canadian germplasm. Most diverse germplasm harbored in the USA collection. These findings not only reveal the compatible patterns of genetic structure and relatedness inferred with three marker types, but also are useful for managing Jerusalem artichoke germplasm and utilizing diverse germplasm for genetic improvement.展开更多
The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol,an important chemical ...The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol,an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae.In this study,we investigated the use of salting-out extraction(SOE) that employed a K2HPO4/ethanol system consisting of 21% ethanol and 17% K2HPO4(mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE,about 98% of solid matters was removed,and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%,respectively,and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.展开更多
Irrigation with various dilutions of seawater can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture in the coastal semi-arid areas of t...Irrigation with various dilutions of seawater can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture in the coastal semi-arid areas of the North China Plain. Jerusalem artichoke (Helianthus tuberosus L.) grown in a field experiment was irrigated with seawater diluted with freshwater from 2001 to 2003 to determine the feasibility of seawater irrigation in the Laizhou area. For treatments of CK (non-irrigation) along with seawater concentrations of 25%, 50%, and 75%, total dissolved solid (TDS) in the non-irrigated soil significantly increased (P ≤ 0.05) in both 2002 and 2003 and was 1.3 times higher in 2003 than in 2001. In the 25% and 50% seawater concentration treatments, TDS in 2001 was significantly greater (P ≤ 0.05) than CK; however, TDS in these two treatments decreased by 34.9% and 40.1%, respectively, in 2003 compared with 2001. The sodium adsorption ratio (SAR) remained below 10 mmol^1/2 L^-1/2, indicating that alkalization was low with seawater irrigation. In 2001 and 2002, compared to CK and the irrigation treatment with 75% seawater, irrigation with 25% and 50% seawater increased the yields of Jerusalem .artichoke. This meant that Jerusalem artichoke could be safely grown in salt-affected land of Laizhou area with 25% and 50% seawater irrigation.展开更多
Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the effects ...Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the effects of processing variables (extraction temperature, pH, extraction time, and liquid-to- solid ratio) on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions: pHl .52, 63.62 min, 100℃ and a liquid-to-solid ratio of 44.4 mL/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52+0.90% was obtained, which validated the model prediction. The effects of different drying methods (freeze drying, spray drying and vacuum drying) on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural differences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by different methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin (SDP) and apple pectin (AP) were stronger than those of vacuum oven dried pectin (ODP) and vacuum freeze dried pectin (FDP). Therefore compared with the other two drying methods, the spray drying method was the best.展开更多
An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) ...An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.展开更多
Two varieties of Jerusalem artichoke (early maturing variety Qingyu No.1 and middle maturing variety Qingyu No.2),which were bred independently by Research and Development Center of Jerusalem Artichoke,Qinghai Academy...Two varieties of Jerusalem artichoke (early maturing variety Qingyu No.1 and middle maturing variety Qingyu No.2),which were bred independently by Research and Development Center of Jerusalem Artichoke,Qinghai Academy of Agriculture and Forestry,were used as materials,and the content and dynamic changes of three kinds of carbohydrates (sucrose,glucose and fructose) in various organs of Jerusalem artichoke in different periods in two natural habitats (water plain and low hill dry land) were studied.The results showed that the content of the three kinds of carbohydrates in Qingyu No.1 and Qingyu No.2 in the water plain and low hill dry land was relatively high in late June and late August.The total content of the three carbohydrates in Qingyu No.1 peaked earlier than that of Qingyu No.2.The total content of the three carbohydrates in the water plain peaked earlier than that of the low hill dry land.In the whole growth period,the total content of the three carbohydrates in the stems and roots of Jerusalem artichoke was higher than that of the leaves.In the leaves,fructose accumulation was relatively obvious in the whole growth period,and the content was high,followed by glucose.In the stems and roots,fructose content was high,followed by sucrose.In the tubers,glucose accumulation was obvious,and sucrose content was lower than glucose and fructose content.When the tubers were harvested,fructose content was high.展开更多
Inulin, an oligosaccharide produced by several plants, has been shown to enhance the viability of probiotic cultures in milk through storage. Jerusalem artichoke ( Helianthus tuberosus L.) is an interested prebiotic b...Inulin, an oligosaccharide produced by several plants, has been shown to enhance the viability of probiotic cultures in milk through storage. Jerusalem artichoke ( Helianthus tuberosus L.) is an interested prebiotic because its tuber has risen content of inulin and fructo-oligosaccharides. This study was aimed to: 1) set the effect of Jerusalem artichoke in deferent concentrations (2.5% & 5%) on the growth of probiotic Lb. acidophilus P106 in the bio-yoghurt during cold storage at 5℃ and sensory evaluation of probiotic yoghurts;2) study the effect of feeding with this synbiotic fermented milk on diabetic mice. It could be concluded that the Jerusalem artichoke influenced the growth of Lb. acidophilus P106 and 5% (w/v) Jerusalem artichoke was given the highest growth and sensory evaluation. On the other hand, no serious adverse effects were observed;the reduction of blood glucose was observed at the termination of empirical phase, also, high level (5%) of Jerusalem artichoke led to more reduction of blood glucose, cholesterol levels and total lipids compared with control.展开更多
Biochemical characteristics, antimicrobial and quorum quenching activity of the extract of the “Carciofo di Montoro”, a typical ecotype of Cynara cardunculus var. scolymus of the Campania region (Southern Italy) wer...Biochemical characteristics, antimicrobial and quorum quenching activity of the extract of the “Carciofo di Montoro”, a typical ecotype of Cynara cardunculus var. scolymus of the Campania region (Southern Italy) were studied, to consider it as potential reserve of bioactive constituents useful for food industry and beneficial for managing and preventing several chronic illnesses in humans. The extract exhibited a good polyphenol content (528 μg GAE/g) and antioxidant activity (EC50 less than 5 mg). Ultra pressure liquid chromatography (UPLC) revealed high amount of chlorogenic acid, cynarin and epicatechin. The extract showed antimicrobial activity against Escherichia coli, Staphylocccus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus pathogen strains. Finally, quorum quenching activity was demonstrated. The variety Carciofo di Montoro could represent a good source of health-promoting polyphenols, encouraging a nutraceutical use of such ecotype, for several phyto-pharmaceutical applications.展开更多
The dynamics of biomass accumulation during the growing period, the yield of leafy stalks and tubers, and the nutrient concentration and nutrient uptake of the yield were investigated for two Jerusalem artichoke varie...The dynamics of biomass accumulation during the growing period, the yield of leafy stalks and tubers, and the nutrient concentration and nutrient uptake of the yield were investigated for two Jerusalem artichoke varieties (Tápiói Korai and Tápiói Sima) in a field experiment involving mineral fertilisation. Considerable differences were observed between the dynamics of leafy stalk and tuber development in Tápiói Korai which has a short vegetation period and Tápiói Sima where the vegetation period is long. The maximum dry matter ratio between the tuber yield and the leafy stalk yield was 1:1 for Tápiói Korai and 1:4.5 for Tápiói Sima. During the period when the maximum aboveground biomass developed in Tápiói Korai, 100 kg.ha-1 N and P fertiliser resulted in the highest leafy stalk yield (38.34 t.ha-1), while for Tápiói Sima, which developed a much greater leafy stalk mass, the highest aboveground biomass yield (78-80 t.ha-1) was given in response to 200 kg.ha-1 N supplemented by P and K fertiliser. Both artichoke varieties produced the great-est tuber yield at a N rate of 200 kg.ha-1, supplemented with P and K fertiliser. The nutrient concentration in the leafy stalks was highest on the 85th day of the vegetation period, prior to intensive dry matter accumulation in the leafy stalks and before tuber formation began. In both varieties the maximum nutrient uptake was recorded on the 155th day. Great differences were observed between the varieties in terms of specific nutrient uptake. For a tuber yield of 10 t, together with the corresponding leafy stalk yield, the specific nutrient uptake of the Tápiói Korai variety amounted to 48 kg N, 10 kg P, 83 kg K, 30 kg Ca and 10 kg Mg, while for Tápiói Sima these figures were 162 kg N, 30 kg P, 300 kg K, 84 kg Ca and 45 kg Mg.展开更多
Fructo-oligosaccharide (FOS) is a type of dietary fiber. It can not be digested by enzymes in the body. It also helps to improve a digestion in the intestine, an excretion system and prevent the cancer. Jerusalem ar...Fructo-oligosaccharide (FOS) is a type of dietary fiber. It can not be digested by enzymes in the body. It also helps to improve a digestion in the intestine, an excretion system and prevent the cancer. Jerusalem artichoke is scanned and analyzed by High Performance Liquid Chromatography (HPLC) for quantity of FOS. They are selected for extraction and drying process. Studies on the extraction for a powder making, the conditions are optimum for maximum yield. Experiments manage using a factorial real 2 × 4 × 6 in 2 blocks of Randomized Complete Block Design (RCBD) models. The first factor studies on two types of solvent. The second factor is the four levels of temperature in the extraction with water at 25 ℃, 35 ℃, 50℃ and 60℃ for 30 min. The third factor is the ratio of sample per solvent as 5, 6, 10, 15, 20 and 30 times. The data and comparison of average is analyzed by Duncan's New Mutiple Range Test at the significant level 0.05. The concentrated extracts are processed to powder by freeze drying, a hot air and vacuum drying. Optimal conditions control the temperature and time by heating in both vacuum and conventional oven. The results of temperature on the extraction are significantly different at a = 0.05. In drying process with high temperature, the color of product is more yellow (b) and less white (L). The difference of color value is statistically significant at level a = 0.05. The sensory evaluation of food products are added the extracted powder and attribute by panelist. High level acceptance of product is aspectual about texture and flavor. FOS is the trend of functional foods for health. It is focus on low energy and on obesity including increasing the absorption of calcium.展开更多
Strains K.marxianus Y-303,Oenoferm credo,Oenoferm rouge,Sacch.vini Rkaseteli-6,Sacch.cerevisiae XII and Sacch.uvarum 73 were used for fermentation of juice,extracted from Jerusalem artichoke tubers grown in Uzbekistan...Strains K.marxianus Y-303,Oenoferm credo,Oenoferm rouge,Sacch.vini Rkaseteli-6,Sacch.cerevisiae XII and Sacch.uvarum 73 were used for fermentation of juice,extracted from Jerusalem artichoke tubers grown in Uzbekistan.The fermentation process was carried out in laboratory conditions.Among these yeasts Oenoferm credo,Oenoferm rouge and Sacch.uvarum 73 showed higher performance than K.marxianus Y-303 and the ethanol yields were 92%,90%and 88%,respectively.After 72 h fermentation with Sacch.vini Rkaseteli-6 and Sacch.cerevisiae XII the yield of ethanol was 82%and 74%of maximum theoretical yield,respectively.展开更多
Jerusalem artichoke has been cultivated in various regions without any special breeding technique. As a food, Jerusalem artichoke has a characteristic flavour and functional ingredients including inulin, other dietary...Jerusalem artichoke has been cultivated in various regions without any special breeding technique. As a food, Jerusalem artichoke has a characteristic flavour and functional ingredients including inulin, other dietary fibers, minerals and traces of polyphenol. The production of chips from Jerusalem artichoke was examined in this study because it contains high amount of inulin. Inulin gives health promoting effects especially for the people suffering from diabetes. Firstly,chips production from Jerusalem artichoke was studied in the scope of properties like moisture, oil, color, texture, sensory. After cleaning, tubers prepared in specified size and slices were cooked in the deep fat fryer or microwave oven. The best results obtained for frying and microwave oven application of Jerusalem artichoke were obtained at 180℃ for 240s treatment time and at 600 W for105s, respectively.展开更多
文摘Inulin is a prebiotic dietary fiber that plays an integral role in producing functional dairy products with improved health benefits. Therefore, the objectives of this study are as follows: extract and purify inulin from chicory roots and globe artichoke roots;evaluate the physicochemical, functional properties and functional groups of the purified inulin;determine the functional properties of chicory roots inulin-maltodextrin and globe artichoke roots inulin-maltodextrin and compare it with that of the commercial inulin;examine the impact of various inulin on physiochemical, microstructural, textural, sensory characteristics and as prebiotic dietary fiber on probiotic bacteria’s viability of stirred bio-yogurt. The characteristics of the microstructure were investigated by scanning electron microscopy and, Fourier transforms infrared spectroscopy to detect the functional group. The resulting inulin exhibited a high yield and purity along with enhanced functional properties. Stirred bio-yogurt fortified with chicory roots inulin or globe artichoke roots inulin showed enhanced physicochemical, microstructural, microbiological, and overall sensorial acceptability followed by chicory roots inulin-maltodextrin or globe artichoke roots inulin-maltodextrin and the commercial inulin as compared to the control. Stirred bio-yogurt samples can offer various health benefits and wide applications as supplement of prebiotic dietary fiber in dairy industry.
基金funded by the Foundation of State Environmental Protection Key Laboratory of Environmental Health Impact Assessment of Emerging Contaminants(Grant No.SEPKL-EHIAEC-202210)the Foundation of Shanghai Municipal Health Commission(Grant No.202240327)the Key Discipline Project of the Three-year Action Plan for Strengthening Public Health System Construction in Shanghai(2023-2025)(Grant No.GWVI-11.1-38)。
文摘Objective Chlorination is often used to disinfect recreational water in large amusement parks;however,the health hazards of chlorination disinfection by-products(DBPs)to occupational populations are unknown.This study aimed to assess the exposure status of chlorinated DBPs in recreational water and the health risks to employees of large amusement parks.Methods Exposure parameters of employees of three large amusement parks in Shanghai were investigated using a questionnaire.Seven typical chlorinated DBPs in recreational water and spray samples were quantified by gas chromatography,and the health risks to amusement park employees exposed to chlorinated DBPs were evaluated according to the WHO's risk assessment framework.Results Trichloroacetic acid,dibromochloromethane,bromodichloromethane,and dichloroacetic acid were detected predominantly in recreational water.The carcinogenic and non-carcinogenic risks of the five DBPs did not exceed the risk thresholds.In addition,the carcinogenic and non-carcinogenic risks of mixed exposure to DBPs were within the acceptable risk limits.Conclusion Typical DBPs were widely detected in recreational water collected from three large amusement parks in Shanghai;however,the health risks of DBPs and their mixtures were within acceptable limits.
文摘Ensuring the health and safety of drinking water is crucial for both nations and their citizens.Since the 20th century,the disinfection of drinking water,effectively controlling pathogens in water sources,has become one of the significant advances in public health.However,the disinfectants used in the process,such as chlorine and chlorine dioxide,react with natural organic matter in the water to produce disinfection by-products(DBPs).Most of these DBPs contain chlorine,and if the source water contains bromine or iodine,brominated or iodinated DBPs,collectively referred to as Halogenated disinfection byproducts(X-DBPs),are formed.Numerous studies have found that X-DBPs pose potential risks to human health and the environment,leading to widespread concern.Mass spectrometry has become an important means of discovering new types of X-DBPs.This paper focuses on the study of methods for analyzing X-DBPs in drinking water using mass spectrometry.
基金the National High Technology Research and Development Program of China (863 Program)(No. 2003AA627040).
文摘The ameliorative effect of external Ca^2+ on Jerusalem artichoke (Helianthus tuberosus L.) under salt stress was studied through biochemical and physiological analyses of Jerusalem artichoke seedlings treated with or without 10 mol L^-1 CaCl2, 150 mmol L^-1 NaCl, and/or 5 mmol L^-1 ethylene-bis(oxyethylenenitrilo)-tetraacetic acid (EGTA) for five days. Exposure to NaC1 (150 mmol L^-1) decreased growth, leaf chlorophyll content, and photosynthetic rate of Jerusalem artichoke seedlings. NaC1 treatment showed 59% and 37% higher lipid peroxidation and electrolyte leakage, respectively, than the control. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were decreased by NaCl, indicating an impeded antioxidant defense mechanism of Jerusalem artichoke grown under salt stress. Addition of 10 mmol L^-1 CaCl2 to the salt solutions significantly decreased the damaging effect of NaC1 on growth and chlorophyll content and simultaneously restored the rate of photosynthesis almost to the level of the control. Ca^2+ addition decreased the leaf malondialdehyde (MDA) content and electrolyte leakage from NaCl-treated seedlings by 47% and 24%, respectively, and significantly improved the activities of SOD, POD, and CAT in NaCl-treated plants. Addition of EGTA, a specific chelator of Ca2+, decreased the growth, chlorophyll content, and photosynthesis, and increased level of MDA and electrolyte leakage from NaCl-treated plants and from the control plants. EGTA addition to the growth medium also repressed the activities of SOD, POD, and CAT in NaCl-treated and control seedlings. External Ca2+ might protect Jerusalem artichoke against NaC1 stress by up-regulating the activities of antioxidant enzymes and thereby decreasing the oxidative stress.
文摘Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement, but little effort has been made to characterize its genetic resources. A study was conducted to assess genetic structure and genetic relatedness of 47 diverse Jerusalem artichoke accessions using RAPD, ISSR and SRAP markers. A total of 296 (87.1%) polymorphic bands were detected from 13 RAPD markers;92 (80%) from six ISSR primers;and 194 (88.6%) for nine combinations of SRAP primers. Five optimal clusters were inferred by the STRUCTURE program from the RAPD or ISSR data, while six optimal clusters were found from the SRAP data or combined marker data. Significant linear relationships between the distance matrices for all pairs of individual accessions were detected for all marker pairs and the estimated correlation coefficient was 0.40 for RAPD-ISSR, 0.53 for RAPD-SRAP, and 0.43 for ISSR-SRAP. Based on the combined data, the neighbor-joining clustering of the 47 accessions matched closely with those inferred from the STRUCTURE program. Three ancestral groups were observed for the Canadian germplasm. Most diverse germplasm harbored in the USA collection. These findings not only reveal the compatible patterns of genetic structure and relatedness inferred with three marker types, but also are useful for managing Jerusalem artichoke germplasm and utilizing diverse germplasm for genetic improvement.
基金Supported by the National High Technology Research and Development Program of China (2009AA05Z443)
文摘The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol,an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae.In this study,we investigated the use of salting-out extraction(SOE) that employed a K2HPO4/ethanol system consisting of 21% ethanol and 17% K2HPO4(mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE,about 98% of solid matters was removed,and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%,respectively,and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.
基金the National Natural Science Foundation of China(No.30600086)
文摘Irrigation with various dilutions of seawater can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture in the coastal semi-arid areas of the North China Plain. Jerusalem artichoke (Helianthus tuberosus L.) grown in a field experiment was irrigated with seawater diluted with freshwater from 2001 to 2003 to determine the feasibility of seawater irrigation in the Laizhou area. For treatments of CK (non-irrigation) along with seawater concentrations of 25%, 50%, and 75%, total dissolved solid (TDS) in the non-irrigated soil significantly increased (P ≤ 0.05) in both 2002 and 2003 and was 1.3 times higher in 2003 than in 2001. In the 25% and 50% seawater concentration treatments, TDS in 2001 was significantly greater (P ≤ 0.05) than CK; however, TDS in these two treatments decreased by 34.9% and 40.1%, respectively, in 2003 compared with 2001. The sodium adsorption ratio (SAR) remained below 10 mmol^1/2 L^-1/2, indicating that alkalization was low with seawater irrigation. In 2001 and 2002, compared to CK and the irrigation treatment with 75% seawater, irrigation with 25% and 50% seawater increased the yields of Jerusalem .artichoke. This meant that Jerusalem artichoke could be safely grown in salt-affected land of Laizhou area with 25% and 50% seawater irrigation.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA021205)the National Key Technology R&D Program of China(No.2011BAC02B04)
文摘Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the effects of processing variables (extraction temperature, pH, extraction time, and liquid-to- solid ratio) on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions: pHl .52, 63.62 min, 100℃ and a liquid-to-solid ratio of 44.4 mL/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52+0.90% was obtained, which validated the model prediction. The effects of different drying methods (freeze drying, spray drying and vacuum drying) on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural differences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by different methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin (SDP) and apple pectin (AP) were stronger than those of vacuum oven dried pectin (ODP) and vacuum freeze dried pectin (FDP). Therefore compared with the other two drying methods, the spray drying method was the best.
基金Project supported by the National Natural Science Foundation of China (No. 30470331)
文摘An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.
基金Supported by Xining Comprehensive Test Station Project of National Staple Vegetable Industry Technology System(CARS-23-G-23)
文摘Two varieties of Jerusalem artichoke (early maturing variety Qingyu No.1 and middle maturing variety Qingyu No.2),which were bred independently by Research and Development Center of Jerusalem Artichoke,Qinghai Academy of Agriculture and Forestry,were used as materials,and the content and dynamic changes of three kinds of carbohydrates (sucrose,glucose and fructose) in various organs of Jerusalem artichoke in different periods in two natural habitats (water plain and low hill dry land) were studied.The results showed that the content of the three kinds of carbohydrates in Qingyu No.1 and Qingyu No.2 in the water plain and low hill dry land was relatively high in late June and late August.The total content of the three carbohydrates in Qingyu No.1 peaked earlier than that of Qingyu No.2.The total content of the three carbohydrates in the water plain peaked earlier than that of the low hill dry land.In the whole growth period,the total content of the three carbohydrates in the stems and roots of Jerusalem artichoke was higher than that of the leaves.In the leaves,fructose accumulation was relatively obvious in the whole growth period,and the content was high,followed by glucose.In the stems and roots,fructose content was high,followed by sucrose.In the tubers,glucose accumulation was obvious,and sucrose content was lower than glucose and fructose content.When the tubers were harvested,fructose content was high.
文摘Inulin, an oligosaccharide produced by several plants, has been shown to enhance the viability of probiotic cultures in milk through storage. Jerusalem artichoke ( Helianthus tuberosus L.) is an interested prebiotic because its tuber has risen content of inulin and fructo-oligosaccharides. This study was aimed to: 1) set the effect of Jerusalem artichoke in deferent concentrations (2.5% & 5%) on the growth of probiotic Lb. acidophilus P106 in the bio-yoghurt during cold storage at 5℃ and sensory evaluation of probiotic yoghurts;2) study the effect of feeding with this synbiotic fermented milk on diabetic mice. It could be concluded that the Jerusalem artichoke influenced the growth of Lb. acidophilus P106 and 5% (w/v) Jerusalem artichoke was given the highest growth and sensory evaluation. On the other hand, no serious adverse effects were observed;the reduction of blood glucose was observed at the termination of empirical phase, also, high level (5%) of Jerusalem artichoke led to more reduction of blood glucose, cholesterol levels and total lipids compared with control.
文摘Biochemical characteristics, antimicrobial and quorum quenching activity of the extract of the “Carciofo di Montoro”, a typical ecotype of Cynara cardunculus var. scolymus of the Campania region (Southern Italy) were studied, to consider it as potential reserve of bioactive constituents useful for food industry and beneficial for managing and preventing several chronic illnesses in humans. The extract exhibited a good polyphenol content (528 μg GAE/g) and antioxidant activity (EC50 less than 5 mg). Ultra pressure liquid chromatography (UPLC) revealed high amount of chlorogenic acid, cynarin and epicatechin. The extract showed antimicrobial activity against Escherichia coli, Staphylocccus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus pathogen strains. Finally, quorum quenching activity was demonstrated. The variety Carciofo di Montoro could represent a good source of health-promoting polyphenols, encouraging a nutraceutical use of such ecotype, for several phyto-pharmaceutical applications.
文摘The dynamics of biomass accumulation during the growing period, the yield of leafy stalks and tubers, and the nutrient concentration and nutrient uptake of the yield were investigated for two Jerusalem artichoke varieties (Tápiói Korai and Tápiói Sima) in a field experiment involving mineral fertilisation. Considerable differences were observed between the dynamics of leafy stalk and tuber development in Tápiói Korai which has a short vegetation period and Tápiói Sima where the vegetation period is long. The maximum dry matter ratio between the tuber yield and the leafy stalk yield was 1:1 for Tápiói Korai and 1:4.5 for Tápiói Sima. During the period when the maximum aboveground biomass developed in Tápiói Korai, 100 kg.ha-1 N and P fertiliser resulted in the highest leafy stalk yield (38.34 t.ha-1), while for Tápiói Sima, which developed a much greater leafy stalk mass, the highest aboveground biomass yield (78-80 t.ha-1) was given in response to 200 kg.ha-1 N supplemented by P and K fertiliser. Both artichoke varieties produced the great-est tuber yield at a N rate of 200 kg.ha-1, supplemented with P and K fertiliser. The nutrient concentration in the leafy stalks was highest on the 85th day of the vegetation period, prior to intensive dry matter accumulation in the leafy stalks and before tuber formation began. In both varieties the maximum nutrient uptake was recorded on the 155th day. Great differences were observed between the varieties in terms of specific nutrient uptake. For a tuber yield of 10 t, together with the corresponding leafy stalk yield, the specific nutrient uptake of the Tápiói Korai variety amounted to 48 kg N, 10 kg P, 83 kg K, 30 kg Ca and 10 kg Mg, while for Tápiói Sima these figures were 162 kg N, 30 kg P, 300 kg K, 84 kg Ca and 45 kg Mg.
文摘Fructo-oligosaccharide (FOS) is a type of dietary fiber. It can not be digested by enzymes in the body. It also helps to improve a digestion in the intestine, an excretion system and prevent the cancer. Jerusalem artichoke is scanned and analyzed by High Performance Liquid Chromatography (HPLC) for quantity of FOS. They are selected for extraction and drying process. Studies on the extraction for a powder making, the conditions are optimum for maximum yield. Experiments manage using a factorial real 2 × 4 × 6 in 2 blocks of Randomized Complete Block Design (RCBD) models. The first factor studies on two types of solvent. The second factor is the four levels of temperature in the extraction with water at 25 ℃, 35 ℃, 50℃ and 60℃ for 30 min. The third factor is the ratio of sample per solvent as 5, 6, 10, 15, 20 and 30 times. The data and comparison of average is analyzed by Duncan's New Mutiple Range Test at the significant level 0.05. The concentrated extracts are processed to powder by freeze drying, a hot air and vacuum drying. Optimal conditions control the temperature and time by heating in both vacuum and conventional oven. The results of temperature on the extraction are significantly different at a = 0.05. In drying process with high temperature, the color of product is more yellow (b) and less white (L). The difference of color value is statistically significant at level a = 0.05. The sensory evaluation of food products are added the extracted powder and attribute by panelist. High level acceptance of product is aspectual about texture and flavor. FOS is the trend of functional foods for health. It is focus on low energy and on obesity including increasing the absorption of calcium.
文摘Strains K.marxianus Y-303,Oenoferm credo,Oenoferm rouge,Sacch.vini Rkaseteli-6,Sacch.cerevisiae XII and Sacch.uvarum 73 were used for fermentation of juice,extracted from Jerusalem artichoke tubers grown in Uzbekistan.The fermentation process was carried out in laboratory conditions.Among these yeasts Oenoferm credo,Oenoferm rouge and Sacch.uvarum 73 showed higher performance than K.marxianus Y-303 and the ethanol yields were 92%,90%and 88%,respectively.After 72 h fermentation with Sacch.vini Rkaseteli-6 and Sacch.cerevisiae XII the yield of ethanol was 82%and 74%of maximum theoretical yield,respectively.
文摘Jerusalem artichoke has been cultivated in various regions without any special breeding technique. As a food, Jerusalem artichoke has a characteristic flavour and functional ingredients including inulin, other dietary fibers, minerals and traces of polyphenol. The production of chips from Jerusalem artichoke was examined in this study because it contains high amount of inulin. Inulin gives health promoting effects especially for the people suffering from diabetes. Firstly,chips production from Jerusalem artichoke was studied in the scope of properties like moisture, oil, color, texture, sensory. After cleaning, tubers prepared in specified size and slices were cooked in the deep fat fryer or microwave oven. The best results obtained for frying and microwave oven application of Jerusalem artichoke were obtained at 180℃ for 240s treatment time and at 600 W for105s, respectively.