The challenges and opportunities for developing sustainable plant factories with artificial lighting(PFALs)are discussed.After examining the production cost and productivity of existing PFALs in Japan,the possibility ...The challenges and opportunities for developing sustainable plant factories with artificial lighting(PFALs)are discussed.After examining the production cost and productivity of existing PFALs in Japan,the possibility of introducing a relatively new concept and methodology for considerably improving productivity are discussed in relation to environmental controllability and resource use efficiencies.The fundamental and potential characteristics of ideal or next-generation PFALs(n-PFALs)are then discussed with some suggestions for actualizing n-PFALs.Finally,perspectives of the n-PFALs and technologies to be integrated into the n-PFALs are presented in relation to the Sustainable Development Goals(SDGs)to be achieved by 2030.展开更多
The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was l...The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable,In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.展开更多
文摘The challenges and opportunities for developing sustainable plant factories with artificial lighting(PFALs)are discussed.After examining the production cost and productivity of existing PFALs in Japan,the possibility of introducing a relatively new concept and methodology for considerably improving productivity are discussed in relation to environmental controllability and resource use efficiencies.The fundamental and potential characteristics of ideal or next-generation PFALs(n-PFALs)are then discussed with some suggestions for actualizing n-PFALs.Finally,perspectives of the n-PFALs and technologies to be integrated into the n-PFALs are presented in relation to the Sustainable Development Goals(SDGs)to be achieved by 2030.
基金supported by the Ministry of Education of China and Agriculture and Agri-Food Canada(MOE-AAFC) PhD student research program
文摘The utility of artificial microRNAs（amiRNAs） to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable,In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.
