In this paper we made a thorough and system- atic expatiation of the definition,connotation and composition of bio-manufacture.Based on the dif- ferent development stages and process of bioengi- neering,its main resea...In this paper we made a thorough and system- atic expatiation of the definition,connotation and composition of bio-manufacture.Based on the dif- ferent development stages and process of bioengi- neering,its main research direction and correspond- ing researches were reviewed.Furthermore,from the viewpoint of disciplinary crossing,integration and development,the main technological develop- ment direction of bio-engineering in the coming years was pointed out.展开更多
Metabolic engineering of heterologous resver- atrol production in Saccharomyces cerevisiae faces challenges as the precursor L-tyrosine is stringently regulated by a complex biosynthetic system. We over- expressed the...Metabolic engineering of heterologous resver- atrol production in Saccharomyces cerevisiae faces challenges as the precursor L-tyrosine is stringently regulated by a complex biosynthetic system. We over- expressed the main gene targets in the upstream pathways to investigate their influences on the downstream resver- atrol production. Single-gene overexpression and DNA assembly-directed multigene overexpression affect the production of resveratrol as well as its precursor p-coumaric acid. Finally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.144-3.74 mg.L-1, 2.27 times higher than the initial production in YPD medium （4% glucose）. The newly discovered gene targets TRP1 expressing phosphoribosy- lanthranilate isomerase, AR03 expressing 3-deoxy-D- arabino-heptulosonate-7-phosphate synthase, and 4CL expressing 4-coumaryl-CoA ligase show notable positive impacts on resveratrol production in S. cerevisiae.展开更多
文摘In this paper we made a thorough and system- atic expatiation of the definition,connotation and composition of bio-manufacture.Based on the dif- ferent development stages and process of bioengi- neering,its main research direction and correspond- ing researches were reviewed.Furthermore,from the viewpoint of disciplinary crossing,integration and development,the main technological develop- ment direction of bio-engineering in the coming years was pointed out.
基金The authors declare no competing financial interest. This work was funded by the National Basic Research Program of China （973 Program, Grant No. 2014CB745100） and the National High Technology Research and Development Program of China （863 Program, Grant No. 2012AA02AT01）, the International S＆T Cooperation Program of China （2015DFA00960）, and the National Natural Science Foundation of China （Major Program, Grant No. 21390203）.
文摘Metabolic engineering of heterologous resver- atrol production in Saccharomyces cerevisiae faces challenges as the precursor L-tyrosine is stringently regulated by a complex biosynthetic system. We over- expressed the main gene targets in the upstream pathways to investigate their influences on the downstream resver- atrol production. Single-gene overexpression and DNA assembly-directed multigene overexpression affect the production of resveratrol as well as its precursor p-coumaric acid. Finally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.144-3.74 mg.L-1, 2.27 times higher than the initial production in YPD medium （4% glucose）. The newly discovered gene targets TRP1 expressing phosphoribosy- lanthranilate isomerase, AR03 expressing 3-deoxy-D- arabino-heptulosonate-7-phosphate synthase, and 4CL expressing 4-coumaryl-CoA ligase show notable positive impacts on resveratrol production in S. cerevisiae.
