Monte Carlo simulation was applied to Assembly Success Bate (ASK) analyses. ASR of two peg-in-hole robot assemblies was used as an example by taking component parts' sizes, manufacturing tolerances and robot repea...Monte Carlo simulation was applied to Assembly Success Bate (ASK) analyses. ASR of two peg-in-hole robot assemblies was used as an example by taking component parts' sizes, manufacturing tolerances and robot repeatability into account. A statistic arithmetic expression was proposed and deduced in this paper, which offers an alternative method of estimating the accuracy of ASR, without having to repeat the simulations. This statistic method also helps to choose a suitable sample size, if error reduction is desired. Monte Carlo simulation results demonstrated the feasibility of the method.展开更多
Scalable fabrication of high-rate micro-supercapacitors(MSCs)is highly desired for on-chip integration of energy storage components.By virtue of the special self-assembly behavior of 2D materials during drying thin fi...Scalable fabrication of high-rate micro-supercapacitors(MSCs)is highly desired for on-chip integration of energy storage components.By virtue of the special self-assembly behavior of 2D materials during drying thin films of their liquid dispersion,a new inkjet printing technique of passivated graphene micro-flakes is developed to directly print MSCs with 3D networked porous microstructure.The presence of macroscale through-thickness pores provides fast ion transport pathways and improves the rate capability of the devices even with solid-state electrolytes.During multiple-pass printing,the porous microstructure effectively absorbs the successively printed inks,allowing full printing of 3D structured MSCs comprising multiple vertically stacked cycles of current collectors,electrodes,and sold-state electrolytes.The all-solid-state heterogeneous 3D MSCs exhibit excellent vertical scalability and high areal energy density and power density,evidently outperforming the MSCs fabricated through general printing techniques.展开更多
Objective Lidamycin (LDM) can be dissociated to an apoprotein (LDP) and an active enediyne chromophore (AE). The detached AE can reassemble with its LDP-containing fusion protein to endow the latter with potent ...Objective Lidamycin (LDM) can be dissociated to an apoprotein (LDP) and an active enediyne chromophore (AE). The detached AE can reassemble with its LDP-containing fusion protein to endow the latter with potent antitumor activity. However, the reassembly of AE with LDP is affected by several factors. Our aim was to optimize the assembly efficiency of the AE with a LDP-containing fusion protein and investigate the influence of several factors on the assembly efficacy. Methods A method based on RP-HPLC was developed to analyze the assembly rate, and an orthogonal experimental design L9 (3^4) was used to investigate the effects of temperature, assembly time, pH and molecular ratio of LDP-containing fusion protein to AE on the assembly rate. Furthermore, the determined optimum conditions for the assembly rate of the LDP-containing fusion protein with AE were applied and evaluated. Results A calibration curve based on the LDM micromolar concentration against the peak-area of AE by HPLC was obtained. The order in which individual factors in the orthogonal experiment affected the assembly rate were temperature〉time〉pH〉molar ratio of AE to protein and all were statistically significant (P〈0.01). The optimal assembly conditions were temperature at 10 ℃, time of 12 h, pH 7.0, and the molar ratio of AE: protein of 5:1. The assembly rate of AE with a LDP-containing fusion protein was improved by 23% after condition optimization. Conclusion The assembly rate of chromophore of lidamycin with its LDP-containing fusion protein was improved after condition optimization by orthogonal design, and the optimal conditions described herein should prove useful for the development of this type of LDP-containing fusion protein.展开更多
To validate neutronics calculation for the blanket design of fusion-fission hybrid reactor,experiments for measuring reaction rates inside two simulating assemblies are performed.Two benchmark assemblies were develope...To validate neutronics calculation for the blanket design of fusion-fission hybrid reactor,experiments for measuring reaction rates inside two simulating assemblies are performed.Two benchmark assemblies were developed for the neutronics experiments.A D-T fusion neutron source is placed at the center of the setup.One of them consists of three layers of depleted uranium shells and two layers of polyethylene shells,and these shells are arranged alternatively.The ^(238)U capture reaction rates are measured using depleted uranium foils and an HPGe gamma spectrometer.The fission reaction rates are measured using a fission chamber coated with depleted uranium.The other assembly consists of depleted uranium and LiH shells.The tritium production rates are measured using the lithium glass scintillation detector which is placed in the LiH region of the assembly.The measured reaction rates are compared with the calculated ones predicted using MCNP code,and C/E values are obtained.展开更多
Most existing force feedback methods are still difficult to meet the requirements of real-time force calculation in virtual assembly and operation with complex objects. In addition, there is often an assumption that t...Most existing force feedback methods are still difficult to meet the requirements of real-time force calculation in virtual assembly and operation with complex objects. In addition, there is often an assumption that the controlled objects are completely flee and the target object is only completely fixed or flee, thus, the dynamics of the kinematic chain where the controlled objects are located are neglected during the physical simulation of the product manipulation with force feedback interaction. This paper proposes a physical simulation method of product assembly and operation manipulation based on statistically learned contact force prediction model and the coupling of force feedback and dynamics. In the proposed method, based on hidden Markov model (HMM) and local weighting learning (LWL), contact force prediction model is constructed, which can estimate the contact force in real time during interaction. Based on computational load balance model, the computing resources are dynamically assigned and the dynamics integral step is optimized. In addition, smoothing process is performed to the force feedback on the synchronization points. Consequently, we can solve the coupling and synchronization problems of high-frequency feedback force servo. low-frequency dynamics solver servo and scene rendering servo, and realize highly stable and accurate force feedback in the physical simulation of product assembly and operation manipulation. This research proposes a physical simulation method of product assembly and operation manipulation.展开更多
The qualitative and quantitative assessment of gas flow has become increasingly relevant in the use of everyday systems. The micro flow sensor, developed by Innovative Sensor Technology AG (Switzerland), is by princ...The qualitative and quantitative assessment of gas flow has become increasingly relevant in the use of everyday systems. The micro flow sensor, developed by Innovative Sensor Technology AG (Switzerland), is by principle a calorimetric flow sensor produced as a micro system on a glass substrate by means of photolithography and glass etching technology. These structures are arranged as a platinum micro heater and sensor in a Wheatstone bridge. The subsequent etching process produces an exposed area of polyimide membrane that is only a few microns thick and includes the resistive sensor structure as the active area. In addition, the RTD (resistance temperature detector) technology included on the sensor allows for the implementation of a variety of electronic biasing and signal processing modes. Since the sensor can be powered and the bridge can be measured in both CTA (constant temperature anemometer) and calorimetric mode, new possibilities are presented for both low and high flow rates with regard to temperature compensation, self-calibration and self-monitoring.展开更多
A multi-shot target assembly and automatic alignment procedure for laser–plasma proton acceleration at high repetition rate are introduced.The assembly is based on a multi-target rotating wheel capable of hosting mor...A multi-shot target assembly and automatic alignment procedure for laser–plasma proton acceleration at high repetition rate are introduced.The assembly is based on a multi-target rotating wheel capable of hosting more than 5000 targets,mounted on a 3D motorized stage to allow rapid replenishment and alignment of the target material between laser irradiations.The automatic alignment procedure consists of a detailed mapping of the impact positions at the target surface prior to the irradiation that ensures stable operation of the target,which alongside the purpose-built design of the target wheel,enables operation at rates up to 10 Hz.Stable and continuous laser-driven proton acceleration at 10 Hz is demonstrated,with observed cut-off energy stability about 15%.展开更多
The dynamic balance assessment during the assembly of the coordinator gyroscope significantly impacts the guidance accuracy of precision-guided equipment.In dynamic balance debugging,reliance on rudimentary counterwei...The dynamic balance assessment during the assembly of the coordinator gyroscope significantly impacts the guidance accuracy of precision-guided equipment.In dynamic balance debugging,reliance on rudimentary counterweight empirical formulas persists,resulting in suboptimal debugging accuracy and an increased repetition rate.To mitigate this challenge,we present a multi-head residual graph attention network(ResGAT)model,designed to predict dynamic balance counterweights with high precision.In this research,we employ graph neural networks for interaction feature extraction from assembly graph data.An SDAE-GPC model is designed for the assembly condition classification to derive graph data inputs for the ResGAT regression model,which is capable of predicting gyroscope counterweights under small-sample conditions.The results of our experiments demonstrate the effectiveness of the proposed approach in predicting dynamic gyroscope counterweight in its assembly process.Our approach surpasses current methods in mitigating repetition rates and enhancing the assembly efficiency of gyroscopes.展开更多
Coastal marshes are transitional areas between terrestrial and aquatic ecosystems.They are sensitive to climate change and anthropogenic activities.In recent decades,the reclamation of coastal marshes has greatly incr...Coastal marshes are transitional areas between terrestrial and aquatic ecosystems.They are sensitive to climate change and anthropogenic activities.In recent decades,the reclamation of coastal marshes has greatly increased,and its effects on microbial communities in coastal marshes have been studied with great interest.Most of these studies have explained the short-term spatiotemporal variation in soil microbial community dynamics.However,the impact of reclamation on the community composition and assembly processes of functional microbes(e.g.,ammonia-oxidizing prokaryotes)is often ignored.In this study,using quantitative polymerase chain reaction and the Ion S5™XL sequencing platform,we investigated the spatiotemporal dynamics,assembly processes,and diversity patterns of ammonia-oxidizing prokaryotes in 1000-year-old reclaimed coastal salt marshes.The taxonomic and phylogenetic diversity and composition of ammonia oxidizers showed apparent spatiotemporal variations with soil reclamation.Phylogenetic null modelling-based analysis showed that across all sites,the archaeal ammonia-oxidizing community was assembled by a deterministic process(84.71%),and deterministic processes were also dominant(55.2%)for ammonia-oxidizing bacterial communities except for communities at 60 years of reclamation.The assembly process and nitrification activity in reclaimed soils were positively correlated.The abundance of the amoA gene and changes in ammonia-oxidizing archaeal and bacterial diversities significantly affected the nitrification activity in reclaimed soils.These findings suggest that long-term coastal salt marsh reclamation affects nitrification by modulating the activities of ammonia-oxidizing microorganisms and regulating their community structures and assembly processes.These results provide a better understanding of the effects of long-term land reclamation on soil nitrogen-cycling microbial communities.展开更多
文摘Monte Carlo simulation was applied to Assembly Success Bate (ASK) analyses. ASR of two peg-in-hole robot assemblies was used as an example by taking component parts' sizes, manufacturing tolerances and robot repeatability into account. A statistic arithmetic expression was proposed and deduced in this paper, which offers an alternative method of estimating the accuracy of ASR, without having to repeat the simulations. This statistic method also helps to choose a suitable sample size, if error reduction is desired. Monte Carlo simulation results demonstrated the feasibility of the method.
基金financial support of the Swedish Research Council through the Marie Sklodowska-Curie International Career Grant (No.2015-00395,co-funded by Marie Sklodowska-Curie Actions, through the Project INCA 600398)the Formas Foundation through the Future Research Leaders Grant (No.2016-00496)+3 种基金the AForsk Foundation (Grant No.17-352)the Olle Engkvist Byggmastare Foundation (Grant No.2014/799)the Academy of Finland (Grant No.288945 and 319408)Academy of Finland Research Infrastructure "Printed Intelligence Infrastructure" (PII-FIRI,Grant No. 320019)
文摘Scalable fabrication of high-rate micro-supercapacitors(MSCs)is highly desired for on-chip integration of energy storage components.By virtue of the special self-assembly behavior of 2D materials during drying thin films of their liquid dispersion,a new inkjet printing technique of passivated graphene micro-flakes is developed to directly print MSCs with 3D networked porous microstructure.The presence of macroscale through-thickness pores provides fast ion transport pathways and improves the rate capability of the devices even with solid-state electrolytes.During multiple-pass printing,the porous microstructure effectively absorbs the successively printed inks,allowing full printing of 3D structured MSCs comprising multiple vertically stacked cycles of current collectors,electrodes,and sold-state electrolytes.The all-solid-state heterogeneous 3D MSCs exhibit excellent vertical scalability and high areal energy density and power density,evidently outperforming the MSCs fabricated through general printing techniques.
基金supported by grants from "Significant new drug development" Science and Technology Major Projects of China (2009ZX09301-003 2009ZX09401-005 2010ZX09401-407)
文摘Objective Lidamycin (LDM) can be dissociated to an apoprotein (LDP) and an active enediyne chromophore (AE). The detached AE can reassemble with its LDP-containing fusion protein to endow the latter with potent antitumor activity. However, the reassembly of AE with LDP is affected by several factors. Our aim was to optimize the assembly efficiency of the AE with a LDP-containing fusion protein and investigate the influence of several factors on the assembly efficacy. Methods A method based on RP-HPLC was developed to analyze the assembly rate, and an orthogonal experimental design L9 (3^4) was used to investigate the effects of temperature, assembly time, pH and molecular ratio of LDP-containing fusion protein to AE on the assembly rate. Furthermore, the determined optimum conditions for the assembly rate of the LDP-containing fusion protein with AE were applied and evaluated. Results A calibration curve based on the LDM micromolar concentration against the peak-area of AE by HPLC was obtained. The order in which individual factors in the orthogonal experiment affected the assembly rate were temperature〉time〉pH〉molar ratio of AE to protein and all were statistically significant (P〈0.01). The optimal assembly conditions were temperature at 10 ℃, time of 12 h, pH 7.0, and the molar ratio of AE: protein of 5:1. The assembly rate of AE with a LDP-containing fusion protein was improved by 23% after condition optimization. Conclusion The assembly rate of chromophore of lidamycin with its LDP-containing fusion protein was improved after condition optimization by orthogonal design, and the optimal conditions described herein should prove useful for the development of this type of LDP-containing fusion protein.
基金supported by the National Special Magnetic Confinement Fusion Energy Research (No.2010GB111002),China
文摘To validate neutronics calculation for the blanket design of fusion-fission hybrid reactor,experiments for measuring reaction rates inside two simulating assemblies are performed.Two benchmark assemblies were developed for the neutronics experiments.A D-T fusion neutron source is placed at the center of the setup.One of them consists of three layers of depleted uranium shells and two layers of polyethylene shells,and these shells are arranged alternatively.The ^(238)U capture reaction rates are measured using depleted uranium foils and an HPGe gamma spectrometer.The fission reaction rates are measured using a fission chamber coated with depleted uranium.The other assembly consists of depleted uranium and LiH shells.The tritium production rates are measured using the lithium glass scintillation detector which is placed in the LiH region of the assembly.The measured reaction rates are compared with the calculated ones predicted using MCNP code,and C/E values are obtained.
基金Supported by National Natural Science Foundation of China(51475418)National Basic Research 973 Program of China(2011CB706503)Science Fund for Creative Research Groups of National Natural Science Foundation of China(51221004)
文摘Most existing force feedback methods are still difficult to meet the requirements of real-time force calculation in virtual assembly and operation with complex objects. In addition, there is often an assumption that the controlled objects are completely flee and the target object is only completely fixed or flee, thus, the dynamics of the kinematic chain where the controlled objects are located are neglected during the physical simulation of the product manipulation with force feedback interaction. This paper proposes a physical simulation method of product assembly and operation manipulation based on statistically learned contact force prediction model and the coupling of force feedback and dynamics. In the proposed method, based on hidden Markov model (HMM) and local weighting learning (LWL), contact force prediction model is constructed, which can estimate the contact force in real time during interaction. Based on computational load balance model, the computing resources are dynamically assigned and the dynamics integral step is optimized. In addition, smoothing process is performed to the force feedback on the synchronization points. Consequently, we can solve the coupling and synchronization problems of high-frequency feedback force servo. low-frequency dynamics solver servo and scene rendering servo, and realize highly stable and accurate force feedback in the physical simulation of product assembly and operation manipulation. This research proposes a physical simulation method of product assembly and operation manipulation.
文摘The qualitative and quantitative assessment of gas flow has become increasingly relevant in the use of everyday systems. The micro flow sensor, developed by Innovative Sensor Technology AG (Switzerland), is by principle a calorimetric flow sensor produced as a micro system on a glass substrate by means of photolithography and glass etching technology. These structures are arranged as a platinum micro heater and sensor in a Wheatstone bridge. The subsequent etching process produces an exposed area of polyimide membrane that is only a few microns thick and includes the resistive sensor structure as the active area. In addition, the RTD (resistance temperature detector) technology included on the sensor allows for the implementation of a variety of electronic biasing and signal processing modes. Since the sensor can be powered and the bridge can be measured in both CTA (constant temperature anemometer) and calorimetric mode, new possibilities are presented for both low and high flow rates with regard to temperature compensation, self-calibration and self-monitoring.
基金This work was supportedby the Spanish Ministerio de Ciencia,Innovacion y Universidades under grants RTI2018-101578-B-C21,RTI2018-101578-B-C22,FPI predoctorals BES-2017-08917 and PRE2019-090730Unidad de Excelencia Maria de Maetzu under project MdM-2016-0692-17-2+2 种基金the Xuntade Galicia grants GRC ED431C 2017/54 and ED431F2023/21 and a grant of the program Grupos de investigacion consolidados(CIAICO/2022/008)financed by Generalitat Valenciana.Action co-financed by the European Union through the Programa Operativo del Fondo Europeo de Desarrollo Regional(FEDER)of the Comunitat Valenciana 2014-2020(IDIFEDER/2021/002)This work was supported by‘la Caixa'Foundation(ID 100010434)(fellowship code LCF/BQ/PI20/11760027)grant RYC2021-032654-I funded by MICIU/AEI/10.13039/501100011033 and by‘European Union Next Generation EU'.
文摘A multi-shot target assembly and automatic alignment procedure for laser–plasma proton acceleration at high repetition rate are introduced.The assembly is based on a multi-target rotating wheel capable of hosting more than 5000 targets,mounted on a 3D motorized stage to allow rapid replenishment and alignment of the target material between laser irradiations.The automatic alignment procedure consists of a detailed mapping of the impact positions at the target surface prior to the irradiation that ensures stable operation of the target,which alongside the purpose-built design of the target wheel,enables operation at rates up to 10 Hz.Stable and continuous laser-driven proton acceleration at 10 Hz is demonstrated,with observed cut-off energy stability about 15%.
基金supported by the NationalNatural Science Foundation of China(No.51705100)the Foundation of Research on Intelligent Design Method Based on Knowledge Space Reconstruction and Perceptual Push(No.52075120).
文摘The dynamic balance assessment during the assembly of the coordinator gyroscope significantly impacts the guidance accuracy of precision-guided equipment.In dynamic balance debugging,reliance on rudimentary counterweight empirical formulas persists,resulting in suboptimal debugging accuracy and an increased repetition rate.To mitigate this challenge,we present a multi-head residual graph attention network(ResGAT)model,designed to predict dynamic balance counterweights with high precision.In this research,we employ graph neural networks for interaction feature extraction from assembly graph data.An SDAE-GPC model is designed for the assembly condition classification to derive graph data inputs for the ResGAT regression model,which is capable of predicting gyroscope counterweights under small-sample conditions.The results of our experiments demonstrate the effectiveness of the proposed approach in predicting dynamic gyroscope counterweight in its assembly process.Our approach surpasses current methods in mitigating repetition rates and enhancing the assembly efficiency of gyroscopes.
基金financially supported by the Ningbo Science and Technology Bureau,China (Nos.2021S018 and 2022Z169)the National Natural Science Foundation of China (No.42077026)
文摘Coastal marshes are transitional areas between terrestrial and aquatic ecosystems.They are sensitive to climate change and anthropogenic activities.In recent decades,the reclamation of coastal marshes has greatly increased,and its effects on microbial communities in coastal marshes have been studied with great interest.Most of these studies have explained the short-term spatiotemporal variation in soil microbial community dynamics.However,the impact of reclamation on the community composition and assembly processes of functional microbes(e.g.,ammonia-oxidizing prokaryotes)is often ignored.In this study,using quantitative polymerase chain reaction and the Ion S5™XL sequencing platform,we investigated the spatiotemporal dynamics,assembly processes,and diversity patterns of ammonia-oxidizing prokaryotes in 1000-year-old reclaimed coastal salt marshes.The taxonomic and phylogenetic diversity and composition of ammonia oxidizers showed apparent spatiotemporal variations with soil reclamation.Phylogenetic null modelling-based analysis showed that across all sites,the archaeal ammonia-oxidizing community was assembled by a deterministic process(84.71%),and deterministic processes were also dominant(55.2%)for ammonia-oxidizing bacterial communities except for communities at 60 years of reclamation.The assembly process and nitrification activity in reclaimed soils were positively correlated.The abundance of the amoA gene and changes in ammonia-oxidizing archaeal and bacterial diversities significantly affected the nitrification activity in reclaimed soils.These findings suggest that long-term coastal salt marsh reclamation affects nitrification by modulating the activities of ammonia-oxidizing microorganisms and regulating their community structures and assembly processes.These results provide a better understanding of the effects of long-term land reclamation on soil nitrogen-cycling microbial communities.