期刊文献+
共找到132,339篇文章
< 1 2 250 >
每页显示 20 50 100
易黄汤对脾虚湿热型宫颈癌小鼠皮下移植瘤cGAS/STING/IRF-3信号通路及相关免疫因子的影响
1
作者 林兰 吴荣莉 +1 位作者 郑怡真 吴冬梅 《福建中医药》 2024年第4期10-14,共5页
目的探讨易黄汤对脾虚湿热型宫颈癌小鼠皮下移植瘤环磷酸鸟苷-腺苷酸合成酶(cGAS)/干扰素刺激基因(STING)/干扰素调节因子-3(IRF-3)信号通路及相关免疫因子的影响。方法选取6~8周龄SPF级雌性BALB/c小鼠50只,随机分为造模组40只和空白组1... 目的探讨易黄汤对脾虚湿热型宫颈癌小鼠皮下移植瘤环磷酸鸟苷-腺苷酸合成酶(cGAS)/干扰素刺激基因(STING)/干扰素调节因子-3(IRF-3)信号通路及相关免疫因子的影响。方法选取6~8周龄SPF级雌性BALB/c小鼠50只,随机分为造模组40只和空白组10只。空白组正常喂养,造模组通过湿热环境及高脂高糖饮食建立脾虚湿热型小鼠模型,当小鼠出现嗜卧懒动、易激惹、形体消瘦、进食及饮水量减少、毛发疏松粗糙、粪便时干时溏、肛温稍有增加则认为造模成功。将2×106个/mL对数生长期HPV16阳性人宫颈癌CaSki细胞悬液0.2 mL接种于上述50只小鼠右腋下,5~7 d后可触及直径≥5 mm结节时即认为皮下移植瘤造模成功。将造模组按随机数字表法分为模型组和易黄汤低剂量、中剂量、高剂量组,每组10只。低、中、高剂量组均按体质量40.0 mL/kg分别予含生药浓度0.25、0.50、1.00 g/mL易黄汤药液灌胃,模型组和空白组予同等剂量生理盐水灌胃,均隔日1次,干预4周。干预后计算5组小鼠胸腺、脾脏指数和瘤质量、抑瘤率;ELISA法检测5组小鼠血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;支链DNA信号扩增法检测5组小鼠皮下移植瘤组织HPV E6 mRNA转录水平;Western blot测定5组小鼠移植瘤组织中cGAS、STING、IRF-3蛋白表达量。结果与空白组比较,模型组胸腺和脾脏指数、IL-6、TNF-α水平显著下降(P<0.05),HPV E6 mRNA转录水平显著增加(P<0.05);易黄汤不同剂量组中以高剂量组的抑瘤率最高为(35.42±4.86)%(P<0.05);与模型组比较,低、中、高剂量组胸腺和脾脏指数、IL-6、TNF-α水平及cGAS、STING和IRF-3蛋白表达量显著增加(P<0.05),皮下移植瘤质量及HPV E6 mRNA转录水平显著降低(P<0.05),且降低程度与易黄汤剂量成正比。结论脾虚湿热能促进CaSki细胞生长、HPV表达增加,易黄汤可能通过激活cGAS/STING/IRF-3信号通路增强免疫功能,从而抑制HPV表达及宫颈癌小鼠移植瘤生长。 展开更多
关键词 宫颈癌 易黄汤 移植瘤 cgaS/STING/IRF-3信号通路 免疫因子
下载PDF
CGA护理模式在老年冠心病患者PCI术后中的应用效果研究
2
作者 李立莉 厉敏 卞秀敏 《中文科技期刊数据库(文摘版)医药卫生》 2024年第2期164-168,共5页
研究CGA护理模式对老年冠心病心绞痛患者PCI术后应对方式及生活质量的影响。方法 选择2021年1月-2022年7月我院收治冠心病心绞痛患者100例,掷硬币法分两组。对照组标准程序化护理,观察组CGA护理。结果 干预后观察组MCMQ回避和屈服维度、... 研究CGA护理模式对老年冠心病心绞痛患者PCI术后应对方式及生活质量的影响。方法 选择2021年1月-2022年7月我院收治冠心病心绞痛患者100例,掷硬币法分两组。对照组标准程序化护理,观察组CGA护理。结果 干预后观察组MCMQ回避和屈服维度、LVEDD、不良事件低于对照组,P<0.05。MCMQ面对维度、6MWT、LVEF、生活质量和疾病管理能力高于对照组,P<0.05。结论 CGA护理模式可以有效地增强老年冠心病心绞痛患者的应对方式。 展开更多
关键词 cga 老年综合评估 冠心病 心绞痛 应对方式 生活质量
下载PDF
老年冠心病病人应用基于CGA的专科干预与反馈式健康宣教的效果观察
3
作者 史小华 戴海蓉 +1 位作者 王颖 顾晓燕 《循证护理》 2024年第7期1233-1237,共5页
目的:探讨基于老年综合评估(CGA)的专科干预结合反馈式健康宣教在老年冠心病病人中的应用效果。方法:选取2020年10月—2022年10月本院收治的100例老年冠心病病人,采用随机数字表法分为甲组和乙组,各50例。甲组采用常规老年护理及健康教... 目的:探讨基于老年综合评估(CGA)的专科干预结合反馈式健康宣教在老年冠心病病人中的应用效果。方法:选取2020年10月—2022年10月本院收治的100例老年冠心病病人,采用随机数字表法分为甲组和乙组,各50例。甲组采用常规老年护理及健康教育,乙组予以基于CGA的专科干预联合反馈式健康宣教。观察两组应对方式、健康教育需求、自我管理能力及心脏不良事件发生情况。结果:与干预前相比,两组随访6个月的医学应对方式问卷(MCMQ)面对维度评分均升高,回避、屈服维度评分均降低,且乙组面对维度评分高于甲组,回避、屈服维度评分低于甲组,差异有统计学意义(P<0.05);两组随访6个月的心脏康复信息需求表(INCR)评分均低于干预前,冠心病自我管理量表(CSMS)评分均高于干预前,且乙组INCR评分较甲组低,CSMS评分较甲组高,差异有统计学意义(P<0.05);乙组随访6个月内心脏不良事件发生率低于甲组,差异有统计学意义(P<0.05)。结论:CGA下专科干预联合反馈式健康宣教能够改善老年冠心病病人对应对方式,满足其心脏康复健康教育需求,有助于提高病人自我管理能力,改善心脏不良事件控制效果。 展开更多
关键词 老年综合评估(cga) 健康教育 冠心病 应对方式 不良事件
下载PDF
cGAS-STING信号通路调节剂在免疫治疗中的研究进展
4
作者 娄方宁 郑明月 +1 位作者 陈凯先 张素林 《中国药科大学学报》 CAS CSCD 北大核心 2024年第1期15-25,共11页
环鸟嘌呤-腺嘌呤核苷酸合成酶(cGAS)-干扰素基因刺激蛋白(STING)信号通路感知细胞质中的异常双链DNA后,诱导Ⅰ型干扰素(IFN-Ⅰ)和促炎细胞因子表达,从而激活宿主的免疫应答,增强机体抗肿瘤免疫反应和抗病原体感染。但是,cGAS-STING信号... 环鸟嘌呤-腺嘌呤核苷酸合成酶(cGAS)-干扰素基因刺激蛋白(STING)信号通路感知细胞质中的异常双链DNA后,诱导Ⅰ型干扰素(IFN-Ⅰ)和促炎细胞因子表达,从而激活宿主的免疫应答,增强机体抗肿瘤免疫反应和抗病原体感染。但是,cGAS-STING信号通路的持续激活会驱动自身免疫性疾病、衰老相关炎症和神经退行性病变等疾病。本文阐述了cGAS-STING信号通路参与调控多种免疫相关性疾病发生发展的机制,重点回顾了STING激动剂、cGAS抑制剂以及STING抑制剂的研发进展,为cGAS-STING调节剂的研发提供更多理论参考。 展开更多
关键词 cgaS-STING信号通路 STING激动剂 cgaS抑制剂 STING抑制剂 免疫治疗
下载PDF
高良姜素通过激活cGAS/STING信号通路抑制骨肉瘤MG63细胞的恶性生物学行为
5
作者 加鹏飞 余小超 +1 位作者 刘小波 李鹏程 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2024年第7期675-680,共6页
目的:探讨高良姜素(Gal)是否通过调节cGAS/STING信号通路影响骨肉瘤MG63细胞增殖、迁移、侵袭和凋亡。方法:体外培养人骨肉瘤MG63细胞,分别使用0、5、15、25、50、100、200μmol/L的Gal培养48 h后,CCK-8法检测Gal对细胞活力的影响。将M... 目的:探讨高良姜素(Gal)是否通过调节cGAS/STING信号通路影响骨肉瘤MG63细胞增殖、迁移、侵袭和凋亡。方法:体外培养人骨肉瘤MG63细胞,分别使用0、5、15、25、50、100、200μmol/L的Gal培养48 h后,CCK-8法检测Gal对细胞活力的影响。将MG63细胞分为对照组(未处理细胞)、Gal低浓度组(Gal-L组,50μmol/L Gal处理)、Gal高浓度组(Gal-H组,100μmol/L Gal处理)和Gal-H+STING抑制剂组(Gal-H+H-151组,100μmol/L Gal+8μmol/L H-151处理)。采用EdU染色法、划痕愈合实验、Transwell小室法、流式细胞术检测各组细胞增殖活力、迁移、侵袭和凋亡能力,WB法检测各组细胞中cGAS、STING蛋白的表达水平。结果:与对照组比较,Gal-L组、Gal-H组细胞增殖活力、迁移、侵袭能力均显著降低(均P<0.05),细胞凋亡率和cGAS、STING蛋白表达水平均显著升高(均P<0.05);与Gal-H组比较,Gal-H+H-151组细胞增殖活力、迁移和侵袭能力均显著升高(均P<0.05),细胞凋亡率和cGAS、STING蛋白表达水平均显著降低(均P<0.05)。结论:Gal可能通过激活cGAS/STING信号通路抑制骨肉瘤MG63细胞增殖、迁移、侵袭并促进细胞凋亡。 展开更多
关键词 高良姜素 骨肉瘤 MG63细胞 增殖 迁移 侵袭 凋亡 cgaS/STING信号通路
下载PDF
环鸟苷酸-腺苷酸合成酶(cGAS)翻译后修饰在固有免疫中的研究进展
6
作者 韦雅芳 刘纪实 +2 位作者 李怡素 潘诗琪 唐又周 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2024年第2期179-185,共7页
环鸟苷酸-腺苷酸合成酶(cGAS)作为一种核酸转移酶,主要通过感受核酸、激活下游通路并调控Ⅰ型干扰素的合成参与固有免疫反应。cGAS蛋白功能的调节与细菌病毒感染、自身免疫系统疾病及肿瘤等多种疾病相关,而翻译后修饰是目前研究最深入... 环鸟苷酸-腺苷酸合成酶(cGAS)作为一种核酸转移酶,主要通过感受核酸、激活下游通路并调控Ⅰ型干扰素的合成参与固有免疫反应。cGAS蛋白功能的调节与细菌病毒感染、自身免疫系统疾病及肿瘤等多种疾病相关,而翻译后修饰是目前研究最深入和广泛的cGAS蛋白功能调节方式之一。cGAS蛋白翻译后修饰主要有磷酸化、乙酰化、泛素化、小泛素相关修饰(SUMO)化、肽链剪切及谷氨酰化。本文不但系统总结了不同生理和病理条件下cGAS的翻译后修饰如何调控cGAS功能,而且深入挖掘了以cGAS翻译后修饰作为疾病治疗靶点的潜力。 展开更多
关键词 翻译后修饰 环鸟苷酸-腺苷酸合成酶(cgaS) 固有免疫 综述
下载PDF
桂枝芍药知母汤抑制cGAS/STING/NF-κB通路对类风湿关节炎大鼠炎症活动的影响
7
作者 丁明辉 曹茸 +4 位作者 徐鹏刚 任宝娣 李映雪 吕水英 马俊福 《现代中西医结合杂志》 CAS 2024年第8期1053-1059,共7页
目的基于环状GMP-AMP合成酶(cGAS)/干扰素基因刺激因子(STING)/核因子-κB(NF-κB)通路探讨桂枝芍药知母汤对类风湿关节炎大鼠炎症活动的影响及机制。方法取70只Wistar大鼠,随机选择10只作为正常组,其余60只大鼠制备胶原诱导性关节炎模... 目的基于环状GMP-AMP合成酶(cGAS)/干扰素基因刺激因子(STING)/核因子-κB(NF-κB)通路探讨桂枝芍药知母汤对类风湿关节炎大鼠炎症活动的影响及机制。方法取70只Wistar大鼠,随机选择10只作为正常组,其余60只大鼠制备胶原诱导性关节炎模型。将造模成功大鼠随机分为模型组、甲氨蝶呤组、雷公藤组及桂芍知母汤低、中、高剂量组,每组10只。甲氨蝶呤组给予0.104 mg/mL的甲氨蝶呤片混悬液灌胃,1次/周,其余时间每天给予蒸馏水灌胃1次;雷公藤组给予0.625 mg/mL的雷公藤多甙片混悬液灌胃,桂芍知母汤低、中、高剂量组分别给予0.958 g/mL、1.916 g/mL、2.874 g/mL的桂枝芍药知母汤灌胃,正常组、模型组给予蒸馏水灌胃,均1次/d。各组均干预4周。测量各组大鼠干预1,2,3,4周末足趾肿胀程度,并计算关节炎指数(AI);末次干预结束后,ELISA法检测血清白细胞介素-1β(IL-1β)、白细胞介素-17(IL-17)、肿瘤坏死因子-α(TNF-α)水平,Western blot法检测滑膜组织中cGAS、STING、NF-κB p65、IκBα蛋白表达情况。结果干预1,2,3,4周末,模型组大鼠的关节肿胀度均明显高于正常组(P均<0.05);随时间推移,模型组大鼠的关节肿胀度逐渐增加,各药物干预组大鼠的关节肿胀度有不同程度的降低,其中桂芍知母汤中剂量组干预4周末的关节肿胀度明显低于模型组(P<0.05)。干预1周末,各组间AI评分比较差异均无统计学意义(P均>0.05);干预2,3,4周末,桂芍知母汤高剂量组AI评分均明显低于模型组和桂芍知母汤低剂量组(P均<0.05),且桂芍知母汤高剂量组干预2周末的AI评分明显低于雷公藤组(P<0.05);干预3周末,甲氨蝶呤组、桂芍知母汤中剂量组AI评分均明显低于模型组(P均<0.05);干预4周末,甲氨蝶呤组、雷公藤组、桂芍知母汤中剂量组、桂芍知母汤低剂量组AI评分均明显低于模型组(P均<0.05)。与正常组比较,模型组血清IL-1β、IL-17、TNF-α水平及滑膜组织中cGAS、STING、NF-κB p65蛋白相对表达量均明显升高(P均<0.05),滑膜组织中IκBα蛋白相对表达量明显降低(P<0.05);与模型组比较,各药物干预组血清IL-1β、IL-17、TNF-α水平及滑膜组织中cGAS、STING、NF-κB p65蛋白相对表达量均明显降低(P均<0.05),滑膜组织中IκBα蛋白相对表达量均明显升高(P均<0.05)。桂芍知母汤高剂量组和甲氨蝶呤组血清IL-1β、IL-17和TNF-α水平及滑膜组织中cGAS、STING、NF-κB p65蛋白相对表达量均明显低于桂芍知母汤低剂量组(P均<0.05),滑膜组织中IκBα蛋白相对表达量均明显高于桂芍知母汤低剂量组(P均<0.05)。结论桂枝芍药知母汤可明显减轻类风湿关节炎大鼠关节肿胀和关节炎症,机制可能与抑制cGAS/STING/NF-κB通路,降低炎症因子水平有关。 展开更多
关键词 类风湿关节炎 桂枝芍药知母汤 环状GMP-AMP合成酶(cgaS)/干扰素基因刺激因子(STING)/核因子-κB通路 关节炎指数
下载PDF
电针对前交叉韧带断裂大鼠炎症反应及cGAS/STING信号通路的影响
8
作者 贾飞阳 李萌萌 +7 位作者 王金玲 刘静 黄夏荣 孙光华 钟培瑞 彭婷 曾国锋 周君 《中医药导报》 2024年第5期1-6,12,共7页
目的:探究电针对前交叉韧带断裂后创伤性骨关节炎早期炎症反应与环状GMP-AMP合成酶(cGAS)/干扰素基因刺激因子(STING)信号通路的影响。方法:24只SD大鼠随机分为假手术组、前交叉韧带损伤(ACLT)组和电针组,每组8只。其中ACLT组和电针组... 目的:探究电针对前交叉韧带断裂后创伤性骨关节炎早期炎症反应与环状GMP-AMP合成酶(cGAS)/干扰素基因刺激因子(STING)信号通路的影响。方法:24只SD大鼠随机分为假手术组、前交叉韧带损伤(ACLT)组和电针组,每组8只。其中ACLT组和电针组实施前交叉韧带切断术。电针组大鼠予双侧“阳陵泉”“血海”“足三里”“太溪”电针刺激,1次/d,每周干预5 d,连续2周,其余两组不做处理。2周后ELISA法检测大鼠血清白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平;取大鼠左膝关节软骨组织切片分别进行番红O-固绿染色和苏木精-伊红(HE)染色,镜下观察软骨损伤程度并评分(Mankin’s评分);RT-qPCR检测大鼠右膝关节软骨基质金属蛋白酶-13(MMP-13)mRNA、cGAS mRNA、STING mRNA表达水平。Western blotting检测大鼠右膝关节软骨MMP-13、cGAS、STING蛋白表达水平。结果:ACLT组大鼠血清IL-1β、TNF-α水平均高于假手术组(P<0.05);电针组大鼠血清IL-1β、TNF-α水平均低于ACLT组(P<0.05)。番红O-固绿染色显示假手术组大鼠软骨表面光滑;ACLT组大鼠软骨表面出现轻微缺损和细小裂层;电针组大鼠软骨表面较平滑,无明显裂层。HE染色显示假手术组软骨表面平整且光滑,软骨细胞均排列规整;ACLT组大鼠软骨层表面变薄;电针组大鼠软骨表面略粗糙。ACLT组大鼠Mankin’s评分高于假手术组(P<0.05);电针组大鼠Mankin’s评分低于ACLT组(P<0.05)。ACLT组大鼠MMP-13 mRNA、cGAS mRNA、STING mRNA相对表达量均高于假手术组(P<0.05);电针组大鼠MMP-13 mRNA、cGAS mRNA、STING mRNA相对表达量均低于ACLT组(P<0.05)。ACLT组大鼠MMP-13、cGAS、STING蛋白相对表达量均高于假手术组(P<0.01);电针组大鼠MMP-13、cGAS、STING蛋白相对表达量均低于ACLT组(P<0.01)。结论:电针可能通过下调cGAS/STING信号通路而减轻ACLT大鼠炎症反应,延缓关节软骨退行性改变。 展开更多
关键词 前交叉韧带损伤 创伤性骨关节炎 电针 炎症反应 关节软骨 cgaS/STING信号通路 大鼠
下载PDF
人乳头瘤病毒16型E7基因靶向调控cGAS-STING信号通路抑制宫颈癌细胞免疫功能的研究
9
作者 阿尼克孜·阿不都艾尼 夏日瓦娜·阿巴斯 +2 位作者 木则帕尔·太来提 程祥 许爱敏 《中国免疫学杂志》 CAS CSCD 北大核心 2024年第2期293-298,共6页
目的:探讨HPV16 E7靶向调控cGAS-STING信号通路对宫颈癌细胞免疫功能的影响。方法:体外构建HPV16 E7过表达和干扰质粒,转染至SiHa细胞,qPCR检测HPV16 E7 mRNA表达水平,流式细胞术检测CD95、MICA/B、CD73、CD39、CTLA-4和CD25表达。在此... 目的:探讨HPV16 E7靶向调控cGAS-STING信号通路对宫颈癌细胞免疫功能的影响。方法:体外构建HPV16 E7过表达和干扰质粒,转染至SiHa细胞,qPCR检测HPV16 E7 mRNA表达水平,流式细胞术检测CD95、MICA/B、CD73、CD39、CTLA-4和CD25表达。在此基础上分别转染cGAS-siRNA和STING-siRNA至SiHa细胞,CCK8检测细胞活力,流式细胞术检测细胞凋亡。qPCR和Western blot分别检测cGAS、STING、IFN-Ⅰ、HPV16 E7的mRNA及蛋白表达。流式细胞术检测细胞CD95、MICA/B、CD73、CD39、CTLA-4和CD25表达。结果:STING-siRNA+HPV16 E7过表达组CD95和MICA/B水平降低(P<0.05),CD73、CD39、CTLA-4和CD25水平升高(P<0.05)。cGAS、STING和IFN-Ⅰ蛋白表达水平降低(P<0.05)。STINGsiRNA+HPV16 E7干扰组结果与之相反。结论:HPV16 E7靶向调控cGAS-STING信号通路进而抑制宫颈癌细胞免疫功能。 展开更多
关键词 HPV16 E7 宫颈癌 cgaS-STING 免疫功能
下载PDF
Risk Assessment of Deep-Water Horizontal X-Tree Installation 被引量:1
10
作者 MENG Wen-bo FU Guang-ming +3 位作者 HUANG Yi LIU Shu-jie HUANG Liang GAOYong-hai 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期210-220,共11页
Due to the high potential risk and many influencing factors of subsea horizontal X-tree installation,to guarantee the successful completion of sea trials of domestic subsea horizontal X-trees,this paper established a ... Due to the high potential risk and many influencing factors of subsea horizontal X-tree installation,to guarantee the successful completion of sea trials of domestic subsea horizontal X-trees,this paper established a modular risk evaluation model based on a fuzzy fault tree.First,through the analysis of the main process oftree down and combining the Offshore&Onshore Reliability Data(OREDA)failure statistics and the operation procedure and the data provided by the job,the fault tree model of risk analysis of the tree down installation was established.Then,by introducing the natural language of expert comprehensive evaluation and combining fuzzy principles,quantitative analysis was carried out,and the fuzzy number was used to calculate the failure probability of a basic event and the occurrence probability of a top event.Finally,through a sensitivity analysis of basic events,the basic events of top events significantly affected were determined,and risk control and prevention measures for the corresponding high-risk factors were proposed for subsea horizontal X-tree down installation. 展开更多
关键词 subsea horizontal X-tree risk assessment fuzzy fault tree modular risk evaluation model
下载PDF
异常流体剪切力通过cGAS-STING信号通路参与髓核细胞的凋亡、炎症与自噬
11
作者 周缘 马崇文 +3 位作者 张义宝 雷栓虎 李磊 康学文 《兰州大学学报(医学版)》 2024年第5期14-25,共12页
目的探讨异常流体剪切力诱导髓核细胞(NPC)凋亡、炎症和自噬的作用及其可能的机制。方法对NPC加载24 dyn/cm^(2)流体剪切力,时间分别为0、60、90、120、150 min。使用细胞骨架染色研究流体剪切力对细胞骨架及细胞形态的影响;使用5-乙炔... 目的探讨异常流体剪切力诱导髓核细胞(NPC)凋亡、炎症和自噬的作用及其可能的机制。方法对NPC加载24 dyn/cm^(2)流体剪切力,时间分别为0、60、90、120、150 min。使用细胞骨架染色研究流体剪切力对细胞骨架及细胞形态的影响;使用5-乙炔基-2'-脱氧尿苷染色验证NPC的增殖能力;用线粒体膜电位变化评估流体剪切力对线粒体的损伤效应;用赫斯特染色研究凋亡与流体剪切力的关系;用丹酰戊二胺染色研究自噬与流体剪切力的关系;探究细胞培养基酸碱度的变化,验证流体剪切力与炎症的关联;使用蛋白质印迹法研究cGAS-STING相关蛋白(cGAS、STING、TBK1)、炎症相关蛋白(肿瘤坏死因子-α、COX-2)、凋亡相关蛋白(Bcl-2、Bax)以及自噬蛋白(IL3-Ⅰ/Ⅱ、p62)的变化。结果作为一种细胞间的机械刺激,24 dyn/cm^(2)强度的流体剪切力可造成NPC形态由梭形转变为多边形,细胞骨架发生重组,细胞增殖能力下降,培养基酸性增强,线粒体JC-1多聚体减少、单体增加;同时,细胞内肿瘤坏死因子-α、COX-2、Bax蛋白表达量上调,LC3Ⅱ/LC3Ⅰ增加,p62、Bcl-2降解增加,出现凋亡、炎症、自噬以及线粒体损伤;加载24 dyn/cm^(2)的流体剪切力可以激活NPC内的cGAS-STING信号通路,且与时间呈正相关关系,120 min时强度最高。使用cGAS抑制剂RU.521可以减缓24 dyn/cm^(2)流体剪切力造成的NPC的凋亡、炎症、自噬以及线粒体损伤。结论流体剪切力诱导的NPC凋亡、炎症和自噬与cGAS-STING信号通路激活相关,抑制cGAS-STING信号通路的激活可以缓解异常流体剪切力造成的细胞损害。 展开更多
关键词 cgaS-STING信号通路 髓核细胞 机械刺激 流体剪切力
下载PDF
cGAS-STING通路调控NCOA4介导的铁自噬在HT22细胞缺氧复氧损伤中的作用
12
作者 赵蓝 李镇文 +2 位作者 钟振通 詹丽英 高文蔚 《疑难病杂志》 CAS 2024年第3期360-364,共5页
目的探讨环鸟苷酸—腺苷酸合成酶(cGAS)-干扰素基因刺激蛋白(STING)通路调控核受体共激活因子4(NCOA4)介导的铁自噬在小鼠海马神经细胞(HT22)缺氧复氧损伤中的作用。方法2020年9月—2022年12月于武汉大学人民医院中心实验室进行实验。将... 目的探讨环鸟苷酸—腺苷酸合成酶(cGAS)-干扰素基因刺激蛋白(STING)通路调控核受体共激活因子4(NCOA4)介导的铁自噬在小鼠海马神经细胞(HT22)缺氧复氧损伤中的作用。方法2020年9月—2022年12月于武汉大学人民医院中心实验室进行实验。将HT22细胞系随机分为4组,对照组(Ctrl组)、缺氧复氧组(HR组)、缺氧复氧+cGAS抑制剂(RU.521)组(HR+RU组)、缺氧复氧+RU.521+NCOA4过表达组(HR+RU+NCOA4组)。HR组于无糖缺氧条件下培养6 h再复糖复氧培养24 h构建缺氧复氧模型,HR+RU组及HR+RU+NCOA4组提前给予3.0μmol/L的RU.521处理24 h后构建缺氧复氧模型,HR+RU+NCOA4组于缺氧复氧前5 d给予NCOA4慢病毒转染,对照组常规培养。免疫荧光检测各组ROS,ELISA检测CCK8、LDH、SOD、MDA及亚铁离子,Western blot检测cGAS、STING、NCOA4、LC3B和铁蛋白。结果与Ctrl组比较,HR组细胞活力CCK8、SOD降低,LDH、ROS、MDA、cGAS、STING、NCOA4、LC3B、铁蛋白、亚铁离子升高(P<0.05);与HR组比较,HR+RU组细胞活力CCK8、SOD、铁蛋白升高,LDH、ROS、MDA、cGAS、STING、NCOA4、LC3B、亚铁离子降低(P<0.05);与HR+RU组比较,HR+RU+NCOA4组细胞活力CCK8、SOD、铁蛋白降低(P<0.05),LDH、ROS、MDA、NCOA4、LC3B、亚铁离子升高(P<0.05),cGAS、STING差异无统计学意义(P>0.05)。结论缺氧复氧后HT22细胞cGAS-STING通路上调,NCOA4介导的铁自噬过度激活进而加重细胞损伤。 展开更多
关键词 铁自噬 缺氧复氧 环鸟苷酸—腺苷酸合成酶—干扰素基因刺激蛋白通路 核受体共激活因子4 小鼠
下载PDF
雷公藤甲素调节cGAS/STING信号通路对坐骨神经痛大鼠的改善作用及机制
13
作者 闫改霞 林淑霞 +2 位作者 孟燕 张慧宇 景彦林 《中国药房》 CAS 北大核心 2024年第13期1594-1599,共6页
目的 探究雷公藤甲素(TP)对坐骨神经痛大鼠的改善作用及机制。方法 制备坐骨神经痛大鼠模型,然后将大鼠随机分为模型组(生理盐水),吲哚美辛组(阳性对照,7.5 mg/kg),低、高剂量TP干预组(分别记为TP-L组、TP-H组,50、100μg/kg TP)和高剂... 目的 探究雷公藤甲素(TP)对坐骨神经痛大鼠的改善作用及机制。方法 制备坐骨神经痛大鼠模型,然后将大鼠随机分为模型组(生理盐水),吲哚美辛组(阳性对照,7.5 mg/kg),低、高剂量TP干预组(分别记为TP-L组、TP-H组,50、100μg/kg TP)和高剂量TP+干扰素基因刺激因子(STING)激活剂干预组(TP-H+DMXAA组,100μg/kg TP+25 mg/kg DMXAA),每组12只;另取12只不结扎的大鼠作为假手术组(生理盐水)。腹腔注射给药/生理盐水14 d后,检测大鼠机械刺激缩足反射阈值(PWT)、热刺激缩足反射潜伏期(PWL);观察大鼠坐骨神经病理学改变、坐骨神经元形态及小胶质细胞数;检测大鼠坐骨神经中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平及环磷酸鸟苷-腺苷酸合成酶(cGAS)、STING mRNA及其蛋白表达。结果 与假手术组比较,Model组大鼠PWT、PWL均显著降低/缩短(P<0.05),尼氏体数量显著减少(P<0.05);小胶质细胞数、坐骨神经病理学评分,IL-1β、TNF-α水平,cGAS、STING mRNA及其蛋白表达水均显著增加/升高(P<0.05);坐骨神经损伤严重。与Model组比较,吲哚美辛组、TP-L组、TP-H组大鼠各指标变化与上述相反(P<0.05),坐骨神经损伤减轻。STING激活剂DMXAA减弱了TP对坐骨神经痛大鼠小胶质细胞活性和炎症反应的抑制作用(P<0.05)。结论 TP可能通过下调cGAS/STING信号通路,降低小胶质细胞活性和炎症反应,从而改善大鼠坐骨神经痛。 展开更多
关键词 雷公藤甲素 坐骨神经痛 cgaS/STING信号通路 小胶质细胞 炎症反应
下载PDF
cGAS-STING信号通路在系统性红斑狼疮中的研究进展
14
作者 苏雅珍 李瑞娇 +1 位作者 刘凡铖 张莉芸 《中华临床免疫和变态反应杂志》 CAS 2024年第2期169-173,共5页
cGAS-STING信号通路在免疫反应调控中发挥着至关重要的作用,其通过感知细胞质内DNA的存在,激活下游一系列分子,导致Ⅰ型干扰素等炎症细胞因子的释放,最终参与和调控免疫反应的发生。SLE发病机制复杂,免疫功能紊乱及Ⅰ型干扰素等细胞因... cGAS-STING信号通路在免疫反应调控中发挥着至关重要的作用,其通过感知细胞质内DNA的存在,激活下游一系列分子,导致Ⅰ型干扰素等炎症细胞因子的释放,最终参与和调控免疫反应的发生。SLE发病机制复杂,免疫功能紊乱及Ⅰ型干扰素等细胞因子参与SLE的发生发展。本文将就cGAS-STING信号通路在SLE中的研究进展进行综述,以期为SLE的诊断及靶向治疗提供新方向。 展开更多
关键词 cgaS-STING信号通路 系统性红斑狼疮 干扰素 免疫反应 靶向治疗
下载PDF
Lead, Zinc and Iron Pollutants Load Assessment in Selected Rivers in Southern Nigeria: Implications for Domestic Uses
15
作者 Ochuko Ushurhe Ozabor Famous +1 位作者 Ezekiel Ovuokerie Gunn Sapere-Obi Martha Ladebi 《Journal of Water Resource and Protection》 CAS 2024年第1期58-82,共25页
The aim of the study is to comparatively assess the concentrations of lead, zinc and iron in Rivers Ase, Warri and Ethiope, in Nigeria. Monthly water samples were collected from six randomly selected sites along the r... The aim of the study is to comparatively assess the concentrations of lead, zinc and iron in Rivers Ase, Warri and Ethiope, in Nigeria. Monthly water samples were collected from six randomly selected sites along the rivers course. 72 water samples were collected from each river at 0 - 15 cm depths. Samples were analysed based on the standard methods recommended by the WHO for testing lead, zinc and iron. The assessment of the water quality was done using the Water Quality Index (WQI) of the Canadian Council of Ministers of the Environment (CCME-WQI). While hypotheses were tested using ANOVA. Findings indicated that CCME-WQI values were 47.3, 66.52 and 78.7. This meant that the water quality of River Ase is impaired and departed from desirable levels, while that of Warri and Ethiope were considered to occasionally be impaired and depart from desirable levels. The ANOVA model showed that there is a significant variation in heavy metal load in the selected rivers at P < 0.05. River water was put to domestic uses such as drinking (20.5%) preparing food (17.8%), bathing (19.8%), washing clothes and dishes (21.3%), brushing teeth (13.3%), and catering for domestic animals (7.5%). Poverty (49.5%) was the major reason for the use of river water for domestic purposes. The locals highlighted that they usually suffer from cholera (26.8%), diarrhoea (25.8%), dysentery (24%) and typhoid (23.5%) as a result of using the river water. The study recommended routine monitoring of anthropogenic and geologic activities, testing of the water regularly amongst others. 展开更多
关键词 assessment Water-Quality-Index DOMESTIC Heavy-Metals
下载PDF
Development of Localized Assessment of Municipal Wastewater Disposal Risks
16
作者 Frederick Bloetscher Daniel E. Meeroff Brittanney Adelmann 《Journal of Water Resource and Protection》 CAS 2024年第6期395-413,共19页
A means to develop a comparative assessment of the risks of available wastewater effluent disposal options on a local scale needs to be developed to help local decision-makers make decisions on options such as direct ... A means to develop a comparative assessment of the risks of available wastewater effluent disposal options on a local scale needs to be developed to help local decision-makers make decisions on options such as direct or indirect potable reuse options. These options have garnered more interest as a result of water supply limitations in many urban areas. This risk assessment was developed from a risk assessment developed at the University of Miami in 2001 and Florida Atlantic University (FAU) in 2023. Direct potable reuse and injection wells were deemed to have the lowest risk in the most recent study by FAU. However, the injection well option may not be available everywhere. As a result, a more local means to assess exposure risk is needed. This paper outlines the process to evaluate the public health risks associated with available disposal alternatives which may be very limited in some areas. The development of exposure pathways can help local decision-makers define the challenges, and support later expert level analysis upon which public health decisions are based. 展开更多
关键词 Potable Reuse WASTEWATER Effluent Disposal Risk Risk assessment
下载PDF
A risk assessment method considering risk attributes and work safety informational needs and its application
17
作者 Cong Luo Yunsheng Zhao Ke Xu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第4期253-262,共10页
The technological revolution has spawned a new generation of industrial systems,but it has also put forward higher requirements for safety management accuracy,timeliness,and systematicness.Risk assessment needs to evo... The technological revolution has spawned a new generation of industrial systems,but it has also put forward higher requirements for safety management accuracy,timeliness,and systematicness.Risk assessment needs to evolve to address the existing and future challenges by considering the new demands and advancements in safety management.The study aims to propose a systematic and comprehensive risk assessment method to meet the needs of process system safety management.The methodology first incorporates possibility,severity,and dynamicity(PSD)to structure the“51X”evaluation indicator system,including the inherent,management,and disturbance risk factors.Subsequently,the four-tier(risk point-unit-enterprise-region)risk assessment(RA)mathematical model has been established to consider supervision needs.And in conclusion,the application of the PSD-RA method in ammonia refrigeration workshop cases and safety risk monitoring systems is presented to illustrate the feasibility and effectiveness of the proposed PSD-RA method in safety management.The findings show that the PSD-RA method can be well integrated with the needs of safety work informatization,which is also helpful for implementing the enterprise's safety work responsibility and the government's safety supervision responsibility. 展开更多
关键词 Risk assessment Safey “51X”evaluation indicator system Four-tier risk assessment model Risk attributes Process system
下载PDF
Air Quality Assessment of Ubeji Community near Petroleum-Related Activities
18
作者 Adedoyin Olubunmi Bankole Akinyemi Olufemi Ogunkeyede +5 位作者 Oluwafunmilayo Mary Haruna Harrison Agboro Prince Alex Ekhorutomwen Osasenaga Israel Otuomagie Christabel Ifuwe Efe Jeffery Isukuru 《Open Journal of Air Pollution》 2024年第2期57-71,共15页
The escalating global concern over air pollution requires rigorous investigations. This study assesses air quality near residential areas affected by petroleum-related activities in Ubeji Community, utilizing Aeroqual... The escalating global concern over air pollution requires rigorous investigations. This study assesses air quality near residential areas affected by petroleum-related activities in Ubeji Community, utilizing Aeroqual handheld mobile multi-gas monitors and air quality multi-meters. Air sampling occurred on three distinct days using multi-gas monitors and meters, covering parameters such as CO, NO2, CH4, NH3, VOCs, Particulate Matter, Temperature, Relative Humidity, and Air Quality Index. Soil and plant samples were collected and analyzed for physicochemical and organic components. Air pollutant concentrations showed significant fluctuations. Carbon monoxide (CO) ranged from 0.00 to 3.22 ppm, NO2 from 0.00 to 0.10 ppm, CH4 from 4.00 to 2083 ppm, NH3 from 371 to 5086 ppm, and VOCs from 414 to 6135 ppm. Soil analysis revealed low total nitrogen, and undetected BTEX levels. Plant samples displayed a pH range of 7.72 to 9.45. CO concentrations, although below WHO limits, indicated potential vehicular and industrial influences. Fluctuations in NO2 and CH4 were linked to traffic, industrial activities, and gas flaring. NH3 levels suggested diverse pollution sources. The result in this study highlights the dynamic nature of air pollution in Ubeji community, emphasizing the urgent need for effective pollution control measures. Although CO concentrations were within limits, continuous monitoring is essential. Elevated NO2 levels gave information on the impact of industrial activities, while high CH4 concentrations may be associated with gas flaring and illegal refining. The study recommends comprehensive measures and collaborative efforts to address these complex issues, safeguarding both the environment and public health. This study shows the potential synergy between air quality sensors and plants for holistic environmental health assessments, offering valuable insights for environmental assessments and remediation endeavours. The findings call for stringent regulations and collaborative efforts to address air pollution in Ubeji community comprehensively. 展开更多
关键词 Air Quality assessment BIOINDICATORS Ubeji Community Pollution assessment
下载PDF
Comparative study of different machine learning models in landslide susceptibility assessment: A case study of Conghua District, Guangzhou, China
19
作者 Ao Zhang Xin-wen Zhao +8 位作者 Xing-yuezi Zhao Xiao-zhan Zheng Min Zeng Xuan Huang Pan Wu Tuo Jiang Shi-chang Wang Jun He Yi-yong Li 《China Geology》 CAS CSCD 2024年第1期104-115,共12页
Machine learning is currently one of the research hotspots in the field of landslide prediction.To clarify and evaluate the differences in characteristics and prediction effects of different machine learning models,Co... Machine learning is currently one of the research hotspots in the field of landslide prediction.To clarify and evaluate the differences in characteristics and prediction effects of different machine learning models,Conghua District,which is the most prone to landslide disasters in Guangzhou,was selected for landslide susceptibility evaluation.The evaluation factors were selected by using correlation analysis and variance expansion factor method.Applying four machine learning methods namely Logistic Regression(LR),Random Forest(RF),Support Vector Machines(SVM),and Extreme Gradient Boosting(XGB),landslide models were constructed.Comparative analysis and evaluation of the model were conducted through statistical indices and receiver operating characteristic(ROC)curves.The results showed that LR,RF,SVM,and XGB models have good predictive performance for landslide susceptibility,with the area under curve(AUC)values of 0.752,0.965,0.996,and 0.998,respectively.XGB model had the highest predictive ability,followed by RF model,SVM model,and LR model.The frequency ratio(FR)accuracy of LR,RF,SVM,and XGB models was 0.775,0.842,0.759,and 0.822,respectively.RF and XGB models were superior to LR and SVM models,indicating that the integrated algorithm has better predictive ability than a single classification algorithm in regional landslide classification problems. 展开更多
关键词 Landslides susceptibility assessment Machine learning Logistic Regression Random Forest Support Vector Machines XGBoost assessment model Geological disaster investigation and prevention engineering
下载PDF
Nutritional Assessment Tools for Patients with Cancer:A Narrative Review
20
作者 Peng-peng WANG Kim Lam Soh +4 位作者 Huzwah binti Khazaai Chuan-yi NING Xue-ling HUANG Jia-xiang YU Jin-lian LIAO 《Current Medical Science》 SCIE CAS 2024年第1期71-80,共10页
Cancer patients are at high risk of malnutrition,which can lead to adverse health outcomes such as prolonged hospitalization,increased complications,and increased mortality.Accurate and timely nutritional assessment p... Cancer patients are at high risk of malnutrition,which can lead to adverse health outcomes such as prolonged hospitalization,increased complications,and increased mortality.Accurate and timely nutritional assessment plays a critical role in effectively managing malnutrition in these patients.However,while many tools exist to assess malnutrition,there is no universally accepted standard.Although different tools have their own strengths and limitations,there is a lack of narrative reviews on nutritional assessment tools for cancer patients.To address this knowledge gap,we conducted a non-systematic literature search using PubMed,Embase,Web of Science,and the Cochrane Library from their inception until May 2023.A total of 90 studies met our selection criteria and were included in our narrative review.We evaluated the applications,strengths,and limitations of 4 commonly used nutritional assessment tools for cancer patients:the Subjective Global Assessment(SGA),Patient-Generated Subjective Global Assessment(PG-SGA),Mini Nutritional Assessment(MNA),and Global Leadership Initiative on Malnutrition(GLIM).Our findings revealed that malnutrition was associated with adverse health outcomes.Each of these 4 tools has its applications,strengths,and limitations.Our findings provide medical staff with a foundation for choosing the optimal tool to rapidly and accurately assess malnutrition in cancer patients.It is essential for medical staff to be familiar with these common tools to ensure effective nutritional management of cancer patients. 展开更多
关键词 CANCER MALNUTRITION NUTRITION nutritional assessment TOOL
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部