Microsatellite instability (MSI) is used as a molecular marker for defective DNA mismatch repair (MMR) genes.We report here alterations of MSI in 15 malignant astrocytomas (WHO grade Ⅲ) and glioblastomas (GBM; WHO gr...Microsatellite instability (MSI) is used as a molecular marker for defective DNA mismatch repair (MMR) genes.We report here alterations of MSI in 15 malignant astrocytomas (WHO grade Ⅲ) and glioblastomas (GBM; WHO grade Ⅳ) of pediatric patients (2-21 years) and 12 GBM from adults (44-68 years) by comparative analysis of BAT25/BAT26 loci and 10 other microsatellite markers. High-level microsatellite instability (MSI-H) occurred in 4 of the 15 pediatric cases (26.7%) and in 1 of the 12 adult GBM cases (8.3%). Low-level mi-展开更多
Objective： To study the expressions of FAK and Pyk2 in human astrocytic tumors and their relationship with angiogenesis. Methods： The S-P immunohistochemical method was used to measure the expressions of FAK, Pyk2 a...Objective： To study the expressions of FAK and Pyk2 in human astrocytic tumors and their relationship with angiogenesis. Methods： The S-P immunohistochemical method was used to measure the expressions of FAK, Pyk2 and VEGF proteins in 58 human brain astrocytic tumors, and microvessel density （MVD） was detected by CD31 staining. Results： In astrocytic tumors with Ⅰ, Ⅱ, Ⅲ and Ⅳ grades, the positive rates of FAK were 20.00%, 26.67%, 44.44% and 50.00%, respectively, those of Pyk2 were 40.00%, 60.00%, 77.78% and 85.00%, respectively. FAK and Pyk2 expressions, especially Pyk2, correlated positively with VEGF expression and MVD. Conclusion： FAK and Pyk2 plays the important role in astrocytic tumor angiogenesis through regulation to VEGF.展开更多
文摘Microsatellite instability (MSI) is used as a molecular marker for defective DNA mismatch repair (MMR) genes.We report here alterations of MSI in 15 malignant astrocytomas (WHO grade Ⅲ) and glioblastomas (GBM; WHO grade Ⅳ) of pediatric patients (2-21 years) and 12 GBM from adults (44-68 years) by comparative analysis of BAT25/BAT26 loci and 10 other microsatellite markers. High-level microsatellite instability (MSI-H) occurred in 4 of the 15 pediatric cases (26.7%) and in 1 of the 12 adult GBM cases (8.3%). Low-level mi-
基金the Science Research Foundation of Health Department of Hubei Province (No. JX1B019)the Science Research Foundation of Wuahn University (No. 301270064)
文摘Objective： To study the expressions of FAK and Pyk2 in human astrocytic tumors and their relationship with angiogenesis. Methods： The S-P immunohistochemical method was used to measure the expressions of FAK, Pyk2 and VEGF proteins in 58 human brain astrocytic tumors, and microvessel density （MVD） was detected by CD31 staining. Results： In astrocytic tumors with Ⅰ, Ⅱ, Ⅲ and Ⅳ grades, the positive rates of FAK were 20.00%, 26.67%, 44.44% and 50.00%, respectively, those of Pyk2 were 40.00%, 60.00%, 77.78% and 85.00%, respectively. FAK and Pyk2 expressions, especially Pyk2, correlated positively with VEGF expression and MVD. Conclusion： FAK and Pyk2 plays the important role in astrocytic tumor angiogenesis through regulation to VEGF.
