Analysis of the Causes of Continuous Cropping Obstacles for Atractylodes macrocephala Koidz in Pingjiang County and Its Control Methods

First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.

Advances in research on chemical constituents,pharmacological actions and processing of atractylodes macrocephala

Rhizome atractylodes macrocephalae is the dry roots of Atractylodes macrocephala Koidz.It is one of commonly used Chinese medicine.In the "Shennong′s Herbal",it was listed as the top grade.It mainly contains volatile oil,atractylenolides,atractylodes polysaccharides,glycosides and amino acids.And it has the medical functions of good for spleen and intestine,diuretic and dehumidification,hidroschesis,miscarriage prevention and soon.In order to provide references for further development and utilization,this paper systematic arranged the Chinese medicine atractylodes chemical composition,pharmacological effects,processing technology and effect of processing technology on chemical composition and pharmacological action.

Populational change of CD4^(+)CD25^(+)Treg cells is responsible for the synergistic effect of the combination of RAMP2 with baicalin in treating recurrent spontaneous abortion mouse models

Background: The absence of a safe and effective therapy for recurrent spontaneous abortion due to a maternofetal failure in immunological tolerance remains an intractable clinical obstacle for surgeons. Recently, traditional Chinese medicine has become a feasible alternative for certain diseases, including recurrent spontaneous abortion. However, because of the complex composition of the traditional Chinese medicine formula, its action mechanism remains unclear. Methods: We selected two isolated active ingredients (RAMP and baicalin) from the traditional Chinese medicine formula and used an abortion-prone CBA/J × DBA/2 model to simulate human RSA and compared the changes in fetal resorption rate, Treg cell percentage, and relevant cytokines before and after combination therapy. In addition, The mechanisms were preliminarily discussed using in vitro differentiation models. Results: In CBA/J × DBA/2 abortion-prone mice, the combination therapy resulted in a lower embryo resorption rate compared to that obtained with individual delivery of either RAMP or baicalin, thereby playing an embryo-protective role through the increase in Treg cells for the maintenance of maternal-fetal immune tolerance. In in vitro primary cell differentiation experiments, the concentration of Treg cells significantly increased from 11% to 17.9% after the combination therapy compared to that of the single administration group. Conclusion: the synergistic effects of RAMP and baicalin were responsible for Treg differentiation. The present study provides a solid basis for improving the applicability of traditional Chinese herbs in the treatment of recurrent spontaneous abortion.

白术主要病害防控技术

针对白术(Atractylodes macrocephala Koidz)近年来主要病害进行调查,介绍了病害的症状和发生规律,对选种、土壤类型、田园清洁、田间管理、土肥施用和药剂防治等防控措施进行了总结,旨在提供有效的防控技术,确保白术产量和品质安全,为白术产业的可持续发展保驾护航。

咸丰白术种子生物学特性研究

以白术(Atractylodes macrocephala Koidz.)种子为试材,通过测定形态、千粒重、生活力、吸水特性、水分含量和发芽率,探究咸丰不同地区白术种子的生物学特性以及适宜的消毒方式和激素处理,为发掘地区优势种源和开发种苗高效繁育技术奠定研究基础。结果表明,咸丰不同地区白术种子在形态、生活力和吸水特性等方面无显著差异。李子溪2的白术种子千粒重最大,活龙坪的白术种子水分含量最高,中心场的白术种子发芽率最高。使用5%高锰酸钾、3%过氧化氢、75%酒精和3%次氯酸钠进行消毒均可显著降低白术种子的霉变率,其中3%次氯酸钠消毒效果较好,且对白术种子萌发具有一定的促进作用,而75%酒精对白术种子萌发具有很强的抑制作用。相比于对照处理,100、200、400 mg/L的GA_(3)对白术种子萌发具有显著的促进作用,发芽率增幅均为20.4%。5、10、20 mg/L的IAA对白术种子的萌发有显著的促进作用,发芽率增幅分别为31.0%、25.9%和24.3%。5 mg/L和10 mg/L的6-BA对白术种子的萌发也具有显著的促进作用,相比于对照处理,发芽率增幅分别为27.1%和32.2%。综上表明,中心场的白术种子活力、发芽率和发芽势最高,可初步作为咸丰的优势种源。种子消毒处理以3%次氯酸钠浸泡15 min为宜。100~400 mg/L的GA_(3)、5~20 mg/L的IAA以及5~10 mg/L的6-BA对白术种子萌发均具有较好的促进效果。

Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant Atractylodes macrocephala

We developed an efficient plant regeneration protocol for rapidly propagating Atractylodes macrocephala Koidz,an important traditional Chinese medicinal plant,via shoot organogenesis.Shoot multiplication was induced on Murashige-Skoog(MS)medium supplemented with various concentrations of N-phenyl-N-1,2,3-thidiazol-5-ylurea(TDZ),6-benzylaminopurine(BA)andα-naphthaleneacetic acid(NAA).Rooting was induced on half-strength MS medium supplemented with NAA and indolebutyric acid(IBA).The maximum mean number of shoots(5.61)was obtained from a single explant by the combined effect of 1.08μmol/L NAA and 2.25μmol/L TDZ.The longest roots and a minimum number of roots were produced when they were cultured in a medium without plant growth regulators.The shortest roots and the largest number of roots were observed in the medium supplemented with 2.7μmol/L NAA.

白术腋芽离体快繁技术初探

以白术(Atractylodes macrocephala Koidz.)的腋芽、茎尖为外植体,添加不同浓度的6-BA、NAA等生长激素到MS、1/2 MS培养基中,对丛生芽进行分化诱导液体培养和生长培养;添加不同浓度的NAA、IBA到1/4 MS培养基中进行液体生根及壮苗培养,对驯化后的白术试管苗炼苗移栽。结果表明,诱导分化液体培养基为1/2 MS+1.5 mg/L 6-BA+0.4 mg/L NAA+0.3 mg/L IBA+0.6 mg/L GA_(3),光照时间18 h/d,光照度1800 lx,丛生芽分化诱导形成率为97.3%;最高增值倍数为16;丛生芽生根诱导液体培养基为1/4 MS+0.4 mg/L NAA+0.2 mg/L IBA,光照18 h/d,光照度1900 lx,生根率高达98.9%,平均生根数8.5条/芽;炼苗成活率95%以上。移栽苗圃成活率为94%。