Morphology prediction of dihydroxylammonium 5,5'-bistetrazole-1,1'-diolate (TKX-50) crystal in different solvent systems using modified attachment energy model

In order to theoretically study the growth morphology of dihydroxylammonium 5,5’-bistetrazole-1,1’-dio late(TKX-50)crystal in different solvent systems,crystal–solvent models were established,and then molecular dynamics(MD)methods were adopted as a means to simulate particle motion.Modified attachment energy(MAE)model was employed to calculate the growth morphology of TKX-50.The simulation results demonstrate that COMPASS force field and RESP charge are suitable for molecular dynamics simulation of TKX-50.The morphologically dominant growth surfaces of TKX-50 in vacuum are(020),(011),(11–1),(100)and(120),respectively.In water(H_(2)O)and N,N-dimethylformamide(DMF)solvents,the(11–1)face is the largest in the habit face,the growth rate of(020)face becomes faster.With the increase of temperature,the aspect ratios of TKX-50 crystal in DMF solvent increase,and the areas of the(120)faces decrease.In ethylene glycol/H_(2)O mixed solvent system with volume ratio of 1/1,aspect ratio of TKX-50 is relatively small.In formic acid/H_(2)O mixed solvents with different volume ratios(1/4,1/3,1/2,1/1 and 2/1),aspect ratio of TKX-50 is relatively small when volume ratio is 1/2.

Cytokine changes and embryo attachment in mouse endometrial cells following treated with peripheral blood mononuclear cells(PBMCs)expressing ectopic hCG,and hCG-activated PBMCs

Objective:To compare the effect of human chorionic gonadotropin(hCG)-producing peripheral blood mononuclear cells(PBMCs)and PBMCs activated by hCG in vitro and expressions of related immune genes in mouse implantation.Methods:hCG-producing PBMCs(transfected PBMC)and PBMCs activated by hCG in vitro were introduced into isolated mouse endometrial cells,while cell cultures were divided into four groups:the control,PBMC,transfected,and activated PBMC groups.The expression of studied genes(IL-1β,IL-6,Lif,and Vegf)was evaluated and blastocyst attachment on the cocultured cells(isolated endometrial cells and PBMC cells)was monitored in all four groups.Results:Data showed that expression decreased in the PBMC group compared to the treated PBMC(transfected and activated PBMCs)and increased in transfected PBMC compared to the activated PBMC.Attachment and migration of blastocysts were dramatically enhanced in the transfected PBMC group compared to the activated PBMC group(P<0.05).Conclusions:Use of hCG-producing PBMCs(transfected PBMC)has more influence on endometrial receptivity.

Relationship between Parent-Child Attachment and Problem Behaviors among Chinese Firstborn Children in Family Transitions: A Meta-Analysis

The implementation of China’s three-child fertility policy has led to a notable increase in multiple-child families.Notably,firstborn children experience a significant transition from being an only child to a non-only child.This transition is associated with problematic behaviors,affecting their social adjustment,sibling relationships,and family harmony.Although several studies have examined the relationship between parent-child attachment and problem behaviors exhibited byfirstborn children during family transitions,thefindings have been inconsistent.Hence,a meta-analytic study was undertaken to elucidate the inconsistencies in this relationship and explore the moderating factors that may contribute to these discrepancies.Using a systematic literature retrieval and screening method,12 effect sizes were derived from the 10 eligible articles,encompassing a sample size of 5319.The meta-analysis demonstrated a low negative association between parent-child secure attachment and problem behaviors exhibited byfirstborn children during family transitions.Furthermore,the present study investigates potential moderator factors,such as children’s age and geographic region,to gain a more nuanced understanding of the relationship.Consequently,the establishment of parent-child attachment relationships has the potential to mitigate problem behaviors observed infirst-born children during family transitions.The implications of thesefindings indicate that parents can nurture secure attachment bonds with their children by demonstrating sensitive responsiveness,employing positive parenting practices,and fostering emotional availability.These efforts contribute to the cultivation of secure internal working models and positive behavioral manifestations withinfirst-born children,which in turn affect their relationships with siblings.

Landscape Upgrading and Transformation of Old Urban Parks from the Perspective of Place Attachment:A Case Study of Nanchang Bayi Park

On the basis of combing the relevant theories of place attachment, the planning framework of upgrading and reconstruction of old parks based on place attachment was put forward. Taking Nanchang Bayi Park as an example, the methods and key points of landscape improvement and transformation of old urban parks based on place attachment were explained to provide new ways and references for the renewal of old urban parks.

The Evolving Bipartite Network and Semi-Bipartite Network Models with Adjustable Scale and Hybrid Attachment Mechanisms

The bipartite graph structure exists in the connections of many objects in the real world, and the evolving modeling is a good method to describe and understand the generation and evolution within various real complex networks. Previous bipartite models were proposed to mostly explain the principle of attachments, and ignored the diverse growth speed of nodes of sets in different bipartite networks. In this paper, we propose an evolving bipartite network model with adjustable node scale and hybrid attachment mechanisms, which uses different probability parameters to control the scale of two disjoint sets of nodes and the preference strength of hybrid attachment respectively. The results show that the degree distribution of single set in the proposed model follows a shifted power-law distribution when parameter r and s are not equal to 0, or exponential distribution when r or s is equal to 0. Furthermore, we extend the previous model to a semi-bipartite network model, which embeds more user association information into the internal network, so that the model is capable of carrying and revealing more deep information of each user in the network. The simulation results of two models are in good agreement with the empirical data, which verifies that the models have a good performance on real networks from the perspective of degree distribution. We believe these two models are valuable for an explanation of the origin and growth of bipartite systems that truly exist.

Anchor Attachment蛋白在结直肠癌中表达增强

目的探讨Anchor Attachment蛋白(AAP)的表达与结直肠癌浸润、转移的关系及临床意义。方法免疫组织化学法检测AAP的表达,分析AAP表达水平与肿瘤临床病理特征之间的关系;并用酶联免疫吸附(ELISA)法检测血清中AAP表达水平。结果结直肠正常肠黏膜、癌原发灶、淋巴结和肝转移灶中AAP的表达阳性率分别为20.5%、53.0%、69.8%和80.0%;癌原发灶、转移淋巴结和肝转移灶中AAP表达阳性率均显著高于正常肠黏膜组织(P<0.01),转移淋巴结和肝转移灶中AAP阳性率高于癌原发灶(P<0.05);淋巴结转移患者和肝转移患者的原发灶AAP阳性率显著高于无转移者(P<0.01);Dukes分期A、B、C、D期患者AAP阳性率逐渐增高,各分期之间差异显著(P<0.01)。83例结直肠癌患者血清AAP水平为(6.3±2.8)μg/L,显著高于30例健康志愿者的(2.2±0.9)μg/L,(t=6.976,P<0.01);Dukes分期A、B期患者血清AAP水平为(5.2±2.6)μg/L,显著低于C、D期患者的(7.1±2.9)μg/L(P<0.05)。结论检测外周静脉血AAP水平对预测和判断结直肠癌病情和肝转移有重要意义。

Evolution of Biofilm and Its Effect on Microstructure of Mortar Surfaces in Simulated Seawater

To explore the role of biofilm formation on the corrosion of marine concrete structures, we investigated the attachment of biofilm on mortar surfaces in simulated seawater and the influence of biofilm on the microstructure of mortar surfaces. The results show that the evolution of biofilm on mortar surfaces in simulated seawater is closely related to the corrosion suffered by the mortar, and the process of biofilm attachment and shedding is continuous and cyclical. It is found that the specimens in the absence of biofilm attachment are more severely eroded internally by the corrosive medium in simulated seawater than those in the presence of biofilm attachment. For the specimens without biofilm attachment, after 60 days, gypsum forms,and after 120 days, the number of pores in the mortar is reduced. In contrast, for the specimens in the presence of biofilm attachment, gypsum could only be detected after 90 days, and fewer pores are filled. Therefore, the formation of biofilm could delay the invasion of the corrosive medium into the interior of mortar during the evolution of biofilm on mortar surfaces, mitigating the corrosion of mortars in seawater.

Unravelling the role of the combined effect of metallic charge transfer channel and SiO_(x) overlayer in the Zr/Si-Fe_(2)O_(3):Au:SiO_(x) nanorod arrays to boost photoelectrochemical water splitting

Hematite(α-Fe_(2)O_(3)) based photoanodes have been extensively studied due to various intriguing features that make them viable candidates for a photoelectrochemical(PEC) water splitting photoanode.Herein,we propose a Zr-doped Fe_(2)O_(3) photoanode decorated with facilely spin-coated Au nanoparticles(NPs) and microwave-assisted attached Si co-doping in conjunction with a SiO_(x) overlayer that displayed a remarkable photocurrent density of 2.01 mA/cm^(2) at 1.23 V vs.RHE.The kinetic dynamics at the photoelectrode/-electrolyte interface was examined by employing systematic electrochemical investigations.The Au NPs played a dual role in increasing PEC water splitting.First,the Schottky interface that was formed between Au NPs and Zr-Fe_(2)O_(3) lectrode ensured the prevention of electron flow from the photoanode to the metal,increasing the number of available charges as well as suppressing surface charge recombination.Second,Au extracted photoholes from the bulk of the Zr-Fe_(2)O_(3) and transported them to the outer SiO_(x) overlayer,while the SiO_(x) overlayer efficiently collected the photoholes and promoted the hole injection into the electrolyte.Further,Si co-doping enhanced bulk conductivity by reducing bulk charge transfer resistance and improving charge carrier density.This study outlines a technique to design a metallic charge transfer path with an overlayer for solar energy conversion.

Self-adhesive pads in defi brillation or cardioversion: risks due to unfamiliarity and recommendations

During cardiopulmonary resuscitation(CPR),defibrillation,or cardioversion,the use of self-adhesive pads instead of conventional paddles has gradually become common.In particular,it was recommended during the COVID-19 pandemic,as it allows defibrillation or cardioversion to be performed without close contact between the rescuer and patient.[1,2]Self-adhesive pads can minimize the downtime of chest compressions before and after defibrillation and protect the patient from electric arc formation,skin burns,and fi re.Once the pads are attached,electrocardiographic rhythm analysis,defibrillation,synchronous cardioversion,and cardiovascular support are all possible.Unlike paddles,self-adhesive pads can save time because the application of conductive jelly,repeated positioning,and application of 10-12 kg of pressure on the chest are not needed.The European Resuscitation Council(ERC)Guidelines 2021 state that the use of self-adhesive pads is recommended in advanced cardiovascular life support(ACLS).[3]Nevertheless,some medical staff still tend to prefer the use of conventional paddles instead of self-adhesive pads in hospitals.

China-EU Ties Gather Pace amid Rumblings of De-risking and Decoupling

China-EU Summit charts the course for the development of China-EU relations with its first onsite session since 2019.D ESPITE the din about de-risking and decoupling made by some Western media,the convening of the 24th EU-China Summit in Beijing on December 7,the first in-person one since 2019,showed the significant importance the two sides attached to the bilateral relations,and their mutual intention to steer the important ties toward more fruitful cooperation.

Improving bubble-particle attachment during the flotation of low rank coal by surface modification

Based on the problems caused by many oxygen-containing functional groups and poor floatability on the surface of low rank coal,the characteristics of low rank coal were analyzed systematically by means of scanning electron microscopy(SEM),X-ray diffraction(XRD)and X-Ray photoelectron spectroscopy(XPS)techniques.The bubble-particle induction time was used to determine the characterization of the bubble-particle attachment,and the bubble-particle attachment of low rank coal modified by soaking the coal samples in an acid or alkaline solution was analyzed.The floatability of the modified coal surface was verified by flotation tests.The results show that the particle size of 0.125–0.074 mm of the coal sample exhibited better bubble-particle attachment characteristics.The small bubble,the larger approach velocity of bubble and the larger bubble deformation were more helpful to enhance the bubbleparticle attachment.For an acid solution,the smaller the p H was and the longer the soaking time was,the better the floatability of the coal sample and the higher the combustible material recovery were.The combustible material recovery of low rank coal was increased to 78.79%by soaking the sample in an acid solution of pH=0 for 180 min.On the contrary there was a best concentration for the alkaline solution.

Attachment Styles are Related to ERPs Elicited to Angry Faces in an Oddball Paradigm

Attachment theory suggests that anxious attachment is associated with hypervigilance to threatening social stimuli, and avoidant attachment with avoidance or suppression of processing such stimuli. Twenty-five students viewed angry, fearful and neutral female faces in four visual oddball tasks, and completed the Attachment Style Questionnaire, the Autonomy-Connectedness Scale, and Anxiety and Depression subscales of the Symptom Checklist-90. When the oddballs were angry faces in a background of neutral frequents, we found higher levels of autonomy and secure attachment to be related to larger N100 and smaller P300 amplitudes;higher levels of anxious attachment were, on the contrary, associated with smaller N100 and larger P300 amplitudes. Variation in attachment is related to approaching, or withdrawing from threatening stimuli, and ERP-techniques add to our understanding of how the attachment system actually works.

Preliminary Validation of Tumor Cell Attachment Inhibition Assay for Developmental Toxicants With Mouse S180 Cells

This study was designed to explore the possibility of using ascitic mouse sarcoma cell line (S180) to validate the mouse tumor cell attachment assay for developmental toxicants, and to test the inhibitory effects of various developmental toxicants. The results showed that 2 of 3 developmental toxicants under consideration, sodium pentobarbital and ethanol, significantly inhibited S180cells attachment to Concanavalin A-coaed surfaces. Inhibition was dependent on concentration, and the IC50 (the concentration tha reduced attachment by 50% ), of these 2 chemicals was 1.2×10-3mol/L and 1 .0 mol/L, respectively. Anoher developmental toxiant, hydmiortisone, did not show inhibitory activity. Two non-developmental toxicants, sodium chloride and glycine were also tested and these did not decrease attachment rates. The main results reported here were generally sindlar to those obtained with ascitic mouse ovdrian tumor cells as a model. Therefore, this study added further evidence to the conclusion that cell specificity does not lindt attachment inhibition to Con A-coated surfaces, so S180 cell may serve as an altemative cell model, especially when other cell lines are unavailable. Furthermore, after optimal validation, it can be suggested that an S180 cell attachment assay may be a candidate for a series of assays to detect developmental toxicants.

Adaptive Wall-Based Attachment Ventilation:A Comparative Study on Its Effectiveness in Airborne Infection Isolation Rooms with Negative Pressure

The transmission of coronavirus disease 2019(COVID-19)has presented challenges for the control of the indoor environment of isolation wards.Scientific air distribution design and operation management are crucial to ensure the environmental safety of medical staff.This paper proposes the application of adaptive wall-based attachment ventilation and evaluates this air supply mode based on contaminants dispersion,removal efficiency,thermal comfort,and operating expense.Adaptive wall-based attachment ventilation provides a direct supply of fresh air to the occupied zone.In comparison with a ceiling air supply or upper sidewall air supply,adaptive wall-based attachment ventilation results in a 15%–47%lower average concentration of contaminants,for a continual release of contaminants at the same air changes per hour(ACH;10 h^(-1)).The contaminant removal efficiency of complete mixing ventilation cannot exceed 1.For adaptive wall-based attachment ventilation,the contaminant removal efficiency is an exponential function of the ACH.Compared with the ceiling air supply mode or upper sidewall air supply mode,adaptive wall-based attachment ventilation achieves a similar thermal comfort level(predicted mean vote(PMV)of0.1–0.4;draught rate of 2.5%–6.7%)and a similar performance in removing contaminants,but has a lower ACH and uses less energy.

THE CORROSIVE EFFECT OF BARNACLES ON LOW ALLOY STEELS

The effect of barnacles on corrosion of low alloy (16Mn) steel was studied by immersing steel panels in seawater at Zhonggang.Qingdao, for 41 months. It was found that the secondary attachment on this kind of steel by barnacles had less effect on its corrosion while the primary attachment had a greater effect; that different seasons have different effects on the form and extent of local corrosion in this steel, and that primarily attached barnacles cause more serious local corrosion in high breeding seasons when there are more attachments.

Nonculturability of the pathogenic Vibrio parahaemolyticus in live culture of Grateloupia turuturu is associated with bacterial attachment to the algal thalli

The invasive red alga Grateloupia turuturu Yamada could turn Vibrio parahaemolyticus into nonculturable state in live algal culture.In order to elucidate the mechanism of such an effect,a series of culture experiments were performed in this investigation based on three hypothesized causes,namely bacterial attachment,production of reactive oxygen species （ROS） and the discharge of water soluble secondary metabolic compounds.The results reveal that attachment to the thallus surface of G.turuturu was the major reason for the decrease of V.parahaemolyticus in seawater.Further investigations show that V.parahaemolyticus attachment to the surface of algal thallus in live cultures of seaweeds was a common phenomenon.However,the disappearance of the culturability of V.parahaemolyticus occurred only on the thallus of G.turuturu over 72 h among all six algal species tested.Electron microscopic scanning shows that most of V.parahaemolyticus attached to G.turuturu changed from the initial normal bacilli to coccoid-shape after 72 h.The enclosure experiments by enclosing the algal thallus in tubes demonstrate that the nonculturability of V.parahaemolyticus in the water of live culture of G.turuturu occurred after the physical contact of the V.parahaemolyticus to the alga.The capacity of G.turuturu in affecting the culturability of V.parahaemolyticus was not influenced after inhibition of photosynthesis by treatment of 3（3,4dichlorophenyl）-1 ,1dimethyl urea （DCMU） at non-lethal levels.Production of reactive oxygen species after addition of live culture of bacteria was excluded by on-line analyzing the oxidation of dichlorohydrofluorescein （DCFH） to dichlorofluorescein （DCF） in the presence of peroxidase on a VersaFluor fluorometer.

Effect of the surface microstructure of arsenopyrite on the attachment of Sulfobacillus thermosulfidooxidans in the presence of dissolved As(Ⅲ)

Understanding bacterial adsorption and the evolution of biofilms on arsenopyrite with different surface structures is of great signific-ance to clarifying the mechanism of microbe-mineral interfacial interactions and the production of acidic mine drainage impacting the environ-ment.In this study,the attachment of Sulfobacillus thermosulfidooxidans cells and subsequent biofilm formation on arsenopyrite with different surface structures in the presence of dissolved As(Ⅲ)was studied.Arsenopyrite slices with a specific surface were obtained by electrochemic-al corrosion at 0.26 V.The scanning electronic microscopy-energy dispersion spectra analyses indicated that the arsenopyrite surface deficient in sulfur and iron obtained by electrochemical treatment was not favorable for the initial adsorption of bacteria,and the addition of As(Ⅲ)in-hibited the adsorption of microbial cells.Epifluorescence microscopy showed that the number of cells attaching to the arsenopyrite surface in-creased with time;however,biofilm formation was delayed significantly when As(Ⅲ)was added.

Contribution of attachment insecurity to health-related quality of life in depressed patients

AIM: To examine the individual contributions of insecure attachment styles and depression symptom severity to health-related quality of life(HRQo L) in patients diagnosed with adjustment disorder(AJD) with depressed mood. METHODS: Participants were 67 patients diagnosed with International Classification of Diseases, Tenth edition AJD with depressed mood, who completed standardised self-report questionnaires measuring study variables. Mean scores and SDs were computed for the outcome and predictor measures. Pearson correlations among the measures were computed. The study hypotheses were tested using analysis of variance(ANOVA) and multiple regression analyses. All analyses were performed using the SPSS-17 software package(SPSS Inc., Chicago, IL, United States).RESULTS: ANOVA showed a significant main effect of the insecure attachment styles on depression symptom severity and life satisfaction scores. The results suggestthat depressive symptoms were more severe(F = 4.13, df = 2.67, P < 0.05) and life satisfaction was poorer(F = 5.69, df = 2.67, P < 0.01) in both anxious-ambivalently and avoidantly attached patients compared with their securely attached counterparts, whereas the two insecure groups did not significantly differ by these variables. The anxious/ambivalent attachment style and depression symptom severity significantly contributed to HRQo L, accounting for 21.4% and 29.7% of the total variance, respectively [R2 = 0.79; Adjusted R2 = 0.77; F(5, 67) = 33.68, P < 0.0001], even after controlling for gender, marital and employment status confounders.CONCLUSION: The results show that the anxious/ambivalent attachment style together with depression symptom severity substantially and independently predict the HRQo L outcome in AJD with depressed mood.

Zoospores of Undaria pinnatifida:their efficiency to attach under different water velocities and conjugation behavior during attachment

In the invading course of Undaria pinnatifida, zoospore attachment in a dynamically changed subtidal water environment is crucial for the establishment of a potential population in alien waters. Among many abiotic factors that may interfere with the attachment process, water velocity is the most important one. In this investigation, the effect of water velocity on zoospore attachment of U. pinnatifida was investigated in an artificially designed system. It was found that freshly released zoospores that were transported by water flowing at 0 -16 cm/s showed no difficulty in attaching the smooth surface. Zoospore attachment decreased at elevated water flowing rates. At 70 cm/s no spore attachment occurred. Spores that have settled on glass slide for up to 1 h could not be stripped away by flowing water at a rate of 129 cm/s, the same was true of the 20 d old filamentous gametophytes. It was found that more than 70% of free-swimming zoospores tended to settle down adjacent to the settled spores and formed conjugated clusters from two up to a few hundred cells in still culture.

The Role of Internet Pornography Use and Cyber Infidelity in the Associations between Personality, Attachment, and Couple and Sexual Satisfaction

Although adults in romantic relationships currently show more openness to online sexual behaviors [1], these behaviors can nevertheless increase couple conflicts and instability [2]. In the current study, we assess the mediating role of Internet pornography use and cyber infidelity in the relationship between 1) personality and attachment, and 2) couple and sexual satisfaction. A total of 779 participants in couple relationships (average age = 29.9 years) completed a series of online questionnaires. According to their responses, 65% of participants visited an adult site at least once during the six months preceding the study, while 16.3% did so multiple times per week. Path model results showed that Internet pornography use and cyber infidelity were sequential mediators between, on the one hand, personality and attachment, and on the other hand, couple and sexual satisfaction. The discussion highlights the importance of properly documenting the correlates of online sexual behavior to better understand new couple realities and dynamics.