Existing Problems and Solutions on Rural Primary School Language Education

At present,there are still many problems in language teaching in rural primary schools,which will affect the quality of teaching if we don't pay much attention to them.This article focuses on the existing flaws in current language teaching and provides some solutions.

A Simple Primary Key Algorithm Based Shade Dispersion Method for Maximizing PV Power Generation under Partial Shading Conditions

The output power generation of a photovoltaic(PV)array reduces under partial shading,resulting in multiple local maxima in the PV characteristics and inaccurate tracking of the global maximum power point(GMPP).Various interconnection schemes are available to reduce power losses under partial shading.In this study,a primary key algorithm is proposed for distributing shading across an array.This method is suitable for any n×n PV array configuration and involves fewer calculations and variables,leading to reduced computational complexity.The power generations of a 9×9 PV array under four different shading conditions were compared with the configurations of:total cross-tied(TCT)and Su Du Ku,physical relocation and fixed column position of modules with fixed electrical connection(PRFCPM-FEC),and magic square(MS)and improved-odd-even-prime(IOEP).The advantage of the proposed method is that once the primary key elements are obtained,the remaining array elements are numbered in a simpler manner.The results obtained using the proposed arrangement show that the power is enhanced with reference to the TCT and is comparable to the Su Do Ku,PRFCPM-FEC,MS,and IOEP reconfigurations.

A 3D Human Liver Model of Nonalcoholic Steatohepatitis

Background and Aims: To better understand nonalcoholic steatohepatitis (NASH) disease progression and to evaluate drug targets and compound activity, we undertook the devel-opment of an in vitro 3D model to mimic liver architecture and the NASH environment. Methods:We have developed an in vitro preclinical 3D NASH model by coculturing primary hu-man hepatocytes, human stellate cells, liver endothelial cells and Kupffer cells embedded in a hydrogel of rat collagen on a 96-well plate. A NASH-like environment was induced by ad-dition of medium containing free fatty acids and tumor ne-crosis factor-α. This model was then characterized by biochemical, imaging and transcriptomics analyses. Results:We succeeded in defining suitable culture conditions to main-tain the 3D coculture for up to 10 days in vitro, with the lowest level of steatosis and reproducible low level of inflammation and fibrosis. NASH disease was induced with a custom me-dium mimicking NASH features. The cell model exhibited the key NASH disease phenotypes of hepatocyte injury, steatosis, inflammation, and fibrosis. Hepatocyte injury was highlighted by a decrease of CYP3A4 expression and activity, without loss of viability up to day 10. Moreover, the model was able to stimulate a stable inflammatory and early fibrotic environ-ment, with expression and secretion of several cytokines. A global gene expression analysis confirmed the NASH induc-tion. Conclusions:This is a new in vitro model of NASH dis-ease consisting of four human primary cell-types that exhibits most features of the disease. The 10-day cell viability and cost effectiveness of the model make it suitable for medium throughput drug screening and provide attractive avenues to better understand disease physiology and to identify and characterize new drug targets.