Objective. To investigate the roles of mouse erythroid differentiation and denucleation factor (MKI)DF), a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation.Method. Mouse erythroleu...Objective. To investigate the roles of mouse erythroid differentiation and denucleation factor (MKI)DF), a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation.Method. Mouse erythroleukemia (MEL) cells were transfected with eukaryotie expression plasinid pcl)-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and p-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitolic index,and colony-forming rate in semi-solid medium ( P<0. 01). The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells trarisfected with pcDNA-MEDDF was 3. 43 times higherthan that of control (MEL transfected with blank vector, pcDNA3. 1), and the expression of c-rnyc decreased by66. 3% .Conclusions. MEDDF can induce differentiation of MEL cell and suppress its malignancy.展开更多
在高真空下 ,研究了净化剂B2 O3,79%SiO2 + 1 2 .5%B2 O3+ 2 .2 %Al2 O3+ 0 .6%CaO + 5.7%Na2 O(简写为Na Ca Al B Si)和 50 %Na Ca Al B Si+ 50 %Na2 B7O4对Cu50 Ni50 合金熔体在循环过热过程中的过冷度及其稳定性的影响。结果表明 ,B2...在高真空下 ,研究了净化剂B2 O3,79%SiO2 + 1 2 .5%B2 O3+ 2 .2 %Al2 O3+ 0 .6%CaO + 5.7%Na2 O(简写为Na Ca Al B Si)和 50 %Na Ca Al B Si+ 50 %Na2 B7O4对Cu50 Ni50 合金熔体在循环过热过程中的过冷度及其稳定性的影响。结果表明 ,B2 O3的净化过程为纯物理净化 ,合金熔体在循环过热过程中不能获得稳定的深过冷 ;Na Ca Al B Si玻璃的净化过程为物理 -化学复合净化 ,但由于该净化剂粘度大 ,在循环过热的冷却过程中因发生合金熔体与净化剂分离 ,使合金熔体表面氧化 ,导致合金熔体同样不能获得稳定深过冷 ;50 %Na Ca Al B Si+ 50 %Na2 B7O4玻璃的净化过程为物理 -化学复合净化 ,该净化剂粘度适中 ,合金熔体在循环过热过程中可以获得稳定深过冷。展开更多
基金This work supported by the National Natural Sciences Foundation of China(39670364)This work was originally published in Acta Academiae Medicinae Sinicae(200123: 32-35)in Chinese.
文摘Objective. To investigate the roles of mouse erythroid differentiation and denucleation factor (MKI)DF), a novel factor cloned in our laboratory recently, in erylhroid terminal differentiation.Method. Mouse erythroleukemia (MEL) cells were transfected with eukaryotie expression plasinid pcl)-NA-MEDDF. Then we investigated the changes on characteristics of cell growth by analyzing cells growth rate, mitotie index and colony-forming rate in semi-solid medium. The expressions of c-mye and p-globin genes were analysed by semi-quantitative RT-PCR.Results. MEL cells transfected with pcDNA-MEDDF showed significant lower growth rate, mitolic index,and colony-forming rate in semi-solid medium ( P<0. 01). The percentage of benzidine-positive cells was 32. 8%after transfection. The expression of β-globin in cells trarisfected with pcDNA-MEDDF was 3. 43 times higherthan that of control (MEL transfected with blank vector, pcDNA3. 1), and the expression of c-rnyc decreased by66. 3% .Conclusions. MEDDF can induce differentiation of MEL cell and suppress its malignancy.
文摘在高真空下 ,研究了净化剂B2 O3,79%SiO2 + 1 2 .5%B2 O3+ 2 .2 %Al2 O3+ 0 .6%CaO + 5.7%Na2 O(简写为Na Ca Al B Si)和 50 %Na Ca Al B Si+ 50 %Na2 B7O4对Cu50 Ni50 合金熔体在循环过热过程中的过冷度及其稳定性的影响。结果表明 ,B2 O3的净化过程为纯物理净化 ,合金熔体在循环过热过程中不能获得稳定的深过冷 ;Na Ca Al B Si玻璃的净化过程为物理 -化学复合净化 ,但由于该净化剂粘度大 ,在循环过热的冷却过程中因发生合金熔体与净化剂分离 ,使合金熔体表面氧化 ,导致合金熔体同样不能获得稳定深过冷 ;50 %Na Ca Al B Si+ 50 %Na2 B7O4玻璃的净化过程为物理 -化学复合净化 ,该净化剂粘度适中 ,合金熔体在循环过热过程中可以获得稳定深过冷。
