Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% d...Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% decolorization of Reactive blue 160 (RB160), Reactive black 5 (RB5) and Amido black 10B (AB10B) respectively. Further analysis using UV-vis, HPLC, and FTIR, <sup>1</sup>H NMR had shown the degradation of the dyes. Toxicity analysis of the metabolites was performed using seed germination and plant growth on two agriculturally important plants Guar (Cyamopsis tetragonoloba) and wheat (Triticum aestivum) as well as cytotoxicity analysis using the human keratinocyte cell line (HaCaT). The dye mix appeared inhibitory for seed germination (20% - 40%), whereas metabolites were non-inhibitory for germination. Treatment of HaCaT cells with of dye mix and metabolites led into 45% and ~100% of cell viability of HaCaT cells respectively. Therefore, metabolites following degradation of the dye mix were observed to be non-toxic.展开更多
目的 探讨近红外自体荧光显像(NIRAF)技术辅助识别甲状旁腺的有效性及对术后甲状旁腺保护和甲状旁腺激素表达的影响。方法 选择2020年1月至2022年9月在邢台医学高等专科学校第二附属医院收治的122例甲状腺癌患者,其中男性95例,女性27例...目的 探讨近红外自体荧光显像(NIRAF)技术辅助识别甲状旁腺的有效性及对术后甲状旁腺保护和甲状旁腺激素表达的影响。方法 选择2020年1月至2022年9月在邢台医学高等专科学校第二附属医院收治的122例甲状腺癌患者,其中男性95例,女性27例;年龄39~51岁,平均年龄45.23岁;病理类型,乳头状癌105例,滤泡性腺瘤17例;单侧切除术46例,双侧切除术76例。按照随机数表法将患者分为对照组和研究组,对照组患者行常规甲状腺癌手术,研究组患者在NIRAF技术辅助下行甲状腺切除术。比较两组患者甲状旁腺识别率、围术期指标、氧化应激指标、甲状腺功能、血钙、甲状旁腺激素(PTH)、免疫功能和并发症等参数。结果 研究组患者伤口长度、术中出血量、手术时间、术后引流量和住院时间与对照组比较,差异无统计学意义(P> 0.05)。两组患者均出现感染、发音困难和声音嘶哑等并发症,但差异无统计学意义(P> 0.05),而研究组患者术后低血钙和PTH减退比例明显低于对照组(22.95%vs 44.26%,6.56%vs 19.67%。P=0.013、0.032)。术后7 d两组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显提高,两组患者CD3^(+)CD8^(+)水平明显降低,且研究组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显高于对照组(43.83±4.59 vs 40.23±3.78,1.93±0.21 vs 1.73±0.13。P=0.000、0.000),研究组患者CD3^(+)CD8^(+)水平明显低于对照组(22.61±2.31 vs 23.81±2.62。P=0.008)。术后7 d两组患者血钙和PTH明显降低,且研究组患者血钙和PTH明显高于对照组(2.26±0.18 vs 2.15±0.25,36.98±5.16 vs 27.28±4.99。P=0.000、0.000)。研究组患者甲状腺被膜解剖前检出率为56.19%,明显高于对照组的31.19%(P=0.000);研究组患者甲状旁腺总检出率和总体准确度与对照组比较,差异无统计学意义(P> 0.05)。结论 NIRAF技术辅助识别甲状旁腺组织,可有效提高甲状腺被膜解剖前检出率,减小血钙和PTH减退幅度,增强术后甲状旁腺保护,改善免疫机能,值得临床进一步研究并推广。展开更多
文摘Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% decolorization of Reactive blue 160 (RB160), Reactive black 5 (RB5) and Amido black 10B (AB10B) respectively. Further analysis using UV-vis, HPLC, and FTIR, <sup>1</sup>H NMR had shown the degradation of the dyes. Toxicity analysis of the metabolites was performed using seed germination and plant growth on two agriculturally important plants Guar (Cyamopsis tetragonoloba) and wheat (Triticum aestivum) as well as cytotoxicity analysis using the human keratinocyte cell line (HaCaT). The dye mix appeared inhibitory for seed germination (20% - 40%), whereas metabolites were non-inhibitory for germination. Treatment of HaCaT cells with of dye mix and metabolites led into 45% and ~100% of cell viability of HaCaT cells respectively. Therefore, metabolites following degradation of the dye mix were observed to be non-toxic.
文摘目的 探讨近红外自体荧光显像(NIRAF)技术辅助识别甲状旁腺的有效性及对术后甲状旁腺保护和甲状旁腺激素表达的影响。方法 选择2020年1月至2022年9月在邢台医学高等专科学校第二附属医院收治的122例甲状腺癌患者,其中男性95例,女性27例;年龄39~51岁,平均年龄45.23岁;病理类型,乳头状癌105例,滤泡性腺瘤17例;单侧切除术46例,双侧切除术76例。按照随机数表法将患者分为对照组和研究组,对照组患者行常规甲状腺癌手术,研究组患者在NIRAF技术辅助下行甲状腺切除术。比较两组患者甲状旁腺识别率、围术期指标、氧化应激指标、甲状腺功能、血钙、甲状旁腺激素(PTH)、免疫功能和并发症等参数。结果 研究组患者伤口长度、术中出血量、手术时间、术后引流量和住院时间与对照组比较,差异无统计学意义(P> 0.05)。两组患者均出现感染、发音困难和声音嘶哑等并发症,但差异无统计学意义(P> 0.05),而研究组患者术后低血钙和PTH减退比例明显低于对照组(22.95%vs 44.26%,6.56%vs 19.67%。P=0.013、0.032)。术后7 d两组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显提高,两组患者CD3^(+)CD8^(+)水平明显降低,且研究组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显高于对照组(43.83±4.59 vs 40.23±3.78,1.93±0.21 vs 1.73±0.13。P=0.000、0.000),研究组患者CD3^(+)CD8^(+)水平明显低于对照组(22.61±2.31 vs 23.81±2.62。P=0.008)。术后7 d两组患者血钙和PTH明显降低,且研究组患者血钙和PTH明显高于对照组(2.26±0.18 vs 2.15±0.25,36.98±5.16 vs 27.28±4.99。P=0.000、0.000)。研究组患者甲状腺被膜解剖前检出率为56.19%,明显高于对照组的31.19%(P=0.000);研究组患者甲状旁腺总检出率和总体准确度与对照组比较,差异无统计学意义(P> 0.05)。结论 NIRAF技术辅助识别甲状旁腺组织,可有效提高甲状腺被膜解剖前检出率,减小血钙和PTH减退幅度,增强术后甲状旁腺保护,改善免疫机能,值得临床进一步研究并推广。
