Bone biomaterials have been increasingly used to reconstruct maxillary atrophic ridges.Thus,the aim of this study was to evaluate bone reconstruction in the maxilla using a homologous cortico-cancellous FFB(lyophilize...Bone biomaterials have been increasingly used to reconstruct maxillary atrophic ridges.Thus,the aim of this study was to evaluate bone reconstruction in the maxilla using a homologous cortico-cancellous FFB(lyophilized)graft and verify its reliability.Eight individuals were included from 2014 to 2018.The first surgery was performed to install homologous bone blocks in the maxilla.The period of the second intervention varied between 5 months and 15 days to 11 months(≈7.93 months).The biopsies were taken from the central region of the matured graft during the surgery for implant placement.All patients presented clinical and radiographic conditions for the installation of dental implants.There was a 100%of survival rate.The histological assessment showed that the homologous block bone graft was an osteoconductive biomaterial,with connective tissue present,and newly formed bone juxtaposed on its surface.There were bone trabeculae with osteocytes and active osteoblasts with connective tissue in the mineralization process;the remodeling process can be found through the reverse lines.A limited focus of necrosis with fibrosis was detected,with small resorption and areas of inflammatory infiltrate,but without clinical significance.The homologous block bone graft can be considered a feasible option to substitute the autogenous bone graft(gold standard),with predictable clinical and favorable histological results.The patients had a shorter surgical period,low morbidity,and an unlimited amount of biomaterial available at an accessible cost.展开更多
Femoral veins of 17 mongrel dogs were distended with 40 kpa, 80 kpa and 120 kpa respectively prior to grafting to colateral femoral arteries. The sections were harvested at intervals of immediatness, 1 week, 4 week an...Femoral veins of 17 mongrel dogs were distended with 40 kpa, 80 kpa and 120 kpa respectively prior to grafting to colateral femoral arteries. The sections were harvested at intervals of immediatness, 1 week, 4 week and 16 week followed with scanning electron microscopy (SEM) examination. The endothelial cells of grafted veins were investigated by computerized imaging analysis system. The results showed that desquamation extent of intimal layer correlated positively with the pressure. 80 kpa and 120 kpa caused relatively severe damage to the endothelium, which was significantly different from that of control group (P<0. 05). We conclude that preimplantation distention shooed be employed with less than 80 kpa, and 120 kPa shoud be avoided due to its damage to the development of reendothelialization. Nevertheless, the distension makes little notable impact to graft Patency rate as demonstrated in this experiment.展开更多
Experiments on maxillofacial bone tissue engineering showed the promising result;however, its healing mechanisms and effectiveness had not been fully understood. The aim of this study is to compare the bone healing me...Experiments on maxillofacial bone tissue engineering showed the promising result;however, its healing mechanisms and effectiveness had not been fully understood. The aim of this study is to compare the bone healing mechanism and osteogenic capacity between bovine bone mineral loaded with hAMSC and autogenous bone graft in the reconstruction of critical size mandibular bone defect. Critical size defects were made at the mandible of 45 New Zealand white rabbits reconstructed with BBM-hAMSC, BBM alone, and ABG, respectively. At the end of first, second, and twelfth weeks, five rabbits from each experimental week were sacrificed for histology and immunohistochemistry staining. Expressions of vascular endothelial growth factor (VEGF), bone mor-phogenic proteins-2 (BMP2), Runx2 and the amount of angiogenesis were analyzed in the first and second week groups, while expressions of Runx2, osteocalcin, collagen type-I fibres, trabecular area and bone incorporation were analyzed in the twelfth week groups. The result showed that expressions of VEGF, BMP2 and Runx2 as well as the amount of angiogenesis were higher in ABG compared with BBM-hAMSC group in the first and second weeks of healing. The result of twelfth week of healing showed that expressions of Runx2 and osteocalcin as well as the thickness of collagen type-I fibres were significantly higher in BBM-hAMSC compared to ABG group, while there was no statistically difference in trabecular area and bone incorporation between BBM-hAMSC and ABG group. This study concluded that early healing activities were higher in auto-genous bone graft than in BBM-hAMSC, while osteogenic activities in the late stage of healing were higher in BBM-hAMSC compared to autogenous bone graft. It was also concluded that the osteo-genic capacity of BBM-hAMSC was comparable to autogenous bone graft in the reconstruction of critical size defect in the mandible.展开更多
Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferatio...Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells(vascular smooth muscle cells, VSMCs)were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they was more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control).TEA(blocker of Ca2+ activated potassium channels)increased MTT A490 nm value of VSMCs from femoral vein in a dose dependent manner(P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts (1-4) week after transplantation(P<0.05, n=5).Conclusion: KCa is inhibited in autogenous vein graft, which account for vasospasm and intimal proliferation.展开更多
Purpose: To investigate the periapical tissue response after root end filling with intermediate restorative material (IRM) and filling of the root-end resection bone defects with autogenous bone or a bone graft substi...Purpose: To investigate the periapical tissue response after root end filling with intermediate restorative material (IRM) and filling of the root-end resection bone defects with autogenous bone or a bone graft substitute in comparison to empty controls. Materials and Methods: Vital roots of the second, third and fourth mandibular premolars in six healthy mongrel dogs were apectomized. The root canals were prepared and sealed with IRM following a standardized surgical procedure. The resection bone defects were either filled with autogenous bone (PB) or one of the bone graft substitutes;CERAMENTTM|BONE VOID FILLER, ChronOS?, TigranTM PTG, Easygraft? CLASSIC or left empty. After 120 days the animals were sacrificed and the specimens were analyzed radiologically and histologically. Kruskal-Wallis and Mann-Whitney tests were performed for statistical evaluation. Results: 34 sections were analyzed histologically. The evaluation revealed a variation in the outcome amongst the tested options, regarding reestablishment of the periapical bone healing and inflammatory infiltration in the sections. According to the tested variables, there was no statistical significant difference between the materials when comparing all groups as a whole. When comparing individual materials to each other there was statistical differences among some of the tested materials. Conclusion: The healing outcome after periapical surgery of a five-wall resection defect could not be increased by infill with autogenous bone or bone graft substitutes. The most important factor for the healing outcome in periapical surgery is the quality of the root-end sealing. The healing outcome after some of the tested bone substitutes, might be improved by longer healing time.展开更多
Objective: To explore the alleviation of arterial intimal hyperplasia and improvement of outflow by inserting an autogenous vein cuff between poly tetraflu oroethylene (PTFE) graft and arteri al an astomosis. Methods:...Objective: To explore the alleviation of arterial intimal hyperplasia and improvement of outflow by inserting an autogenous vein cuff between poly tetraflu oroethylene (PTFE) graft and arteri al an astomosis. Methods: Twenty-four hindlimbs of 12 mongrel dogs were randomly divided into control group and experimental group. Sole PTFE bypasses were made in the control group, an autologous vein cuff was inserted in the distal anastomosis in the experimental group. Eight weeks after operation, angiography was made and specimens were harvested, histomorphological studies under microscope and picture analysis with computer were carried out, scanning electromicroscopy on the vein cuff was made. Results: Angiography demonstrated the patency of control and experimental group was 16. 7% and 66. 7%, respectively; Computer gave the intimal thickness: (483. 5 ± 67. 3) μm and (147. 4 ± 38. 6) μm, respectively; no obvious change was seen in medial thickness; area of intimal hyperplasia was (5217 ± 1 123) (pixel) and (31 17 ± 890) (pixel), respectively, accounting (80. 9 ± 17. 2)% and (47. 7 ± 13. 7)% of the sectional area of vessel lumen. The interpositional vein was arteriolization. Conclusion: The interpositional autologous vein cuff can obviously mitigate the arterial intimal hyperplasia after PTFE bypasses, improving postoperative patency of vascular surgery.展开更多
目的探究四肢长骨干骨折术后无菌性骨不连患者采用锁定钢板联合自体髂骨植骨术治疗的临床疗效。方法简单随机选取2022年1月—2023年12月山东省文登整骨烟台医院收治的80例四肢长骨干骨折术后无菌性骨不连患者作为研究对象,依据不同手术...目的探究四肢长骨干骨折术后无菌性骨不连患者采用锁定钢板联合自体髂骨植骨术治疗的临床疗效。方法简单随机选取2022年1月—2023年12月山东省文登整骨烟台医院收治的80例四肢长骨干骨折术后无菌性骨不连患者作为研究对象,依据不同手术方法分为对照组和观察组,各40例。对照组实施交锁髓内钉联合侧方钢板内固定,观察组在对照组的基础上联合自体髂骨植骨术。对比两组临床指标、骨代谢水平、并发症发生率、治疗效果、Radius联合评分系统(Radius Union Scoring System,RUSS)评分。结果观察组术中出血量、术后引流量均少于对照组,内固定时间短于对照组,差异有统计学意义(P<0.05)。观察骨代谢水平、治疗优良率、RUSS评分高于对照组,差异有统计学意义(P均<0.05)。观察组并发症总发生率为2.50%(1/40),低于对照组的20.00%(8/40),差异有统计学意义(χ^(2)=4.507,P<0.05)。结论在对四肢长骨干骨折术后无菌性骨不连患者治疗时,锁定钢板联合自体髂骨植骨术可显著改善骨代谢水平,减轻疼痛,有助于加快骨愈合,且对并发症有较好的预防作用。展开更多
文摘Bone biomaterials have been increasingly used to reconstruct maxillary atrophic ridges.Thus,the aim of this study was to evaluate bone reconstruction in the maxilla using a homologous cortico-cancellous FFB(lyophilized)graft and verify its reliability.Eight individuals were included from 2014 to 2018.The first surgery was performed to install homologous bone blocks in the maxilla.The period of the second intervention varied between 5 months and 15 days to 11 months(≈7.93 months).The biopsies were taken from the central region of the matured graft during the surgery for implant placement.All patients presented clinical and radiographic conditions for the installation of dental implants.There was a 100%of survival rate.The histological assessment showed that the homologous block bone graft was an osteoconductive biomaterial,with connective tissue present,and newly formed bone juxtaposed on its surface.There were bone trabeculae with osteocytes and active osteoblasts with connective tissue in the mineralization process;the remodeling process can be found through the reverse lines.A limited focus of necrosis with fibrosis was detected,with small resorption and areas of inflammatory infiltrate,but without clinical significance.The homologous block bone graft can be considered a feasible option to substitute the autogenous bone graft(gold standard),with predictable clinical and favorable histological results.The patients had a shorter surgical period,low morbidity,and an unlimited amount of biomaterial available at an accessible cost.
文摘Femoral veins of 17 mongrel dogs were distended with 40 kpa, 80 kpa and 120 kpa respectively prior to grafting to colateral femoral arteries. The sections were harvested at intervals of immediatness, 1 week, 4 week and 16 week followed with scanning electron microscopy (SEM) examination. The endothelial cells of grafted veins were investigated by computerized imaging analysis system. The results showed that desquamation extent of intimal layer correlated positively with the pressure. 80 kpa and 120 kpa caused relatively severe damage to the endothelium, which was significantly different from that of control group (P<0. 05). We conclude that preimplantation distention shooed be employed with less than 80 kpa, and 120 kPa shoud be avoided due to its damage to the development of reendothelialization. Nevertheless, the distension makes little notable impact to graft Patency rate as demonstrated in this experiment.
文摘Experiments on maxillofacial bone tissue engineering showed the promising result;however, its healing mechanisms and effectiveness had not been fully understood. The aim of this study is to compare the bone healing mechanism and osteogenic capacity between bovine bone mineral loaded with hAMSC and autogenous bone graft in the reconstruction of critical size mandibular bone defect. Critical size defects were made at the mandible of 45 New Zealand white rabbits reconstructed with BBM-hAMSC, BBM alone, and ABG, respectively. At the end of first, second, and twelfth weeks, five rabbits from each experimental week were sacrificed for histology and immunohistochemistry staining. Expressions of vascular endothelial growth factor (VEGF), bone mor-phogenic proteins-2 (BMP2), Runx2 and the amount of angiogenesis were analyzed in the first and second week groups, while expressions of Runx2, osteocalcin, collagen type-I fibres, trabecular area and bone incorporation were analyzed in the twelfth week groups. The result showed that expressions of VEGF, BMP2 and Runx2 as well as the amount of angiogenesis were higher in ABG compared with BBM-hAMSC group in the first and second weeks of healing. The result of twelfth week of healing showed that expressions of Runx2 and osteocalcin as well as the thickness of collagen type-I fibres were significantly higher in BBM-hAMSC compared to ABG group, while there was no statistically difference in trabecular area and bone incorporation between BBM-hAMSC and ABG group. This study concluded that early healing activities were higher in auto-genous bone graft than in BBM-hAMSC, while osteogenic activities in the late stage of healing were higher in BBM-hAMSC compared to autogenous bone graft. It was also concluded that the osteo-genic capacity of BBM-hAMSC was comparable to autogenous bone graft in the reconstruction of critical size defect in the mandible.
文摘Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells(vascular smooth muscle cells, VSMCs)were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they was more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control).TEA(blocker of Ca2+ activated potassium channels)increased MTT A490 nm value of VSMCs from femoral vein in a dose dependent manner(P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts (1-4) week after transplantation(P<0.05, n=5).Conclusion: KCa is inhibited in autogenous vein graft, which account for vasospasm and intimal proliferation.
文摘Purpose: To investigate the periapical tissue response after root end filling with intermediate restorative material (IRM) and filling of the root-end resection bone defects with autogenous bone or a bone graft substitute in comparison to empty controls. Materials and Methods: Vital roots of the second, third and fourth mandibular premolars in six healthy mongrel dogs were apectomized. The root canals were prepared and sealed with IRM following a standardized surgical procedure. The resection bone defects were either filled with autogenous bone (PB) or one of the bone graft substitutes;CERAMENTTM|BONE VOID FILLER, ChronOS?, TigranTM PTG, Easygraft? CLASSIC or left empty. After 120 days the animals were sacrificed and the specimens were analyzed radiologically and histologically. Kruskal-Wallis and Mann-Whitney tests were performed for statistical evaluation. Results: 34 sections were analyzed histologically. The evaluation revealed a variation in the outcome amongst the tested options, regarding reestablishment of the periapical bone healing and inflammatory infiltration in the sections. According to the tested variables, there was no statistical significant difference between the materials when comparing all groups as a whole. When comparing individual materials to each other there was statistical differences among some of the tested materials. Conclusion: The healing outcome after periapical surgery of a five-wall resection defect could not be increased by infill with autogenous bone or bone graft substitutes. The most important factor for the healing outcome in periapical surgery is the quality of the root-end sealing. The healing outcome after some of the tested bone substitutes, might be improved by longer healing time.
文摘Objective: To explore the alleviation of arterial intimal hyperplasia and improvement of outflow by inserting an autogenous vein cuff between poly tetraflu oroethylene (PTFE) graft and arteri al an astomosis. Methods: Twenty-four hindlimbs of 12 mongrel dogs were randomly divided into control group and experimental group. Sole PTFE bypasses were made in the control group, an autologous vein cuff was inserted in the distal anastomosis in the experimental group. Eight weeks after operation, angiography was made and specimens were harvested, histomorphological studies under microscope and picture analysis with computer were carried out, scanning electromicroscopy on the vein cuff was made. Results: Angiography demonstrated the patency of control and experimental group was 16. 7% and 66. 7%, respectively; Computer gave the intimal thickness: (483. 5 ± 67. 3) μm and (147. 4 ± 38. 6) μm, respectively; no obvious change was seen in medial thickness; area of intimal hyperplasia was (5217 ± 1 123) (pixel) and (31 17 ± 890) (pixel), respectively, accounting (80. 9 ± 17. 2)% and (47. 7 ± 13. 7)% of the sectional area of vessel lumen. The interpositional vein was arteriolization. Conclusion: The interpositional autologous vein cuff can obviously mitigate the arterial intimal hyperplasia after PTFE bypasses, improving postoperative patency of vascular surgery.
文摘目的探究四肢长骨干骨折术后无菌性骨不连患者采用锁定钢板联合自体髂骨植骨术治疗的临床疗效。方法简单随机选取2022年1月—2023年12月山东省文登整骨烟台医院收治的80例四肢长骨干骨折术后无菌性骨不连患者作为研究对象,依据不同手术方法分为对照组和观察组,各40例。对照组实施交锁髓内钉联合侧方钢板内固定,观察组在对照组的基础上联合自体髂骨植骨术。对比两组临床指标、骨代谢水平、并发症发生率、治疗效果、Radius联合评分系统(Radius Union Scoring System,RUSS)评分。结果观察组术中出血量、术后引流量均少于对照组,内固定时间短于对照组,差异有统计学意义(P<0.05)。观察骨代谢水平、治疗优良率、RUSS评分高于对照组,差异有统计学意义(P均<0.05)。观察组并发症总发生率为2.50%(1/40),低于对照组的20.00%(8/40),差异有统计学意义(χ^(2)=4.507,P<0.05)。结论在对四肢长骨干骨折术后无菌性骨不连患者治疗时,锁定钢板联合自体髂骨植骨术可显著改善骨代谢水平,减轻疼痛,有助于加快骨愈合,且对并发症有较好的预防作用。
