Efficacy of Huangma Ding or autologous platelet-rich gel for the diabetic lower extremity arterial disease patients with foot ulcers

BACKGROUND Diabetes foot is one of the most serious complications of diabetes and an important cause of death and disability,traditional treatment has poor efficacy and there is an urgent need to develop a practical treatment method.AIM To investigate whether Huangma Ding or autologous platelet-rich gel(APG)treatment would benefit diabetic lower extremity arterial disease(LEAD)patients with foot ulcers.METHODS A total of 155 diabetic LEAD patients with foot ulcers were enrolled and divided into three groups:Group A(62 patients;basal treatment),Group B(38 patients;basal treatment and APG),and Group C(55 patients;basal treatment and Huangma Ding).All patients underwent routine follow-up visits for six months.After follow-up,we calculated the changes in all variables from baseline and determined the differences between groups and the relationships between parameters.RESULTS The infection status of the three groups before treatment was the same.Procalcitonin(PCT)improved after APG and Huangma Ding treatment more than after traditional treatment and was significantly greater in Group C than in Group B.Logistic regression analysis revealed that PCT was positively correlated with total amputation,primary amputation,and minor amputation rates.The ankle-brachial pressure and the transcutaneous oxygen pressure in Groups B and C were greater than those in Group A.The major amputation rate,minor amputation rate,and total amputation times in Groups B and C were lower than those in Group A.CONCLUSION Our research indicated that diabetic foot ulcers(DFUs)lead to major amputation,minor amputation,and total amputation through local infection and poor microcirculation and macrocirculation.Huangma Ding and APG were effective attreating DFUs.The clinical efficacy of Huangma Ding was better than that of autologous platelet gel,which may be related to the better control of local infection by Huangma Ding.This finding suggested that in patients with DFUs combined with coinfection,controlling infection is as important as improving circulation.

Autologous peripheral blood-derived orthobiologics:Different types and their effectiveness in managing knee osteoarthritis

Knees are the most commonly impacted weight-bearing joints in osteoarthritis(OA),affecting millions of people worldwide.With increasing life spans and obesity rates,the incidence of knee OA will further increase,leading to a significant increase in the economic burden.Conventional treatment modalities utilized to manage knee OA have limitations.Over the last decade,the role of various autologous peripheral blood-derived orthobiologics(APBOs)for the treatment of knee OA has been extensively investigated.This editorial provided an overview and focused on defining and shedding light on the current state of evidence based on the most recent published clinical studies concerning the use of APBO for the management of knee OA.While numerous studies have demonstrated promising results for these preparations,a notable gap exists in the comparative analysis of these diverse formulations.This absence of head-to-head studies poses a considerable challenge for physicians/surgeons in determining the optimal preparation for managing knee OA and achieving sustained longterm results.Thus,more adequately powered,multicenter,prospective,doubleblind,randomized controlled trials with longer follow-ups are needed to establish the long-term efficacy and to aid physicians/surgeons in determining the optimal APBO for the management of knee OA.

Effect of Bismuth Subgallate/Borneol Combined with Autologous Platelet-Rich Gel in the Treatment of Patients with Diabetic Foot Ulcers

Objective: To explore the effect of Bismuth Subgallate/Borneol (SuileTM BSB) healing dressing combined with autologous platelet-rich gel (APG) in the treatment of diabetic foot ulcer (DFU). Methods: A total of 120 patients with DFU hospitalized in the Changsha Central Hospital from August 2020 to September 2021 were selected and randomly divided into an experimental group (BSB + APG, n = 60) and a control group (BSB, n = 60) according to random number table method. The total therapeutic effect, healing time, hospital stay, level indexes of various inflammatory factors before and after treatment and ulcer area were observed in the two groups. Results: The total effect of the control group was worse than that of the experimental group, and the data between the two groups were significant (P 0.05);after treatment, the levels of inflammatory factors including WBC, CRP, IL-6 and TNF-α in the control group were higher than those in the experimental group, and there was significant difference between the two groups (P 0.05);after 14 days of treatment, the ulcer area in the control group was larger than that in the experimental group, and the data between the two groups were significant (P Conclusion: BSB combined with APG can achieve better therapeutic effect, reduce the inflammatory reaction of patients, and promote wound healing in the treatment of patients with diabetic foot ulcer.

Effect of Xuesaitong Combined with Autologous Platelet-rich Gel on Blood Glucose,AT-III,TNF-αand 24-h Urinary Protein Level in Patients with Diabetic Foot

[Objectives]To investigate the clinical effect of different doses of Xuesaitong combined with autologous platelet-rich gel(APG)on patients with diabetic foot(DF).[Methods]90 patients with diabetic foot admitted to our hospital from February 2017 to February 2019 were enrolled in the study.According to the random number table method,the subjects were divided into study group A and B and control group C.Group A was given a low dose of Xuesaitong combined with APG,while group B was given high-dose Xuesaitong combined with APG and group C was treated only with APG.Patients in the three groups were observed and the changes of related indexes were detected.[Results]After treatment,with regard to the three groups,the fasting blood glucose,2-h postprandial blood glucose level,HbA1c,TNF-α,Hcy,blood urea nitrogen(BUN),creatinine(Cr)and 24-h urine protein levels were all decreased,yet AT-III level was increased,and granulation tissue coverage and thickness,wound clearance rate were increased,while the repair time of ulcer surface was significantly reduced.The above indexes were all significantly different(P<0.05).Compared with the control group C,the changes of the indexes in the study group A and B were the same as above,and the difference was significant(P<0.05).Compared with the study group A,the level of AT-III in group B was significantly increased(P<0.05),while TNF-α,Hcy,BUN,Cr and 24-h urine protein levels were significantly decreased(P<0.05).There was no significant difference in the other indexes between groups A and B(P>0.05).[Conclusions]Xuesaitong combined with APG could effectively reduce the blood sugar level of DF patients,improve the clinical indexes,promote wound healing,and the high-dose group had more significant advantages and was worthy of promotion.

Clinical and Magnetic Resonance Image Changes of Platelet-Rich Plasma Therapy in Combination with Human Mesenchymal Stem Cells from Autologous Adipose Tissue for Knee Osteoarthritis Treatment

Objective: To evaluate the efficacy based on clinical symptom and on magnetic resonance image of platelet-rich plasma therapy in combination with mesenchymal stem cells from autologous adipose tissue for knee osteoarthritis treatment. Patients and Method: 30 patients including 26 females and 4 males;correspondingly, 60 knee joints were diagnosed with osteoarthritis with stages II - III of Kellgren and Lawrence, their mean age was 58.63 ± 11.11. All were injected with autologous platelet-rich plasma that was extracted by PRP set, APC 30 PRP PRCEDURE PRAK and autologously extracted mesenchymal stem cells from abdominal adipose tissue using the ADI-25-01 ADIPOSEPRCEDURE PRAK of USA. Results: After 12 months: the pain level according to VAS score at the right knee joint was decreased from 6.0 ± 1.28 before treatment to 1.9 ± 0.3;VAS score at the left knee joint was decreased from 6.43 ± 1.19 to 2.25 ± 0.43. Total Lequene score at right knee joint was decreased from 16.04 ± 1.57 before treatment to 4.31 ± 1.04, at left knee joint was decreased from 17.52 ± 1.74 before treatment to 5.15 ± 1.48. Total WOMAC score at right knee joint was decreased from 55.93 ± 5.56 to 10.37 ± 1.56;at left knee joint was decreased from 53.97 ± 5.57 to 10.07 ± 1.59. There were 86.77% joints with cartilage thickness change and the patellar cartilage thickness was increased from 1.56 ± 0.09 mm before treatment to 1.65 ± 0.09 mm. Conclusion: The treatment of knee osteoarthritis by platelet-rich plasma therapyin combination with mesenchymal stem cells from autologous adipose tissue is effective in reducing pain, improving patient's mobility and walking function, reforming articular cartilage thickness on magnetic resonance image.

Therapeutic Effectiveness of Leukocyte-and Platelet-rich Fibrin for Diabetic Foot Ulcers:A Retrospective Study

Objective Diabetic foot ulcer(DFU)is one of the most serious complications of diabetes.Leukocyte-and platelet-rich fibrin(L-PRF)is a second-generation autologous platelet-rich plasma.This study aims to investigate the clinical effects of L-PRF in patients with diabetes in real clinical practice.Methods Patients with DFU who received L-PRF treatment and standard of care(SOC)from 2018 to 2019 in Tongji Hospital were enrolled.The clinical information including patient characteristics,wound evaluation(area,severity,infection,blood supply),SOC of DFU,and images of ulcers was retrospectively extracted and analyzed.L-PRF treatment was performed every 7±2 days until the ulcer exhibited complete epithelialization or an overall percent volume reduction(PVR)greater than 80%.Therapeutic effectiveness,including overall PVR and the overall and weekly healing rates,was evaluated.Results Totally,26 patients with DFU were enrolled,and they had an ulcer duration of 47.0(35.0,72.3)days.The severity and infection of ulcers varied,as indicated by the Site,Ischemia,Neuropathy,Bacterial Infection,and Depth(SINBAD)scores of 2–6,Wagner grades of 1–4,and the Perfusion,Extent,Depth,Infection and Sensation(PEDIS)scores of 2–4.The initial ulcer volume before L-PRF treatment was 4.94(1.50,13.83)cm3,and the final ulcer volume was 0.35(0.03,1.76)cm3.The median number of L-PRF doses was 3(2,5).A total of 11 patients achieved complete epithelialization after the fifth week of treatment,and 19 patients achieved at least an 80%volume reduction after the seventh week.The overall wound-healing rate was 1.47(0.63,3.29)cm3/week,and the healing rate was faster in the first 2 weeks than in the remaining weeks.Concurrent treatment did not change the percentage of complete epithelialization or healing rate.Conclusion Adding L-PRF to SOC significantly improved wound healing in patients with DFU independent of the ankle brachial index,SINBAD score,or Wagner grade,indicating that this method is appropriate for DFU treatment under different clinical conditions.

Methacrylated gelatin and platelet-rich plasma based hydrogels promote regeneration of critical-sized bone defects

Physiological repair of large-sized bone defects requires instructive scaffolds with appropriate mechanical properties,biocompatibility,biodegradability,vasculogenic ability and osteo-inductivity.The objective of this study was to fabricate in situ injectable hydrogels using platelet-rich plasma(PRP)-loaded gelatin methacrylate(GM)and employ them for the regeneration of large-sized bone defects.We performed various biological assays as well as assessed the mechanical properties of GM@PRP hydrogels alongside evaluating the release kinetics of growth factors(GFs)from hydrogels.The GM@PRP hydrogels manifested sufficient mechanical properties to support the filling of the tissue defects.For biofunction assay,the GM@PRP hydrogels significantly improved cell migration and angiogenesis.Especially,transcriptome RNA sequencing of human umbilical vein endothelial cells and bone marrow-derived stem cells were performed to delineate vascularization and biomineralization abilities of GM@PRP hydrogels.The GM@PRP hydrogels were subcutaneously implanted in rats for up to 4 weeks for preliminary biocompatibility followed by their transplantation into a tibial defect model for up to 8 weeks in rats.Tibial defects treated with GM@PRP hydrogels manifested significant bone regeneration as well as angiogenesis,biomineralization,and collagen deposition.Based on the biocompatibility and biological function of GM@PRP hydrogels,a new strategy is provided for the regenerative repair of large-size bone defects.

兔自体细胞-载体复合物修复关节软骨缺损的实验研究

目的研究兔MSCs向软骨细胞方向诱导后复合自体双相骨基质明胶(bone matrix gelatin,BMG)对膝关节软骨缺损的修复作用。方法健康成年新西兰大白兔24只,雌雄不限,体重2～3kg,随机分为A、B、C组,每组8只。取A组骨髓行原代培养,胰酶消化按1∶2比例传至第5代,倒置相差显微镜观察细胞形态。取1、3、5代细胞绘制生长曲线。于第3代MSCs生长密集时加入TGF-β110ng/mL、IGF-110ng/mL、维生素C50ng/mL诱导8d后倒置相差显微镜下观察,爬片行免疫组织化学及Mallory染色。取A组髂骨按Urist方法制备双相BMG,将第3代经诱导8d得到的种子细胞以5×106/mL密度接种于自体BMG制备细胞-载体复合物,体外培养3d后扫描电镜观察。制作膝关节软骨直径3mm,深3mm的缺损模型,A组植入自体细胞-载体复合物,B组植入自体BMG,C组不作处理,第8、12周取材行大体观察、HE染色、免疫组织化学染色观察,Wakitani法组织学评分。结果倒置相差显微镜观察MSCs原代细胞呈短梭形;传代细胞呈长梭形。传代细胞1～3d增殖缓慢,3d后增殖旺盛,7～8d进入平台期,第3代增殖最为旺盛。诱导后MSCs细胞呈椭圆形,Ⅱ型胶原、S-100免疫组织化学染色阳性,Mallory染色胞浆蓝染。细胞-载体复合物扫描电镜观察:BMG结构符合细胞载体要求,细胞种植于BMG后生长良好。动物实验:大体观察A组术后8、12周缺损处均由透明软骨样组织修复,8周较12周表面稍凹陷;B、C组术后8、12周均为纤维样组织修复,表面凹凸不平。组织学观察HE染色A组术后8、12周修复组织与周围软骨组织结合良好,以圆形、椭圆形透明软骨样细胞为主,12周较8周细胞数多且大;B、C组均为纤维样组织修复,12周较8周纤维样组织增厚。Ⅱ型胶原免疫组织化学染色A组术后8周呈阳性、12周呈强阳性;B、C组均无表达。Wakitani评分术后8周A、B、C组分别为(3.50±1.51)、(10.00±1.41)、(12.00±0.93)分,术后12周分别为(1.13±0.99)、(8.38±1.30)、(10.13±1.64)分,A组优于B、C组,B组优于C组,差异均有统计学意义(P<0.05)。结论自体MSCs向软骨细胞方向诱导后,复合自体BMG对关节软骨缺损有较好的修复作用。

异体骨基质明胶颗粒骨水泥复合牛骨形态发生蛋白修复兔挠骨缺损

探讨异体骨基质明胶颗粒、骨水泥作为bBMP载体及释放系统修复兔烧骨缺损的成骨方式及诱导成骨能力．方法：复合材料及自体骨分别移植于成年新西兰兔烧骨基准大小骨缺损，术后不同时期进行X线、组织形态学、ECT，扫描电镜观察，比较复合材料修复骨缺损的方式及能力．结果：复合材料修复成年新西兰兔挠骨基准大小骨缺损，与新鲜自体骨移植相比，修复程度及愈合率无显著差异；复合材料和新鲜自体骨移植的愈合过程及成骨方式相同．结论：异体骨基质明胶颗粒、骨水泥是bBMP的良好载体，两者复合后有良好诱导成骨能力，用于修复兔挠骨缺损，效果良好．

重组人骨形态发生蛋白2对壳聚糖-明胶多孔复合支架理化性能的影响

目的以组织工程学技术为基础,制备壳聚糖-明胶/骨形态发生蛋白2(BMP-2)多孔复合支架,讨论壳聚糖与明胶的不同配比及向复合材料中加入BMP-2对支架的微观结构和理化性能的影响。方法分别用不同配比的壳聚糖和明胶制备复合支架,并向1组配比中加入BMP-2。以扫描电镜观察支架的表面特征,测定支架的孔径值、材料的吸水率及表观密度。结果壳聚糖-明胶多孔复合支架比表面积大,内孔结构多形性高、且大量连通;壳聚糖与明胶配比为4︰1时的复合支架孔径最小,孔壁最厚;配比为1︰4时孔径最大,孔壁最薄;壳聚糖与明胶配比为1︰1时吸水率及表观密度达到最大,分别为(318±75)%和(0.085±0.013)g/cm3;向复合支架中加入BMP-2对支架的微观结构、吸水率及表观密度无明显影响。结论应用冷冻干燥法可以成功制备壳聚糖-明胶多孔复合支架,支架的微观结构与理化性能与壳聚糖与明胶的配比密切相关,向支架中加入BMP-2对材料的理化性能无明显影响。

自体富血小板凝胶治疗难治性糖尿病皮肤溃疡的疗效及对血清炎症因子的影响

目的分析自体富血小板凝胶治疗难治性糖尿病皮肤溃疡的临床疗效及对血清炎症因子的影响。方法选择糖尿病皮肤溃疡患者160例,随机分成对照组和研究组各80例。对照组患者接受常规碘伏消毒治疗,研究组在常规治疗基础上给予自体富血小板凝胶进行治疗。比较2组患者治疗前后炎症因子、溃疡面积及临床疗效。结果治疗后,2组患者的TNF-α、IL-6水平均显著低于治疗前,且研究组显著低于对照组(P<0.05);治疗后,2组患者溃疡面积均显著小于治疗前,且研究组显著小于对照组(P<0.05);研究组治疗总有效率为93.8%,显著高于对照组的65.0%(P<0.05)。结论自体富血小板凝胶能够有效降低TNF-α、IL-6等炎性因子水平,减小糖尿病皮肤溃疡面积,提高患者生活质量。

Strategies and challenges in the treatment of chronic venous leg ulcers

Evaluating patients with chronic venous leg ulcers(CVLUs)is essential to find the underlying etiology.The basic tenets in managing CVLUs are to remove the etiological causes,to address systemic and metabolic conditions,to examine the ulcers and artery pulses,and to control wound infection with debridement and eliminating excessive pressure on the wound.The first-line treatments of CVLUs remain wound care,debridement,bed rest with leg elevation,and compression.Evidence to support the efficacy of silver-based dressings in healing CVLUs is unavailable.Hydrogen peroxide is harmful to the growth of granulation tissue in the wound.Surgery options include a high ligation with or without stripping or ablation of the GSVs depending on venous reflux or insufficiency.Yet,not all CVLUs are candidates for surgical treatment because of comorbidities.When standard care of wound for 4 wk failed to heal CVLUs effectively,use of advanced wound care should be considered based on the available evidence.Negative pressure wound therapy facilitates granulation tissue development,thereby helping closure of CVLUs.Autologous split-thickness skin grafting is still the gold standard approach to close huge CVLUs.Hair punch graft appears to have a better result than traditional hairless punch graft for CVLUs.Application of adipose tissue or placenta-derived mesenchymal stem cells is a promising therapy for wound healing.Autologous platelet-rich plasma provides an alternative strategy for surgery for safe and natural healing of the ulcer.The confirmative efficacy of current advanced ulcer therapies needs more robust evidence.

自体外周血干细胞与骨基质明胶复合移植治疗骨缺损

目的观察自体外周血干细胞(autologous peripheral blood stem cells,APBSC)/表面脱钙骨基质明胶(bone matrix gelatin,BMG)共移植修复节段性骨缺损的疗效。方法 36例患者随机分为APBSC/BMG组(A组)及BMG组(B组)各18例,均行手术治疗,并行牢固内外固定。结果术后平均愈合时间A组为5个月,B组为8个月,两组间差异具有统计学意义(P<0.05);治疗期间患者未见明显不良反应。结论 APBSC/BMG复合移植较单纯BMG移植治疗骨缺损疗效好,无不良反应。

耳显微镜下明胶海绵吸附自体血贴补修复外伤性鼓膜穿孔162例体会

目的:探究耳显微镜下明胶海绵吸附自体血贴补修复外伤性鼓膜穿孔的效果。方法:选择2012年1月-2017年12月笔者所在医院收治的162例外伤性鼓膜穿孔患者为研究对象,所有患者均给予明胶海绵吸附自体血贴补修复治疗,观察患者鼓膜穿孔修复的手术效果及语言频率(0.5、1、2 kHz)平均气骨导差值是否提高。结果:162例患者中治愈48例,好转90例,无效24例,总好转率为85.2%。患者治疗后的平均气骨导差值显著高于治疗前,差异有统计学意义(P<0.05)。其中3例患者出现排斥反应,半年后采用取乳突部骨膜行鼓膜修补术后治愈。2例穿孔鼓膜术后部分愈合,患者不愿再手术,半年后失访。其余穿孔者全部愈合。结论:耳显微镜下明胶海绵吸附自体血贴补修复外伤性鼓膜穿孔效果显著,排斥反应少,值得临床推荐。

四肢骨折术中骨折小碎片的处理经验

目的探讨四肢骨折术中骨折小碎片处理的方法及意义。方法四肢粉碎性骨折患者713例,其中男性505例,女性208例;年龄2～96岁,平均年龄33岁。应用明胶海绵、可吸收线在手术中固定骨折小碎片进行治疗。结果 675例用此方法处理治疗的患者,术后配合积极的康复治疗,随访1～12个月,患者骨折小碎片复位好,至骨折愈合未发生移位,1年后骨折愈合;35例开放性骨折患者术后1年断端骨痂部分生长,延迟愈合,经继续康复治疗6个月至1年后骨折愈合;3例骨折断端无骨痂生长,骨折断端硬化,骨不连,再次手术后12~20个月后骨折愈合。结论利用明胶海绵、可吸收线固定处理骨折小碎片,取得良好的临床效果,具有处理简单、术后组织反应小、组织相容性好、不需再次手术取出内固定物、疗效肯定等优点。

自体血浆替换联合琥珀酰明胶在体外循环预充中的应用效果

目的对自体血浆替换联合琥珀酰明胶在体外循环中的应用效果进行探析。方法选取2017年6月-2018年6月来该院进行心脏瓣膜置换手术的病患共100例作为实验研究对象,根据随机数字法分为对照组和观察组各50例,对照组病患体外循环预充液运用琥珀酰明胶,观察组病患体外循环运用自体血浆替换琥珀酰明胶预充液。对比两组病患体外循环预充前,中,后3个阶段和手术后1 h,3 h后的血细胞比容(血细胞在全血中所占的容积百分比,即Hct),和血浆乳酸水平(Lac)。对比两组病患心脏瓣膜置换术前,术后24 h以及术中开放主动脉后1h的肌酸激酶同工酶,血清肌钙蛋白以及肌酸激酶的指标水平。结果观察组患者手术后3 h的血细胞比容(35.53±3.67)%显著高于对照组(31.23±2.96)%,差异有统计学意义(t=0.425,P<0.05);血浆乳酸水平(3.12±0.75)mg/dL显著低于对照组(5.22±1.34)mg/dL差异有统计学意义(t=0.482,P<0.05)。观察组患者术后24 h(63.39±22.08)U/L的肌酸激酶同工酶,血清肌钙蛋白(0.503±0.221)μg/L以及肌酸激酶(629.70±182.31)U/L的指标水平显著低于对照组(93.32±32.08)U/L;(0.881±0.221)μg/L;(869.95±192.52)U/L,差异有统计学意义(t=0.824,P<0.05)。结论在心脏瓣膜手术前进行体外循环预充中用自体血浆替代联合琥珀酰明胶可有效保护病患的心肌功能,增加机体有效循环血容量,具有一定价值,值得在临床推广应用。

Clinical Application of Platelet-Rich Fibrin in Dermatology

Platelet-rich fibrin(PRF)is a second-generation platelet concentrate obtained from autologous blood.PRF is composed of abundant platelets,leucocytes,and a high concentration of various growth factors and fibrinogen.The composition and three-dimensional structure of PRF enable it to effectively make cells migrate and proliferate,playing an important role in tissue repair.Furthermore,the easy preparation and low cost of PRF make it a good treatment option.Numerous articles have been published about the application of PRF in clinical practice,however,the application of PRF in dermatology has not been comprehensively reviewed.The objective of this review article was to discuss various applications of PRF in dermatology,including healing chronic wounds,treating androgenic alopecia,skin rejuvenation,autologous fat transplantation,and treating vitiligo.PRF is a promising dermatologic treatment,but lacks a standardized protocol regarding its methods of attainment and use,which needs more investigations.

犬局部脑缺血模型中不同栓子效果对比

目的制作自体血栓、明胶海绵两种不同材料及不同大小的栓子，以血管介入技术栓塞犬大脑中动脉方法观察犬局部脑栓塞模型效果。方法选取30条中华田园犬，随机分成自体血栓组、明胶海绵A组及明胶海绵B组，分别以直径1．1～1．3mm自体血栓条、直径3．0mm明胶海绵颗粒、直径1．0—1．5mm明胶海绵微粒作为栓子自导管注入颈内动脉，随后行数字减影血管造影、核磁共振、神经功能评分来评价效果。结果对比大脑中动脉闭塞成功率（明胶海绵A组90％、B组20％、自体血栓组70％），明胶海绵A组、自体血栓组与B组比较差异均有统计学意义（P〈0．05），而明胶海绵A组与自体血栓组比较差异无统计学意义（P〉0．05）；明胶海绵A组头颅核磁阳性率为80％、B组为30％、自体血栓组为50％，明胶海绵A组与B组比较差异有统计学意义（P〈0．05），而自体血栓组与A组、B组比较差异均无统计学意义（P〉0．05）；明胶海绵A组及自体血栓组神经功能评分均多位于6—7分值段，而B组多位于10～11分值段，明胶海绵A组、自体血栓组与明胶海绵B组比较差异均有统计学意义（P〈0．05），明胶海绵A组与自体血栓组比较差异无统计学意义（P〉0．05）。结论使用明胶海绵A组建立犬局部脑缺血模型较自体血栓而言具有制作简便、闭塞准确率高、模型稳定的特点。明胶海绵微粒B组在成功率、效果及模型后续观察上均不及前两者。