Humification plays a significant role in converting phenolic pollutants and forming heterogeneous polymers,but few studies have been performed to investigate exolaccase-started humification(ESH).Herein,the influences ...Humification plays a significant role in converting phenolic pollutants and forming heterogeneous polymers,but few studies have been performed to investigate exolaccase-started humification(ESH).Herein,the influences of lignin precursors(LPs)on exolaccase-induced bisphenol A(BPA)removal and humification were explored.In particular,the architectural features and botanical effects of the formed humification products were also tested.ESH was extremely beneficial in boosting BPA removal in the presence of LPs.Compared with LP-free(58.49%),100%of BPA was eliminated after the reaction with ESH for 72 h.Such a process was controlled by an exolaccasecaused random assembly of radicals,which generated a large number of hydrophobic polymers through nonspecific covalent binding of C–C and/or C–O.These humified polymers were extremely stable at pH 2.0–10.0 and20℃ to 80℃ and displayed unique functions,i.e.,scavenged 2,2-diphenyl-1-picrylhydrazyl/2,20-azinobis3-ethylbenzothiazoline-6-sulphonic acid radicals and exerted antioxidant capacities.More importantly,the functional polymers could act as auxin analogs to increase the germination index(>100%),plant biomass,and salt tolerance of radish seedlings.Our findings disclosed that ESH could not only be optimized to mitigate the ecological risks of phenolic pollutants and sequester organic carbon in environmental bioremediation,but the resulting abundant auxin analogs also contributed to agricultural productivity.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(42277019 and 42207470)the Natural Science Foundation of Anhui Province,China(2208085QD116)the Natural Science Research Project of Education Department of Anhui Province,China(KJ2021A0136 and KJ2021A0083).
文摘Humification plays a significant role in converting phenolic pollutants and forming heterogeneous polymers,but few studies have been performed to investigate exolaccase-started humification(ESH).Herein,the influences of lignin precursors(LPs)on exolaccase-induced bisphenol A(BPA)removal and humification were explored.In particular,the architectural features and botanical effects of the formed humification products were also tested.ESH was extremely beneficial in boosting BPA removal in the presence of LPs.Compared with LP-free(58.49%),100%of BPA was eliminated after the reaction with ESH for 72 h.Such a process was controlled by an exolaccasecaused random assembly of radicals,which generated a large number of hydrophobic polymers through nonspecific covalent binding of C–C and/or C–O.These humified polymers were extremely stable at pH 2.0–10.0 and20℃ to 80℃ and displayed unique functions,i.e.,scavenged 2,2-diphenyl-1-picrylhydrazyl/2,20-azinobis3-ethylbenzothiazoline-6-sulphonic acid radicals and exerted antioxidant capacities.More importantly,the functional polymers could act as auxin analogs to increase the germination index(>100%),plant biomass,and salt tolerance of radish seedlings.Our findings disclosed that ESH could not only be optimized to mitigate the ecological risks of phenolic pollutants and sequester organic carbon in environmental bioremediation,but the resulting abundant auxin analogs also contributed to agricultural productivity.
