Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the cur...Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the curative effects of Lannea acida on imidacloprid (IMI)-induced hypofertility in male Wistar rats. Methods: Rats of 150 – 200 g were administered IMI (22.5 mg/kg) for two weeks and partitioned into control (distilled water, vitamin E, clomiphene citrate) or test (aqueous (340 mg/kg), methanol (170 mg/kg) extract) groups for eight weeks treatment. Animals were sacrificed at the end of the treatment and samples were collected for sperm, antioxidant and hormonal analysis. Fertility tests were performed from treatment day 47 for fertility indices estimation. Results were expressed as mean ± SEM and one way ANOVA was applied using STATISTICA Software. Results: Exposition to IMI resulted in a significant decrease in sperm count, motility, viability and normality, testosterone and LH, coupled to an increase in oxidative stress markers. Moreover, IMI impaired male fertility evidenced by a significant drop in fertility index and litter size. Similar to clomiphene citrate and vitamin E, plant extracts significantly improved the sperm parameters, sexual hormones and decreased the oxidative stress markers. More importantly, the fertility index and litter size were restored, especially with the aqueous extract. Conclusion: Present results indicate that L. acida possesses curative potentials against IMI-induced hypofertility through its androgenic and antioxidant properties. However, the effects the extract on spermatozoa DNA structure and the fertility of offsprings from exposed parents are yet to be studied to conclude on total recovery from IMI toxicity.展开更多
本文用动态法测定averm ectin在甲醇、乙醇、正丙醇、正丁醇和正戊醇中的溶解度曲线,并分别用理想溶液模型、A pe l-b lat溶解度模型和多项式经验方程对实验测定溶解度数据进行关联。结果显示理想溶液模型的误差较大,多项式经验方程的...本文用动态法测定averm ectin在甲醇、乙醇、正丙醇、正丁醇和正戊醇中的溶解度曲线,并分别用理想溶液模型、A pe l-b lat溶解度模型和多项式经验方程对实验测定溶解度数据进行关联。结果显示理想溶液模型的误差较大,多项式经验方程的误差最小。实验得到的溶解度曲线及关联结果对averm ectin结晶工艺的研究具有较大的指导意义。展开更多
[Objective] The aim was to seek the appropriate utilization methods for imidacloprid pesticide in the production of Chinese wolfberry,as well as to provide scientific basis for the rational use of imidacloprid pestici...[Objective] The aim was to seek the appropriate utilization methods for imidacloprid pesticide in the production of Chinese wolfberry,as well as to provide scientific basis for the rational use of imidacloprid pesticide. [Method] The effect of different concentrations and spraying times of 10% WP of imidacloprid on the main secondary metabolites such as carotenoid and flavone,as well as the polysaccharide and total sugar content was studied in field. [Result] The effect of different concentrations of imidacloprid on the secondary metabolites and the content of polysaccharide of Chinese wolfberry was significant. After imidacloprid spraying,the contents of flavone,polysaccharide and total sugar on the 1st,7th and 14th d showed increasing trend,while the content of carotenoid showed decreasing trend. With the dilution times increasing,the content of the flavone,polysaccharide and total sugar showed increasing trend,while the content of carotenoid decreased,in which the highest content of the secondary metabolites and polysaccharide appeared in 1 800 times. [Conclusion] The dilution ratio of 1 800 times should be selected as the appropriate dosage of 10% WP of imidacloprid.展开更多
[Objective] To compare the translocation and distribution of imidacloprid in tobacco with spray and root irrigation application methods. [Methods] Pot experiment in the greenhouse was carried out, and LC-MSMS was used...[Objective] To compare the translocation and distribution of imidacloprid in tobacco with spray and root irrigation application methods. [Methods] Pot experiment in the greenhouse was carried out, and LC-MSMS was used to determine the con- tent of imidacloprid in different parts of tobacco plants （roots, stems, the upper, middle and lower leaves） at different time. [Results] The imidacloprid could be absorbed by root and could be transported to all parts of the tobacco plant after irrigating root, but the original deposition amount was larger and the transport efficiency was lower after spraying. [Conclusion] The translocation and distribution of imidacloprid by spraying was more uniform and the holding efficiency was better, but imidacloprid with root irrigation could act on leaf directly, and had better readily availability.展开更多
文摘Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the curative effects of Lannea acida on imidacloprid (IMI)-induced hypofertility in male Wistar rats. Methods: Rats of 150 – 200 g were administered IMI (22.5 mg/kg) for two weeks and partitioned into control (distilled water, vitamin E, clomiphene citrate) or test (aqueous (340 mg/kg), methanol (170 mg/kg) extract) groups for eight weeks treatment. Animals were sacrificed at the end of the treatment and samples were collected for sperm, antioxidant and hormonal analysis. Fertility tests were performed from treatment day 47 for fertility indices estimation. Results were expressed as mean ± SEM and one way ANOVA was applied using STATISTICA Software. Results: Exposition to IMI resulted in a significant decrease in sperm count, motility, viability and normality, testosterone and LH, coupled to an increase in oxidative stress markers. Moreover, IMI impaired male fertility evidenced by a significant drop in fertility index and litter size. Similar to clomiphene citrate and vitamin E, plant extracts significantly improved the sperm parameters, sexual hormones and decreased the oxidative stress markers. More importantly, the fertility index and litter size were restored, especially with the aqueous extract. Conclusion: Present results indicate that L. acida possesses curative potentials against IMI-induced hypofertility through its androgenic and antioxidant properties. However, the effects the extract on spermatozoa DNA structure and the fertility of offsprings from exposed parents are yet to be studied to conclude on total recovery from IMI toxicity.
基金Supported by National Natural Science Foundation of China (30860227)Ningxia Natural Science Foundation(NZ0603,NZ0639)~~
文摘[Objective] The aim was to seek the appropriate utilization methods for imidacloprid pesticide in the production of Chinese wolfberry,as well as to provide scientific basis for the rational use of imidacloprid pesticide. [Method] The effect of different concentrations and spraying times of 10% WP of imidacloprid on the main secondary metabolites such as carotenoid and flavone,as well as the polysaccharide and total sugar content was studied in field. [Result] The effect of different concentrations of imidacloprid on the secondary metabolites and the content of polysaccharide of Chinese wolfberry was significant. After imidacloprid spraying,the contents of flavone,polysaccharide and total sugar on the 1st,7th and 14th d showed increasing trend,while the content of carotenoid showed decreasing trend. With the dilution times increasing,the content of the flavone,polysaccharide and total sugar showed increasing trend,while the content of carotenoid decreased,in which the highest content of the secondary metabolites and polysaccharide appeared in 1 800 times. [Conclusion] The dilution ratio of 1 800 times should be selected as the appropriate dosage of 10% WP of imidacloprid.
基金Supported by the Key Science and Technology Project of Jinlin Tobacco Industrial Co.,Ltd.(JS-2015-07)the Scientific and Technological Innovation Project of Chinese Academy of Agricultural Sciences(ASTIP-TRIC06)~~
文摘[Objective] To compare the translocation and distribution of imidacloprid in tobacco with spray and root irrigation application methods. [Methods] Pot experiment in the greenhouse was carried out, and LC-MSMS was used to determine the con- tent of imidacloprid in different parts of tobacco plants （roots, stems, the upper, middle and lower leaves） at different time. [Results] The imidacloprid could be absorbed by root and could be transported to all parts of the tobacco plant after irrigating root, but the original deposition amount was larger and the transport efficiency was lower after spraying. [Conclusion] The translocation and distribution of imidacloprid by spraying was more uniform and the holding efficiency was better, but imidacloprid with root irrigation could act on leaf directly, and had better readily availability.
