Objective To investigate the prevalence and levels of anti-HEV IgG in the population of Jiangsu Province.Methods Total of 2 656 samples from Qindong and 11 463 samples from Anfeng were colleted.The antiHEV antibody wa...Objective To investigate the prevalence and levels of anti-HEV IgG in the population of Jiangsu Province.Methods Total of 2 656 samples from Qindong and 11 463 samples from Anfeng were colleted.The antiHEV antibody was qualitatively and quantitatively detected using ELISA kits and the references had been established.Results The positive rates of anti-HEV IgG in male and female were 55.6%and 40.1%,respectively.The positive rate of anti-HEV IgM in male and female were both 3.4%.In opposite to anti-HEV IgG,the positive rate of anti-HEV IgM in Anfeng was significant higher than that in Qindong.The mean anti-HEV IgG titers for6 age groups were 0.94,0.92,1.07,1.46,1.27,1.19 and 0.68,1.31,1.08,1.14,1.31,1.68 IU/ml,in Qindong and Anfeng region,respectively.The positive rate of anti-HEV IgG tended to increase with age and the titer of antiHEV IgG was associated with age(R>0.90).Conclusions The results in this study showed that HEV was widely prevalent in both Qindong and Anfeng of Jiansu Province and the prevalence and the anti-HEV IgG titer were associated with gender and age.展开更多
Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farm...Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood.Here,cross-sectional serological and genotypic surveys of HEV among the 1232 general population,273 workers occupationally exposed to swine,and 276 pigs in a high-density pig-breeding area,were undertaken by ELISA and nested RT-PCR methods.Anti-HEV IgG was detected in 26.22%of general population and 48.35%of occupational workers.The prevalence of swine serum HEV-Ag was 6.52%.The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population.An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age.Among the occupationally exposed group,the prevalence of anti-HEV IgG antibodies increased with age and working years.Among the 30 HEV-IgM-positive people,the infection rates of clerks in the public,peasants,pork retailers,and pig farmers were higher than those of others.A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d,and four people and four pigs shared 97.04%-100%sequence homology.This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City,and supports the notion that swine are a source of human HEV infection.展开更多
文摘Objective To investigate the prevalence and levels of anti-HEV IgG in the population of Jiangsu Province.Methods Total of 2 656 samples from Qindong and 11 463 samples from Anfeng were colleted.The antiHEV antibody was qualitatively and quantitatively detected using ELISA kits and the references had been established.Results The positive rates of anti-HEV IgG in male and female were 55.6%and 40.1%,respectively.The positive rate of anti-HEV IgM in male and female were both 3.4%.In opposite to anti-HEV IgG,the positive rate of anti-HEV IgM in Anfeng was significant higher than that in Qindong.The mean anti-HEV IgG titers for6 age groups were 0.94,0.92,1.07,1.46,1.27,1.19 and 0.68,1.31,1.08,1.14,1.31,1.68 IU/ml,in Qindong and Anfeng region,respectively.The positive rate of anti-HEV IgG tended to increase with age and the titer of antiHEV IgG was associated with age(R>0.90).Conclusions The results in this study showed that HEV was widely prevalent in both Qindong and Anfeng of Jiansu Province and the prevalence and the anti-HEV IgG titer were associated with gender and age.
基金partly supported by General Projects of Health and Family Planning Commission of Hubei Province of China no. WJ2017M174, and WJ2017M240Occupational Hazard and Identification Control of Hubei Provincial Key Laboratory Open Fund, no. OCHI2017G02
文摘Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood.Here,cross-sectional serological and genotypic surveys of HEV among the 1232 general population,273 workers occupationally exposed to swine,and 276 pigs in a high-density pig-breeding area,were undertaken by ELISA and nested RT-PCR methods.Anti-HEV IgG was detected in 26.22%of general population and 48.35%of occupational workers.The prevalence of swine serum HEV-Ag was 6.52%.The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population.An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age.Among the occupationally exposed group,the prevalence of anti-HEV IgG antibodies increased with age and working years.Among the 30 HEV-IgM-positive people,the infection rates of clerks in the public,peasants,pork retailers,and pig farmers were higher than those of others.A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d,and four people and four pigs shared 97.04%-100%sequence homology.This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City,and supports the notion that swine are a source of human HEV infection.
