Singlet oxygen(^(1)O_(2))is a highly reactive oxygen species involved in numerous chemical and photochemical reactions in diferent biological systems and in particular,in photodynamic therapy(PDT).However,the quantifc...Singlet oxygen(^(1)O_(2))is a highly reactive oxygen species involved in numerous chemical and photochemical reactions in diferent biological systems and in particular,in photodynamic therapy(PDT).However,the quantifcation of^(1)O_(2) generation during in vitro and in vivo pho-tosensitization is still technically challenging.To address this problem,indirect and direct methods for^(1)O_(2)detection have been intensively studied.This review presents the available methods currently in use or under development for detecting and quantifying^(1)O_(2) generation during photosensitization.The advantages and limitations of each method will be presented.Moreover,the future trends in developing PDT-^(1)O_(2) dosimetry will be briefly discussed.展开更多
Excited-state singlet axygen(^(1)O_(2)),generated during photodynamic therapy(PDT),is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers.Its relative concentration in cell...Excited-state singlet axygen(^(1)O_(2)),generated during photodynamic therapy(PDT),is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers.Its relative concentration in cells or tissues can be measured directly through its near-infrared(NIR)luminescence emission,which has correlated well with in vitro cell and in triro normal skin treatment responses.Here,its correlation with the response of tumor tiss1e in vito is examined,using the photosensitizer meso-tetralydroxyphenylchlorin(mTHPC)in an animal model comprising luciferase-and green fluorescent protein(GFP)-transduced gliosarcoma grown in a dorsal window chamber.The change in the bioluminescence signal,imaged pre-treatment and at 2,5 and 9 d post treatment,was used as a quantitative measure of the tumor response,which was classified in individual tumors as"non","moderate"and"strong"in order to reduce the variance in the data.Plotting the bioluminescence-based response vs the^(1)O_(2)counts demonstrated clear correlation,indicating tha^(1)O_(2)luminescence provides a valid do-simetric technique for PDT in tumor tissue.展开更多
Simultaneous metabolic and oxygen imaging is promising to follow up therapy response,dis-ease development and to determine prognostic factors.FLIM of metabolic coenzymes is now widely accepted to be the most reliable ...Simultaneous metabolic and oxygen imaging is promising to follow up therapy response,dis-ease development and to determine prognostic factors.FLIM of metabolic coenzymes is now widely accepted to be the most reliable method to determine cellular bioenergetics.Also,oxygen consumption has to be taken into account to understand treatment responses.The phosphorescence lifetimne of oxygen sensors is able to indicate local oxygen changes.For phosphorescence lifetime imaging(PLIM)dyes based on ruthenium(I)coordination com-plexes are useful,in detaill TLD1433 which possesses a variety of different triplet states,enables complex photochemistry and redox reactions.PLIM is usally reached by two photon exci-tation of the drug with a femtosecond(fs)pulsed Ti:Sapphire laser working at 80 MHz repe-tition rate and(time-correlated single photon counting)(TCSPC)detection electronics.The interesting question was whether it is possible to follow up PLIM 1using faster repetition rates.Faster repetition rates could be advantageous for the induction of specific photochemical reactions because of similar light doses used normally in standard CW light treatments.For this,a default 2p-FLIM-PLIM system was expanded by adding a second fs pulsed laser("helixx")which provides 50 fs pulses at a repetition rate of 250 MHz,more than 2.3 w average power and tunable from 720 nm to 920 nm.The laser beam was coupled into the AOM instead of the default 80 MHz laser.We demonstrated siuccessful applications of the 250 MHz laser for PLIM which correlates well with measurements done by excitation with the conventional 80MHx laser source.展开更多
基金supported by the National Natural Science Foundation of China(60978070,61036014,61175216)the Natural Science Foundation for Dis-tinguished Young Scholars of Fujian Province(2011J06022)+1 种基金the program for New Century Excel-lent Talents in University of China(NCET-10-0012)the Program for Changjiang Scholars and Inno-vative Research Team in University(IRT1115).
文摘Singlet oxygen(^(1)O_(2))is a highly reactive oxygen species involved in numerous chemical and photochemical reactions in diferent biological systems and in particular,in photodynamic therapy(PDT).However,the quantifcation of^(1)O_(2) generation during in vitro and in vivo pho-tosensitization is still technically challenging.To address this problem,indirect and direct methods for^(1)O_(2)detection have been intensively studied.This review presents the available methods currently in use or under development for detecting and quantifying^(1)O_(2) generation during photosensitization.The advantages and limitations of each method will be presented.Moreover,the future trends in developing PDT-^(1)O_(2) dosimetry will be briefly discussed.
基金supported by the Canadian Cancer Society Research Institute (014245)the Fujian Provincial Natural Science Foundation (2011J06022).
文摘Excited-state singlet axygen(^(1)O_(2)),generated during photodynamic therapy(PDT),is believed to be the primary cytotoxic agent with a number of clinically approved photosensitizers.Its relative concentration in cells or tissues can be measured directly through its near-infrared(NIR)luminescence emission,which has correlated well with in vitro cell and in triro normal skin treatment responses.Here,its correlation with the response of tumor tiss1e in vito is examined,using the photosensitizer meso-tetralydroxyphenylchlorin(mTHPC)in an animal model comprising luciferase-and green fluorescent protein(GFP)-transduced gliosarcoma grown in a dorsal window chamber.The change in the bioluminescence signal,imaged pre-treatment and at 2,5 and 9 d post treatment,was used as a quantitative measure of the tumor response,which was classified in individual tumors as"non","moderate"and"strong"in order to reduce the variance in the data.Plotting the bioluminescence-based response vs the^(1)O_(2)counts demonstrated clear correlation,indicating tha^(1)O_(2)luminescence provides a valid do-simetric technique for PDT in tumor tissue.
基金supported by the Ministry of Research and Development,FKZ order:13N14508("OMOXI")by the Ministry of Economics,ZIM-Project,FKZ:ZF4322901RE6("UFEMPU").
文摘Simultaneous metabolic and oxygen imaging is promising to follow up therapy response,dis-ease development and to determine prognostic factors.FLIM of metabolic coenzymes is now widely accepted to be the most reliable method to determine cellular bioenergetics.Also,oxygen consumption has to be taken into account to understand treatment responses.The phosphorescence lifetimne of oxygen sensors is able to indicate local oxygen changes.For phosphorescence lifetime imaging(PLIM)dyes based on ruthenium(I)coordination com-plexes are useful,in detaill TLD1433 which possesses a variety of different triplet states,enables complex photochemistry and redox reactions.PLIM is usally reached by two photon exci-tation of the drug with a femtosecond(fs)pulsed Ti:Sapphire laser working at 80 MHz repe-tition rate and(time-correlated single photon counting)(TCSPC)detection electronics.The interesting question was whether it is possible to follow up PLIM 1using faster repetition rates.Faster repetition rates could be advantageous for the induction of specific photochemical reactions because of similar light doses used normally in standard CW light treatments.For this,a default 2p-FLIM-PLIM system was expanded by adding a second fs pulsed laser("helixx")which provides 50 fs pulses at a repetition rate of 250 MHz,more than 2.3 w average power and tunable from 720 nm to 920 nm.The laser beam was coupled into the AOM instead of the default 80 MHz laser.We demonstrated siuccessful applications of the 250 MHz laser for PLIM which correlates well with measurements done by excitation with the conventional 80MHx laser source.
