In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verifi...In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verified by colony PCR of the （AG）n microsatellite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsatellites were obtained, accounting for 86.7%. Discarding sequences with miscella- neous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 im- perfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp. , which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp.展开更多
Thirty bloomed hybrids of C. azalea were selected to have been studied. Of them, 10 cross-combinations were included. The results showed that the hybrids were consistently tended to C. azalea on flower bud formation a...Thirty bloomed hybrids of C. azalea were selected to have been studied. Of them, 10 cross-combinations were included. The results showed that the hybrids were consistently tended to C. azalea on flower bud formation and development, blooming period, leaf trait and plant growth, but flower color, flower form and flower size of the hybrids, in a great degree, were dependent on the traits of another non-C, azalea＇s cross-parent. The hybrids all can bloom from summer to December and even to next spring. The hybrids＇ photographs illustrated in the paper show that their flowers are beautiful with different color, form and size. Also some of genetic laws of C. azalea＇s hybrids were discussed. These fire-new hybrids firstly fill the blank that no camellia flowers can be enjoyed in summer in the world.展开更多
Two unknown toxic diterpenoids,rhodomollein-I and rhodompllein-Ⅱ,and the known rhodojaponin-Ⅲ,have been isolated from Chinese azalea (Ericaceae,Rhododendron molle G.Don). rhodomollein-I,C_(20)H_(32)O_6,colorless thi...Two unknown toxic diterpenoids,rhodomollein-I and rhodompllein-Ⅱ,and the known rhodojaponin-Ⅲ,have been isolated from Chinese azalea (Ericaceae,Rhododendron molle G.Don). rhodomollein-I,C_(20)H_(32)O_6,colorless thin needles,[a]D^(20)-6.65 (c 3.76×10^(-2),EtOH),m.p.241— 242.5℃.Its structure has been established as 1 through spectral analysis of ^(13)C NMR,~1H NMR, MS,IR and UV.Moreover,its stereostructure was unambiguously identified from the different threshold level line of ~1H—~1H NOESY.展开更多
基金Supported by Doctoral Starting up Foundation of Huanggang Normal University(2013030903)Opening Fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization(2013000403)
文摘In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verified by colony PCR of the （AG）n microsatellite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsatellites were obtained, accounting for 86.7%. Discarding sequences with miscella- neous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 im- perfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp. , which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp.
文摘Thirty bloomed hybrids of C. azalea were selected to have been studied. Of them, 10 cross-combinations were included. The results showed that the hybrids were consistently tended to C. azalea on flower bud formation and development, blooming period, leaf trait and plant growth, but flower color, flower form and flower size of the hybrids, in a great degree, were dependent on the traits of another non-C, azalea＇s cross-parent. The hybrids all can bloom from summer to December and even to next spring. The hybrids＇ photographs illustrated in the paper show that their flowers are beautiful with different color, form and size. Also some of genetic laws of C. azalea＇s hybrids were discussed. These fire-new hybrids firstly fill the blank that no camellia flowers can be enjoyed in summer in the world.
文摘Two unknown toxic diterpenoids,rhodomollein-I and rhodompllein-Ⅱ,and the known rhodojaponin-Ⅲ,have been isolated from Chinese azalea (Ericaceae,Rhododendron molle G.Don). rhodomollein-I,C_(20)H_(32)O_6,colorless thin needles,[a]D^(20)-6.65 (c 3.76×10^(-2),EtOH),m.p.241— 242.5℃.Its structure has been established as 1 through spectral analysis of ^(13)C NMR,~1H NMR, MS,IR and UV.Moreover,its stereostructure was unambiguously identified from the different threshold level line of ~1H—~1H NOESY.
