期刊文献+
共找到19篇文章
< 1 >
每页显示 20 50 100
核转录因子κB p65和骨桥蛋白在子宫内膜异位症中的表达及相关性研究 被引量:9
1
作者 白治苗 李望舒 +3 位作者 姚卫卫 卢玉凤 杨眉 哈春芳 《中国全科医学》 CAS CSCD 北大核心 2016年第24期2915-2919,共5页
背景目前子宫内膜异位症(EMs)的病因和发病机制尚不清楚,治疗措施有限,严重影响女性的身心健康。目的探讨核转录因子κB(NF-κB)p65和骨桥蛋白(OPN)在EMs组织中的表达及二者的相关性。方法收集2013年1—12月于宁夏医科大学总医院妇产科... 背景目前子宫内膜异位症(EMs)的病因和发病机制尚不清楚,治疗措施有限,严重影响女性的身心健康。目的探讨核转录因子κB(NF-κB)p65和骨桥蛋白(OPN)在EMs组织中的表达及二者的相关性。方法收集2013年1—12月于宁夏医科大学总医院妇产科因EMs行手术的患者35例(病例组),于术中或术后确诊,其中增生期17例,分泌期18例;收集同期行双侧输卵管结扎手术并经病理科诊断排除EMs的正常子宫内膜者30例作为对照(正常子宫内膜组),其中增生期15例,分泌期15例。分别采用免疫组化SP法、RT-PCR法检测正常子宫内膜组、EMs在位内膜组和EMs异位内膜组NF-κB p65、OPN及其mRNA的表达。结果 NF-κB p65、OPN主要表达于子宫内膜腺上皮细胞的胞质中,极少数表达于间质细胞中。EMs在位、异位内膜组NF-κB p65和OPN表达高于正常子宫内膜组,EMs异位内膜组高于EMs在位内膜组(P<0.05)。3组增生期及分泌期NF-κB p65的表达比较,差异无统计学意义(P>0.05),EMs在位、异位内膜组分泌期OPN的表达均高于增生期(P<0.05);EMs在位内膜组和EMs异位内膜组中NF-κB与OPN的表达均呈正相关(r在位=0.88,r异位=0.78,P<0.05)。NF-κB p65、OPN mRNA在EMs在位、异位内膜组中的表达高于正常子宫内膜组,EMs异位内膜组高于EMs在位内膜组(P<0.05)。结论 NF-κB p65、OPN及其mRNA在EMs患者在位、异位内膜组织的表达明显增强,并呈正相关,提示二者极有可能通过相互激活进而诱导EMs的发生、发展。 展开更多
关键词 子宫内膜异位症 因子Κb 骨桥蛋白质
下载PDF
血管内皮生长因子-B与眼部新生血管性疾病
2
作者 马慧蕾(综述) 孙大卫(审校) 《中华实验眼科杂志》 CAS CSCD 北大核心 2014年第5期476-480,共5页
异常的新生血管会导致多种眼部疾病,而血管内皮生长因子(VEGF)在新生血管的发生及发展中起重要作用.VEGF-B作为VEGF家族的一员,对血管生成和血管通透性无显著影响,但可通过血管生存作用和细胞凋亡作用对新生血管的生长进行调控,从而... 异常的新生血管会导致多种眼部疾病,而血管内皮生长因子(VEGF)在新生血管的发生及发展中起重要作用.VEGF-B作为VEGF家族的一员,对血管生成和血管通透性无显著影响,但可通过血管生存作用和细胞凋亡作用对新生血管的生长进行调控,从而抑制新生血管的发生和发展.同时,VEGF-B对心脏和神经元等具有保护作用.抗VEGF治疗作为目前明确有效的治疗新生血管的方法,一直受到广泛关注,而因VEGF-B的两面性,对于抗VEGF-B的药物在治疗眼科新生血管性疾病方面的作用尚需进一步研究.就VEGF-B对机体血管、细胞、神经元及心脏所起的作用及其在眼部新生血管性疾病治疗中的应用进行综述. 展开更多
关键词 血管内皮生长因子-b 新生血管性疾病 治疗
下载PDF
最小度和[a,b」——覆盖图
3
作者 何乐亮 《泰山学院学报》 1997年第6期16-18,共3页
设 a≤ b是整数,G=(V(G),E(G))是一个图G的一个支撑子图F称为G的一个[a,b]—因子,若对任意的v∈V(G),有a≤d_F,(v)≤b.图G称为是[a,b]—覆盖图,若对G的每一条边,存在G的一个[a,b])—因子包含它,本文给出了一个图是[a,b]—覆盖图的关于... 设 a≤ b是整数,G=(V(G),E(G))是一个图G的一个支撑子图F称为G的一个[a,b]—因子,若对任意的v∈V(G),有a≤d_F,(v)≤b.图G称为是[a,b]—覆盖图,若对G的每一条边,存在G的一个[a,b])—因子包含它,本文给出了一个图是[a,b]—覆盖图的关于最小度的充分条件,证明了下列结果;设1≤a<b是整数,G是一个阶为n图.如果图G的最小度(?)(G)>n+(a+b)-2(bn-1)^(1/2)则G是一个[a,b]—覆盖图. 展开更多
关键词 图论 [a b]—因子 [a b]—覆盖图 最小度
下载PDF
在体大鼠心肌缺血再灌注损伤中NF-κBp65与Ang Ⅱ表达的变化 被引量:5
4
作者 杨锐 王芬珍 《心电与循环》 2014年第2期123-126,142,共5页
目的观察在体大鼠心肌缺血的不同再灌注时程NF-κBp65与AngⅡ表达的变化。方法 40只大鼠采用随机数字表法分为R_(15min)组、R_(30min)组、R_(60min)组、R_(120min)组及对照组。经缺血30min及不同时间的再灌注(15,30,60,120 min)而建立... 目的观察在体大鼠心肌缺血的不同再灌注时程NF-κBp65与AngⅡ表达的变化。方法 40只大鼠采用随机数字表法分为R_(15min)组、R_(30min)组、R_(60min)组、R_(120min)组及对照组。经缺血30min及不同时间的再灌注(15,30,60,120 min)而建立在体心肌缺血再灌注损伤模型。以ELISA法定量检测NF-κBp65蛋白的DNA结合活性;采用放射免疫分析法检测血浆及心肌组织AngⅡ含量。通过Pearson相关性检验对NF-κBp65与循环及心肌局部的AngⅡ含量做相关性分析。结果 NF-κBp65蛋白表达量随再灌注时间的延长呈升高趋势,于R_(60min)时达高峰,R_(120min)组与R_(60min)组比有所下降,差异均有统计学意义(均P<0.05),但仍高于R_(30min)组;各实验组血浆及心肌局部AngⅡ含量与对照组比明显升高,随再灌注时间的延长呈升高趋势,差异有统计学意义(P<0.05),两者呈直线正相关(r=0.859、0.809,均P<0.05)。结论心肌缺血再灌注损伤时NF-κBp65及血液与心肌AngⅡ均增高。 展开更多
关键词 缺血再灌注损伤 血管紧张素Ⅱ
下载PDF
Lipopolysaccharide-induced cerebral inflammatory damage and the therapeutic effect of platelet activating factor receptor antoganist
5
作者 刘文超 丁文龙 +2 位作者 顾红玉 陈明峰 胡金家 《Neuroscience Bulletin》 SCIE CAS CSCD 2007年第5期271-276,共6页
Objective To investigate lipopolysaccharide (LPS) induced acute cerebral inflammatory damage and the therapeutic effect of ginkgolide B (BN52021). Methods Thirty Sprague-Dawley rats were randomly divided into 3 gr... Objective To investigate lipopolysaccharide (LPS) induced acute cerebral inflammatory damage and the therapeutic effect of ginkgolide B (BN52021). Methods Thirty Sprague-Dawley rats were randomly divided into 3 groups (n = 10 for each group): Control group, Model group and Treatment group (treated with BN52021). LPS were injected into the fourth ventricle of rat to make a neuroinflammatory murine model. Morris water maze was used to detect the learning and memory ability of rats; changes of synapse number and subcellular ultrastructures were observed under a transmission electron microscope; OX-42 positive microglia in the brain was detected by immunohistochemical method. Results The average escape latency in the Treatment group were significantly shortened than that in the Model group; and the percentage of swimming distance traveled in platform quadrant accounting for total distance increased markedly. The rough endoplasmic reticulum and polyribosomes in the Treatment group were more than that in the Model group, but the number of synapses seemed to have no obvious change. The number of OX-42 positive microglia in the Treatment group decreased markedly than that in the Model group, and the grey density of OX-42-positive cells increased significantly. Conclusion LPS can induce inflammatory damages to the brain, but the damage could be antagonized by BN52021. Platelet activating factor receptor antagonist may offer an effective therapy for neurodegeneration diseases. 展开更多
关键词 brain inflammation platelet activating factor ginkgolide b ULTRASTRUCTURE MICROGLIA
下载PDF
Effect of resveratrol on activation of nuclear factor kappa-B and inflammatory factors in rat model of acute pancreatitis 被引量:44
6
作者 YongMeng Qing-YongMa +1 位作者 Xiao-PingKou JunXu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第4期525-528,共4页
AIM: To observe the effect of resveratrol on nuclear factor Kappa-B (NF-κB) activation and the inflammatory response in sodium taurocholate-induced pancreatitis in rats. METHODS: Seventy-two male SD rats were randoml... AIM: To observe the effect of resveratrol on nuclear factor Kappa-B (NF-κB) activation and the inflammatory response in sodium taurocholate-induced pancreatitis in rats. METHODS: Seventy-two male SD rats were randomly divided into three groups: sham operation group (control), severe acute pancreatitis (SAP) group, and severe acute pancreatitis group treated with resveratrol (RES). A SAP model was established by injecting 4% sodium taurocholate 1 mL/kg through puncturing the pancreatic duct. In Res group, Res was given at 30 mg/kg b.m. intraperitoneally after the SAP model was successfully established. Eight animals from each group were sacrificed at 3, 6 and 12 h after modeling. The expression of NF-κB activation of pancreas was detected by irnmunohistochemical staining, whereas the levels of TNF-α and IL-8 in pancreatic tissues were estimated by radioimrnunoassay. The pathological changes of pancreas and lungs were examined microscopically. RESULTS: Much less hyperemia, edema, dust-colored necrotic focus and soaps were noticed in pancreas in RES group than in SAP group. In RES group, hemorrhage, exudates and infiltration of inflammatory cells in pancreas and interstitial edema, destruction of alveolar wall in lung were significantly less than in SAP group. In the SAP group, the activation of NF-κB in pancreatic tissues was enhanced significantly at any measure point compared with control group (64.23±10.72% vs 2.56±0.65%, 55.86±11.34% vs 2.32±0.42%, 36.23±2.30% vs 2.40±0.36%,P<0.01), TNF-α, IL-8 were also increased and reached their peak at 6 h and then declined. The activation of NF-κB and the levels of TNF-α and IL-8 in RES group were significantly lower than those in SAP group (P<0.01): activation (52.63±9.45% vs 64.23±10.72%, 40.52±8.40% vs 55.86±11.34%, 29.83±5.37% vs 36.23±2.30%), TNF-α (132.76±15.68 pg/mL vs 158.36±12.58 pg/mL, 220.32±23.57 pg/mL vs 247.67± 11.62 pg/mL, 175.68±18.43 pg/mL vs 197.35±12.57 pg/mL) and IL-8 (0.62±0.21 μg/L vs 0.83±0.10 μg/L, 1.10±0.124 μg/L vs1.32±0.18 μg/L, 0.98±0.16 μg/L vs 1.27±0.23μg/L). CONCLUSION: The activation of NF-KB is involved in the inflammatory response of rats with SAP. Resveratrol could effectively inhibit the expression of NF-κB activation, alleviate the severity of SAP through its anti-inflammatory effects and regulate the inflammatory mediators. 展开更多
关键词 Acute pancreatitis RESVERATROL NF-Κb Inflammatory factors
下载PDF
Molecular Phylogeny of Slow Lorises (Nycticebus) Revealed by D-loop Sequences and Complete Cytochrome b Gene Sequences of Mitochondrial DNA
7
作者 陈静华 Paul CRO W +2 位作者 成岛悦雄 张红卫 张亚平 《Zoological Research》 CAS CSCD 北大核心 2004年第4期292-297,共6页
Partial sequences of the D-loop and the complete sequences of cytochrome b gene (1 140 bp) of the slow lorises (genus Nycticebus) were undertaken to investigate evolutionary relationships among species of Nycticebus.S... Partial sequences of the D-loop and the complete sequences of cytochrome b gene (1 140 bp) of the slow lorises (genus Nycticebus) were undertaken to investigate evolutionary relationships among species of Nycticebus.Sequence analysis results consistently provide new taxonomy evidence at the DNA level for supporting Ratajszczak and Groves’ viewpoint that N.intermedus is merely the adult of N.pygmaeus (Ratajszczak,1998;Groves,1971).Phylogenetic analysis was performed by means of the combined data and these two separate sequences data,respectively,by using various methods,supporting the same topology,in which genus Nycticebus was formed of two clusters.The first cluster was composed of N.pygmaeus,and the second cluster of N.coucang.It also could provide a new molecular genetic evidence to support the view that the genus comprises two species:N.coucang and N.pygmaeus. 展开更多
关键词 Nycticebus Mitochondiral DNA Cytochrome b gene D-LOOP Molecular phylogeny
下载PDF
Hydrogen sulfide attenuates gastric mucosal injury induced by restraint water-immersion stress via activation of KATPchannel and NF-κB dependent pathway 被引量:7
8
作者 Hong-Zhao Sun Shan Zheng +4 位作者 Kai Lu Feng-Tian Hou Jie-Xue Bi Xue-Lian Liu Shan-Shan Wang 《World Journal of Gastroenterology》 SCIE CAS 2017年第1期87-92,共6页
AIMTo explore the effect of hydrogen sulfide (H<sub>2</sub>S) on restraint water-immersion stress (RWIS)-induced gastric lesions in rats and the influence of adenosine triphosphate (ATP)-sensitive potassiu... AIMTo explore the effect of hydrogen sulfide (H<sub>2</sub>S) on restraint water-immersion stress (RWIS)-induced gastric lesions in rats and the influence of adenosine triphosphate (ATP)-sensitive potassium (K<sub>ATP</sub>) channels and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-&#x003ba;B) pathway on such an effect.METHODSMale Wistar rats were randomly divided into a control group, a physiological saline (PS) group, a sodium hydrosulfide (NaHS) group, a glibenclamide (Gl) group, Gl plus NaHS group, a pyrrolidine dithiocarbamate (PDTC) group, and a PDTC plus NaHS group. Gastric mucosal injury was induced by RWIS for 3 h in rats, and gastric mucosal damage was analyzed after that. The PS, NaHS (100 &#x003bc;mol/kg body weight), Gl (100 &#x003bc;mol/kg body weight), Gl (100 &#x003bc;mol/kg or 150 &#x003bc;mol/kg body weight) plus NaHS (100 &#x003bc;mol/kg body weight), PDTC (100 &#x003bc;mol/kg body weight), and PDTC (100 &#x003bc;mol/kg body weight) plus NaHS (100 &#x003bc;mol/kg body weight) were respectively injected intravenously before RWIS.RESULTSRWIS induced serious gastric lesions in the rats in the PS pretreatment group. The pretreatment of NaHS (a H<sub>2</sub>S donor) significantly reduced the damage induced by RWIS. The gastric protective effect of the NaHS during RWIS was attenuated by PDTC, an NF-&#x003ba;B inhibitor, and also by glibenclamide, an ATP-sensitive potassium channel blocker, in a dose-dependent manner.CONCLUSIONThese results suggest that exogenous H<sub>2</sub>S plays a protective role against RWIS injury in rats, possibly through modulation of K<sub>ATP</sub> channel opening and the NF-&#x003ba;B dependent pathway. 展开更多
关键词 Hydrogen sulfide Nuclear factor kappa b Gastric mucosal injury Restraint water-immersion stress Adenosine triphosphate-sensitive potassium
下载PDF
Molecular Evolution of the Cytochrome b Gene in Geographically Isolated Populations of Oryzias Latipes
9
作者 Nobuhiko Asada Akira Hirai +3 位作者 Taiki Hamada Eri Sato Akio Moriyama Tetsuro Takeuchi 《Journal of Life Sciences》 2011年第4期282-286,共5页
The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3... The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3' ends was 1,143 base pairs (bp) and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control (Hd-rR strain) and HS strains than the K-I 1 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 (K-I I strain) and AB480879 (HS strain). 展开更多
关键词 Cytochrome b RFLP DIVERSITY EVOLUTION Oryzias latipes.
下载PDF
Admission white blood cell count predicts short-term clinical outcomes in patients with uncomplicated Stanford type B acute aortic dissection 被引量:4
10
作者 Zhao-Ran CHEN Bi HUANG +4 位作者 Hai-Song LU Zhen-Hua ZHAO Ru-Tai HUI Yan-Min YANG Xiao-Han FAN 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2017年第1期49-56,共8页
Objectives Inflammation has been shown to be related with acute aortic dissection (AAD). The present study aimed to evaluate the association of white blood cell counts (WI3Cc) on admission with both in-hospital an... Objectives Inflammation has been shown to be related with acute aortic dissection (AAD). The present study aimed to evaluate the association of white blood cell counts (WI3Cc) on admission with both in-hospital and long-term all-cause mortality in patients with uncom- plicated Stanford type B AAD. Methods From 2008 to 2010, a total of 377 consecutive patients with uncomplicated type B AAD were enrolled and then followed up. Clinical data and WBCc on admission were collected. The primary end points were in-hospital death and long-term all-cause death. Results The in-hospital death rate was 4.2%, and the long-term all-cause mortality rate was 6.9% during a median follow-up of 18.9 months. WBCc on admission was identified as a risk factor for in-hospital death by univariate Cox regression analysis as both a continuous variable and a categorical variable using a cut off of 11.0 × 109 cell/L (all P 〈 0.05). After adjusting for age, sex and other risk factors, elevated admission WBCc was still a significant predictor for in-hospital death as both a continuous variable [hazard ratio (HR): 1.052, 95% CI: 1.024-1.336, P = 0.002] and a categorical variable using a cut off of 11.0 × 109 cell/L (HR: 2.056, 95% CI: 1.673-5.253, P = 0.034). No relationship was observed between WBCc on admission and long-term all-cause death. Conclusions Our results indicate that elevated WBCc upon admission might be used as a predictor for increased risk of in-hospital death in uncomplicated type B AAD. There might be no predictive value of WBCc for the long-term survival of type B AAD. 展开更多
关键词 Acute aortic dissection In-hospital mortality Stanford type b SURVIVAL White blood cell
下载PDF
Overexpression of metastasis-associated in colon cancer 1 predicts a poor outcome of hepatitis B virus-related hepatocellular carcinoma 被引量:6
11
作者 Jian-Hui Qu Xiu-Juan Chang +12 位作者 Yin-Ying Lu Wen-Lin Bai Yan Chen Lin Zhou Zhen Zeng Chun-Ping Wang Lin-Jing An Li-Yan Hao Gui-Lin Xu Xu-Dong Gao Min Lou Ji-Yun Lv Yong-Ping Yang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第23期2995-3003,共9页
AIM: To investigate the intratumoral expression of metastasis-associated in colon cancer 1 (MACC1) and c-Met and determine their clinical values associated with hepatitis B virus (HBV)-related hepatocellular carcinoma... AIM: To investigate the intratumoral expression of metastasis-associated in colon cancer 1 (MACC1) and c-Met and determine their clinical values associated with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). METHODS: A retrospective study admitted three hundred fifty-four patients with HBV-related HCC. The expression and distribution of MACC1 and c-Met were assessed by quantitative real-time polymerase chain reaction and immunohistochemistry staining. Prognostic factors influencing survival, metastasis and recurrence were assessed. RESULTS: Intratumoral MACC1 level was found to be associated with HCC disease progression. Both median tumor-free survival (TFS) and overall survival (OS) were significantly shorter in the postoperative HCC patients with high intratumoral MACC1 expression, as compared to those with low intratumoral MACC1 levels (TFS: 34 mo vs 48.0 mo, P < 0.001; OS: 40 mo vs 48 mo, P < 0.01). Multivariable analysis indicated that high MACC1 expression or co-expression with c-Met were independent predictors for HCC clinic outcome (P < 0.001). CONCLUSION: High intratumoral MACC1 expression can be associated with enhanced tumor progression and poor outcome of HBV-related HCC. MACC1 may serve as a prognostic biomarker for postoperative HCC. 展开更多
关键词 Hepatocellular carcinoma Metastasis-as-sociated in colon cancer 1 c-Met Prognostic factor Recurrence
下载PDF
Modulation of B-cell receptor and microenvironment signaling by a guanine exchange factor in B-cell malignancies
12
作者 Wei Liao Sanjai Sharma 《Cancer Biology & Medicine》 SCIE CAS CSCD 2016年第2期277-285,共9页
Objective: Chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) cells over-express a guanine exchange factor (GEF), Rasgrf-1. This GEF increases active Ras as it catalyzes the removal of GDP from R... Objective: Chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) cells over-express a guanine exchange factor (GEF), Rasgrf-1. This GEF increases active Ras as it catalyzes the removal of GDP from Ras so that GTP can bind and activate Ras. This study aims to study the mechanism of action of Rasgrf-1 in B-cell malignancies. Methods: N-terminus truncated Rasgrf-1 variants have a higher GEF activity as compared to the full-length transcript therefore a MCL cell line with stable over-expression of truncated Rasgrf-1 was established. The B-cell receptor (BCR) and chemokine signaling pathways were compared in the Rasgrf-I over-expressing and a control transfected cell line. Results: Cells over-expressing truncated form of Rasgrf-1 have a higher proliferative rate as compared to control transfected cells. BCR was activated by lower concentrations of anti-IgM antibody in Rasgrf-1 over-expressing cells as compared to control cells indicating that these cells are more sensitive to BCR signaling. BCR signaling also phosphorylates Rasgrf-1 that further increases its GEF function and amplifies BCR signaling. This activation of Rasgrf-1 in over-expressing cells resulted in a higher expression of phospho-ERK, AKT, BTK and PKC-alpha as compared to control cells. Besides BCR, Rasgrf-1 over-expressing cells were also more sensitive to microenvironment stimuli as determined by resistance to apoptosis, chemotaxis and ERK pathway activation. Conclusions: This GEF protein sensitizes B-cells to BCR and chemokine mediated signaling and also upregulates a number of other signaling pathways which promotes growth and survival of these cells. 展开更多
关键词 b-ceU malignancies mantle cell lymphoma chronic lymphocytic leukemia (CLL) b-cell receptor guanine exchange factorRasgrf- 1 ERK pathway
下载PDF
Mitochondrial DNA Sequences Imply Anhui Musk Deer a Valid Species in Genus Moschus 被引量:13
13
作者 宿兵 王应祥 王岐山 《Zoological Research》 CAS CSCD 北大核心 2001年第3期169-173,共5页
Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b ge... Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies. 展开更多
关键词 Musk deer Genus Moschus Anhui musk deer Cytochrome b gene Molecular phylogeny
下载PDF
利拉鲁肽对2型糖尿病大鼠NF-κB及VCAM-1表达的影响 被引量:7
14
作者 綦才辉 金勇君 +3 位作者 杨美子 徐鑫淼 张凌云 曹鹏娟 《山东大学学报(医学版)》 CAS 北大核心 2014年第4期39-42,48,共5页
目的观察利拉鲁肽对2型糖尿病大鼠主动脉核因子-κB(NF-κB)及血管细胞间黏附因子(VCAM-1)表达的影响及可能机制。方法高脂高糖饲料喂养联合小剂量链脲佐菌素腹腔注射,建立2型糖尿病大鼠模型。40只8周龄雄性SD大鼠分为4组:正常对照组(NC... 目的观察利拉鲁肽对2型糖尿病大鼠主动脉核因子-κB(NF-κB)及血管细胞间黏附因子(VCAM-1)表达的影响及可能机制。方法高脂高糖饲料喂养联合小剂量链脲佐菌素腹腔注射,建立2型糖尿病大鼠模型。40只8周龄雄性SD大鼠分为4组:正常对照组(NC组)、糖尿病对照组(DM组)、格列本脲对照组(DMG组)及利拉鲁肽干预治疗组(DML组)。DMG组及DML组分别给予格列本脲及利拉鲁肽干预治疗8周。12周末腹主动脉取血分离血清,测空腹血糖(FBG)、空腹胰岛素(FIns)、甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)及高密度脂蛋白(HDL-C);分离大鼠主动脉,石蜡切片HE染色观察大鼠主动脉形态学变化;免疫组化方法(SP法)测大鼠主动脉NF-κB及VCAM-1的表达。结果与DM组相比,DMG组及DML组大鼠FIns及胰岛素抵抗指数(HOMA-IR)明显降低(P均<0.01);与DMG组相比,DML组大鼠FIns、HOMA-IR、TG及LDL-C明显降低(P均<0.05),主动脉NF-κB和VCAM-1的表达降低(P<0.05)。结论利拉鲁肽可下调糖尿病大鼠主动脉NF-κB和VCAM-1的表达,调节糖脂代谢。此过程可改善大血管并发症。 展开更多
关键词 利拉鲁肽 糖尿病大鼠 血管细胞间黏附因子1 VASCULAR cell adhesion MOLECULE 1
原文传递
Effect of moxibustion on tumor necrosis factor-α and nuclear transcription factor kappa B in ankle joints of rats with rheumatoid arthritis 被引量:14
15
作者 张慧 马晓芃 +7 位作者 吴焕淦 吴生兵 苏三山 胡阳春 李振峰 张丽 解明月 杨燕萍 《Journal of Acupuncture and Tuina Science》 CSCD 2017年第3期171-176,共6页
Objective: To observe the effect of moxibustion on the expressions of tumor necrosis factor-α(TNF-α) and nuclear transcription factor kappa B(NF-κB) proteins in ankle joints of rats with rheumatoid arthritis... Objective: To observe the effect of moxibustion on the expressions of tumor necrosis factor-α(TNF-α) and nuclear transcription factor kappa B(NF-κB) proteins in ankle joints of rats with rheumatoid arthritis(RA), and to explore the anti-inflammatory mechanism of moxibustion in the treatment of RA. Methods: Adjuvant arthritis(AA) rat models were induced and used as rat models of RA. Rats were randomly divided into a normal group, a model group, a moxibustion group and a saline group. Rats in the normal and model groups were not treated; rats in the moxibustion group accepted treatment by moxibustion at Zusanli(ST 36) and Shenshu(BL 23) after modeling; rats in the saline group were injected with 0.15 mL saline to the bottom of the left hind paw and had no other treatments. Hematoxylin-eosin(HE) staining was used to observe the histopathological changes of rats' ankle joints under light microscope. Immunohistochemistry was used to observe the expressions of TNF-α and NF-κB in ankle joints of rats. Results: Compared with the normal group, the rats' ankle joints in the model group showed disorganization, joint surface defect, and significantly increased mean optical density(MOD) of TNF-α and NF-κB(all P〈0.05). After moxibustion treatment, rats in the moxibustion group showed repaired ankle tissues, smooth joint surface without defects, decreased MOD of TNF-α and NF-κB than those in the model group(all P〈0.05). Rats in the saline group had no damage to ankle joints, while TNF-α and NF-κB were significantly different from those in the model group(P〈0.05). Conclusion: Moxibustion could down-regulate the expressions of NF-κB and TNF-α protein in ankle joints of RA rats, and produce an anti-inflammatory effect to promote tissue repair. 展开更多
关键词 Moxibustion Therapy Moxa Stick Moxibustion ARTHRITIS RHEUMATOID Tumor Necrosis Factor-alpha NF-kappa b RATS
原文传递
Functional characterization of the promoter of carbonyl reductase 1 gene in porcine endometrial cells 被引量:1
16
作者 Ai-ling ZHANG Xian-yue SUN +4 位作者 Qi YIN Jian-hua ZENG Zhe ZHANG Jia-qi LI Hao ZHANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2017年第7期626-634,共9页
Prostaglandins (PGs) play a critical role in porcine reproduction, of which prostaglandin E2 (PGE2) and prostaglandin F2a (PGF2a) exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enz... Prostaglandins (PGs) play a critical role in porcine reproduction, of which prostaglandin E2 (PGE2) and prostaglandin F2a (PGF2a) exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enzyme, carbonyl reductase 1 (CBR1) catalyzes the conversion of PGE2 to PGF2a. A high ratio of PGE2:PGF2a is beneficial to the establishment and maintenance of porcine pregnancy. PG is essential for the establishment of pregnancy which resembles the proinflammatory response and nuclear factor KB (NF-KB) is involved in the process. Bioinformatic analysis has shown that NF-KB is a possible factor bound to two cis-regulatory elements in CBRI promoter. In this study, we cloned the 2997 bp (-2875/+122) of the promoter, and constructed six 5'-deleted dual-luciferase reporter recombinant vectors. In endometrial cells, the region of P2 (-16401+7) exhibited the greatest transcriptional activity at driving luciferase expression, but not significantly different from that of P1 (-2089/+7). The activity of P1, P2, and P3 (-1019/+7) was highly significantly higher than that of others (P〈0.01), suggesting that two positive regulatory elements were likely present in the regions of -1640/-1019 and -1019/-647. The results also showed that the -1640/ -647 region was indispensable for the promoter. The results of chromatin immunoprecipitation (CHIP) demonstrated that the NF-KB subunit p65 binds to one site around -15451-1531. Using four reference genes, we found that the over-expression of p65 enhanced the expression of CBR1 (P〈0.05) in porcine endometrial epithelial cells, while knockdown of the p65 did not down-regulate the CBRI expression. These results indicated that NF-KB (p65) could bind to the special element of CBR1 gene promoter in porcine endometrial epithelial cells in vitro. The binding site of NF-KB was a positive regulator for the CBR1 gene promoter, but was not necessary for the basic expression. 展开更多
关键词 PIG Carbonyl reductase 1 (CbR1) PROMOTER NF-Kb ENDOMETRIUM
原文传递
Therapeutic efficacy of moxibustion plus medicine in the treatment of infertility due to polycystic ovary syndrome and its effect on serum immune inflammatory factors 被引量:6
17
作者 Ye Tie-jun Cheng Hong-xia 《Journal of Acupuncture and Tuina Science》 CSCD 2020年第4期269-275,共7页
Objective:To explore the effects and related mechanism of heat-sensitive moxibustion plus clomifene citrate capsules for infertility due to polycystic ovary syndrome(PCOS).Methods:A total of 70 patients were randomize... Objective:To explore the effects and related mechanism of heat-sensitive moxibustion plus clomifene citrate capsules for infertility due to polycystic ovary syndrome(PCOS).Methods:A total of 70 patients were randomized into a control group and an observation group by the random number table method,with 35 cases in each group.The control group was treated with clomifene citrate capsules,while the observation group was treated with heat-sensitive moxibustion on the basis of the medications in the control group.The treatment course lasted for 6 menstrual cycles.The endometrial thickness and ovarian volume of the patients were measured before and after treatment.The levels of serum sex hormones,tumor necrosis factor-α(TNF-α)and nuclear factor-κB(NF-κB)were detected.Follow-up was performed for 1 year after treatment,and the pregnancy rate was recorded.Results:After treatment,the endometrium of the patients in both groups was significantly thickened,the ovarian volume was significantly reduced,and the intra-group differences were statistically significant(all P<0.05).The endometrium thickness and ovarian volume in the observation group were significantly different from those in the control group(both P<0.05).After treatment,the levels of serum testostrn(T)and luteinizing hormone(LH)in both groups decreased significantly,the level of estradiol(E2)increased obviously,and the intra-group differences were statistically significant(all P<0.05).The levels of serum T and LH in the observation group were lower than those in the control group,and the E2 level in the observation group was higher than that in the control group,and the between-group differences were statistically significant(all P<0.05).After treatment,the levels of serum TNF-αand NF-κB of the patients in both groups decreased,and the intra-group differences were statistically significant(all P<0.05).The levels of serum TNF-αand NF-κB in the observation group were significantly lower than those in the control group(both P<0.05).After 1-year follow-up,the pregnancy rate was 20.0%in the control group,versus 51.5%in the observation group.The pregnancy rate in the observation group was higher than that in the control group,and the difference was statistically significant(P<0.05).Conclusion:The treatment of heat-sensitive moxibustion plus clomifene citrate capsules can increase the endometrium thickness,reduce the ovarian volume,improve the status of serum sex hormone disorders,and increase the conception rate in patients with infertility due to PCOS,which may be related to the adjustment of the levels of immune inflammatory factors such as serum TNF-αand NF-κB. 展开更多
关键词 Moxibustion Therapy Heat-sensitive Moxibustion Polycystic Ovary Syndrome Gonadal Steroid Hormones Tumor Necrosis Factor-α NF-kappa b Infertility Female
原文传递
Toughness and [a,b]-factors with inclusion/exclusion properties
18
作者 WU ZeFang LIU GuiZhe YU QingLin 《Science China Mathematics》 SCIE 2011年第7期1491-1498,共8页
In this paper, we investigate the existence of [a,b]-factors with inclusion/exclusion properties under the toughness condition. We prove that if an incomplete graph G satisfies t(G) (a-1) + ab and a,b are two integers... In this paper, we investigate the existence of [a,b]-factors with inclusion/exclusion properties under the toughness condition. We prove that if an incomplete graph G satisfies t(G) (a-1) + ab and a,b are two integers with b > a > 1, then for any two given edges e1 and e2, there exist an [a,b]-factor including e1,e2; and an [a,b]-factor including e1 and excluding e2; as well as an [a,b]-factor excluding e1,e2 unless e1 and e2 have a common end in the case of a = 2. For complete graphs, we obtain a similar result. 展开更多
关键词 [a b]-factor inclusion/exclusion property TOUGHNESS
原文传递
Linkage with cathepsin B-sensitive dipeptide promotes the in vitro and in vivo anticancer activity of PEGylated tumor necrosis factor-alpha(TNF-α) against murine fibrosarcoma 被引量:4
19
作者 DAI ChuanYun FU Ya +4 位作者 LI Biao WANG YiGuang ZHANG Xuan WANG JianCheng ZHANG Qiang 《Science China(Life Sciences)》 SCIE CAS 2011年第2期128-138,共11页
To improve the pharmacological profile of tumor necrosis factor alpha(TNF-α),we have synthesized a new PEGylated prodrug,PEG-vcTNF-α,using a cathepsin B-sensitive dipeptide(valine-citrulline,vc) to link branched PEG... To improve the pharmacological profile of tumor necrosis factor alpha(TNF-α),we have synthesized a new PEGylated prodrug,PEG-vcTNF-α,using a cathepsin B-sensitive dipeptide(valine-citrulline,vc) to link branched PEG and TNF-α.PEG-modified TNF-α without the dipeptide linker(PEG-TNF-α) and unconjugated TNF-α were also tested as controls.It was found for the first time that TNF-α released from PEG-vcTNF-α was specifically dependent on the presence of cathepsin B.PEG-vcTNF-α induced higher cytotoxicity and greater apoptosis against L929 murine fibrosarcoma cells than PEG-TNF-α.Reversal of these effects by a cathepsin-B inhibitor confirmed that these effects were mediated by cathepsin B-specific release of TNF-α.In vivo pharmacokinetics studies demonstrated that the plasma stability of PEG-vcTNF-α was significantly increased compared to TNF-α.Finally,the improved anticancer efficacy of PEG-vcTNF-α and the distinct activities among the three formulations confirmed the positive contribution of both PEGylation and the dipeptide linkage to the improved drug-like properties of PEG-vcTNF-α.The results here indicate that linking proteins and PEG via the cathepsin B-sensitive dipeptide may be a promising strategy for developing protein therapeutics. 展开更多
关键词 tumor necrosis factor alpha PEGYLATION cathepsin b-sensitive dipeptide antitumor effect
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部