Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a ...Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.展开更多
目的:研究乙肝病毒X蛋白结合蛋白(hepatitis B X-interacting protein,HBXIP)对唾液腺腺样囊性癌肺高转移细胞株(ACC-M)增殖、迁移和侵袭的影响,及其对PI3K/Akt信号通路的影响。方法:将HBXIP质粒转染到ACC-M中,将细胞分为实验组(转染pEG...目的:研究乙肝病毒X蛋白结合蛋白(hepatitis B X-interacting protein,HBXIP)对唾液腺腺样囊性癌肺高转移细胞株(ACC-M)增殖、迁移和侵袭的影响,及其对PI3K/Akt信号通路的影响。方法:将HBXIP质粒转染到ACC-M中,将细胞分为实验组(转染pEGFP-N1-HBXIP质粒)、对照组1(未转染组)和对照组2(vector组,pEGFP-N1)。采用RT-PCR检测HBXIP在ACC-M中的表达;采用MTT实验、Transwell小室实验和划痕实验检测HBXIP过表达对ACC-M的增殖、迁移和侵袭的影响;Western印迹法检测HBXIP过表达对Akt、p-Akt、PI3K、p-PI3K及S100A4蛋白表达量的影响。采用SPSS18.0软件包对数据进行统计学分析。结果:MTT实验结果显示,实验组中存活的细胞数显著高于对照组(P<0.05);划痕实验结果显示,实验组细胞迁移率显著高于对照组(P<0.01);Transwell小室实验结果显示,实验组细胞侵袭个数显著高于对照组(P<0.01);Western印迹法结果显示,相对于对照组,实验组随着HBXIP过表达,p-Akt、p-PI3K及S100A4表达量相对增高。结论:HBXIP基因过表达ACC-M增殖、迁移和侵袭具有影响,可能通过促进Akt、PI3K磷酸化及S100A4蛋白表达量增加,促进ACC-M的增殖、迁移和侵袭。展开更多
Several kinds of stream ciphers—complementary sequences of period sequences,partial sum of period sequences,inverse order sequences and finitely generated sequences,arestudied by using techniques of generating functi...Several kinds of stream ciphers—complementary sequences of period sequences,partial sum of period sequences,inverse order sequences and finitely generated sequences,arestudied by using techniques of generating functions.Their minimal polynomials,periods,as wellas generating functions are given.As to finitely generated sequences,the change of their linearcomplexity profiles as well as the relationship between the two generated sequences usder thecase in which the degree of connected polynomials are fixed,are discussed.展开更多
The discovery of a data based informational wave pattern infuses coherent entanglement into the system. This discovery of how to add coherent entanglement into the model provides the missing key, that has opened the d...The discovery of a data based informational wave pattern infuses coherent entanglement into the system. This discovery of how to add coherent entanglement into the model provides the missing key, that has opened the door to understanding the universe. It is found that the inclusion of coherence and entanglement at the start of the system is extremely simple and in fact it is so simple that it has simply been overlooked. Entanglement and coherence are the most fundamental aspects of our universe. It is demonstrated that the basic model of the hydrogen atom is made from the CMB. If we add entanglement into this basic model of the hydrogen atom a math system called Wave Pattern Entangled Math is unveiled. This system of wave interference mathematics creates a data system in which entanglement and coherence can easily be understood. The final outcome is an unbreakable pattern of information, including entangled energy, entropy, spin, universal expansion, compression, velocity of light, C2, and Quantum Coherence.展开更多
This paper is an eye opening to the new horizon of the design of operational Compressors in our jet engines. That are compressors usually perform an operation called isentropic process and which levitate the pressure ...This paper is an eye opening to the new horizon of the design of operational Compressors in our jet engines. That are compressors usually perform an operation called isentropic process and which levitate the pressure and temperature to the optimum level which require for effective ignition. Basically, our compressors have several sets of blades to perform this function, more precisely saying Rotor and stator blades. Where rotor blade provides air molecule to push at very high velocity to the Stationary blade and when the air Enders to the Stator, the stator races its pressure to move on to the next stage. And we call this set of Stator and rotor as a stage ref [1]. However, in this work, I consider the geometry of the incoming air molecule and how it transforms its physical quantities such as Pressure and temperature ref [2]. For that I tie the concept of Thermodynamic and mechanics on the platform of Tensor analysis ref [3]. That is, I consider the quantities like Pressure, Temperature and rate of flow are their corresponding vector spaces and energy related quintets like heat, work as the scaling elements on the above vector space. And quantities such as entropy enthalpy and specific heat capacity are corresponding physics of it. Considering the advantages, one of the important advantages of this approach is the applicability of results of this work to the formulation of blade less compression Example: Ram and Scram jet engine. Again, the relevant upgrading which is essential for future hypersonic air crafts can achieve from this study and this will be a mile stone for bright air and space travel. To conclude, this approach will be a great transformation on the conventional idea for realization of compression for operational Scram and Ram jet engines ref [4] [5].展开更多
For partial linear model Y = X~τβ_0 + g_0(T) + ε with unknown β_0 ∈ R^dand an unknown smooth function g_0, this paper considers the Huber-Dutter estimators of β_0, scaleσ for the errors and the function g_0 res...For partial linear model Y = X~τβ_0 + g_0(T) + ε with unknown β_0 ∈ R^dand an unknown smooth function g_0, this paper considers the Huber-Dutter estimators of β_0, scaleσ for the errors and the function g_0 respectively, in which the smoothing B-spline function isused. Under some regular conditions, it is shown that the Huber-Dutter estimators of β_0 and σ areasymptotically normal with convergence rate n^(-1/2) and the B-spline Huber-Dutter estimator of g_0achieves the optimal convergence rate in nonparametric regression. A simulation study demonstratesthat the Huber-Dutter estimator of β_0 is competitive with its M-estimator without scale parameterand the ordinary least square estimator. An example is presented after the simulation study.展开更多
Consider the partially linear modelY<sub>i</sub>=X<sub>i</sub><sup>τ</sup>β<sub>0</sub>+ g<sub>0</sub>（T<sub>i</sub>）+e<sub>i</sub&g...Consider the partially linear modelY<sub>i</sub>=X<sub>i</sub><sup>τ</sup>β<sub>0</sub>+ g<sub>0</sub>（T<sub>i</sub>）+e<sub>i</sub>,where （T<sub>1</sub>, X<sub>1</sub><sup>τ</sup>, Y<sub>1</sub>）,…,（T<sub>n</sub>,X<sub>n</sub><sup>τ</sup>, Y<sub>n</sub>） are i. i. d. observations of a random （d+2）-vector （T,X<sup>τ</sup>, Y）, X=（x<sub>1</sub>,…,x<sub>d</sub>）<sup>τ</sup> is a d-vector of explanatory variables, β<sub>0</sub> is a vector of unknownparameters, T is another explanatory variable ranging over a nondegenerate compactinterval, say [0, 1],g<sub>0</sub>（·）is an unknown smooth function, and e<sub>i</sub>’s are random errors.展开更多
基金supported by grants from the National Natural Science Foundation of China, Nos. 81930031 (to JNZ), 81720108015 (to JNZ), 81901525 (to SZ), 82101440 (to DDS), 81801234 (to YZ) and 82071389 (to GLY)the Natural Science Foundation of Tianjin, Nos. 20JCQNJC01270 (to JWW), 20JCQNJC00460 (to GLY), 18JCQNJC81000 (to HTR)+4 种基金Scientific Research Project of Tianjin Education Commission (Natural Science), No. 2018KJ052 (to ZWZ)Tianjin Health and Health Committee Science and Technology Project, No. QN20015 (to JWW)the Science & Technology Development Fund of Tianjin Education Commission for Higher Education, No. 2016YD02 (to YW)Tianjin Key Science and Technology Projects of Innovative Drugs and Medical Devices, No. 19ZXYXSY00070 (to YW)the Clinical Research Fundation of Tianjin Medical University, No. 2018kylc002 (to YW)
文摘Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.
文摘目的:研究乙肝病毒X蛋白结合蛋白(hepatitis B X-interacting protein,HBXIP)对唾液腺腺样囊性癌肺高转移细胞株(ACC-M)增殖、迁移和侵袭的影响,及其对PI3K/Akt信号通路的影响。方法:将HBXIP质粒转染到ACC-M中,将细胞分为实验组(转染pEGFP-N1-HBXIP质粒)、对照组1(未转染组)和对照组2(vector组,pEGFP-N1)。采用RT-PCR检测HBXIP在ACC-M中的表达;采用MTT实验、Transwell小室实验和划痕实验检测HBXIP过表达对ACC-M的增殖、迁移和侵袭的影响;Western印迹法检测HBXIP过表达对Akt、p-Akt、PI3K、p-PI3K及S100A4蛋白表达量的影响。采用SPSS18.0软件包对数据进行统计学分析。结果:MTT实验结果显示,实验组中存活的细胞数显著高于对照组(P<0.05);划痕实验结果显示,实验组细胞迁移率显著高于对照组(P<0.01);Transwell小室实验结果显示,实验组细胞侵袭个数显著高于对照组(P<0.01);Western印迹法结果显示,相对于对照组,实验组随着HBXIP过表达,p-Akt、p-PI3K及S100A4表达量相对增高。结论:HBXIP基因过表达ACC-M增殖、迁移和侵袭具有影响,可能通过促进Akt、PI3K磷酸化及S100A4蛋白表达量增加,促进ACC-M的增殖、迁移和侵袭。
文摘Several kinds of stream ciphers—complementary sequences of period sequences,partial sum of period sequences,inverse order sequences and finitely generated sequences,arestudied by using techniques of generating functions.Their minimal polynomials,periods,as wellas generating functions are given.As to finitely generated sequences,the change of their linearcomplexity profiles as well as the relationship between the two generated sequences usder thecase in which the degree of connected polynomials are fixed,are discussed.
文摘The discovery of a data based informational wave pattern infuses coherent entanglement into the system. This discovery of how to add coherent entanglement into the model provides the missing key, that has opened the door to understanding the universe. It is found that the inclusion of coherence and entanglement at the start of the system is extremely simple and in fact it is so simple that it has simply been overlooked. Entanglement and coherence are the most fundamental aspects of our universe. It is demonstrated that the basic model of the hydrogen atom is made from the CMB. If we add entanglement into this basic model of the hydrogen atom a math system called Wave Pattern Entangled Math is unveiled. This system of wave interference mathematics creates a data system in which entanglement and coherence can easily be understood. The final outcome is an unbreakable pattern of information, including entangled energy, entropy, spin, universal expansion, compression, velocity of light, C2, and Quantum Coherence.
文摘This paper is an eye opening to the new horizon of the design of operational Compressors in our jet engines. That are compressors usually perform an operation called isentropic process and which levitate the pressure and temperature to the optimum level which require for effective ignition. Basically, our compressors have several sets of blades to perform this function, more precisely saying Rotor and stator blades. Where rotor blade provides air molecule to push at very high velocity to the Stationary blade and when the air Enders to the Stator, the stator races its pressure to move on to the next stage. And we call this set of Stator and rotor as a stage ref [1]. However, in this work, I consider the geometry of the incoming air molecule and how it transforms its physical quantities such as Pressure and temperature ref [2]. For that I tie the concept of Thermodynamic and mechanics on the platform of Tensor analysis ref [3]. That is, I consider the quantities like Pressure, Temperature and rate of flow are their corresponding vector spaces and energy related quintets like heat, work as the scaling elements on the above vector space. And quantities such as entropy enthalpy and specific heat capacity are corresponding physics of it. Considering the advantages, one of the important advantages of this approach is the applicability of results of this work to the formulation of blade less compression Example: Ram and Scram jet engine. Again, the relevant upgrading which is essential for future hypersonic air crafts can achieve from this study and this will be a mile stone for bright air and space travel. To conclude, this approach will be a great transformation on the conventional idea for realization of compression for operational Scram and Ram jet engines ref [4] [5].
基金Supported by The National Natural Science Foundation of China (No. 10231030 )Beijing Normal University Youth Foundation (No. 104951).
文摘For partial linear model Y = X~τβ_0 + g_0(T) + ε with unknown β_0 ∈ R^dand an unknown smooth function g_0, this paper considers the Huber-Dutter estimators of β_0, scaleσ for the errors and the function g_0 respectively, in which the smoothing B-spline function isused. Under some regular conditions, it is shown that the Huber-Dutter estimators of β_0 and σ areasymptotically normal with convergence rate n^(-1/2) and the B-spline Huber-Dutter estimator of g_0achieves the optimal convergence rate in nonparametric regression. A simulation study demonstratesthat the Huber-Dutter estimator of β_0 is competitive with its M-estimator without scale parameterand the ordinary least square estimator. An example is presented after the simulation study.
文摘Consider the partially linear modelY<sub>i</sub>=X<sub>i</sub><sup>τ</sup>β<sub>0</sub>+ g<sub>0</sub>（T<sub>i</sub>）+e<sub>i</sub>,where （T<sub>1</sub>, X<sub>1</sub><sup>τ</sup>, Y<sub>1</sub>）,…,（T<sub>n</sub>,X<sub>n</sub><sup>τ</sup>, Y<sub>n</sub>） are i. i. d. observations of a random （d+2）-vector （T,X<sup>τ</sup>, Y）, X=（x<sub>1</sub>,…,x<sub>d</sub>）<sup>τ</sup> is a d-vector of explanatory variables, β<sub>0</sub> is a vector of unknownparameters, T is another explanatory variable ranging over a nondegenerate compactinterval, say [0, 1],g<sub>0</sub>（·）is an unknown smooth function, and e<sub>i</sub>’s are random errors.
