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An E. coli SOS-EGFP biosensor for fast and sensitive detection of DNA damaging agents 被引量:2
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作者 Zhilan Chen Meiling Lu +1 位作者 Dandan Zou Hailin Wang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2012年第3期541-549,共9页
An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation... An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals. The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor, and then the reporter egfp gene under the control of recA promoter is stimulated to express as a fluorescent protein, allowing fast and sensitive fluorescence detection. Interestingly, this biosensor can be simultaneously applied for evaluation of genotoxicity and cytotoxicity. The SOS-EGFP bacterial biosensor provides a sensitive, specific and simple method for detecting known and potential DNA damaging chemicals. 展开更多
关键词 bacterial biosensor detection DNA damage GENOTOXINS SOS response
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