A Study of Bacterial Linear Growth Using A Microcalorimetric Method

By using an LKB-2277 bioactivity monitor, we have determined the thermogenesis curves of four kinds of anaerobes linear growth. From the thermogenesis curves, we established the growth thermokinetic equation:dp/dt = km Pn,the order of growth metabolism n=0, and calculated the mean specific rate constant of multiplication μ. It is a new kind of bacterial growth,it was compared with exponential growth.

细胞动力学研究──Ⅱ.产物抑制生长过程的热动力学

By using an LKB2277 Bioactivity Monitor,we have detedrined the thermogenesis power cu-rves of four kinds of Brucellas S2(55007), M5 (55009),83-202,83-980. And established the thermokinetic equation for the process of cell growth inlilbited by the products of metabolism, as:From this equation, the rate constants of cell growth k [= k0(1 - β’ P0)] were obtained.This thermokinetic equation is very suitable fdr cell growth of separated culture and is very important for the study of bacterial limited growth and their characteristics.

细胞动力学研究──Ⅲ.细菌算术级数式生长过程热动力学

By using an LKB-2277 Bioactivity Monitor, we have deterthened the thermogenesis curves of two species of Brucellas arithmetic series growth. nom the thermogenesis curves, a thermokinetic equation as:could be established for bacterial arithmetic series growth in which the order of growth metabolism n=0. The mean specific rate constant of multiplication and the mean generation time G et al. were calculated. This model was compared with the exponentialmodel.

细菌有限生长热动力学研究

The thermograms of Brucalles Br.10 and Br-981 have been deterAnned. From the thermograJns, a thermokinetic equation ln =lnb - kt could be established for the liAnted growth of bacteria. From this equation, the growth constant k, the intial growth rate 6, the mean specific growth rate k/m, and the mean thermal power increasing rate Pmaxk/(2m + 2) were calculated. This equation is very significant for the study of bacteriaJ growth and their characteristics.

细胞动力学研究──Ⅳ.细菌非理想生长过程的热动力学

By using LKB2277 Bioactivity Monitor, we have determined the thermogenesis power curves of four kinds of bacteria: Brucella M5(55010), 83-980, 83-981 and E. coli. We have also derived the thermokinetic equation for the process of bacterial growth, which was untypical "S" , unideal growth, as:From this equation, the rate constants of bacterial growth k wore botained.This thermokinetic equation is very suitable for cell growth of separated culture, in which the thermogenesis curve is untypical. The model propoted by equationl(1) has been compared with the exponential and logistic models. This thermokinetic equation is very significant for the study of bacterial limited growth and their characteristics.

大鼠睾丸支持细胞和间质细胞分离和培养方法的研究

大鼠睾丸支持细胞和间质细胞分离和培养方法的研究金向阳，李潭北京医科大学生理系（北京１０００８３）；高鹏徐州矿物局职工医院；常元勋，崔京伟，江泉观北京医科大学劳动卫生学教研室国家自然科学基金资助（３９１７０６６３）整体动物实验是评价化学物质性腺毒性的一...

细菌变异株生长热谱研究

用微量热法研究微生物的生长与代谢,是生物热力学一个新的研究领域.我们已经测得细菌生长的完整热谱,这种热谱具有良好的重现性和明显的特征性,可以作为细菌的“指纹图”对其进行种的分类和鉴定[1～5].但在研究中发现,同种细菌不同变异株的生长图谱略有差异,若培养基选择?..

MnMoO_4·H_2O纳米棒生长过程的原位热动力学研究

在298.15 K下采用现代微量热技术监测了微乳液法原位合成MnMoO4.H2O纳米棒过程中能量变化的微量热曲线.该曲线显示,反应开始瞬时放热,有一个尖锐的放热峰,在随后的过程中分别出现一个强的吸热峰和放热峰.通过X射线粉末衍射仪(XRD)、场发射扫描电子显微镜(FESEM)和透射电子显微镜(TEM)等对MnMoO.4H2O纳米棒的结构、形貌及尺寸进行了表征.结合微乳液的特性和量热曲线,讨论了MnMoO4.H2O纳米棒生长过程中的形貌演变和热动力学信息.整个生长过程包含微乳液的碰撞凝聚、反应成核、结晶和生长过程.经计算,反应成核过程、晶化过程及晶体二次生长过程的速率常数分别为6.35×10-3,7.18×10-4和9.16×10-5s-1.生长速率小于成核速率,这有利于纳米材料的形成.

微生物代谢特征热化学研究进展

对生物体系新陈代谢规律的研究和探讨是生命科学研究的重要内容之一.用热力学和动力学原理及微量热技术研究微生物的代谢特征,建立微生物体系在各种不同条件下的热动力学模型,寻求其生长代谢规律,已成为一项新兴的有发展前途的研究课题.本文着重介绍微生物代谢特征热化学的研究进展.

细胞动力学研究Ⅴ.细胞连续增长热动力学

用微量热法测定了布鲁氏菌Ｍ５（５５０１０）和８３－９８１在２７℃和４０℃时的生长发热功率曲线．由这些生长发热功率曲线，建立了细胞有限条件下连续增长热动力学方程：ｌｎ（Ｐｍ／Ｐｔ－１）＝ｌｎ（Ｐｍ／Ｐ０－１）－ｋｔ从而求出了细菌的生长常数ｋ．这个方程非常适合“Ｓ”

Detection and Response Mechanism of CN^- Using Ag_2S Coated Piezoelectric Crystal Sensor

By using LKB2277 bioactivity monitor, we have studied the effect of Na 2SeO 3 on Bacillus subtilis Growth at two different media and found the antagonism of protein on its toxicity. Low concentration of Na 2SeO 3 has promoting action, and high concentration of Na 2SeO 3 has inhibitory action. Half inhibitory concentration ( IC 50 ) of BPY medium is 4.2×10 -1 g / L, and IC 50 of PD medium is 2.2×10 -2 g / L.

硫化镉纳米管的制备及其原位生长的热力学研究

为获取纳米材料生长过程的原位热力学瞬时信息,以实现纳米材料优化设计,文中以硫化镉(Cd S)纳米管为目标材料,在298.15 K下采用高精度、高灵敏度的现代微热量技术获取其生长过程的原位热谱曲线。结果显示,反应开始瞬时吸热,并有1个尖锐的吸热峰,在随后的过程中连续出现3个放热峰。为探讨Cd S纳米管生长过程的形貌演变、热力学信息及生长机理,结合X射线衍射粉末仪(XRD)、场发射扫描电子显微镜(FE-SEM)和透射电子显微镜(TEM)、荧光光度计(PL)等对Cd S纳米管的结构、形貌、尺寸及光学性能进行表征,结果表明,整个生长过程先吸热后放热,依次经历微乳液混合、晶体成核、液滴重组和晶体生长。研究结果可揭示纳米材料生长过程的热力学特征,丰富纳米材料非平衡生长过程的科学内涵,发展纳米物理化学的理论方法。

细菌代谢特征的热化学研究概况

用热力学、动力学原理及微量热技术研究细菌的代谢特征和代谢规律，是促进生物学学科发展和进行生命科学研究的重要内容之一，对此作者着重介绍了细菌代谢特征的热化学方法研究的概况。

基于微量量热法的连花清瘟胶囊质量一致性评价方法的建立

目的考察连花清瘟胶囊(LQC)对绿脓杆菌Pseudomonas aeruginosa生长代谢的影响,建立评价LQC质量一致性的新方法。方法采用微量量热法测定不同质量浓度LQC对绿脓杆菌的生长代谢的影响,得到热谱曲线及相应的热动力学参数,如生长峰达峰时间(T)、生长期发热量(Q)、生长期最大发热功率(P)、生长期生长速率常数(k)等。以LQC质量浓度(C)为横坐标,T、P、Q、k为纵坐标进行回归分析,根据相关系数优选最佳参数,计算抑菌有效率(antibacterial effective rate,E)。以E作为评价LQC抗菌活性指标,分别检测11个批次市售LQC以及经特殊处理后的LQC样品对绿脓杆菌生长代谢的影响。结果 LQC质量浓度在10.00~47.68 mg/m L时对绿脓杆菌生长代谢具有明显的抑制作用,且具有质量浓度依赖性。通过回归分析得出的4个热动力学参数中,相关性最好的参数为T,r=0.990,其变化可以直观评价LQC对绿脓杆菌生长代谢的影响,且该参数稳定性较好,故选择指数生长期的T计算E。结果表明不同批次LQC样品E无明显差异,样品放于潮湿或高温环境中其E发生明显变化。结论该方法具有灵敏度高、重现性好的特点,可以区分不同质量LQC的抗菌生物活性,为LQC质量的一致性评价提供了一种参考方法,并为其他大复方中药制剂质量评价提供了参考依据和方法学借鉴。