[Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing(AEE)and its microemulsion(AEE-ME).[Method]The acute toxicity was tested in mice by intragastric administration,and median l...[Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing(AEE)and its microemulsion(AEE-ME).[Method]The acute toxicity was tested in mice by intragastric administration,and median lethal dose(LD50)as well as its 95% confidence interval was calculated by modified Karber method;the bacteriostasis was investigated by cylinder plate method.[Result]LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg;and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4.388-6.566 g/kg.The MICs of AEE to Escherichia coli O149 from swine,Staphylococcus aureus,Salmonella pullorum and Streptococcus agalactiae were 10.00,20.00,20.00 and 10.00 mg/ml,respectively;while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00,10.00,5.00 and 5.00 mg/ml in turn,and that to Pseudomonas aeruginosa is 20.00 mg/ml.[Conclusion]AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation.AEE and AEE-ME have obvious bacteriostasis,in which AEE-ME is superior to AEE.展开更多
[ Objective ] The aim was to study bacteriostatic activity of the different extract from Ilex Kudingcha C. J. Tseng. [ Method ] By using test tube 2 -fold dilution method and Kirby-Baueer Disc Diffusionto,we conducted...[ Objective ] The aim was to study bacteriostatic activity of the different extract from Ilex Kudingcha C. J. Tseng. [ Method ] By using test tube 2 -fold dilution method and Kirby-Baueer Disc Diffusionto,we conducted inhibitory test on S. aureus and E, coil and determined the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC）and the diameter of inhibition zone. [ Result ] The extract 1 had fairly strong in vitro bacteriostasis activities and than the extract2 and extract3. The MIC and MBC was 3.91 mg/ml and 31.25 mg/ml on S. aureus and E. coil [ Conclusion] The result showed that the different extract from Ilex Kudingcha C. J. Tseng had varying amount of different bacteriostatic activities,the Ilex Kudingcha C. J. Tseng was developed new and safe bacteriostatic reagent to provide reference.展开更多
Nano selenium which has the advantages of low toxicity,strong activity and high biocompatibility has broad-spectrum antibacterial activity to bacteria,fungi,viruses and parasites,and has a broad application prospect i...Nano selenium which has the advantages of low toxicity,strong activity and high biocompatibility has broad-spectrum antibacterial activity to bacteria,fungi,viruses and parasites,and has a broad application prospect in the field of inhibiting microbial infection.This paper mainly reviews the progress in the types and mechanisms of selenium inhibition microorganisms,and prospects the development of the antibacterial activities of selenium nanoparticles.展开更多
[Objective] The paper was to explore the combined inhibitory effect of rhizoma coptidis-folium isatidis and rhizoma coptidis-flos poprli against Escherichia coli O2.[Method] Contrast test of single and associated bact...[Objective] The paper was to explore the combined inhibitory effect of rhizoma coptidis-folium isatidis and rhizoma coptidis-flos poprli against Escherichia coli O2.[Method] Contrast test of single and associated bacteriostasis against known serotype E. coli O2 was conducted using microcheckerboard method.[Result] The MIC of rhizoma coptidis, folium isatidis and flos poprli were 1/8 extracting liquid, 1/8 extracting liquid and 1/2 extracting liquid, respectively. When combined with folium isatidis or flos poprli, the MIC of rhizoma coptidis was 1/8 extracting liq-uid or 1/16 extracting liquid compared with single use. When combined with rhizoma coptidis, the MIC of folium isatidis and flos poprli were 1/8 extracting liquid and 1/16 extracting liquid.[Conclusion] When rhizoma coptidis was combined with folium isatidis or flos poprli, the FIC values were 2 and 0.625, performing independent action and additive effect, respectively.展开更多
基金Supported by Specific Program Funded by Basic Scientific Research Operating Expenses of Central Public Scientific Research Institutes(BRF060403)Key Project of Scientific and Technical Supporting Pro-grams of Gansu Province(0804NKCA074)~~
文摘[Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing(AEE)and its microemulsion(AEE-ME).[Method]The acute toxicity was tested in mice by intragastric administration,and median lethal dose(LD50)as well as its 95% confidence interval was calculated by modified Karber method;the bacteriostasis was investigated by cylinder plate method.[Result]LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg;and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4.388-6.566 g/kg.The MICs of AEE to Escherichia coli O149 from swine,Staphylococcus aureus,Salmonella pullorum and Streptococcus agalactiae were 10.00,20.00,20.00 and 10.00 mg/ml,respectively;while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00,10.00,5.00 and 5.00 mg/ml in turn,and that to Pseudomonas aeruginosa is 20.00 mg/ml.[Conclusion]AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation.AEE and AEE-ME have obvious bacteriostasis,in which AEE-ME is superior to AEE.
基金Supported by the Key Project in Natural Science Foundation ofGuangxi Province(0719004-3H)~~
文摘[ Objective ] The aim was to study bacteriostatic activity of the different extract from Ilex Kudingcha C. J. Tseng. [ Method ] By using test tube 2 -fold dilution method and Kirby-Baueer Disc Diffusionto,we conducted inhibitory test on S. aureus and E, coil and determined the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC）and the diameter of inhibition zone. [ Result ] The extract 1 had fairly strong in vitro bacteriostasis activities and than the extract2 and extract3. The MIC and MBC was 3.91 mg/ml and 31.25 mg/ml on S. aureus and E. coil [ Conclusion] The result showed that the different extract from Ilex Kudingcha C. J. Tseng had varying amount of different bacteriostatic activities,the Ilex Kudingcha C. J. Tseng was developed new and safe bacteriostatic reagent to provide reference.
基金Special Fund for Technological Innovation Guidance of Shaanxi Provincial Department of Science and Technology(2017CG-003)Key R&D Projects in Shaanxi Province(2018NY-043).
文摘Nano selenium which has the advantages of low toxicity,strong activity and high biocompatibility has broad-spectrum antibacterial activity to bacteria,fungi,viruses and parasites,and has a broad application prospect in the field of inhibiting microbial infection.This paper mainly reviews the progress in the types and mechanisms of selenium inhibition microorganisms,and prospects the development of the antibacterial activities of selenium nanoparticles.
基金Supported by Natural Science Foundation of Shandong Province(ZR2014CQ012)
文摘[Objective] The paper was to explore the combined inhibitory effect of rhizoma coptidis-folium isatidis and rhizoma coptidis-flos poprli against Escherichia coli O2.[Method] Contrast test of single and associated bacteriostasis against known serotype E. coli O2 was conducted using microcheckerboard method.[Result] The MIC of rhizoma coptidis, folium isatidis and flos poprli were 1/8 extracting liquid, 1/8 extracting liquid and 1/2 extracting liquid, respectively. When combined with folium isatidis or flos poprli, the MIC of rhizoma coptidis was 1/8 extracting liq-uid or 1/16 extracting liquid compared with single use. When combined with rhizoma coptidis, the MIC of folium isatidis and flos poprli were 1/8 extracting liquid and 1/16 extracting liquid.[Conclusion] When rhizoma coptidis was combined with folium isatidis or flos poprli, the FIC values were 2 and 0.625, performing independent action and additive effect, respectively.
