The proliferation, differentiation and fibrosis of lens epithelia cells （LECs） is mainly responsible for posterior capsular opacification （PCO）. From the primary culture of LECs to the culture of lens capsular bag...The proliferation, differentiation and fibrosis of lens epithelia cells （LECs） is mainly responsible for posterior capsular opacification （PCO）. From the primary culture of LECs to the culture of lens capsular bag, the models of posterior capsular opacification have been developed. At present, the most commonly used model is cell culture in medium with serum. But the culture in pure ocular tissue has not been reported. Therefore, we established a new model of posterior capsular opacification-culturing bovine lens capsular bag in pure ocular tissue to exclude the role of serum. Our study established a new culture method to investigate the proliferation, differentiation and apoptosis of lens epithelia cells in the aqueous humor with or without lens cortex and vitreous humor. The purpose of the study is to model posterior capsular opacification in vivo as closely as possible and to discuss the influence of ocular tissue on posterior capsular opacification.展开更多
基金This study was supported by grants from the Foundation for Youth Pioneer Tutor of Henan Province (No. 2001225 and 2003100) and the Foundation for Technical Creative Talent Program of Henan Province (No. 20008).
文摘The proliferation, differentiation and fibrosis of lens epithelia cells （LECs） is mainly responsible for posterior capsular opacification （PCO）. From the primary culture of LECs to the culture of lens capsular bag, the models of posterior capsular opacification have been developed. At present, the most commonly used model is cell culture in medium with serum. But the culture in pure ocular tissue has not been reported. Therefore, we established a new model of posterior capsular opacification-culturing bovine lens capsular bag in pure ocular tissue to exclude the role of serum. Our study established a new culture method to investigate the proliferation, differentiation and apoptosis of lens epithelia cells in the aqueous humor with or without lens cortex and vitreous humor. The purpose of the study is to model posterior capsular opacification in vivo as closely as possible and to discuss the influence of ocular tissue on posterior capsular opacification.
