By the addition of rice wine koji, enhancement of the reactivity was observed for the baker's yeast reduction of β-keto esters into (S)-β-hydroxy esters with high enantiomeric purity (73-98%).
Baker’s yeast number 6 was selected by screening. It showed good catalytic activity and enantioselec-tivity for asymmetric reduction of 2,5-hexanedione to produce (2S,5S)-2,5-hexanediol. Gas chromatography-mass spect...Baker’s yeast number 6 was selected by screening. It showed good catalytic activity and enantioselec-tivity for asymmetric reduction of 2,5-hexanedione to produce (2S,5S)-2,5-hexanediol. Gas chromatography-mass spectrometry (GC-MS) revealed that the intermediate was (S)-5-hydroxyhexane-2-one. Reduction of 2,5-hexanedione proceeded in a two-step reaction. The hydroxyketone was initially formed, and this intermediate was further re-duced to the diol. Factors influencing the product yield and the enantiomeric excess of the reduction of 2,5-hexandione catalyzed by baker’s yeast number 6 were investigated. Higher concentration (≤100 mmol·L-1) of 2,5-hexandione did not influence 5-hydroxyhexane-2-one production, but 2,5-hexanediol production was inhibited by excess accumulation (>30 mmol·L-1) of intermediate. The optimal conditions were glucose as the co-substrate at an initial glucose concentration of 20 g·L-1, 34°C, pH 7.0 and cell concentration 60 g·L-1 (cell dry mass). Under the optimal condition and an initial substrate concentration of 30 mmol·L-1, the yield of 2,5-hexandiol was 78.7% and the enantiomeric excess of (2S,5S)-2,5-hexandiol was 94.4% for 24-h reduction.展开更多
The anatase nano-TiO2 powder, with crystal size between 40 and 80 nm, was prepared by the liquid phase hydrolysis of TiCl4. At the same time, the nano-TiO2 was utilized with the baker’s yeast biomass as a composite a...The anatase nano-TiO2 powder, with crystal size between 40 and 80 nm, was prepared by the liquid phase hydrolysis of TiCl4. At the same time, the nano-TiO2 was utilized with the baker’s yeast biomass as a composite adsorbent to adsorb the Cu ions in the artificial aqueous solution. The investigation showed that the composite adsorbent had a fine adsorption efficiency. The TiO2 in the composite ad- sorbent could cooperate well with baker’s yeast to improve the adsorbing capability of Cu2+ under the following experimental conditions as well: a quantity of composite adsorbent of 5 g·L?1, pH≥4.0, an adsorption time of 40 min and an initial concentration of Cu ions of 10 mg·L?1. In addition, the results of measurements, obtained with a scanning electron microscope, an infrared spectrophotometer and a Zeta potential analyzer, revealed that the baker’s yeast and nano-TiO2 produced the composite ad- sorbent through coordination and hydrogen bonds in particular, etc. The stability of the composite adsorbent and the amount of titania loaded were largely dependent on the concentration of hydrogen ion in the solution.展开更多
The fed-batch method for baker’s yeast culture is fundamental for compressed and activedry yeast making. As a technical know-how it has not been published hitherto. The pub-lication of our own fed-batch regime, in pr...The fed-batch method for baker’s yeast culture is fundamental for compressed and activedry yeast making. As a technical know-how it has not been published hitherto. The pub-lication of our own fed-batch regime, in principle and in practice, may serve the biologistsas well as engineers to get better results in microbial production.展开更多
文摘By the addition of rice wine koji, enhancement of the reactivity was observed for the baker's yeast reduction of β-keto esters into (S)-β-hydroxy esters with high enantiomeric purity (73-98%).
基金Supported by the Key Project of Science and Technology of Fujian Province (2008N0120)the Key Discipline of Biochemical Engineering of Fujian Province (Huaqiao University)
文摘Baker’s yeast number 6 was selected by screening. It showed good catalytic activity and enantioselec-tivity for asymmetric reduction of 2,5-hexanedione to produce (2S,5S)-2,5-hexanediol. Gas chromatography-mass spectrometry (GC-MS) revealed that the intermediate was (S)-5-hydroxyhexane-2-one. Reduction of 2,5-hexanedione proceeded in a two-step reaction. The hydroxyketone was initially formed, and this intermediate was further re-duced to the diol. Factors influencing the product yield and the enantiomeric excess of the reduction of 2,5-hexandione catalyzed by baker’s yeast number 6 were investigated. Higher concentration (≤100 mmol·L-1) of 2,5-hexandione did not influence 5-hydroxyhexane-2-one production, but 2,5-hexanediol production was inhibited by excess accumulation (>30 mmol·L-1) of intermediate. The optimal conditions were glucose as the co-substrate at an initial glucose concentration of 20 g·L-1, 34°C, pH 7.0 and cell concentration 60 g·L-1 (cell dry mass). Under the optimal condition and an initial substrate concentration of 30 mmol·L-1, the yield of 2,5-hexandiol was 78.7% and the enantiomeric excess of (2S,5S)-2,5-hexandiol was 94.4% for 24-h reduction.
基金Supported by the National Natural Science Foundation of China (Grant No. 30671530)the Key Basic Research Program of the Sichan Provincial Education Commission (Grant No. 2005A014)
文摘The anatase nano-TiO2 powder, with crystal size between 40 and 80 nm, was prepared by the liquid phase hydrolysis of TiCl4. At the same time, the nano-TiO2 was utilized with the baker’s yeast biomass as a composite adsorbent to adsorb the Cu ions in the artificial aqueous solution. The investigation showed that the composite adsorbent had a fine adsorption efficiency. The TiO2 in the composite ad- sorbent could cooperate well with baker’s yeast to improve the adsorbing capability of Cu2+ under the following experimental conditions as well: a quantity of composite adsorbent of 5 g·L?1, pH≥4.0, an adsorption time of 40 min and an initial concentration of Cu ions of 10 mg·L?1. In addition, the results of measurements, obtained with a scanning electron microscope, an infrared spectrophotometer and a Zeta potential analyzer, revealed that the baker’s yeast and nano-TiO2 produced the composite ad- sorbent through coordination and hydrogen bonds in particular, etc. The stability of the composite adsorbent and the amount of titania loaded were largely dependent on the concentration of hydrogen ion in the solution.
基金Project supported by the National Natural Science Foundation of China.
文摘The fed-batch method for baker’s yeast culture is fundamental for compressed and activedry yeast making. As a technical know-how it has not been published hitherto. The pub-lication of our own fed-batch regime, in principle and in practice, may serve the biologistsas well as engineers to get better results in microbial production.