The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear （Momordica charantia L.） were invest...The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear （Momordica charantia L.） were investigated. Sterilized for 6 rain with 0.1% （w/v） mercuric chloride solution is an essential sterilization method for stems. June and July proved to be better for material taking, followed by May. The callus formation rate of balsam pears reached the highest when medium added with IBA 1.0mg/L and BA 2.0 mg/L, while the optimum medium for adventitious bud induction was MS added with IBA 0.5mg/L and BA 4.0 mg/L. And it will provide theoretical and technical basis for rapid propagation and breeding.展开更多
The relationship between microspore developmental stages and morphological characteristics of floral organ in balsam pear was studied. The results showed that flower buds developed a series of morphological changes at...The relationship between microspore developmental stages and morphological characteristics of floral organ in balsam pear was studied. The results showed that flower buds developed a series of morphological changes at different microspore developmental stages, and morphological characteristics of floral organ were significantly different when sampling from different part or cultivar of balsam pear. Anther at late-uninucleate stage was best for culture, in which stage flower buds swelled obviously, and white floss on the surface reduced. In addition, calyxes were obvious but did not spread, and the most of anthers were light green.展开更多
Brown callus derived from anther limited the application of anther culture in balsam pear. In order to establish a more perfect regeneration system from anther cultuer, the effects of low temperature pretreatment, 2,4...Brown callus derived from anther limited the application of anther culture in balsam pear. In order to establish a more perfect regeneration system from anther cultuer, the effects of low temperature pretreatment, 2,4-dichlorophenoxyacetic acid （2,4-D）, vitamin C （Vc） and silver nitrate （AgNO3） on callus induction and browning in anther culture of balsam pear （Momordiea charantia L.） were investigated. The results showed that after pretreatment at 4 ℃ for 1 day, callus induction rate was the highest and browning rate was the lowest. Anthers on MS medium supplemented with 2,4-D 0.5 mg/L formed more and better callus. The medium supplemented with Vc or AgNO3 was advantageous to the induction of callus and reduction of browning. When cultured on medium supplemented with 50 mg/L Vc or 5 mg/L AgNO3, callus induction rate was the highest and browning rate was rather low.展开更多
The balsum pear （Momordica charantia L. ） anthers in the monokaryotic stage of microspore development were cultured in this experiment. Different Plant growth regulators＇ combinations, base media and carbon sources...The balsum pear （Momordica charantia L. ） anthers in the monokaryotic stage of microspore development were cultured in this experiment. Different Plant growth regulators＇ combinations, base media and carbon sources were studied for callus formation and organ differentiation from balsum pear anthers. The result showed that the best media for callus inducement was： MS＋BA 0.5 mg/L＋NAA 0.2 mg/L＋ 2, 4-D 0.5 mg/L＋KT 2.0 mg/L, with 3% sugar and 0.8% agar. The best media to induce roots from balsum pear anther callus was： MS＋NAA 0.05 rag/L＋ KT 0.5 rag/L, with 3% sugar and 0.8% agar. Most of adventitious roots from callus were triploid（2N=3X=33）展开更多
以新鲜苦瓜为原料,用复合溶剂提取苦瓜中叶绿素,并利用正交试验得出最佳提取条件为料液比1∶4.0,浸提时间3.0 h,浸提温度30℃,乙醇用量12.8 m L,石油醚用量30 m L时,苦瓜叶绿素提取率最高。经检测证明,在此条件下提取率最高可达0.021 5 ...以新鲜苦瓜为原料,用复合溶剂提取苦瓜中叶绿素,并利用正交试验得出最佳提取条件为料液比1∶4.0,浸提时间3.0 h,浸提温度30℃,乙醇用量12.8 m L,石油醚用量30 m L时,苦瓜叶绿素提取率最高。经检测证明,在此条件下提取率最高可达0.021 5 mg/g。展开更多
为获得珠江三角洲地区蔬菜中微量元素的合理用量,通过在2地进行3种蔬菜施镁、硼、钼的田间试验,研究三种元素不同配比对三种蔬菜的影响。结果显示:(1)不同品种的蔬菜对中微量元素的需求量不同;相同品种蔬菜在不同地点土壤上的施镁、硼...为获得珠江三角洲地区蔬菜中微量元素的合理用量,通过在2地进行3种蔬菜施镁、硼、钼的田间试验,研究三种元素不同配比对三种蔬菜的影响。结果显示:(1)不同品种的蔬菜对中微量元素的需求量不同;相同品种蔬菜在不同地点土壤上的施镁、硼、钼肥效应不同;相同蔬菜镁、硼、钼配施比单独使用的施用量可大幅度减少,尤其是硼和钼可减少50%。(2)在施氮磷钾基础上单独施用镁、硼、钼,菜心的Mg适施量约为13.350～15.750 kghm-2;B的适施量约为0.990～1.065 kghm-2;Mo的适施量为0.375～0.420 kg hm-2。蒜青的Mg适施量约为1.500 kg hm-2;B的适施量约为0.450 kg hm-2;Mo的适施量为0.240 kg hm-2。苦瓜的施硼适用量范围在1.050 kg hm-2左右。(3)在施氮磷钾基础上镁、硼、钼配合施用,菜心的Mg适施量约为12.000 kghm-2、B适施量约为0.450 kghm-2、Mo适施量约为0.240 kghm-2;苦瓜的Mg适施量约为12.000～18.000 kghm-2、B适施量约为0.450～1.800 kg hm-2、Mo适施量为0.120～0.240 kg hm-2;蒜青的B和Mo适用量分别为0.900 kg hm-2和0.210 kg hm-2、镁的施用量则应<1.350 kg hm-2。(4)镁和硼的施用量不仅受试验地土壤交换性镁和有效硼含量水平的影响,还与土壤pH值和蔬菜目标产量有关。展开更多
文摘The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear （Momordica charantia L.） were investigated. Sterilized for 6 rain with 0.1% （w/v） mercuric chloride solution is an essential sterilization method for stems. June and July proved to be better for material taking, followed by May. The callus formation rate of balsam pears reached the highest when medium added with IBA 1.0mg/L and BA 2.0 mg/L, while the optimum medium for adventitious bud induction was MS added with IBA 0.5mg/L and BA 4.0 mg/L. And it will provide theoretical and technical basis for rapid propagation and breeding.
文摘The relationship between microspore developmental stages and morphological characteristics of floral organ in balsam pear was studied. The results showed that flower buds developed a series of morphological changes at different microspore developmental stages, and morphological characteristics of floral organ were significantly different when sampling from different part or cultivar of balsam pear. Anther at late-uninucleate stage was best for culture, in which stage flower buds swelled obviously, and white floss on the surface reduced. In addition, calyxes were obvious but did not spread, and the most of anthers were light green.
文摘Brown callus derived from anther limited the application of anther culture in balsam pear. In order to establish a more perfect regeneration system from anther cultuer, the effects of low temperature pretreatment, 2,4-dichlorophenoxyacetic acid （2,4-D）, vitamin C （Vc） and silver nitrate （AgNO3） on callus induction and browning in anther culture of balsam pear （Momordiea charantia L.） were investigated. The results showed that after pretreatment at 4 ℃ for 1 day, callus induction rate was the highest and browning rate was the lowest. Anthers on MS medium supplemented with 2,4-D 0.5 mg/L formed more and better callus. The medium supplemented with Vc or AgNO3 was advantageous to the induction of callus and reduction of browning. When cultured on medium supplemented with 50 mg/L Vc or 5 mg/L AgNO3, callus induction rate was the highest and browning rate was rather low.
基金The authors would like to thank Dr. WANG Xiao-rong and DENG Qun-xian for their help in the tests. This study was supported by the Education Bureau Science Foundation of Sichuan Province （No. 2005A003） and the Sichuan Agricultural University Science Foundation （No. 3305）.
文摘The balsum pear （Momordica charantia L. ） anthers in the monokaryotic stage of microspore development were cultured in this experiment. Different Plant growth regulators＇ combinations, base media and carbon sources were studied for callus formation and organ differentiation from balsum pear anthers. The result showed that the best media for callus inducement was： MS＋BA 0.5 mg/L＋NAA 0.2 mg/L＋ 2, 4-D 0.5 mg/L＋KT 2.0 mg/L, with 3% sugar and 0.8% agar. The best media to induce roots from balsum pear anther callus was： MS＋NAA 0.05 rag/L＋ KT 0.5 rag/L, with 3% sugar and 0.8% agar. Most of adventitious roots from callus were triploid（2N=3X=33）
文摘为获得珠江三角洲地区蔬菜中微量元素的合理用量,通过在2地进行3种蔬菜施镁、硼、钼的田间试验,研究三种元素不同配比对三种蔬菜的影响。结果显示:(1)不同品种的蔬菜对中微量元素的需求量不同;相同品种蔬菜在不同地点土壤上的施镁、硼、钼肥效应不同;相同蔬菜镁、硼、钼配施比单独使用的施用量可大幅度减少,尤其是硼和钼可减少50%。(2)在施氮磷钾基础上单独施用镁、硼、钼,菜心的Mg适施量约为13.350～15.750 kghm-2;B的适施量约为0.990～1.065 kghm-2;Mo的适施量为0.375～0.420 kg hm-2。蒜青的Mg适施量约为1.500 kg hm-2;B的适施量约为0.450 kg hm-2;Mo的适施量为0.240 kg hm-2。苦瓜的施硼适用量范围在1.050 kg hm-2左右。(3)在施氮磷钾基础上镁、硼、钼配合施用,菜心的Mg适施量约为12.000 kghm-2、B适施量约为0.450 kghm-2、Mo适施量约为0.240 kghm-2;苦瓜的Mg适施量约为12.000～18.000 kghm-2、B适施量约为0.450～1.800 kg hm-2、Mo适施量为0.120～0.240 kg hm-2;蒜青的B和Mo适用量分别为0.900 kg hm-2和0.210 kg hm-2、镁的施用量则应<1.350 kg hm-2。(4)镁和硼的施用量不仅受试验地土壤交换性镁和有效硼含量水平的影响,还与土壤pH值和蔬菜目标产量有关。
