Characterization of Seed Storage Proteins in Eight Bambara Groundnut Landraces in Burkina Faso

The Bambara groundnut Vigna subterranea (L.) Verdc. is a drought-resistant indigenous African grain legume with significant nutritional and agronomic potential. This study aimed to characterize the seed storage proteins of eight Bambara groundnut landraces. Seeds of Bambara groundnut landraces were collected from local markets in Burkina Faso, and total soluble protein as well as protein fractions were extracted. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content of the seeds was found to be 18.46%, with variations ranging from 17.69% to 19.17% among the different landraces. Most of the protein content was soluble, constituting approximately 87.04% of the total crude protein. Albumin fraction was the most dominant, representing about 95.42% of the total soluble proteins. The globulin, prolamin and glutelin fractions accounted for 1.82%, 0.13% and 1.17% of the soluble proteins, respectively. The findings provide valuable insights into the protein composition of Bambara groundnut landraces and contribute to our understanding of its nutritional potential, laying the groundwork for further research on crop improvement and sustainable agriculture practices.

Physiological Resilience of Bambara Groundnut (Vigna subterranea L. Verdc) Genotypes to Intermittent Periods of Drought Stress at Different Growth Stages

Different genotypes of Bambara groundnut (Vigna subterranea L. Verdc) grow well under conducive environmental conditions, provided that adequate soil moisture is available during vegetative and reproductive phases. However, drought stress is the major limiting factor to bambara production, which accounts for up to 40% of yield losses. This situation could worsen due to drastic and rapid changes in the global climate. Landraces grown by farmers are low-yielding. Understanding the physiological response of different genotypes to drought stress is key to achieving food security through crop improvement and diversification. This study focused on variations in the response of Bambara groundnut genotypes to intermittent drought stress during the crop’s critical growth (vegetative and reproductive) stages. The experiment was undertaken at CSIR-Crops Research Institute Screen-house. The treatments were used in a factorial experiment with three replications in a randomized complete block design. The Bambara genotypes showed considerable variability in tolerance to drought stress. Drought stress during vegetative and reproductive stages significantly reduced crop growth indices, the leaf relative water content, chlorophyll content and leaf area. Drought stress during vegetative and reproductive stages had a more severe impact on the seed yield of genotype Nav Red, reducing it by 69% and 13%, respectively. Farmers should pay more attention to adopting drought-tolerant and high-yielding varieties for improved Bambara groundnut productivity and livelihoods.

Giant Asian honeybee or Bambara stings causing myocardial infarction,bowel gangrene and fatal anaphylaxis in Sri Lanka:a case series

The sting of Giant Asian honeybee(Apis dorsata) or Bambara in Sinhala and Kanmge Kulavi in Tamil is a common environmental hazard in Sri Lanka known to cause immediate allergic reactions,which could be fatal in sensitized individuals.We reported myocardial infarction, bowel gangrene and fatal anaphylaxis in a prospectively proven case series and the association of these uncommon complications with delayed removal of stingers from the patients’skin.

Influence of Cross-Inoculation on Groundnut and Bambara Groundnut-<i>Rhizobium</i>Symbiosis: Contribution to Plant Growth and Yield in the Field at Sarh (Chad) and Ngaoundere (Cameroon)

In the context of sustainable research aiming at improving seed yield and maintaining soil fertility, a study on cross-inoculation of groundnut (Arachis hypogaea L.) and Bambara groundnut (Vigna subterranean (L.) Verd.) with rhizobia strains from four crop legumes was carried out at Sarh (Chad) and Ngaoundere (Cameroon). The experiment was conducted in each site following a randomized complete block design with 6 treatments known as the control, and 5 cross-inoculation formulations (RA, RN, RS, RV and MR), each of which was replicated thrice. Results obtained show that cross-inoculation contributed to the improvement of growth (plant sizes and biomasses) of the two crop legumes, as well as their seed yield at harvest. The enhanced yield expressed in kg/ha varied from 9.83% to 63.73% for groundnut and 72.71% for Bambara groundnut. These findings suggest that groundnut and Bambara groundnut do have a symbiotic affinity with rhizobia from other crop legumes. Whereas Groundnut, Cowpea, Soybean, Bambara groundnut rhizobia (GR, CR, SR, BR) alone, and the fourth in a mixture (RM) could be used to help improving groundnut seed yields;this mixture would not be indicated for intensive cultivation of Bambara groundnut later.

Effect of Extrusion Cooking of White Yam (<i>Dioscorea rotundata</i>) and Bambara-Nut (<i>Vigna subterranean</i>) Blend on Some Selected Extrudate Parameters

The effect of thermo-extrusion processing of white yam (Dioscorea rotundata) and bambara nut (Vigna subterranean) on some selected extrudate parameters of residence time, throughput and moisture content were investigated in this study. Both yam and bambara nut were processed into meals of 750 μm and 500 μm respectively. Extrusion was carried out following a three variable response surface methodology using a Box Behnken design. Blend of yam meal and Bambara nut meal at ratio 80:20 respectively was conditioned into 12.5, 15.0 and 17.5% moisture content (dry basis) and allowed to equilibrate for 4 h. Extrusion was carried out on a single screw extruder at 50, 60 and 70 rpm screw speed and 130oC, 140oC and 150oC barrel temperatures. The study revealed that the extrusion time varied between 13.1 and 29.7 sec, throughput varied from 13.3 to 34.4 kg/h and product moisture ranged from 11.1 to 16.8% dry basis). All investigated extrudate parameters were significantly affected (p< 0.05) by the process variables of screw speed, barrel temperature and feed moisture content, and their interactions. The results were indicative that the second order polynomial was adequate to model the dependence of the extrudate parameters of residence time, throughput and moisture content on extrusion variables of barrel temperature, feed moisture content and screw speed.

Impact of <i>Cowpea mottle virus</i>on the Growth and Yield of Bambara Groundnut (<i>Vigna subterranean</i>(L.) Verdc.)

Bambara groundnut [Vigna subterranean (L.) Verdc] is a food legume with high potential for food security in Sub-Saharan Africa. However, in addition to being a neglected crop, its production is limited by several constraints among which viral diseases are most cited. In order to contribute to the improvement of Bambara groundnut in Burkina Faso, local accessions of the crop were screened for resistance to Cowpea mottle virus (CPMoV), one of the most damaging viruses in grain legumes. Seven local accessions (C1 to C7) from two agro-ecological zones were evaluated by mechanical inoculation in field conditions in 2016 and 2017. The infected plants exhibited various symptoms of chlorosis, leaf deformation, growth retardation and plant stunting. CPMoV caused a significant reduction in the number of flowers and pods. As a result, grain yield was reduced by 49.5% to 83.9% depending on the accessions. The impact of the virus in yield loss was lowest in accessions C6 and C7 which indicated their possible used in the management of Cowpea mottle virus disease in bambara groundnut.

Effects of replacement of soya bean meal by bambara nut sievate on the carcass and organ parameters of finisher broiler chicks

Eighty 4-weeks old broiler chicks (Anak 2000 strain) were used in a 28 days feeding trial to determine the effects of replacement of soya bean meal (SBM) by bambara nut sievate (BNS) on the carcass and organ characteristics of fin- isher broiler chicks. BNS replaced soya bean meal by weight for weight at levels 0%, 5%, 10% and 15% for treatments 1,2, 3 and 4;respectively which were replicated four times in a completely randomized design. Feed and potable water were supplied ad libitum to the birds. Also, ap- propriate medication, sanitation and other stan- dard management practices were strictly adopt- ed. At the 28th day, one bird was randomly picked from each replicate, starved of food for 24 hours and then slaughtered and eviscerated for carcass and organ evaluation. Weights were measured with digital weighing scale. Results of analysis of variance of carcass parameters showed significant (P 0.05) in the values above but only varied with birds on 10% and 15% BNS. Other carcass parameters were simi- lar (P > 0.05) in value between treatments. The liver, heart, gizzard and intestine significantly varied (P < 0.05) between their treatments. How- ever, kidney values did not differ significantly. From the results, it appeared that 5% BNS in- clusion is optimal for carcass and organ char- acteristics of finisher broilers.

Assessment of Microbial Changes and Nutritional Qualities of Extruded White Yam (<i>Dioscorea rotundata</i>) and Bambara Groundnut (<i>Vigna subterranean</i>) Blends

Two flavoured extruded products were developed by co-extruding yam grits (750 μm) obtained from white yam (Dioscorea rotundata) and bambara groundnut flour (250 μm) in 160:40 respectively obtained from white bambara groundnut with added flavouring agents of salt (1% - 3%) and sugar (4% - 6%) in the feed blends at screw speed of 70 rpm, 17.5%, feed moisture and at the barrel temperature of 145℃. The extruded products were packaged in low density polyethylene bag (0.02 μm gauge size) and stored at room temperature (28℃ ± 2℃) and at refrigeration temperature (9℃ ± 2℃) for a period of twenty weeks. The microbiological changes in the extruded products as determined by the total plate under both storage conditions showed that maximum total plate counts were 0.5 × 104 and 5.4 × 104 cfu/g at 9℃ ± 2℃ and 28℃ ± 2℃ respectively. Nutritional evaluation studies of extrudates were comparable (p ≥ 0.05) with standard casein diet with minimum crude protein content of 13.51% providing 1707.2 KJ energy per 100gof diet and supported weight gain and growth of laboratory animals.

Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (<i>Vigna subterranea</i>(L.) Verdc.) in Burkina Faso

Potyviruses are major constraints to grain legume production by causing significant yield losses. Potyviruses infecting Bambara groundnut (Vigna subterranea) were investigated in Burkina Faso. Leaf samples collected from three agroclimatic zones were subjected to RT-PCR and sequence analyses. Of a total of 135 samples, 36 (26.67%) were detected positive in RT-PCR tests using potyvirus universal primers. Analysis of full coat protein (cp) sequences from 24 isolates revealed the occurrence of three groups of Bambara groundnut-infecting potyviruses. Virus isolates in group 1 shared 94.5% - 100% nucleotide (nt) identity with CABMV whereas those in group 2 and group 3 were distantly related Bean common necrosis virus (BCMNV) and Passiflora virus Ugandan which were their respective closest potyviruses. Group 2 shared 77.1% nt and 78.8% - 79.9% aa identity with BCMNV and group 3 shared 77.3% - 78.3% nt and 80.7% - 81.5% aa identity with Passiflora virus Ugandan. All three groups were confirmed by phylogenetic analyses. Taking into account potyvirus demarcation criteria, group 1 isolates belonged to CABMV species. Group 2 and group 3 were assigned to a potentially new potyviruses species and designated Bambara groundnut potyvirus 1 (BGPV1) and Bambara groundnut potyvirus 2 (BGPV2).

Enhancing the Productivity and Sustainability of Bambara Groundnut (Vigna subterranea (L.) Verdc.) Production Using Inorganic Phosphorus Fertilizer

Phosphorus (P) is a vital element required for nodulation, stomatal regulation and photosynthesis in legume crops. P-deficiency in tropical soils limits the growth and productivity of Bambara groundnuts. The current study focused on determining the potential suitability of underutilized crops for food security using phosphorus fertilizer as soil amendment practice. A field trial was carried out at the Council for Scientific and Industrial Research—Crops Research Institute (CSIR-CRI), over two growing seasons to determine the optimum P rate for Bambara production. This trial was laid out in a split plot in a randomized block design with three replications. Bambara genotypes represented the main plots while four P fertilizer rates (0, 30, 45 and 60 kg P2O5 ha-1) were the sub-plots. The appropriate application rate of 60 kg P2O5 ha-1 showed excellent performance based on growth and yield analysis, and the results indicate a positive significant interaction between landraces and phosphorus fertilizer rates. The biological suitability of 60 kg P2O5 ha-1 increased the number of nodules per plant for Tiga Necuru, Kenya Capstone and Nav Red by 42.8%, 51.3% and 42.1% respectively, over control plots. The same for pod yield is 12%, 28% and 52% significantly higher than when P was applied at 45, 30 and 0 kg P2O5 ha-1 respectively. The results further revealed that on days to flowering and maturity, the plant height, the number of branches and dry matter increased significantly at each level of P fertilizer rate applied. Bambara production at 0 kg P fertilizer rate might not be sufficient to enhance Bambara productivity significantly. The outcome of this study reveals the suitability of phosphorus fertilizer application in enhancing the sustainability of Bambara groundnut productivity and the potential of Bambara in diversifying crop production to ensure food security.

Alectra vogelii: A Threat to Bambara Groundnut Production under Climate Change: A Review Paper

Bambara groundnut (BGN) is a protein-rich pulse with the ability to lead to more climate-resilient agriculture. The objective of this study was to review Alectra vogelii as a potential threat to BGN production as a result of climate change. However, the crop faces biotic and abiotic stresses. Alectra vogelii is a major biotic constraint to BGN production, especially in Africa’s non-fertile semi-arid regions. Alectra vogelii (L.) Benth is a parasitic weed in the Orobanchaceae family that causes major damage by forming haustoria attached to roots to enable absorption of nutrients from the BGN. Alectra vogelii produces a large number of minute seeds that can live in the soil for up to 20 years. Based on the reviewed literature, various control mechanisms for dealing with the harmful effects of Alectra vogelii have been proposed. The aim of this research was to reveal the effect of Alectra vogelii on BGN and possible control strategies. We discuss the different control methods such as cultural and mechanical management procedures, phosphorus fertilizers and resistant host crops, herbicide use, and integrated Alectra vogelii control methods. In adaptive methods, however, new techniques remain important. The life cycle of root parasitic weeds is inextricably linked to that of their host, making it an ideal target for such new control techniques, especially when aimed at the early stages of the host-parasite relationship. This review reveals additional information on the function of parasitic seed, strigolactones and how they can be used in breeding to management parasitic weeds.

Molecular Characterization Using Microsatellites of Bambara Nut (<i>Vigna subterranea</i>[L] Verdcourt) Landraces Cultivated in Burkina Faso

Voandzou is a seed legume cultivated in Burkina Faso with significant nutritional potential. The objective of this study was to assess the genetic diversity of Bambara nut cultivated in Burkina Faso using microsatellite markers. For the study, fifteen SSRs markers were used for molecular characterization of 90 Bambara nut landraces from three agro-climatic zones of Burkina Faso. All markers were 100% polymorphic with an average value of 4.81 for effective alleles. The polymorphism information content (PIC) ranged from 0.654 to 0.867 with a mean of 0.775. Dendrogram classified the accessions in four mixed groups independently of the three agro-climatic zones. This distribution is consistent with the results on the agro-morphological characterization of the same landraces. This information was served as a basic model for breeding and conservation programs of V. subterranea in Burkina Faso.

Resistance to Abiotic Stress and Effectiveness of Native Rhizobia on Bambara Groundnut [Vigna subterranea (L.) Verdc.] in Benin

Bambara groundnut [Vigna subterranea (L.) Verdc.], as a legume, can establish relationships with nitrogen-fixing bacteria such as Rhizobium. However, Rhizobium efficacy is not always optimal due to the lack or poor efficient strains in the soil. This study aimed to evaluate symbiotic efficiency of endogenous Rhizobia nodulating Bambara groundnut and their resistance to abiotic conditions. Root nodules were randomly sampled from three agroecological zones across the country, surface sterilized, ground and paste plated on YEMA media. After 24 hours, the bacterial colonies were purified. The pure cultures were further characterized using morphological and biochemical methods and their resistance to antibiotics and heavy metals was evaluated. Lastly, the symbiotic efficiency of the isolates was assessed through a greenhouse experiment. A total of eighty-five presumptive strains were isolated from Bambara groundnut roots nodules obtained from the farms. The physiological characterization of the isolated showed a decrease in isolates growth when NaCl concentration was more than 7%. In addition, 47% of the isolates were tolerant to a temperature of 40°C. Most of the isolates were highly resistant to Erythromycin in all its concentration levels and to Kanamycin, Spectinomycin, Neomycin and Ampicillin at 10 μg⋅mL−1. Most of them showed resistance to Cu and Zn at 10 μg⋅mL−1. Results of the effectiveness test on two Bambara groundnut varieties yielded dry shoot matter varying from 3.33 g⋅plant−1 to 7.21 g⋅plant−1 for variety 1 and from 4.38 g⋅plant−1 to 8.38 g⋅plant−1 on variety 2. N uptake ranged between 0.09 g⋅plant−1 and 0.29 g⋅plant−1 for variety 1 and between 0.12 and 0.29 g⋅plant−1 for variety 2. The isolates yielding higher shoot dry weight and N uptake were LMSEM312, LMSEM338, LMSEM307, LMSEM351 for variety 1 and LMSEM338, LMSEM309, LMSEM307 for variety 2. The isolates showing better performance can be used to develop bio-fertilizer for sustainable Bambara groundnut production in Benin.

Sentiment Analysis of Code-Mixed Bambara-French Social Media Text Using Deep Learning Techniques

The global growth of the Internet and the rapid expansion of social networks such as Facebook make multilingual sentiment analysis of social media content very necessary. This paper performs the first sentiment analysis on code-mixed Bambara-French Facebook comments. We develop four Long Short-term Memory（LSTM）-based models and two Convolutional Neural Network（CNN）-based models, and use these six models, Na？ve Bayes, and Support Vector Machines（SVM） to conduct experiments on a constituted dataset. Social media text written in Bambara is scarce. To mitigate this weakness, this paper uses dictionaries of character and word indexes to produce character and word embedding in place of pre-trained word vectors. We investigate the effect of comment length on the models and perform a comparison among them. The best performing model is a one-layer CNN deep learning model with an accuracy of 83.23 %.

Attenuation of oxidative stress and hepatic damage by some fermented tropical legume condiment diets in streptozotocin-induced diabetes in rats

Objective:To investigate the modulatory effect of fermented legume condiments diet on oxidative stress in streptozotocin(STZ) induced diabetic rats.Methods:Adult male Wistar rats were randomly divided into six groups with six animals in each group.Diabetes was induced by intraperitoneal injection of STZ(35 mg/kg b.w.).After being confirmed diabetic,the rats were fed with fermented Bambara groundnut.Locust bean and Soybean diets for 14 days.The plasma was obtained after 14-day treatment and analyzed for hepatic damage marker enzymes(AST,ALT and ALP) and in vivo antioxidant indices.Results:The diabetic untreated rats showed elevated (P【0.05) levels of AST,ALT.ALP and malondialdehyde with reduced activities of glutathiones -transferase,catalase as well as plasma reduced glutathione,vitamin C and total protein content.However,treatment of diabetic rats with lermenled legume condiments diets for 14 days significantly(P【0.05) reversed the above parameters towards normalcy,suggesting their modulation of oxidative stress,which mav be due to their high phenolic content and antioxidant capacity.Conclusions:The attenuation of oxidative stress and protection of hepatic tissue damage by the legume condiment diets in STZ induced diabetic rats compare favourably with that of metformin,a well known oral hypoglycemic drug.

生命不可承受之选择——《爵士乐》和《吃盐者》中身份构建之对比研究

莫里森的《爵士乐》和班芭拉的《吃盐者》中截然不同的文化视角为20世纪末的美国黑人群体提供了两种可选的社会政治范式。在探讨个体身份构建的途径、剖析过去展望未来的过程中,莫里森呼吁她的人民从过去身份的牢笼中解放出来,参与到无限的选择中去;班芭拉则认为背负历史的重任,在集体创造的过程中构建自我才是惟一的选择。

集体观照下的身份选择与构建——解读班芭拉的小说《吃盐者》

多元文化背景下少数族裔如何建立起自身独特的文化身份认同,是美国当代黑人女作家班芭拉在长篇小说《吃盐者》中提出的关键问题。对此她给出的解答是,在主体性身份的构建过程中,明确个人的自由与责任,建立与本民族文化的联系才是唯一正确的选择。

Production and Quality of Biscuits from Composite Flours

Biscuits were produced from bambara nut,cowpea and wheat flour blends.This study was carried out to evaluate the effects of varying the proportions of these flours on the nutritional quality and general acceptability of the biscuits.Five blends of composite flours were prepared by mixing wheat,bambara nut and cowpea(WBC)flours in the proportions:T1(90:5:5),T2(80:10:10),T3(70:15:15),T4(60:20:20),T5(50:25:25)and 100%whole wheat flour was used as the control(T0).Composite flours produced were subjected to functional and proximate analysis while the biscuits made from the flour blends were also subjected to sensory evaluation and proximate analysis.The use of composite flour for the production of biscuits from cowpea and bambara nut flours as supplement for the wheat flour has improved the nutritional contents of the biscuits with protein value being highest at mixing ratio T5.The functional properties of the biscuits showed some significant differences(p<0.05)when compared with whole wheat biscuits.The results showed that biscuits produced from T5 formulation with proximate composition of 11.87%moisture,2.90%ash,18.61%protein and 75.62%carbohydrate was selected as the best product.

治疗美国黑人两性关系:对《吃盐的人》的文化解读

本论文以小说《吃盐的人》中的薇尔玛和两位男性人物詹姆斯及弗莱德在黑人性别话语中的表现为研究对象,运用文化剖析的策略细读文本,旨在揭示美国黑人男女两性关系存在着严重的危机,以及白人统治的社会语境所造成的社会偏见与文化创伤。本文认为通过“文化治疗”实现黑人的两性和谐已成为整个黑人群体无法回避的现实选项。

摩洛哥哈穆利亚格纳瓦音乐之初探

本文以摩洛哥东南部村庄哈穆利亚的格纳瓦音乐为研究对象,通过两次赴摩洛哥的实地采风,对其艺术价值和宗教意义作出了较为客观的评价。摩洛哥既受到阿拉伯文明的影响,又与撒哈拉沙漠以南的黑非洲地区有着不可割舍的关系,同时,由于挨近欧洲南部,其文化又不免接受西方的输入。这种多文化的融合也自然可在其宗教音乐中窥探出来。而格纳瓦(Gnawa)正是这种融合的一个典型体现。位于摩洛哥东南部,紧邻撒哈拉沙漠尔格·切比(Erg Chebbi,阿拉伯文:■■)的村庄哈穆利亚是格纳瓦在摩洛哥的三个中心之一。笔者分别对哈穆利亚村庄的两个格纳瓦组织、一个节日进行了深入的走访、调查与分析,下文将对其所处的尔格·切比沙漠、村庄本身、族群、组织、音乐进行细微描述。